Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F11 I3 R1 74 850.3 37733610 99.3% 37469474 136.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451_phaM1 5,555,608 +GCC intergenic (‑84/+87) PP_4887 CDS (conserved protein of unknown function) ← / ← PP_4888 CDS (putative methyl‑accepting chemotaxis transducer) –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM15,555,6081.G90.3% 740.2 / 93.6 266intergenic (‑84/+87)PP_4887 CDS (conserved protein of unknown function)/PP_4888 CDS (putative methyl‑accepting chemotaxis transducer)–/–
Reads supporting (aligned to +/- strand):  ref base . (0/25);  new base G (7/225);  total (7/259)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*AE015451_phaM15,555,6082.C90.5% 840.9 / 86.1 266intergenic (‑84/+87)PP_4887 CDS (conserved protein of unknown function)/PP_4888 CDS (putative methyl‑accepting chemotaxis transducer)–/–
Reads supporting (aligned to +/- strand):  ref base . (0/25);  new base C (5/234);  total (7/259)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*AE015451_phaM15,555,6083.C80.0% 774.4 / 208.0 266intergenic (‑84/+87)PP_4887 CDS (conserved protein of unknown function)/PP_4888 CDS (putative methyl‑accepting chemotaxis transducer)–/–
Reads supporting (aligned to +/- strand):  ref base . (0/53);  new base C (6/206);  total (7/259)
Fisher's exact test for biased strand distribution p-value = 6.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 273 total aligned reads displayed.

TGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑G‑‑‑CC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAG  >  AE015451_phaM1/5555472‑5555727
                                                                                                                                                |||                                                                                                                        
tgCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑g‑‑‑c‑g                                                                                                                             <  2:14603725/137‑1 (MQ=255)
tgCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAACATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑g‑‑‑c‑g                                                                                                                             <  2:4982207/137‑1 (MQ=255)
    ggAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑g‑‑‑c‑ggccc                                                                                                                         <  2:6273480/137‑5 (MQ=255)
    ggAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑g‑‑‑c‑ggccc                                                                                                                         <  2:11980759/137‑5 (MQ=255)
      aaaTTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑g‑‑‑c‑ggccccg                                                                                                                       <  1:11597054/137‑7 (MQ=255)
      aaaTTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑g‑‑‑c‑ggccccg                                                                                                                       <  1:9433781/137‑7 (MQ=255)
              gTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTc                                                                                                              <  2:16649389/137‑1 (MQ=38)
                cGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCcg                                                                                                            <  1:12013006/137‑1 (MQ=38)
                 ggCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCcgc                                                                                                           <  2:14065069/137‑1 (MQ=38)
                   ccTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATNTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCgt                                                                                                         <  1:15263227/137‑1 (MQ=38)
                   ccTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCgt                                                                                                         <  1:2836237/137‑1 (MQ=38)
                   ccTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCgt                                                                                                         <  1:10253029/137‑1 (MQ=38)
                   ccTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCgt                                                                                                         <  1:7914000/137‑1 (MQ=38)
                   ccTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCgt                                                                                                         <  2:12999478/137‑1 (MQ=38)
                   ccTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCgt                                                                                                         <  2:13764433/137‑1 (MQ=38)
                   ccTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCgt                                                                                                         <  2:15055900/137‑1 (MQ=38)
                   ccTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCgt                                                                                                         <  2:18399221/137‑1 (MQ=38)
                   ccTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCgt                                                                                                         <  2:3114110/137‑1 (MQ=38)
                   ccTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCgt                                                                                                         <  2:4579757/137‑1 (MQ=38)
                   ccTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCgt                                                                                                         <  2:5459637/137‑1 (MQ=38)
                   ccTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCgt                                                                                                         <  1:2129009/137‑1 (MQ=38)
                   ccTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCAGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCgt                                                                                                         <  1:3579851/137‑1 (MQ=38)
                     tGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCgtgt                                                                                                       <  2:16822654/137‑1 (MQ=38)
                     tGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCgtgt                                                                                                       <  2:9221960/137‑1 (MQ=38)
                     tGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCgtgt                                                                                                       <  1:7933542/137‑1 (MQ=38)
                         cAGCGATTAGTCGGGCGGACATGAGCAACTCNTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGc                                                                                                   <  2:665927/137‑1 (MQ=38)
                         cAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGc                                                                                                   <  1:14993769/137‑1 (MQ=38)
                         cAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGc                                                                                                   <  2:2504568/137‑1 (MQ=38)
