Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F35 I1 R1 74 857.2 37938082 99.4% 37710453 136.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451_phaM1 5,555,608 +GCC intergenic (‑84/+87) PP_4887 CDS (conserved protein of unknown function) ← / ← PP_4888 CDS (putative methyl‑accepting chemotaxis transducer) –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM15,555,6081.G92.2% 760.9 / 78.5 278intergenic (‑84/+87)PP_4887 CDS (conserved protein of unknown function)/PP_4888 CDS (putative methyl‑accepting chemotaxis transducer)–/–
Reads supporting (aligned to +/- strand):  ref base . (0/21);  new base G (11/236);  total (11/267)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.05e-01
*AE015451_phaM15,555,6082.C92.5% 891.3 / 71.8 278intergenic (‑84/+87)PP_4887 CDS (conserved protein of unknown function)/PP_4888 CDS (putative methyl‑accepting chemotaxis transducer)–/–
Reads supporting (aligned to +/- strand):  ref base . (0/21);  new base C (11/246);  total (11/267)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*AE015451_phaM15,555,6083.C85.6% 852.5 / 154.7 278intergenic (‑84/+87)PP_4887 CDS (conserved protein of unknown function)/PP_4888 CDS (putative methyl‑accepting chemotaxis transducer)–/–
Reads supporting (aligned to +/- strand):  ref base . (0/40);  new base C (11/227);  total (11/267)
Fisher's exact test for biased strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 294 total aligned reads displayed.

TGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑G‑‑‑CC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAG  >  AE015451_phaM1/5555472‑5555727
                                                                                                                                                 |||                                                                                                                        
tgCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑g‑c‑g                                                                                                                             <  1:9150785/137‑1 (MQ=255)
tgCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑g‑c‑g                                                                                                                             <  1:10117573/137‑1 (MQ=255)
tgCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑g‑c‑g                                                                                                                             <  2:2837638/137‑1 (MQ=255)
    ggAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑g‑c‑ggccc                                                                                                                         <  2:11174582/137‑5 (MQ=255)
                cGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCcg                                                                                                            <  1:3270068/137‑1 (MQ=38)
                cGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCcg                                                                                                            <  2:942978/137‑1 (MQ=38)
                cGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCcg                                                                                                            <  1:1270916/137‑1 (MQ=38)
                   ccTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCgt                                                                                                         <  1:10230755/137‑1 (MQ=38)
                   ccTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCgt                                                                                                         <  1:8106876/137‑1 (MQ=38)
                   ccTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCgt                                                                                                         <  2:17632760/137‑1 (MQ=38)
                   ccTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCgt                                                                                                         <  2:18012241/137‑1 (MQ=38)
                   ccTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCgt                                                                                                         <  1:14537057/137‑1 (MQ=38)
                   ccTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCgt                                                                                                         <  1:14124466/137‑1 (MQ=38)
                   ccTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCgt                                                                                                         <  2:8552340/137‑1 (MQ=38)
                   ccTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCgt                                                                                                         <  2:8913862/137‑1 (MQ=38)
                        gCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATg                                                                                                    <  2:15734436/137‑1 (MQ=38)
                         cAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGc                                                                                                   <  2:12310166/137‑1 (MQ=38)
                         cAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGc                                                                                                   <  1:11201551/137‑1 (MQ=38)
                         cAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGc                                                                                                   <  1:5163168/137‑1 (MQ=38)
                          aGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGca                                                                                                  <  2:422050/137‑1 (MQ=38)
                              aTTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGcaacaa                                                                                              <  2:15606153/137‑1 (MQ=38)
