Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F11 I4 R1	74	758.2	33768536	99.3%	33532156	136.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451_phaM1	5,555,608	+GCC	intergenic (‑84/+87)	PP_4887 CDS (conserved protein of unknown function) ← / ← PP_4888 CDS (putative methyl‑accepting chemotaxis transducer)	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	5,555,608	1	.	G	94.5%	650.1 / 38.6	230	intergenic (‑84/+87)	PP_4887 CDS (conserved protein of unknown function)/PP_4888 CDS (putative methyl‑accepting chemotaxis transducer)	–/–
Reads supporting (aligned to +/- strand): ref base . (0/12); new base G (8/200); total (9/221)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	AE015451_phaM1	5,555,608	2	.	C	94.3%	756.5 / 40.0	230	intergenic (‑84/+87)	PP_4887 CDS (conserved protein of unknown function)/PP_4888 CDS (putative methyl‑accepting chemotaxis transducer)	–/–
Reads supporting (aligned to +/- strand): ref base . (1/12); new base C (7/209); total (9/221)
Fisher's exact test for biased strand distribution p-value = 3.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	AE015451_phaM1	5,555,608	3	.	C	87.8%	719.2 / 103.4	230	intergenic (‑84/+87)	PP_4887 CDS (conserved protein of unknown function)/PP_4888 CDS (putative methyl‑accepting chemotaxis transducer)	–/–
Reads supporting (aligned to +/- strand): ref base . (1/27); new base C (8/194); total (9/221)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 236 total aligned reads displayed.

TGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑G‑‑‑CC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCT  >  AE015451_phaM1/5555472‑5555729

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              gTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTc                                                                                                                <  1:10261331/137‑1 (MQ=38)

                cGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCcg                                                                                                              <  1:6985356/137‑1 (MQ=38)

                   ccTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCgt                                                                                                           <  1:3636947/137‑1 (MQ=38)

                   ccTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCgt                                                                                                           <  1:248005/137‑1 (MQ=38)

                   ccTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCgt                                                                                                           <  2:2585074/137‑1 (MQ=38)

                   ccTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCgt                                                                                                           <  1:645655/137‑1 (MQ=38)

                   ccTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCgt                                                                                                           <  1:12776866/137‑1 (MQ=38)

                   ccTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCgt                                                                                                           <  1:12450745/137‑1 (MQ=38)

                   ccTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCgt                                                                                                           <  2:8427019/137‑1 (MQ=38)

                   ccTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCgt                                                                                                           <  1:11201663/137‑1 (MQ=38)

                        gCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATg                                                                                                      <  2:2655986/137‑1 (MQ=38)

                         cAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TAGTGGC‑C‑‑‑CC‑GGCAATTTCCGCGTGTATGc                                                                                                     <  2:10163652/137‑1 (MQ=38)

                         cAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TAGTGGC‑C‑‑‑CC‑GGCAATTTCCGCGTGTATGc                                                                                                     <  2:6259154/137‑1 (MQ=38)

                         cAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGc                                                                                                     <  2:11400432/137‑1 (MQ=38)

                         cAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGc                                                                                                     <  1:6262497/137‑1 (MQ=38)

                         cAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGc                                                                                                     <  2:14634694/137‑1 (MQ=38)

                         cAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGc                                                                                                     <  2:1558118/137‑1 (MQ=38)

                         cAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGc                                                                                                     <  1:9187689/137‑1 (MQ=38)

                         cAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGc                                                                                                     <  2:3574597/137‑1 (MQ=38)

                         cAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGc                                                                                                     <  2:4802917/137‑1 (MQ=38)

                         cAGCGATTAGGCGGGCGGACATGAGCACCTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGc                                                                                                     <  2:14394423/137‑1 (MQ=37)

                            cGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGcaac                                                                                                  <  1:7700270/137‑1 (MQ=38)

                              aTTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGcaacaa                                                                                                <  1:14640707/137‑1 (MQ=38)

                              aTTAGTCGGGCGGACAGGAGCAACTCCTTTTGTCATGTGTCGCCGATAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGcaacaa                                                                                                <  1:4769595/137‑1 (MQ=37)

                               ttAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACaaa                                                                                               <  2:16501069/137‑1 (MQ=38)

                                 aGTCGGGCGGACATGAGCAACTCCTTTTGTCGTGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAAcc                                                                                             <  1:292897/137‑1 (MQ=38)

                                 aGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCTCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAAcc                                                                                             <  1:6820123/137‑1 (MQ=38)

                                 aGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAAcc                                                                                             <  2:12823130/137‑1 (MQ=38)

                                 aGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAAcc                                                                                             <  1:14670843/137‑1 (MQ=38)

                                   tCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTg                                                                                           <  2:4480582/137‑1 (MQ=38)

                                   tCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTg                                                                                           <  2:7873825/137‑1 (MQ=38)

                                   tCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTg                                                                                           <  1:10765142/137‑1 (MQ=38)

                                   tCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTg                                                                                           <  1:2370994/137‑1 (MQ=38)

                                    cGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgg                                                                                          <  2:5028335/137‑1 (MQ=38)

                                    cGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgg                                                                                          <  2:8578557/137‑1 (MQ=38)

                                      ggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                        <  2:1113326/137‑1 (MQ=38)

                                      ggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                        <  2:13684790/137‑1 (MQ=38)

                                      ggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                        <  1:1574077/137‑1 (MQ=38)

                                      ggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                        <  1:16108474/137‑1 (MQ=38)

                                      ggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                        <  1:13487623/137‑1 (MQ=38)