                         cAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGc                                                                                                   <  2:7106750/137‑1 (MQ=38)
                         cAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGc                                                                                                   <  2:16005922/137‑1 (MQ=38)
                         cAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGc                                                                                                   <  1:13835926/137‑1 (MQ=38)
                          aGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGca                                                                                                  <  1:5179186/137‑1 (MQ=38)
                            cGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGcaac                                                                                                <  2:16962246/137‑1 (MQ=38)
                              aTTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGcaacaa                                                                                              <  1:8942799/137‑1 (MQ=38)
                              aTTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGcaacaa                                                                                              <  1:9419740/137‑1 (MQ=38)
                                tAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAAc                                                                                            <  2:15283664/137‑1 (MQ=38)
                                 aGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAAcc                                                                                           <  1:12082595/137‑1 (MQ=38)
                                 aGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAAcc                                                                                           <  2:10294511/137‑1 (MQ=38)
                                 aGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAAcc                                                                                           <  2:18223768/137‑1 (MQ=38)
                                   tCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTg                                                                                         <  2:16699189/137‑1 (MQ=38)
                                   tCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTg                                                                                         <  2:15512896/137‑1 (MQ=38)
                                   tCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTg                                                                                         <  1:3332208/137‑1 (MQ=38)
                                    cGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgg                                                                                        <  1:1055324/137‑1 (MQ=38)
                                    cGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgg                                                                                        <  1:10928196/137‑1 (MQ=38)
                                      tgCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATGGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                      <  2:9416703/136‑1 (MQ=38)
                                      ggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCTCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                      <  1:3815729/137‑1 (MQ=38)
                                      ggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                      <  1:17022219/137‑1 (MQ=38)
                                      ggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                      <  1:10039462/137‑1 (MQ=38)
                                      ggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                      <  1:1605578/137‑1 (MQ=38)
                                      ggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                      <  1:12478710/137‑1 (MQ=38)
                                      ggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                      <  1:5950758/137‑1 (MQ=38)
                                      ggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                      <  2:13969733/137‑1 (MQ=38)
                                      ggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                      <  1:15104290/137‑1 (MQ=38)
                                      ggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                      <  1:633771/137‑1 (MQ=38)
                                      ggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                      <  1:8039380/137‑1 (MQ=38)
                                      ggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                      <  1:14575738/137‑1 (MQ=38)
                                            caggAGCAATTCCTTTTGTTATGGGTCGCCGACAGCATAGTAACACCACTCATGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATGTCCGCGTGTATGCAACAAACCTGGGGCTGCTa                                                                                <  2:1259931/134‑1 (MQ=25)
                                            cATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTa                                                                                <  2:8519142/137‑1 (MQ=38)
                                                        cTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCATC‑‑‑G‑‑‑C‑GGGCCC‑GGTGATTTTCGCGTGTGTGTAAAAAACCTGGGGGTACTCTGTAGCACtttt                                                                    >  2:15551272/1‑136 (MQ=19)
                                                             ttCATGTGTCGCCGACAGCATAAAAACACCGCTAGGGTGGGGAAGCGGCCTTGTTTCGCGAAAGGGCCGCATAGTGG‑‑‑C‑C‑‑‑CC‑GGCAATTTCCGTGTGTGTGCAACAAACCTGGGGCTGCTGTGCAGCCCTTTCGCGac                                                               <  2:14864456/136‑1 (MQ=25)
                                                             tcaaTGGGTCGCTGACAGCGGAGCACCACCCCTCAGGTGTGGGAGCGTCCTTGTGCCGCGAAGGGGCCGCAGA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAAAAAACCTGGGGCTGCTGTGCAGCCCTTTCGCGac                                                               <  2:11587056/134‑1 (MQ=19)
                                                               cATGTTTCGCCGACAGAATAGCAACACCATTCAGGTGTGGGAGCGGCCTTGTGTCGCAAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCACGTGTATGCAACAAACCTGGGGCGGCGATGCAGCCCTTTCGCGacac                                                             <  2:4868744/137‑1 (MQ=25)
                                                                 tgttTCGCCGACAGCACAGCAACACCTCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCGTA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACaa                                                           <  2:10781321/137‑1 (MQ=37)
                                                                 tgtgTCTCCGACGGCATAGAAACACCGCTCAGGTGTGGGAGCGGCCTTGTTTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGTGTGGATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACaa                                                           <  2:4376702/137‑1 (MQ=25)
                                                                 tgtgTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAGGGGCAGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACaa                                                           <  2:7729934/137‑1 (MQ=38)