                              aTTAGTCGGGCGGACATGAGCAACCCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGcaacaa                                                                                              <  2:16947989/137‑1 (MQ=38)
                               ttAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGc                                                                                                   >  1:1662503/1‑131 (MQ=38)
                               ttAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGc                                                                                                   <  2:1662503/131‑1 (MQ=38)
                               ttAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACaaa                                                                                             >  2:9712553/1‑137 (MQ=38)
                                 aGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAAcc                                                                                           <  1:8913921/137‑1 (MQ=38)
                                 aGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAAcc                                                                                           <  2:7128336/137‑1 (MQ=38)
                                 aGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAAcc                                                                                           <  2:231460/137‑1 (MQ=38)
                                 aGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAAcc                                                                                           <  1:2868859/137‑1 (MQ=38)
                                 aGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAAcc                                                                                           <  1:1722163/137‑1 (MQ=38)
                                 aGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTGGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAAcc                                                                                           <  1:7790921/137‑1 (MQ=38)
                                   tCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTg                                                                                         <  2:9289168/137‑1 (MQ=38)
                                   tCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTg                                                                                         <  2:14984636/137‑1 (MQ=38)
                                    cGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgg                                                                                        <  1:13610137/137‑1 (MQ=38)
                                    cGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgg                                                                                        <  2:7712137/137‑1 (MQ=38)
                                     gggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTggg                                                                                       <  2:5584445/137‑1 (MQ=38)
                                      ggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                      <  2:16204980/137‑1 (MQ=38)
                                      ggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                      <  2:4612775/137‑1 (MQ=38)
                                      ggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                      <  1:2349252/137‑1 (MQ=38)
                                      ggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                      <  1:9712553/137‑1 (MQ=38)
                                      ggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                      <  2:16102956/137‑1 (MQ=38)
                                      ggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                      <  1:17864160/137‑1 (MQ=38)
                                      ggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                      <  2:8351/137‑1 (MQ=38)
                                      ggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                      <  1:13122712/137‑1 (MQ=38)
                                      ggCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTgggg                                                                                      <  1:11712647/137‑1 (MQ=38)
                                         ggACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGgctg                                                                                   <  1:572903/137‑1 (MQ=38)
                                            cATGAGCAACTTCTTTTGTCATGTGTCGCCGACAGCGTAGCAACACCGCTCGGGTGTGGGAGCGGCTTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTa                                                                                <  2:12606733/137‑1 (MQ=37)
                                                 gCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAg                                                                           <  2:18141697/137‑1 (MQ=38)
                                                   aaCTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑T‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGcc                                                                         <  2:18764647/137‑1 (MQ=38)
                                                      tCCTTTTGTCGTGTTTCTCCGACAGCATAGCAACACCGCTCGGGTGTGGGAGCGGCCTTGTGTCGAGAAGGGGCCGCA‑‑‑TATCGG‑C‑C‑‑‑CC‑GGCAATTCCCGCGGGTATGCAACAACCCTGGGGCTGCTATGCAGCCCtt                                                                      <  2:12099080/137‑1 (MQ=25)
                                                      tCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAGGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAGTTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCtt                                                                      <  2:1397225/137‑1 (MQ=38)
                                                      tCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCtt                                                                      <  2:13268172/137‑1 (MQ=38)
                                                             ttCATGTGTCGCCGACGACATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑GA‑‑‑G‑C‑GGCCCC‑GGCACTTTCCGCGTGTGTGCAACAAACCTGGGGCTGCTATTCACCCCTTTCGCGac                                                               <  2:15515124/136‑1 (MQ=25)
                                                               catttttCGCCGAAAGCATAGCAACACCGTTCAGGTGTGGGAGCGGTCTTGTGTCGCGAAAGGGCCGCA‑‑‑TG‑‑‑G‑G‑GGC‑CCTGGCAATTTCCGCGTGTGTGCAACAAACTTGGGGCTGCTATGCAGCCCTTTCGCGacac                                                             <  2:14344875/131‑1 (MQ=25)
                                                               cATGTTTCGCCGACAGCATATCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAGGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGacac                                                             <  2:17290555/137‑1 (MQ=37)
                                                               cATGTTTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGTGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCGATGCAGCCCTTTCGCGacac                                                             <  2:2680562/137‑1 (MQ=37)
                                                               cATGTGTCGCTAACAGCATAGCAACACTGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑T‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGTTGCTATGCAGCCCTTTCGCGacac                                                             <  2:10232581/137‑1 (MQ=37)
                                                               cATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGTGGCCTTGTGTCGTGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCTGCGTGTATACAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGacac                                                             <  2:13734205/137‑1 (MQ=38)
                                                               cATGTGTCGCCGACAGCACAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGTTATGCAGCCCTTTCGCGacac                                                             <  2:6179132/137‑1 (MQ=37)
                                                                 tgtgTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTTGCGAAGGGTCCGCA‑‑‑TA‑‑‑G‑T‑GGCCCC‑GGCAATTTCCGAGGGTATGCAACAAACTTGGGGCTGCGATGCAGCCCTTTCGCGACACaa                                                           <  2:4122140/137‑1 (MQ=37)
                                                                 tgtgTCGCCGACAGCACAGCAACCCCGTTCGGGTGTGGAAGCGGCTTTGTTTCGCGAAGGGTCCGCA‑‑‑TATT‑GTC‑C‑‑‑TC‑GGCAGTTTCCGCGGGTGTACAGTAAACCTGGGGTTGCTGTGCAGCCCTTTCGCGACACaa                                                           <  2:7656122/137‑1 (MQ=255)
                                                                      cGCCAACAGCATAGCAACACCCCTCAGGTGTGGGGGCGGCCTTGTGCCGGAAAGGGGCCGCAGAGTG‑‑‑G‑C‑C‑‑‑CC‑GGCAGTTTCCGTGTGTGTGAAAAAAACTTGGGGCTGCTATGCAGCCTTTTCGCGACACAAGGccc                                                      <  2:6206024/137‑1 (MQ=255)
                                                                           aaagcaaagcaaCACCGCTCAGGTGTGGAAGGGACTTGGGGTAGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCGACGGGGAGGAAAAAAACCGGGGGCTGCGATGAAGCCCTTTCGCGAAACAAGGCCCACaaa                                                 <  2:15950442/135‑1 (MQ=255)
                                                                                atggCAACACCGCTAAGGTGGGGGAGCGGCCTTGTTTCGCGAAAGGGCCGCAGGGTG‑‑‑G‑C‑C‑‑‑CC‑GGCAATTTCCGCGTGTATGCAACAAACTTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGAcc                                            <  2:2075385/134‑1 (MQ=37)
                                                                                agagaaaCACCTCTCGGGTGGGGGAGGGGCTTTGTGTCGCGAAGGGTCCGCG‑‑‑GG‑‑‑G‑C‑GGC‑CCTGGAAGTTCCCGTGTGTATGAAAAAAACTTGGGGCTGCTATGCAGCCTTTTCGCGACACAAGGCCCACAAAGGAcc                                            <  1:13363630/132‑1 (MQ=255)
                                                                                agagaaaCACCGCTCAGGTGTGGGAGCGGCCTTGTTTCGCGAAAGTGCCGCA‑‑‑GA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGAAAAAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGAcc                                            <  2:7902684/132‑1 (MQ=25)
                                                                                aTAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCTCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAGTTTCCGTGTGTGTGCAACAAACCTGGGGCGGCTAGGCAGCCCTTTCGCGACACAAGGCCCACAAAGGAcc                                            <  2:5784301/137‑1 (MQ=25)
                                                                                aTAGCAACACCGCTAAGGTGTGGGAGCGGCCTTGTTTCGCGAAGGGGCTGCA‑‑‑GA‑‑‑G‑C‑GGC‑CCTGGCAATTTCCGTGGGGGGGAAACAAACTTGGGGCGGCTATGCGGCCTTTTCGGGACACAAGGCCCACAAAGGAcc                                            <  2:6371833/137‑1 (MQ=255)
                                                                                aTAGCAACACCCCTCAGGTGGGGGAGTGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TATCGG‑C‑C‑‑‑CC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCGGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGAcc                                            <  2:3627116/137‑1 (MQ=37)
                                                                                aTAGCAACACCCCCCAGGTGTGGGAGCGACCTTGTGCCGCAAAAGGACCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGAAATTCCCGCGGGTATGCAACAAACCTGGGGCGGCTATGCAGCCCTTTCACGACACAAGGCCCACAAAGGAcc                                            <  2:12972521/137‑1 (MQ=255)
                                                                                aTAACAACACCGCTCAGGTGTGGGAGCGGCCTTGGGTCGCGAAAGGGCCGCG‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGAcc                                            <  2:10964588/137‑1 (MQ=37)
                                                                                  agaaaCACCGCTCGGGTGTGGGAGCGGCTTTGTGTCGAGAAGGGGCCGCA‑‑‑GA‑‑‑G‑C‑GGCCCC‑GGCAGTTTCCGTGGGGATGAAAAAAACTTGGGGCTGCGATGAAGCCCTTTCGCGACACAAGGCCCACAAAGGACcgc                                          <  2:10467218/134‑1 (MQ=16)
                                                                                  aacAACACCGCTAAGGGGGGGGAGCGGCCTTGTGTCGAGAAGGGGCCGCAGAGTG‑‑‑G‑C‑C‑‑‑CC‑GGCAGTTTCTGTGTGGAGGAAAAAAACCTGGGGTTGCGGGGAAGCCTTTTCGCGACACAAGGCCCACAAAGGACcgc                                          <  2:9049202/135‑1 (MQ=255)