                                      ggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                        <  1:4547100/137‑1 (MQ=38)

                                      ggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                        <  1:16737681/137‑1 (MQ=38)

                                      ggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                        <  1:7811333/137‑1 (MQ=38)

                                      ggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                        <  1:16842173/137‑1 (MQ=38)

                                      ggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                        <  1:3742633/137‑1 (MQ=38)

                                      ggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                        <  1:9924712/137‑1 (MQ=38)

                                      ggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                        <  1:8539223/137‑1 (MQ=38)

                                      ggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                        <  1:3442406/137‑1 (MQ=38)

                                      ggCGGACATGAGCAACTCCTTGGGTCATGTGTCGCCGACAGCATAGCAACACCGCTCGGGTGTGGGGGCGGCTTTGTGTCGCGAAAGTGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTATATGCAACAACCCttttc                                                                                        <  2:10057073/137‑5 (MQ=255)

                                            cATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTa                                                                                  <  1:12112932/137‑1 (MQ=38)

                                              tGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATg                                                                                <  2:9212202/137‑1 (MQ=38)

                                                      tCCTTTTGTCTTTTGTCGCCGACAGCATAGCAACACCACTCGGGTGTGGGAGTGGCCTTGTTTCGCGAAAGGGCCACA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCACGTGTATGCAACAAACCTGGGGCGGCTATGCAGCCCtt                                                                        <  2:13163343/137‑1 (MQ=255)

                                                      tCCTTTTGTCATGTTTCGCCGACAGCATAGCACCACCGCTCATGTGTGGGAGCGGCCTTGTGTCGAGAAAGGTCCACA‑‑‑TA‑‑‑CC‑GACCCC‑GGCAATTTCCGCGTGTAGTCAACAAACCCGGGTTTCCTATGCAGCCCtt                                                                        <  2:1688712/137‑1 (MQ=255)

                                                      tCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGNGTGTATGCAACAAACCTGGGGCTGCTATGCAGcccca                                                                        <  1:16290945/137‑3 (MQ=38)

                                                      tCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCtt                                                                        <  2:7839405/137‑1 (MQ=38)

                                                      tCCTTTTGCCATGTGTCGCCGACAGCGTAACAACACCTCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACTTGGGGCTGTTATGCAGCCCtt                                                                        <  2:10314122/137‑1 (MQ=25)

                                                             ttCATGTGTCGCCGACAGCATAGCAACACCACCCAGTTGTGGGAGCGGCCTTGTTTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GT‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGTCTGCTATGCATCCCTTTCGCGac                                                                 <  2:11573290/136‑1 (MQ=255)

                                                             ttCATGTGTCGCCGACAGCATAGAAACACCTCCCAGGTGTGGGAGCGGCCTTGTGTCGAGAAAGGGCCGCA‑‑‑TA‑‑‑TC‑GGCCCC‑GACAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGac                                                                 <  2:3261764/136‑1 (MQ=37)

                                                             gTCATGTGTCGCTGACAAAATAGAAACACCGCTAAGGTGTGGGAGCGGCCTTGTGCCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GCTGCC‑CC‑GGCAATTTCCGCGTGTGTGAAACAAACCTGGGGCTGCGGTGCAGCCCTTCCGCGac                                                                 <  2:11964227/137‑1 (MQ=25)

                                                              tCATGTGTCACCGACAACATAGCAACCCCTCTCAGGTGTGGGAGCGGCCTTGTGTCGCAAAAGGGCCGCA‑‑‑TAGTGGC‑C‑‑‑CC‑GGCAATTTCCGTGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCTTTTCGCGaca                                                                <  2:2922573/137‑1 (MQ=25)

                                                               cgtgtgTCGCCGACAGCAGAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTTTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGacac                                                               <  2:16237769/135‑1 (MQ=38)

                                                               cATTTGTCGCCGACAGCATAGCAACACCGTTCAGGTTTGGGGGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTTGATGCAAAAAACCTGGGGCTGCTATGCAGCCCTTTCGCGacac                                                               <  2:9545257/137‑1 (MQ=25)

                                                               cATGTTTCGCCAAAAGCAGAGCACCACCGCCCAGGTGTGGGAGCGGCTTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGacac                                                               <  2:5578877/137‑1 (MQ=25)

                                                                 tttttCGCCGACAGCGTAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGAA‑‑‑TA‑‑‑GCTGCC‑CC‑GGAAATTTCCGCGTGTAGGAAACAAACCTGGGTCTGCTATGCAGCCCTTTCGCGACACaa                                                             <  2:4296894/133‑1 (MQ=25)

                                                                 tttgtCGCTGAAAGCGTGGCAACCCCTCTCGGGTGTGGGGGGGGCTTTGTTTTGTGAAGGGGCCGCG‑‑‑TG‑‑‑GT‑GGC‑CCTGGCAGTTTCTGTGTGGGTGAAAAAAACTTGGGGTTGTTGTGCAGCCCTTTCGCGACACaa                                                             <  2:4996810/135‑1 (MQ=255)

                                                                 tgttttgCCGACAGCATAGCAACACCGTTCAGGTGTGGGAGCGGCCTTTTGTCGCGAAAGGGCCACA‑‑‑TA‑‑‑TC‑GGCCCC‑GGAAATTTCCGCGTGCATGCAACAAACCTTGGGCTGCTGGGCAGCCCTTTCGCGACACaa                                                             <  2:16587139/131‑1 (MQ=25)