                                                                 ggtgtCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCAGA‑‑‑G‑‑‑C‑GGC‑CCTGGCAATTTCCGCGTGGATGCAAAAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACaa                                                           <  2:2233303/136‑1 (MQ=25)
                                                                  ttgtCGCCGACAGCATAGCAACACCGCTCGGGTGTGGGAGTGACCTTGTGTCGCGAAAGGTCCGCATG‑‑‑G‑‑‑C‑GGCCCC‑GACAGTTTCCGCGTGTATGCAACAAACTTGGTGTTGCTGTGCAGCCCTTTCGCGACACAAg                                                          <  2:4264238/136‑1 (MQ=255)
                                                                      cGCCGACAGCAAAACAACCCCCTTCAGGTGGGGGAGCGGCTTTGTGTCGGGAAAGGGCCGAAGA‑‑‑G‑‑‑G‑GGC‑CCAGGCAGTTTCCGCGGGTATGAAACAAACTTGGGGTTGCTATGCAGCCCTTTCGCGACACAAGGccc                                                      <  2:14280234/137‑1 (MQ=255)
                                                                                agaacAACACCGCTCAGGTGGGGGAGTGGCTTGGTGTAGAGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGAAATTTCCGTGTGTGGGAAACAAACCTGGGGCTGCTGTGCAGCCCTTTCGCGACACAAGGCCCACAAAGGAcc                                            <  2:13827636/133‑1 (MQ=19)
                                                                                acaGCACCACCTCTCAGGTGTGGGAGCGGCCTTGTTTCGCGAAAGGGCCGAATG‑‑‑GTGAC‑C‑‑‑CC‑GGAAGTTTCCGCGGGGATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGAcc                                            <  2:10919311/135‑1 (MQ=25)
                                                                                aTAGCAACCCCTCTCAGTTGTGGGAGTGGCTTTGTTTCGCGAAAGGGCTGAATG‑‑‑G‑‑‑T‑GGC‑CCTGGCACTTTCCGCTTGCATGCAACAACCCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGAcc                                            <  2:11816472/137‑1 (MQ=255)
                                                                                aTAGCAACACCTCTCAGGTGTGGAAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTTGCGACACAAGGCCCACAAAGGAcc                                            <  2:18190858/137‑1 (MQ=37)
                                                                                aTAGAAACACCGCTAGGGTGGGGGGGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑GTGTC‑C‑‑‑CC‑GGAAATTTCTGTGTGGAGGAAAAAAACTTGGGGTGGTCATGCATCCTTTTCGCAACACAAGGCCCACAAAGGAcc                                            <  2:10576481/137‑1 (MQ=255)
                                                                                  agaaaCACCTCTCAGGTGTGGGAGCGGCTTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGTGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACcgc                                          <  2:11454884/134‑1 (MQ=25)
                                                                                  aacAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATATCGG‑‑‑C‑C‑‑‑CC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACcgc                                          <  2:4436433/135‑1 (MQ=38)
                                                                                  aGCAACACCTCTCAGTTGGGGGAGCGGCCTTTTGTCGCGAAAGGGCCGCAGA‑‑‑G‑‑‑T‑GGCCCC‑GGCACTTTCCGCGTGTATGCAACAACCCTGGGGCTGTGATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACcgc                                          <  2:9844749/137‑1 (MQ=25)
                                                                                  aGCAACACCGTTCGGGTGTGGGGGTGGCTTTGTGTTGCGAAGGGGCCGCATG‑‑‑G‑‑‑T‑GGCCCC‑GGCAGTTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACcgc                                          <  2:3470507/137‑1 (MQ=255)
                                                                                  aGCAACACCGTCCAGGTGGGGGAGCGGCCTTGTTTCGCAAAGGGGCTTCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCACTTTCCGCGGGCAGAAAACAAACCTGGGGCTGCTATGCGGCCCTTTCGCGACACAAGGCCCACAAAGGACcgc                                          <  2:2933258/137‑1 (MQ=255)
                                                                                  aGCAACACCGCTCAGGTTTGGGAGCGGCCTTGTGTCGCAAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCGGCTATGCAACCCTTTCGCGACACAAGGCCCACAAAGGACcgc                                          <  2:7467381/137‑1 (MQ=37)
                                                                                  aGCAACACCGCTCAGGGGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAGTTTCCGCGTGGATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACcgc                                          <  2:17517639/137‑1 (MQ=37)
                                                                                       cacCGCTCAGGTGTGGGGGGGGCCTTGTGTCGCGAAGGGGCAGAATA‑‑‑G‑‑‑C‑GGCCCC‑GGCACTTTCCGTGTGTAGGCAACAAACCGGGGGTTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAgg                                     <  1:15762797/137‑1 (MQ=25)
                                                                                        acCGCTCAGGTGGGGGAGCGGCCTTGTGTCGCGAAAGGTCCGCGTA‑‑‑G‑‑‑C‑GGCCCC‑GGCAGTTTCCGTGTGTATGCAACAAACCTGGGGCTGCTATGCATCCTTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGc                                    <  2:6466056/137‑1 (MQ=25)
                                                                                             tCAGGTGTGGGAGCGGCCTTGTGTTGCGAAAGGGCCGCATA‑‑‑T‑‑‑C‑GTCCCC‑GGCAATTTCCGCGTGTATGCAAAAAACCTGGGGTTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAg                               <  2:16434041/137‑1 (MQ=37)
                                                                                             tCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAGTTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAg                               <  2:8380859/137‑1 (MQ=37)
                                                                                                  tgtgGGAGTGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACTTGGGGCTGCTATGCAGCCCTTTCTCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCg                          <  1:13486052/137‑1 (MQ=37)
                                                                                                  tgtgGGAGCGGCTTTGTGTCGCGAAAGGGCCGCATAGTGG‑‑‑C‑C‑‑‑CC‑GGCAGTTTCCGCGTGTAGGAAACAAACCTGGGGCGGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCg                          <  2:1888200/137‑1 (MQ=37)
                                                                                                  tgtgGGAGCGGCTTTGTGCCAGAAGAGGGCCGCAGAGAGG‑‑‑C‑C‑‑‑CC‑GGAAGTTTCACCGGGTATGCAAAAAACTTGGGGTTGCTATGAGGCCTTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCg                          <  2:12299493/137‑1 (MQ=255)
                                                                                                  tgggggATCGGCCTTGTGTCGCGAAAGGGCCGCGGA‑‑‑G‑‑‑C‑GGC‑CCAGGCAATTTCCGAGTGTGTGCAACAAACCTGGGGCTGCTGGGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCg                          <  2:12861525/134‑1 (MQ=25)
                                                                                                  ggggggAGCGGCCTTGGGTAGAGAAGGGCCCGCAGA‑‑‑G‑‑‑C‑GGC‑CCAGTCAGTTTCCGTGTGTATGCAACAAACTTNTGGTTGCTATGCAGCCCTTTCCCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCg                          <  1:17403120/134‑1 (MQ=255)
                                                                                                   ttgGGAGCGGCTCTTTGTCGCGAAAGGGCTGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTCCCGCGGGTATGCAACAAACTTGGGGCTGCTATGCACCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCgg                         <  2:15919102/136‑1 (MQ=255)
                                                                                                   gtgGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGC‑CCTGGCAATTTCCGTGTGTAGGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCgg                         <  2:10612428/137‑1 (MQ=37)
                                                                                                        aGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGAAACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTg                    <  2:2072253/137‑1 (MQ=38)
                                                                                                             ctttGTTTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑CTGCC‑CC‑GGCAATTTCCGCGTGTATGCAACAAACCTTGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCa               <  2:18126712/135‑1 (MQ=255)
                                                                                                             ccTTTTGTCGCGAAGGGGCCGAATATT‑GT‑‑C‑C‑‑‑CT‑GGCATTTTCCGCTTGTATGCAACAACCTTGGGGCTTCGGTACATCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCa               <  1:18311082/137‑1 (MQ=255)
                                                                                                                  tgtagagAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGC‑CCTGGCATTTTCCGTGTGTAGGCAACAAACTTGGGTCTGCTATGCATCCCTTTCGCAACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTg          <  1:9978748/131‑1 (MQ=255)
                                                                                                                  tgtCGCGAGGGGGCCGCATA‑‑‑G‑‑‑C‑GGC‑CCTGGCACTTTCCGTGTGTGTAAAACAACCTTGGGTCTGCTATGCATCCTTTTCTCAACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTg          <  1:7531626/137‑1 (MQ=255)
                                                                                                                  tgtCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGCATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTg          <  2:6824878/137‑1 (MQ=38)
                                                                                                                  tgtCGCGAAAGGGCCGCAGA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTAGGAAACAAACTTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTg          <  1:18761518/137‑1 (MQ=37)
                                                                                                                          aaGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCCAAAAGGAACGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAg  >  2:8020629/1‑137 (MQ=255)
                                                                                                                          aaGGGCCGCATA‑‑‑G‑‑‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGATGCTCTGCAGCCCTTTTTCGAAACAAAGCCCACAAAGGACCGCGCAGGATTCCGGCCCGGAACTGGCCCAGGCTGGTGCGCAg  >  2:10960442/1‑137 (MQ=255)
                                                                                                                                                |||                                                                                                                        
TGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATA‑‑‑G‑‑‑C‑G‑‑‑CC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAG  >  AE015451_phaM1/5555472‑5555727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCG‑‑‑CCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCTTGC  >  AE015451_phaM1/5555459‑5555756
                                                                                                                                                                  |                                                                                                                                                       
TACGCTGTCACTGTGCAGGAAATTCAGTTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCG‑‑‑G                                                                                                                                                     <  LH00941:114:22FVMWLT1:2:1239:10060:29183/151‑1 (MQ=60)
TACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTATTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCG‑‑‑G                                                                                                                                                     <  LH00941:114:22FVMWLT1:2:1126:8368:9663/151‑1 (MQ=60)
TACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCG‑‑‑G                                                                                                                                                     <  LH00941:114:22FVMWLT1:2:2254:8673:4010/151‑1 (MQ=60)
TACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCG‑‑‑G                                                                                                                                                     <  LH00941:114:22FVMWLT1:2:2254:8766:2857/151‑1 (MQ=60)
TACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCG‑‑‑G                                                                                                                                                     <  LH00941:114:22FVMWLT1:2:1126:8378:9647/151‑1 (MQ=60)
TACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCG‑‑‑G                                                                                                                                                     <  LH00941:114:22FVMWLT1:2:2137:38914:7069/151‑1 (MQ=60)
TACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCG‑‑‑G                                                                                                                                                     <  LH00941:114:22FVMWLT1:2:1234:40781:28750/151‑1 (MQ=60)
 ACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCG‑‑‑GC                                                                                                                                                    <  LH00941:114:22FVMWLT1:2:1254:31037:3642/151‑1 (MQ=60)
  CGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGgcc                                                                                                                                                      <  LH00941:114:22FVMWLT1:2:1256:2276:21272/151‑4 (MQ=60)
   GCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGgccc                                                                                                                                                     <  LH00941:114:22FVMWLT1:2:2129:44535:9311/151‑5 (MQ=60)
   GCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGgccc                                                                                                                                                     <  LH00941:114:22FVMWLT1:2:1144:15607:7405/151‑5 (MQ=60)
   GCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGgccc                                                                                                                                                     <  LH00941:114:22FVMWLT1:2:1149:30011:6252/151‑5 (MQ=60)
   GCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGgccc                                                                                                                                                     <  LH00941:114:22FVMWLT1:2:1154:9025:8782/151‑5 (MQ=60)
   GCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGgccc                                                                                                                                                     <  LH00941:114:22FVMWLT1:2:1154:9043:8782/151‑5 (MQ=60)
   GCTGTCACTGTGCAGGAAATGCAGGTCTGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTTCTTTTGTCATTTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGgccc                                                                                                                                                     <  LH00941:114:22FVMWLT1:2:2129:44553:9311/151‑5 (MQ=60)
        CACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGgccctttcg                                                                                                                                                <  LH00941:114:22FVMWLT1:2:1233:21709:5580/151‑10 (MQ=60)
        CACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCA                                                                                                                                                <  LH00941:114:22FVMWLT1:2:1168:48639:6717/151‑1 (MQ=60)
                   AAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTG                                                                                                                                     <  LH00941:114:22FVMWLT1:2:1270:9505:17237/151‑1 (MQ=60)
                   AAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTG                                                                                                                                     <  LH00941:114:22FVMWLT1:2:2133:27589:4139/151‑1 (MQ=60)
                        CAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGC                                                                                                                                <  LH00941:114:22FVMWLT1:2:1116:51117:20712/151‑1 (MQ=60)
                        CAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CAC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGC                                                                                                                                <  LH00941:114:22FVMWLT1:2:2266:51811:23866/151‑1 (MQ=60)
                        CAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CAC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGC                                                                                                                                <  LH00941:114:22FVMWLT1:2:2266:51829:23866/151‑1 (MQ=60)
                         AGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCA                                                                                                                               <  LH00941:114:22FVMWLT1:2:2235:6621:7277/151‑1 (MQ=60)
                           GTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCNAC                                                                                                                             <  LH00941:114:22FVMWLT1:2:1222:2840:1048/151‑1 (MQ=60)
                           GTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCNAC                                                                                                                             <  LH00941:114:22FVMWLT1:2:1222:2849:1064/151‑1 (MQ=60)
                           GTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAAC                                                                                                                             <  LH00941:114:22FVMWLT1:2:2139:27052:1384/151‑1 (MQ=60)
                           GTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAAC                                                                                                                             <  LH00941:114:22FVMWLT1:2:2170:22495:3418/151‑1 (MQ=60)
                           GTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAAC                                                                                                                             <  LH00941:114:22FVMWLT1:2:1267:19934:18358/151‑1 (MQ=60)
                           GTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAAC                                                                                                                             <  LH00941:114:22FVMWLT1:2:2166:45422:11296/151‑1 (MQ=60)
                           GTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAAC                                                                                                                             <  LH00941:114:22FVMWLT1:2:1246:32359:20215/151‑1 (MQ=60)
                             CGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAA                                                                                                                           <  LH00941:114:22FVMWLT1:2:2139:4208:11360/151‑1 (MQ=60)
                                CCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATNTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACC                                                                                                                        <  LH00941:114:22FVMWLT1:2:2217:15949:26412/151‑1 (MQ=60)
                                CCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACC                                                                                                                        <  LH00941:114:22FVMWLT1:2:2112:18131:28654/151‑1 (MQ=60)
                                CCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACC                                                                                                                        <  LH00941:114:22FVMWLT1:2:1248:32673:6412/151‑1 (MQ=60)
                                CCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACC                                                                                                                        <  LH00941:114:22FVMWLT1:2:1248:32664:6396/151‑1 (MQ=60)
                                CCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACC                                                                                                                        <  LH00941:114:22FVMWLT1:2:2248:24482:9391/151‑1 (MQ=60)
                                CCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACC                                                                                                                        <  LH00941:114:22FVMWLT1:2:2126:15431:4795/151‑1 (MQ=60)
                                CCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACC                                                                                                                        <  LH00941:114:22FVMWLT1:2:2167:30399:27421/151‑1 (MQ=60)
                                CCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACC                                                                                                                        <  LH00941:114:22FVMWLT1:2:1132:42408:20872/151‑1 (MQ=60)
                                CCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACC                                                                                                                        <  LH00941:114:22FVMWLT1:2:1143:40217:4587/151‑1 (MQ=60)
                                CCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACC                                                                                                                        <  LH00941:114:22FVMWLT1:2:1143:40208:4603/151‑1 (MQ=60)
                                CCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑AGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACC                                                                                                                        <  LH00941:114:22FVMWLT1:2:1154:4079:4795/151‑1 (MQ=60)
                                  TGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTG                                                                                                                      <  LH00941:114:22FVMWLT1:2:1155:12584:5756/151‑1 (MQ=60)
                                  TGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTG                                                                                                                      <  LH00941:114:22FVMWLT1:2:1248:18408:14659/151‑1 (MQ=60)
                                     GCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGG                                                                                                                   <  LH00941:114:22FVMWLT1:2:1219:16994:27389/151‑1 (MQ=60)