                                                                 tgtgTCGCCGACAGCATAGCAACACCTCTCAGTTGTGGGAGCGGCTTTGTGTCGCGAAAGGGCCGCA‑‑‑TAGTGGC‑C‑‑‑CC‑GGCAATTTCCGCGTGTATGCAAAAAACCTGGGGCTGCTATGCAGCCTTTTCGCGACACaa                                                             <  2:2095948/137‑1 (MQ=25)

                                                                 tgtgTCACCGACAGCGTGGCAACACCTCCCGGGTGTGGGAGTGGCCTTGTGTCGCGAAGGGGCCCCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGAAATTTCCGTGTGTAGGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACaa                                                             <  2:8648697/137‑1 (MQ=21)

                                                                      cGCCGACAGCATAGCAACCCCGCTCAGGTGTGGGAGCGGCTTGGTGTCGAGAAGGGGCCGCAGGGTG‑‑‑GC‑C‑‑‑CC‑GGAAGTTTCCGCGTGGAGGAAAAAAACCTGGGGTGGTGATGAAGCCTTTTCGCGACACAAGGccc                                                        <  2:10875886/137‑1 (MQ=255)

                                                                      cGCCGACAGCATAACAACCCCCCTCAGGTGTGGGAGCGGCCTTGTGTCGCAAAAGGTCCACA‑‑‑TA‑‑‑GC‑GTC‑CCAGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCGGCGATGCAGCCCTTTCGCGACACAAGGccc                                                        <  2:2635192/137‑1 (MQ=37)

                                                                      cGCCGACAGCAGAGCAACACTGCTCAGGTGGGGGAGGGGCCTTGTGTAGAGAAGGGTCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCATTTTCCGTGTGTATGAAACAACCCTGGGGCTGCCGTGCAGCCCTTTCGCGACACAAGGccc                                                        <  2:14909029/137‑1 (MQ=255)

                                                                           acaacaGAGCAACACCGCCAAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCTGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCACCAACCCTGGGGCGGCTATGCAGCCCTTTCGCGACACAAGGCCCACaaa                                                   <  2:3890887/137‑1 (MQ=25)

                                                                           aCAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCGTGTGTCTCGAAAGGGCCGCA‑‑‑TG‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGTGATGCAGCCCTTTCGCGACACAAGGCCCACaaa                                                   <  2:14715558/137‑1 (MQ=37)

                                                                                agaacAACACTGCTCGGGTGTGGGAGGGGCTTGGTGTCGAGAAGGGGCCGCA‑‑‑GAGGGGC‑C‑‑‑CC‑GGAAGTTTCCGCGGGGGTGAAACAAACCTGGGGCGGCGAGGAAGCCCTTTCGAGACACAAGGCCCACAAAGGAcc                                              <  2:186842/133‑1 (MQ=255)

                                                                                aTAGCAACACCGCTCAGGTGTGGGAGCGGCTTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGC‑CCTGGAAATTTCCGCGTGGATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGAAACAAGGCCCACAAAGGAcc                                              <  2:10482564/137‑1 (MQ=37)

                                                                                aTAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTTTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCTGCGTGTATGCAACAAACCTGGGGTTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGAcc                                              <  2:16339241/137‑1 (MQ=38)

                                                                                  aacAACACCGTTCGGGTGGGGGAGAGGCTTGGTGTTGTGAAGGGGCGGAA‑‑‑TA‑‑‑GT‑GGC‑CCTGGAAGTTTCTGCGGGTATGAAACAAACCTGGGGTTGCTGGGCAGCCTTTTCGAGAAACAAGGCCCACAAAGGACcgc                                            <  2:894501/135‑1 (MQ=255)

                                                                                  aGCAACACCGCTCAGGGTTGGGAGCGGCCTTGTTTCTCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGAGTGTATGCAGCAACCTTGGGTCGGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACcgc                                            <  2:14661377/137‑1 (MQ=25)

                                                                                        acCGCTCAGGTGTGGGATCGGCCTTGTGTCGCGAAAGGGACACA‑‑‑TAGAGGC‑C‑‑‑CC‑GGCAGTTTCCGTGTGTAGGCAACAAACCTGTGTTTGCGATGCAGCCCTTTCGAGAAACAAGGCCCACAAAGGACCGCGCAgga                                      <  2:15178972/137‑2 (MQ=19)

                                                                                        acCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGAA‑‑‑TA‑‑‑GC‑GGC‑CCTGGCAATTTCCGCGTGTGTGCAACAAACCTGGGGCTGCGATGCAGCCTTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGc                                      <  2:12502573/137‑1 (MQ=37)

                                                                                        acCGCTCAGGTGTGGGAGCGGCCTTGTGTCCCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GCCCCC‑GGCAATTTCCGCGTGGATACAACAAACCTGGGGCTGCTATGCAGCCCTTTCTCGACACAAGGCCCACAAAGGACCGCGCAGGc                                      <  2:12301097/137‑1 (MQ=37)

                                                                                             tcgggTGGGGGGGCGGCCTTGTGTCGAGAAAGGGCCGCG‑‑‑GG‑‑‑GG‑GGC‑CCTGGCACTTTCTGTGTGTATGAAACAAACCTGGGGTTGCTAGGCAGCCTTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAg                                 <  2:251889/134‑1 (MQ=255)

                                                                                             tCAGGTGTGGGAGCGGCCTTTTGTCGCGAAAGGTCCGCA‑‑‑TATCGGC‑C‑‑‑CC‑GTCAATTTCCGCGTGTATGCAACAAGCCTGGTGCTGCTATGCAGCCTTTTCGTGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAg                                 <  2:15723129/137‑1 (MQ=255)