                                     GCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGG                                                                                                                   <  LH00941:114:22FVMWLT1:2:2216:36547:19110/151‑1 (MQ=60)
                                      CAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTTAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGC                                                                                                                  <  LH00941:114:22FVMWLT1:2:2121:4458:14803/151‑1 (MQ=60)
                                      CAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGC                                                                                                                  <  LH00941:114:22FVMWLT1:2:1103:42806:17493/151‑1 (MQ=60)
                                      CAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGC                                                                                                                  <  LH00941:114:22FVMWLT1:2:2166:3173:18246/151‑1 (MQ=60)
                                      CAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGC                                                                                                                  <  LH00941:114:22FVMWLT1:2:2213:39163:26749/151‑1 (MQ=60)
                                      CAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGC                                                                                                                  <  LH00941:114:22FVMWLT1:2:1142:51404:22585/151‑1 (MQ=60)
                                       AGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCT                                                                                                                 <  LH00941:114:22FVMWLT1:2:1207:18316:28622/151‑1 (MQ=60)
                                       AGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCT                                                                                                                 <  LH00941:114:22FVMWLT1:2:1207:18334:28622/151‑1 (MQ=60)
                                           ATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTA                                                                                                             <  LH00941:114:22FVMWLT1:2:1270:46587:11200/151‑1 (MQ=60)
                                           ATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTA                                                                                                             <  LH00941:114:22FVMWLT1:2:1270:46569:11200/151‑1 (MQ=60)
                                           ATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTA                                                                                                             <  LH00941:114:22FVMWLT1:2:1263:24575:9999/151‑1 (MQ=60)
                                             TAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATG                                                                                                           <  LH00941:114:22FVMWLT1:2:2115:16624:5067/151‑1 (MQ=60)
                                              AGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGC                                                                                                          <  LH00941:114:22FVMWLT1:2:1141:33801:7918/151‑1 (MQ=60)
                                              AGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGC                                                                                                          <  LH00941:114:22FVMWLT1:2:1242:4578:17605/151‑1 (MQ=60)
                                              AGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGC                                                                                                          <  LH00941:114:22FVMWLT1:2:2140:16985:12513/151‑1 (MQ=60)
                                              AGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGC                                                                                                          <  LH00941:114:22FVMWLT1:2:2140:16994:12529/151‑1 (MQ=60)
                                              AGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGC                                                                                                          <  LH00941:114:22FVMWLT1:2:2105:39274:14066/151‑1 (MQ=60)
                                              AGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGC                                                                                                          <  LH00941:114:22FVMWLT1:2:2140:17003:12513/151‑1 (MQ=60)
                                              AGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGC                                                                                                          <  LH00941:114:22FVMWLT1:2:2121:3320:19463/151‑1 (MQ=60)
                                                TCGGGCGGACATGAGTAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGGGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAG                                                                                                        <  LH00941:114:22FVMWLT1:2:1149:23373:12241/151‑1 (MQ=60)
                                                TCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAG                                                                                                        <  LH00941:114:22FVMWLT1:2:2218:22504:20023/151‑1 (MQ=60)
                                                TCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAG                                                                                                        <  LH00941:114:22FVMWLT1:2:1105:12223:23898/151‑1 (MQ=60)
                                                TCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAG                                                                                                        <  LH00941:114:22FVMWLT1:2:1154:30020:17605/151‑1 (MQ=60)
                                                TCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAG                                                                                                        <  LH00941:114:22FVMWLT1:2:1150:40227:13762/151‑1 (MQ=60)
                                                TCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAG                                                                                                        <  LH00941:114:22FVMWLT1:2:1227:46356:17205/151‑1 (MQ=60)
                                                 CGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGC                                                                                                       <  LH00941:114:22FVMWLT1:2:2123:31037:4379/151‑1 (MQ=60)
                                                 CGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGC                                                                                                       <  LH00941:114:22FVMWLT1:2:1116:20609:24555/151‑1 (MQ=60)
                                                 CGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGC                                                                                                       <  LH00941:114:22FVMWLT1:2:1116:20599:24571/151‑1 (MQ=60)
                                                  GGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCC                                                                                                      <  LH00941:114:22FVMWLT1:2:2231:11965:5964/151‑1 (MQ=60)
                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCTCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                     <  LH00941:114:22FVMWLT1:2:1157:42418:19607/151‑1 (MQ=60)