                                                                                             tCAGGTGTGGGAGCGGCCTTGTGTCTCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCGACTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAg                                 <  2:7014349/137‑1 (MQ=37)

                                                                                             tCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GACAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAg                                 <  2:16317385/137‑1 (MQ=38)

                                                                                             ccAGGTGTGGGAGCGGCTTTGTTTCGAGAAAGGGCCACA‑‑‑TATCGGC‑C‑‑‑CC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCGATGCAGCCTTTTCTCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAg                                 <  2:15422532/136‑1 (MQ=255)

                                                                                                  tgtgGGAGCGGCTTTTTGTCGCGAAGGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAGTTTCCGTGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCg                            <  2:5438768/137‑1 (MQ=37)

                                                                                                  tgtgGGAGCGGCCTTGTTTCGCGAAAGGTCCGCA‑‑‑CA‑‑‑GC‑GCCCCC‑GGCAATTCCCGTGTGGATGCAACAAACCTGGGGCTGCTGTGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCg                            <  2:4832861/137‑1 (MQ=25)

                                                                                                  tgtgGGAGCGGCCTTGTTTCGCGAAAGGGCTGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCg                            <  2:8285315/137‑1 (MQ=38)

                                                                                                  tgtgGGAGCGGCCTTGTTTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGTGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCg                            <  2:10720031/137‑1 (MQ=37)

                                                                                                  tgtgGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCTGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCTCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCg                            <  2:4078835/137‑1 (MQ=37)

                                                                                                  tgggggAGTGGCTTGGTGTCGAGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGAAGTTTCCGTGTGTATGAAAAAAACCTGGGGCTGCTAGGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCg                            <  1:5869540/134‑1 (MQ=25)

                                                                                                   gtgGGAGTGGCCTTGGGTCGCGAAAGGGCCGCA‑‑‑GA‑‑‑GC‑GGCCCC‑GGCAGTTTCCGCGTGTAGGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCgg                           <  2:7614104/137‑1 (MQ=37)

                                                                                                   gtgGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGGAGACAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCgg                           <  2:3318315/137‑1 (MQ=25)

                                                                                                   gggggAGCGGCCTGGTGTCGAGAAAGGGCCGAA‑‑‑GA‑‑‑GC‑GGCCCC‑GGCAATTTCCGGGGGTGTGCAAAAAACCTGGGGTTGCGATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCgg                           <  2:933285/135‑1 (MQ=21)

                                                                                                        aGCGGCTTGGTGTCGAGAAGGGGCTGCG‑‑‑TG‑‑‑GC‑GGC‑CCTGGCAGTTTCGGTGTGTGTGCAACAAACTTGGGTCTGCTATGCAACCTTTTCGAGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTg                      <  1:4894323/137‑1 (MQ=255)

                                                                                                                          aaGGGCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAAGTTCCGCGTGTATGCAAAAAACCTGGGGCTGCTATGTAGCCCTTTCGCGAAACAAAGCCCACAAAGGAACGCGCAGGCTTCAGGCCCGGAACTGGGCCAGGCTGGCGCGCAg    >  2:7896785/1‑137 (MQ=255)

                                                                                                                          aaGGGCCGCA‑‑‑TA‑‑‑GC‑GGACCC‑GGCAAGTTCCGCGTGTCTGCAACAAACCTGGGGTTGCTATGCAGCCCCTTCGCGACACAAAGCCCAAAAAGGACCGCGCAGGATTCAGGCCCGGAACTGGCCCAGGGTGGAGCGTAg    >  2:13558190/1‑137 (MQ=255)

                                                                                                                          aaGGGCCACA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGTGTGTATGCAAAAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAg    <  2:3094175/137‑1 (MQ=38)

                                                                                                                            gggCCGCA‑‑‑TA‑‑‑GC‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCt  >  2:10231799/1‑137 (MQ=255)

|||

TGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑GC‑G‑‑‑CC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCT  >  AE015451_phaM1/5555472‑5555729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CG‑‑‑CCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCAC  >  AE015451_phaM1/5555459‑5555751

TACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CG‑‑‑G                                                                                                                                                <  LH00941:114:22FVMWLT1:2:2169:31934:11857/151‑1 (MQ=60)

 ACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CG‑‑‑GC                                                                                                                                               <  LH00941:114:22FVMWLT1:2:2253:50960:15027/151‑1 (MQ=60)

   GCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGgccc                                                                                                                                                <  LH00941:114:22FVMWLT1:2:2257:37573:13106/151‑5 (MQ=60)

   GCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGgccc                                                                                                                                                <  LH00941:114:22FVMWLT1:2:2103:13250:29038/151‑5 (MQ=60)

   GCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGgccc                                                                                                                                                <  LH00941:114:22FVMWLT1:2:1210:47179:20552/151‑5 (MQ=60)

            GTGCAGGAAATTCGGGTCGACCTGGGCAGCGATGAGTCGGGCGGACATGAGCAACTCTTTTTGTCATGTGTCGCCGACAGCAGAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGgccctttcgcgac                                                                                                                                       <  LH00941:114:22FVMWLT1:2:1258:5447:7165/151‑14 (MQ=60)

             TGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTC                                                                                                                                      <  LH00941:114:22FVMWLT1:2:1259:14978:1673/151‑1 (MQ=60)

             TGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTC                                                                                                                                      <  LH00941:114:22FVMWLT1:2:2157:30760:24106/151‑1 (MQ=60)

                  GAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGT                                                                                                                                 <  LH00941:114:22FVMWLT1:2:1103:12492:14243/151‑1 (MQ=60)

                   AAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTG                                                                                                                                <  LH00941:114:22FVMWLT1:2:2127:4624:2761/151‑1 (MQ=60)

                       TCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATG                                                                                                                            <  LH00941:114:22FVMWLT1:2:2168:1961:18454/151‑1 (MQ=60)

                           GTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAAC                                                                                                                        <  LH00941:114:22FVMWLT1:2:2131:41262:19719/151‑1 (MQ=60)

                             CGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAA                                                                                                                      <  LH00941:114:22FVMWLT1:2:1121:12112:6348/151‑1 (MQ=60)

                             CGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAA                                                                                                                      <  LH00941:114:22FVMWLT1:2:1247:21052:5692/151‑1 (MQ=60)

                                CCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACC                                                                                                                   <  LH00941:114:22FVMWLT1:2:1105:49083:5564/151‑1 (MQ=60)

                                CCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACC                                                                                                                   <  LH00941:114:22FVMWLT1:2:2168:29965:4923/151‑1 (MQ=60)

                                CCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACC                                                                                                                   <  LH00941:114:22FVMWLT1:2:2203:7934:16340/151‑1 (MQ=60)

                                CCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACC                                                                                                                   <  LH00941:114:22FVMWLT1:2:2168:29955:4907/151‑1 (MQ=60)

                                CCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACC                                                                                                                   <  LH00941:114:22FVMWLT1:2:1161:34347:21288/151‑1 (MQ=60)

                                CCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACC                                                                                                                   <  LH00941:114:22FVMWLT1:2:1105:49074:5548/151‑1 (MQ=60)

                                CCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACC                                                                                                                   <  LH00941:114:22FVMWLT1:2:1106:19767:26845/151‑1 (MQ=60)

                                CCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACC                                                                                                                   <  LH00941:114:22FVMWLT1:2:2147:46661:11873/151‑1 (MQ=60)

                                CCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACC                                                                                                                   <  LH00941:114:22FVMWLT1:2:1111:18639:24315/151‑1 (MQ=60)

                                CCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACC                                                                                                                   <  LH00941:114:22FVMWLT1:2:1112:41641:24187/151‑1 (MQ=60)

                                     GCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGG                                                                                                              <  LH00941:114:22FVMWLT1:2:2263:1601:13858/151‑1 (MQ=60)

                                      CAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGC                                                                                                             <  LH00941:114:22FVMWLT1:2:1235:1379:7421/151‑1 (MQ=60)

                                      CAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGC                                                                                                             <  LH00941:114:22FVMWLT1:2:1235:1388:7405/151‑1 (MQ=60)

                                      CAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGC                                                                                                             <  LH00941:114:22FVMWLT1:2:1220:11216:8670/151‑1 (MQ=60)

                                      CAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGC                                                                                                             <  LH00941:114:22FVMWLT1:2:1235:1398:7421/151‑1 (MQ=60)

                                      CAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGC                                                                                                             <  LH00941:114:22FVMWLT1:2:2113:24122:21288/151‑1 (MQ=60)

                                      CAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGC                                                                                                             <  LH00941:114:22FVMWLT1:2:1158:35770:3418/151‑1 (MQ=60)

                                      CAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGC                                                                                                             <  LH00941:114:22FVMWLT1:2:1146:43980:19527/151‑1 (MQ=60)

                                      CAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGC                                                                                                             <  LH00941:114:22FVMWLT1:2:2107:11170:4779/151‑1 (MQ=60)

                                      CAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGC                                                                                                             <  LH00941:114:22FVMWLT1:2:1118:28559:10111/151‑1 (MQ=60)

                                         CGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGC                                                                                                          <  LH00941:114:22FVMWLT1:2:1258:47262:28894/151‑1 (MQ=60)

                                         CGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTAGGCAACAAACCTGGGGCTGC                                                                                                          <  LH00941:114:22FVMWLT1:2:1130:51884:16596/151‑1 (MQ=60)

                                           ATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTA                                                                                                        <  LH00941:114:22FVMWLT1:2:1241:30575:23498/151‑1 (MQ=60)

                                           ATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTA                                                                                                        <  LH00941:114:22FVMWLT1:2:2234:26054:5964/151‑1 (MQ=60)

                                           ATTAGTCGGGCGGACAGGAGCAACTCCTTTTGTCATGTGTCGCCGATAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTA                                                                                                        <  LH00941:114:22FVMWLT1:2:1210:47197:17093/151‑1 (MQ=60)

                                              AGTCGGGCGGACATGAGCAACTCCTTTTGTCGTGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGC                                                                                                     <  LH00941:114:22FVMWLT1:2:1105:19888:27245/151‑1 (MQ=60)

                                              AGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCTCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGC                                                                                                     <  LH00941:114:22FVMWLT1:2:1244:11946:13362/151‑1 (MQ=60)

                                              AGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCTCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGC                                                                                                     <  LH00941:114:22FVMWLT1:2:1244:11937:13378/151‑1 (MQ=60)

                                              AGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGC                                                                                                     <  LH00941:114:22FVMWLT1:2:1165:36492:25740/151‑1 (MQ=60)

                                              AGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGC                                                                                                     <  LH00941:114:22FVMWLT1:2:1163:48020:2153/151‑1 (MQ=60)

                                              AGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGC                                                                                                     <  LH00941:114:22FVMWLT1:2:2234:10337:20888/151‑1 (MQ=60)

                                                TCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCTGACAGCACAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAG                                                                                                   <  LH00941:114:22FVMWLT1:2:1165:47096:18518/151‑1 (MQ=60)