                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                     <  LH00941:114:22FVMWLT1:2:2146:32525:10191/151‑1 (MQ=60)
                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                     <  LH00941:114:22FVMWLT1:2:2215:17844:16052/151‑1 (MQ=60)
                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                     <  LH00941:114:22FVMWLT1:2:1124:13416:28782/151‑1 (MQ=60)
                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                     <  LH00941:114:22FVMWLT1:2:1132:38100:14467/151‑1 (MQ=60)
                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                     <  LH00941:114:22FVMWLT1:2:2250:26794:18102/151‑1 (MQ=60)
                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                     <  LH00941:114:22FVMWLT1:2:2161:5262:17765/151‑1 (MQ=60)
                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                     <  LH00941:114:22FVMWLT1:2:1110:13398:15876/151‑1 (MQ=60)
                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                     <  LH00941:114:22FVMWLT1:2:1106:38812:1544/151‑1 (MQ=60)
                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                     <  LH00941:114:22FVMWLT1:2:2268:6011:15315/151‑1 (MQ=60)
                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                     <  LH00941:114:22FVMWLT1:2:2207:10282:26108/151‑1 (MQ=60)
                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                     <  LH00941:114:22FVMWLT1:2:2207:9829:20007/151‑1 (MQ=60)
                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                     <  LH00941:114:22FVMWLT1:2:1219:44433:12113/151‑1 (MQ=60)
                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                     <  LH00941:114:22FVMWLT1:2:2215:17854:16036/151‑1 (MQ=60)
                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                     <  LH00941:114:22FVMWLT1:2:1230:27764:19239/151‑1 (MQ=60)
                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                     <  LH00941:114:22FVMWLT1:2:2243:45857:25948/151‑1 (MQ=60)
                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                     <  LH00941:114:22FVMWLT1:2:2109:48972:22313/151‑1 (MQ=60)
                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                     <  LH00941:114:22FVMWLT1:2:2109:22171:23034/151‑1 (MQ=60)
                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                     <  LH00941:114:22FVMWLT1:2:1262:18880:19671/151‑1 (MQ=60)
                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                     <  LH00941:114:22FVMWLT1:2:2102:44359:19127/151‑1 (MQ=60)
                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                     <  LH00941:114:22FVMWLT1:2:1250:37962:1833/151‑1 (MQ=60)
                                                       GACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTC                                                                                                 >  LH00941:114:22FVMWLT1:2:2258:27330:4523/1‑151 (MQ=60)
                                                                              TGTGTCGCAGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCTTTGTGTCGCGAAGGGTC‑‑‑CAC‑‑‑A‑‑‑‑TG‑‑‑GCGGCCCTGGAAATCTCCGTGTGTAGTCAACAAACTTGGGGCTGCTAGGCATCCCTTTCGCGACACAAGGCCCACAAAGGAC                                                                          <  LH00941:114:22FVMWLT1:2:1259:33977:22281/151‑1 (MQ=57)
                                                                                    GCCGACAGCATAGAAACACCGCTCGGGTGTGGGAGCGGCTTTGTGTCGCGAAAGGGC‑‑‑CGCGGAG‑‑‑‑TG‑‑‑GCC‑‑‑CTGGCAGTTTCCGTGTGTGTGCAACAAACTTGGGGTTGTTGTGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCA                                                                    <  LH00941:114:22FVMWLT1:2:1253:16569:28254/151‑1 (MQ=60)
                                                                                              TAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCctgtctcttatacacatctccgagcccacgagactcgtgcattcatctggtggggg                                                          >  LH00941:114:22FVMWLT1:2:2214:23604:7485/1‑95 (MQ=60)
                                                                                               AGCAACCCCCCTCAGGTGTGGGAGCGACTTTGTGTCGCGAAAGGGCAAAGGG‑‑‑G‑‑‑‑TG‑‑‑GCC‑‑‑CCGGCAGTTTCCGCGTGTATAAAACAAACCTGGGTTTGCTATGAGGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC                                                         <  LH00941:114:22FVMWLT1:2:2140:21478:23050/151‑1 (MQ=45)
                                                                                                    CACCGCTCAGGTGTGGGGGGGGCCTTGTGTCGCGAAGGGGC‑‑‑AGA‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCACTTTCCGTGTGTAGGCAACAAACCGGGGGTTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAA                                                    <  LH00941:114:22FVMWLT1:2:2225:15746:9151/151‑1 (MQ=60)
                                                                                                    CACCGCTCAGGTGTGGGAGTGGCTTTGTGTCGAGAAGGGGC‑‑‑CAC‑‑‑A‑‑‑‑GA‑‑‑GCGGCCCCGACATTTTCCGCGTGTATGCAACAAACTTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAA                                                    <  LH00941:114:22FVMWLT1:2:2225:15718:9167/151‑1 (MQ=60)
                                                                                                     cccgtTCAGGTGTGGGAGTGGCTTTGTGTCGAGAAAGTGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGTCCCCGAAAATTTCCGCGTGTACAAAACAACCTTGGGGTTACTATGCACCCCTTTCGCAACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAAC                                                   <  LH00941:114:22FVMWLT1:2:1118:8211:28734/146‑1 (MQ=51)
                                                                                                     ACCTCTCGGGTGGGGGAGCGGCTTTGTGTCGAGAAGGGGC‑‑‑CGC‑‑‑ATGG‑TG‑‑‑GCC‑‑‑CTGGCAGTTTCCGCGTGTGTGCAACAACCTTGGGGCTGCTATGCAGCCCTTTTGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAAC                                                   <  LH00941:114:22FVMWLT1:2:1248:33265:28478/151‑1 (MQ=60)
                                                                                                     ACCGCTAAGGTGGGGGGGCGGCTTTGTGTTGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑GA‑‑‑GCGTCCCCGGCAATTTCCGAGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCTTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAAC                                                   <  LH00941:114:22FVMWLT1:2:1248:33256:28494/151‑1 (MQ=60)
                                                                                                          TCGGGTGTGGGAGTGGCCTTGTGTCGTGAAGGGGC‑‑‑CGCGGGG‑‑‑‑TG‑‑‑GCC‑‑‑CTGGCATTTTCCGGGTGTGTGCAACAAACTTGGGGTTGCGATGCACCCTTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCC                                              <  LH00941:114:22FVMWLT1:2:1264:23456:3098/151‑1 (MQ=53)
                                                                                                          TCAGGTGTGGGGGTGACCTTGTTTCGCGAAAGGTC‑‑‑CGA‑‑‑A‑‑‑‑TA‑‑‑GCGACCCCGGAAATTTCTGCGGGTATGCAAAAAACTTGGGGTTGCTAGGAAGCCTTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCC                                              <  LH00941:114:22FVMWLT1:2:1134:4504:11296/151‑1 (MQ=58)
                                                                                                          TCAGGTGTGGGAGTGGCTTGGTGTCGCGAAGGGGC‑‑‑CG‑‑‑‑AGGAGTG‑‑‑GCC‑‑‑CCGGCAGTTTCCGTGTGTGTGCAACAAACTTGGGGTTGCTATGCATCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCC                                              <  LH00941:114:22FVMWLT1:2:1169:37573:4331/151‑1 (MQ=60)