                                                TCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAG                                                                                                   <  LH00941:114:22FVMWLT1:2:2140:28837:3866/151‑1 (MQ=60)

                                                TCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAG                                                                                                   <  LH00941:114:22FVMWLT1:2:2144:12880:6396/151‑1 (MQ=60)

                                                TCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAG                                                                                                   <  LH00941:114:22FVMWLT1:2:1140:44988:17141/151‑1 (MQ=60)

                                                  GGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCC                                                                                                 <  LH00941:114:22FVMWLT1:2:1211:31703:21128/151‑1 (MQ=60)

                                                  GGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCC                                                                                                 <  LH00941:114:22FVMWLT1:2:2120:25536:13874/151‑1 (MQ=60)

                                                   GGCGGACATGAGCAGCTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                <  LH00941:114:22FVMWLT1:2:2110:39783:19975/151‑1 (MQ=60)

                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGATCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                <  LH00941:114:22FVMWLT1:2:1132:12242:1288/151‑1 (MQ=60)

                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                <  LH00941:114:22FVMWLT1:2:2215:10217:7325/151‑1 (MQ=60)

                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                <  LH00941:114:22FVMWLT1:2:2126:10957:1785/151‑1 (MQ=60)

                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                <  LH00941:114:22FVMWLT1:2:2258:43000:9759/151‑1 (MQ=60)

                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                <  LH00941:114:22FVMWLT1:2:2102:31157:26012/151‑1 (MQ=60)

                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                <  LH00941:114:22FVMWLT1:2:1268:6288:28382/151‑1 (MQ=60)

                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                <  LH00941:114:22FVMWLT1:2:1260:47142:23978/151‑1 (MQ=60)

                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                <  LH00941:114:22FVMWLT1:2:2268:40060:18342/151‑1 (MQ=60)

                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                <  LH00941:114:22FVMWLT1:2:2268:40069:18326/151‑1 (MQ=60)

                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                <  LH00941:114:22FVMWLT1:2:2270:15783:9887/151‑1 (MQ=60)

                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                <  LH00941:114:22FVMWLT1:2:1158:21644:13954/151‑1 (MQ=60)

                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                <  LH00941:114:22FVMWLT1:2:1206:31629:26380/151‑1 (MQ=60)

                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                <  LH00941:114:22FVMWLT1:2:1206:31481:26508/151‑1 (MQ=60)

                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                <  LH00941:114:22FVMWLT1:2:1224:23354:14002/151‑1 (MQ=60)

                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                <  LH00941:114:22FVMWLT1:2:1249:13379:9279/151‑1 (MQ=60)

                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                <  LH00941:114:22FVMWLT1:2:1127:34005:9615/151‑1 (MQ=60)

                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                <  LH00941:114:22FVMWLT1:2:1163:48464:14579/151‑1 (MQ=60)

                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC                                                                                                <  LH00941:114:22FVMWLT1:2:1144:49638:21032/151‑1 (MQ=60)

                                                        ACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCG                                                                                           <  LH00941:114:22FVMWLT1:2:1210:37314:4843/151‑1 (MQ=60)

                                                        ACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCG                                                                                           <  LH00941:114:22FVMWLT1:2:2208:49102:28654/151‑1 (MQ=60)

                                                        ACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCG                                                                                           <  LH00941:114:22FVMWLT1:2:2208:49111:28638/151‑1 (MQ=60)

                                                        ACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCG                                                                                           <  LH00941:114:22FVMWLT1:2:2216:42639:8814/151‑1 (MQ=60)

                                                         CATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCcaggtt                                                                                          <  LH00941:114:22FVMWLT1:2:2162:30039:17189/151‑7 (MQ=60)

                                                                   TCCTTTTTTCATGTGTCGCCGACGGCNGAGCAACACCGCTCGGGTGTGGGAGTGGCCTTGTTTCGCGAAAGGGCCGCG‑‑‑TA‑‑‑GCTTCC‑‑‑CCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGC                                                                                <  LH00941:114:22FVMWLT1:2:2267:27995:19191/151‑1 (MQ=60)

                                                                   TCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGNGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCcaggtttgttgcatac                                                                                <  LH00941:114:22FVMWLT1:2:2261:12769:9343/151‑17 (MQ=60)

                                                                            CGTGTGTCGCAGACAGAAGAGAAACACCGTTCAGGTGTGGGAGCGGCTTTGTGTCGAGAAGGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGTGGGTATGAAAAAAACCTGGGGTTGCGATGAAGCCCTTTCGCGACACAAGGCCCACAAAGG                                                                       <  LH00941:114:22FVMWLT1:2:2162:4402:14355/151‑1 (MQ=52)

                                                                            CATTTTTCGCCGACAGCATAGCAACACCGCTCAGGTGGGGGAGTGGCCTTGTTTCGAGAAGGGGCCGCATGGTG‑‑‑G‑‑‑CC‑‑‑CTGGCAGTTTCCGTGTGTATGCAACAACCTTGGGGCTGCTGTGCATCCCTTTCGCGACACAAGGCCCACAAAGG                                                                       <  LH00941:114:22FVMWLT1:2:2263:33921:16709/151‑1 (MQ=55)

                                                                              TGTGTCGCCGACAGCATAGCAACACCGCTCAGGGGGGGGAGCGGCCTTGTGTCGTGAAAGGGCCGCA‑‑‑GA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTAGGCAACAAACCTGGGGCTGCTAGGCATCCCTTTCGCGACACAAGGCCCACAAAGGAC                                                                     <  LH00941:114:22FVMWLT1:2:1266:22827:12385/151‑1 (MQ=60)

                                                                                        ACAGCATAGCAACACTGCTCAGGTGGGGGAGCGGTTTTGTGTTGAGAAAGGGCTACA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAGTTTCCGGGTGGAGGAAAAAAACTGGGGGTTGCTATGCAGCCTTTTCGAGACACAAGGCCCACAAAGGACCGCGCAGGCT                                                           <  LH00941:114:22FVMWLT1:2:2226:22578:17557/151‑1 (MQ=29)

                                                                                        AAAGCAGAGCAACACCGCTTGGGTGTGGGAGTGGCTTTGGGTCGCGAAGGGGCCGCG‑‑‑TG‑‑‑G‑‑‑CGGCCCTGGCAATTTCCGCGTGGGTGCAACAAACTTGGGGCTGCTGTGCATCCTTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCT                                                           <  LH00941:114:22FVMWLT1:2:2226:22569:17573/151‑1 (MQ=47)

                                                                                              TAGCAACACCGCTCAGGTGTGGGAGCGGCCCTTTGTCGCGAAAAGGACGCA‑‑‑CA‑‑‑G‑‑‑CGGTCCCGGCAATTTCCccgtttgttccacaaaccctgggatgttttgtcgcccctttgtgacacaaagacccccaaagaccacgccggcttccggt                                                     >  LH00941:114:22FVMWLT1:2:2146:14747:26989/1‑71 (MQ=29)

                                                                                               AGCAACACCGCTCGGGTGGGGGAGCGGCTTTGTGTCGCGAAAGGGCCGCA‑‑‑GA‑‑‑G‑‑‑CGGCCCTGGCAATTTCCGTGTGTATGCAAAAAACTTGGGGTTGTTGTGAGGCCTTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC                                                    <  LH00941:114:22FVMWLT1:2:1133:41022:18086/151‑1 (MQ=53)

                                                                                               AGCAACACCGCTAAGGTGGGGGAGTGGCTTTGTGCCGCGAAGGGGCCGCG‑‑‑GA‑‑‑G‑‑‑CGGCCCCGGCACTTTCCGTGGGGATGAAACAAACTTGGGGTGGCTGTGCAGCCCTTTCGAGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC                                                    <  LH00941:114:22FVMWLT1:2:1161:48334:24058/151‑1 (MQ=36)

                                                                                                     ccctctcgGGTGTGGGGGCGGCTTTGNGTCGCGAAGGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGTGGGTATGCAACAAACTTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAAC                                              <  LH00941:114:22FVMWLT1:2:2267:31462:22249/143‑1 (MQ=60)

                                                                                                     CCCGCTCGGGTTTGGGAGCGGCCTTGTGTCGCGAAAGTGCCGCA‑‑‑TAGTGG‑‑‑CC‑‑‑CCGGCACTTTCNGCGTGGATACAACAAACCTGGGGCTGNGATGCAGCCCTTTCGCGACANAAGGCCCACAAAGGACCGCGCAGGCTTCNGGCCCGGAAC                                              <  LH00941:114:22FVMWLT1:2:1164:27820:29711/151‑1 (MQ=60)

                                                                                                     CCCGCTCAGGTGGGGGAGCGGCCTTGTGTCGGGAAAGGGCCGAAGAGTG‑‑‑G‑‑‑CC‑‑‑CCGGCAATTTCTGCGGGTATGAAACAAACTTGGGGCTGTTAGGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAAC                                              <  LH00941:114:22FVMWLT1:2:2235:12898:12161/151‑1 (MQ=60)

                                                                                                     ACCTCTCGGGTGTGGGAGTGGCTTTGTGTCGAGAAGGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCTGGCAATTTCCGCGTGTGGGNAACAAACTTGGGGTTGCTATGCATCCTTTTCTCAACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAAC                                              <  LH00941:114:22FVMWLT1:2:2249:36270:17797/151‑1 (MQ=59)

                                                                                                          TCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCAGAGTG‑‑‑G‑‑‑CC‑‑‑CCGGCAGTTTCCGTGTGGGTGCAAAAAACTGGGGGTGGTTAGGAAGCCTTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGNCC                                         <  LH00941:114:22FVMWLT1:2:2254:38997:1128/151‑1 (MQ=31)

                                                                                                            ggtttgttgcatacacgcggaaattgccgGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTAT‑CAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAG                                      >  LH00941:114:22FVMWLT1:2:1162:31980:25644/30‑151 (MQ=60)

                                                                                                               tgggggagtggcttgGTGTCGAGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGAAGTTTCCGTGTGTATGAAAAAAACCTGGGGCTGCTAGGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGC                                    <  LH00941:114:22FVMWLT1:2:1228:34236:21897/136‑1 (MQ=60)

                                                                                                               gggGGGAGCGGCCTTGTGTCGAGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTCCCGCGTGTATGCAACAAACCTGGGGCTGTTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGC                                    <  LH00941:114:22FVMWLT1:2:1151:51404:14867/148‑1 (MQ=60)

                                                                                                               TTTGGGAGCGGCCTTGTGTCGAGAAAGGTCCGAA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAACCTGGGGGCTGCCATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCATGC                                    <  LH00941:114:22FVMWLT1:2:2250:13194:22153/151‑1 (MQ=60)

                                                                                                               TGTGGGAGTGGCCTTGTGTCGCGAAGGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGTGTGTATGCAACAACCCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGC                                    <  LH00941:114:22FVMWLT1:2:2256:38035:26044/151‑1 (MQ=60)