                                                                                                               TGTGGGAGTGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACTTGGGGCTGCTATGCAGCCCTTTCTCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGC                                         <  LH00941:114:22FVMWLT1:2:2161:32978:13026/151‑1 (MQ=60)
                                                                                                               TGTGGGAGCGGTCTTGTGTCGAGAAGGGGC‑‑‑CGA‑‑‑AGAG‑TG‑‑‑GCC‑‑‑CCGGCAATTTCCGCGTGGATGAAACAAACTTGGGGCTGCTATGCACCCTTTTCGCAACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGC                                         <  LH00941:114:22FVMWLT1:2:1238:8978:6620/151‑1 (MQ=60)
                                                                                                               TGTGGGAGCGGCTTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TAGTGGCC‑‑‑CCGGCAATTTCCGCGTGTATGCAACAAACTTTGGGCTGCTATGAAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGC                                         <  LH00941:114:22FVMWLT1:2:2161:32988:13042/151‑1 (MQ=60)
                                                                                                                          CCTTTTGTCGCGAAAGGGC‑‑‑CGC‑‑‑ATGG‑TG‑‑‑GCC‑‑‑CCGGCAGTTTCCGCGTGGATGCAACAAACTTGGGGCTGCTATGCATCCCTTTCTCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGC                              <  LH00941:114:22FVMWLT1:2:2251:42020:20552/151‑1 (MQ=60)
                                                                                                                               TGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑GA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTAGGAAACAAACTTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGC                         <  LH00941:114:22FVMWLT1:2:2269:15644:14259/151‑1 (MQ=60)
                                                                                                                               TGTAGAGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCTGGCATTTTCCGTGTGTAGGCAACAAACTTGGGTCTGCTATGCATCCCTTTCGCAACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGC                         <  LH00941:114:22FVMWLT1:2:2108:29641:24539/151‑1 (MQ=60)
                                                                                                                                    CGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCA                    >  LH00941:114:22FVMWLT1:2:1264:13915:9823/1‑151 (MQ=60)
                                                                                                                                      AAAGGGC‑‑‑CGC‑‑‑AGAG‑TG‑‑‑GCC‑‑‑CCGGCAATTTCCGCGTGGGGGAAACAAACTTGGGGCTGCTATGCAGCCTTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGA                  <  LH00941:114:22FVMWLT1:2:1157:38701:19062/151‑1 (MQ=60)
                                                                                                                                        GGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATC                >  LH00941:114:22FVMWLT1:2:1139:8599:16340/1‑151 (MQ=60)
                                                                                                                                         GGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCA               >  LH00941:114:22FVMWLT1:2:1211:21062:20055/1‑151 (MQ=60)
                                                                                                                                         GGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCA               >  LH00941:114:22FVMWLT1:2:2257:37490:25868/1‑151 (MQ=60)
                                                                                                                                         GGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCAAAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCA               >  LH00941:114:22FVMWLT1:2:2149:19527:9135/1‑151 (MQ=60)
                                                                                                                                            C‑‑‑CGC‑‑‑G‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGTGTGTATGCAAAAACCCTGGGTCTGCTGTACGTCCTTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCC            <  LH00941:114:22FVMWLT1:2:1221:41724:19078/151‑1 (MQ=60)
                                                                                                                                            C‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCC            >  LH00941:114:22FVMWLT1:2:1110:2442:7693/1‑151 (MQ=60)
                                                                                                                                            C‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCC            >  LH00941:114:22FVMWLT1:2:1110:2433:7709/1‑151 (MQ=60)
                                                                                                                                                 GC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAG          >  LH00941:114:22FVMWLT1:2:2229:5854:7549/1‑151 (MQ=60)
                                                                                                                                                 GC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAG          >  LH00941:114:22FVMWLT1:2:1267:5410:21961/1‑151 (MQ=60)
                                                                                                                                                 GC‑‑‑A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAG          >  LH00941:114:22FVMWLT1:2:2213:19148:6524/1‑151 (MQ=60)
                                                                                                                                                      A‑‑‑‑TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCTCGCAGCTGAGCCGCCAGATCATCCAGCA        >  LH00941:114:22FVMWLT1:2:1105:21025:13330/1‑151 (MQ=60)
                                                                                                                                                           TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCAC       >  LH00941:114:22FVMWLT1:2:2113:46180:4379/1‑151 (MQ=60)
                                                                                                                                                           TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCAC       >  LH00941:114:22FVMWLT1:2:2114:8368:17093/1‑151 (MQ=60)
                                                                                                                                                           TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCAAAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCAGCCAGATCATCAAGCAC       >  LH00941:114:22FVMWLT1:2:2238:34985:17077/1‑151 (MQ=60)
                                                                                                                                                           TA‑‑‑GCGGCCCCGGCAATTTCCGCGTGCGTGCAAAAAACCGGGGGTTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCAC       <  LH00941:114:22FVMWLT1:2:1113:51376:14883/151‑1 (MQ=60)
                                                                                                                                                                GCC‑‑‑CCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCTTGC  >  LH00941:114:22FVMWLT1:2:1131:18279:11168/1‑151 (MQ=60)
                                                                                                                                                                  |                                                                                                                                                       
TACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGC‑‑‑CGC‑‑‑A‑‑‑‑TA‑‑‑GCG‑‑‑CCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCTTGC  >  AE015451_phaM1/5555459‑5555756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