                                                                                                               TGTGGGAGCGGCTTTGTGTCGCGAAAGGGCCGCA‑‑‑GA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGTGTGTATGCAACAAACCTGGGGCTGCGATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGC                                    <  LH00941:114:22FVMWLT1:2:2250:13176:22153/151‑1 (MQ=60)

                                                                                                               TGTGGGAGCGGCTTTGTGTCGAGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCTGGCAATTTCCGTGGGGAGGAAACAAACTGGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGC                                    <  LH00941:114:22FVMWLT1:2:2163:39367:1480/151‑1 (MQ=60)

                                                                                                               TGTGGGAGCGGCCTTGTTCCGCAAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTGGGCAAAAACCTTGGGGCTGCTGTGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGC                                    <  LH00941:114:22FVMWLT1:2:2258:24399:25612/151‑1 (MQ=60)

                                                                                                               TGTGGGAGCGGCCTTGTGTCGCGAAAGGTCCGCA‑‑‑CA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATAAAACAACCTTGGGTTTGCTATGCATCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGC                                    <  LH00941:114:22FVMWLT1:2:1228:34227:21913/151‑1 (MQ=60)

                                                                                                                               gaaattgccgGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGC                    >  LH00941:114:22FVMWLT1:2:2228:32627:18630/11‑151 (MQ=60)

                                                                                                                                  CTCGAAAGGGCCGCA‑‑‑TAGTGG‑‑‑CC‑‑‑CCGGCAGTTTCTGCGTGGAGGCAAAAAACCTGGGGCTGCTGTGCAGCCCTTTCGCAACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGC                 <  LH00941:114:22FVMWLT1:2:1256:30658:1673/151‑1 (MQ=60)

                                                                                                                                  CGCGAAAGGGCCACA‑‑‑TAGTGG‑‑‑CC‑‑‑CCGGCAATTTCCGCGTGCAGGCAACAAACCGGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGC                 <  LH00941:114:22FVMWLT1:2:1256:30649:1657/151‑1 (MQ=60)

                                                                                                                                         GGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCA          >  LH00941:114:22FVMWLT1:2:1123:7185:4539/1‑151 (MQ=60)

                                                                                                                                         GGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCA          >  LH00941:114:22FVMWLT1:2:1128:32451:6476/1‑151 (MQ=60)

                                                                                                                                         GGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCA          >  LH00941:114:22FVMWLT1:2:1128:32461:6460/1‑151 (MQ=60)

                                                                                                                                         GGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCA          >  LH00941:114:22FVMWLT1:2:1112:24566:19335/1‑151 (MQ=60)

                                                                                                                                         GGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCA          >  LH00941:114:22FVMWLT1:2:1168:41197:2121/1‑151 (MQ=60)

                                                                                                                                         GGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCA          >  LH00941:114:22FVMWLT1:2:2142:16134:15844/1‑151 (MQ=60)

                                                                                                                                         GGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCA          >  LH00941:114:22FVMWLT1:2:1222:38137:26252/1‑151 (MQ=60)

                                                                                                                                         GGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCA          >  LH00941:114:22FVMWLT1:2:1225:44211:21016/1‑151 (MQ=60)

                                                                                                                                          GGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCAT         >  LH00941:114:22FVMWLT1:2:2221:49666:23002/1‑151 (MQ=60)

                                                                                                                                          GGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCAT         >  LH00941:114:22FVMWLT1:2:2132:28005:28654/1‑151 (MQ=60)

                                                                                                                                          GGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCAT         >  LH00941:114:22FVMWLT1:2:1238:47863:15876/1‑151 (MQ=60)

                                                                                                                                           GCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATC        >  LH00941:114:22FVMWLT1:2:2159:29428:18886/1‑151 (MQ=60)

                                                                                                                                           GCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATC        >  LH00941:114:22FVMWLT1:2:2159:29419:18870/1‑151 (MQ=60)

                                                                                                                                           GCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATC        >  LH00941:114:22FVMWLT1:2:2136:33478:24427/1‑151 (MQ=60)

                                                                                                                                            CCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCC       >  LH00941:114:22FVMWLT1:2:2211:44692:9967/1‑151 (MQ=60)

                                                                                                                                            CCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCC       >  LH00941:114:22FVMWLT1:2:2126:14146:19863/1‑151 (MQ=60)

                                                                                                                                              GCA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAG     >  LH00941:114:22FVMWLT1:2:1250:14063:7453/1‑151 (MQ=60)

                                                                                                                                               CA‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGC    >  LH00941:114:22FVMWLT1:2:2201:23780:22553/1‑151 (MQ=60)

                                                                                                                                                A‑‑‑TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCA   >  LH00941:114:22FVMWLT1:2:1165:35160:3578/1‑151 (MQ=60)

                                                                                                                                                    TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGTGGGTATGCAACAAACCTGGGGCTGCCATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCAC  <  LH00941:114:22FVMWLT1:2:2218:16606:1192/151‑1 (MQ=60)

                                                                                                                                                    TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCAC  >  LH00941:114:22FVMWLT1:2:1246:28652:28174/1‑151 (MQ=60)

                                                                                                                                                    TA‑‑‑G‑‑‑CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCAC  >  LH00941:114:22FVMWLT1:2:2134:10263:24539/1‑151 (MQ=60)

                                                                                                                                                    TA‑‑‑G‑‑‑CGGCCCCGGAAATTTCCGCGTGTAGGCAACAAACCTGGGGCTGCTATGCATCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCAC  <  LH00941:114:22FVMWLT1:2:1253:48658:21128/151‑1 (MQ=60)

TACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CG‑‑‑CCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCAC  >  AE015451_phaM1/5555459‑5555751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`