                                                                                  aaaaaCACCGCTAAGGTGGGGGAGCGGCTTTGTGTTGCGAAAGGGCAGCA‑‑‑GA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGTGTGTGGGCAACAAACTTGGGGCTGTTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACcgc                                          <  2:3362179/134‑1 (MQ=21)
                                                                                  aGCAACACCGCTCAGGTGGGGGATCGGCCTTGTGTAGCGAAAGGGCCGAA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAAAAAACCTGGGGCTGCTAGGCGGCCTTTTCGCGACACAAGGCCCACAAAGGACcgc                                          <  2:10051444/137‑1 (MQ=255)
                                                                                     aaCACCGTTCGGGGGTGGGAGTGGCTTTGTGTCGGGAAAGGGCTGCA‑‑‑GA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGGGGGCAACAAATTGGGGGTTGCGATGCAGCCCTTTCGTGACACAAGGCCCACAAAGGACCGCGCa                                       <  1:15258028/137‑1 (MQ=255)
                                                                                       cccctcTCGGGTTTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑T‑GGCCCC‑GGCAATTTCCGTGTGTGTGCAACAAACCTGGGGTTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAgg                                     <  2:690413/132‑1 (MQ=25)
                                                                                        acCGCTCGGGTGTGGGAGTGGCTTTGTGTTGCGAAAGGGCCGCA‑‑‑TG‑‑‑G‑C‑GGC‑CCTGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGc                                    <  2:655177/137‑1 (MQ=37)
                                                                                          cGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTggg                                                                                       >  2:3107742/1‑84 (MQ=18)
                                                                                             tcgggTGGGGGAGCGGCTTTGTTTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACTTGGGGCTGCGGTGCAGCCCTTTCGCGAAACAAGGCCCACAAAGGACCGCGCAGGCTTCAg                               <  2:1981446/134‑1 (MQ=255)
                                                                                             taaGGTGTGGGAGCGGCCTTTTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGGAGGCAAAAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAg                               <  2:6756902/135‑1 (MQ=21)
                                                                                             ccAGGTGTGGGAGCGGCCTTGTTCCGCGAAAGGGCCGAA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTAGGAAACAAACCGGGGGTTGCTAGGCACCCCTTTCGCGAAACAAGGCCCACAAAGGACCGCGCAGGCTTCAg                               <  2:3911670/136‑1 (MQ=255)
                                                                                             ccAGGTGGGGGAGCGGCTTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTCCCGCGTGTGTGAAAAAAACCTGGGGTGGCTGTGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAg                               <  1:2017607/136‑1 (MQ=25)
                                                                                                  tgtgGGAGTGGCTTTGTTTTGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAGTTTCCGTGGGTATGCAACAAACCGGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCg                          <  1:18453142/137‑1 (MQ=25)
                                                                                                  tgtgGGAGCGGCCTTGTGTCGCGAAGGGGCCGCA‑‑‑TAGTGG‑C‑C‑‑‑CC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCTTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCg                          <  2:17314359/137‑1 (MQ=38)
                                                                                                  tgtgGGAGCGGCCTTGTGTCGCAAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GTCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGAAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCg                          <  1:9034022/137‑1 (MQ=38)
                                                                                                  tgtgGGAGCGGCCTTGTGTCGCAAAAGGACCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GACAATTTCCGCGTGTATGCAACAACCCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCg                          <  2:10692372/137‑1 (MQ=38)
                                                                                                  tgggggAGCGGTCTTGTGTCGAGAAGGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAGTTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCg                          <  2:15138254/134‑1 (MQ=37)
                                                                                                   ttgGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGTCTGTTATGCATCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCgg                         <  2:17478410/136‑1 (MQ=37)
                                                                                                   gtgGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑T‑C‑GGCCCC‑GGCAATTTCCGCGTGTATACAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCgg                         <  2:5470737/137‑1 (MQ=38)
                                                                                                       gggCGGCCGTGTGTCGCGAAAGGGCCACA‑‑‑TA‑‑‑G‑CTGCC‑CC‑GACAATTTCCGCGTGTGTGAAACAAACCGGGTGCTGTTATGCAGCCTTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACt                     <  1:15943428/135‑1 (MQ=255)
                                                                                                       gAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑GA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGTGTGTATGAAACAAACTTGGGGTTGCTGTGCAGCCTTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACt                     <  2:6288510/137‑1 (MQ=25)
                                                                                                                  tttCGCGAAGGGGCCGCA‑‑‑GG‑‑‑G‑C‑GTCCCC‑GGCACTTTCCGTGTGTATGAAACAAACTTGGGGTTGCTATGCAGCCTTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTg          <  1:4349788/135‑1 (MQ=255)
                                                                                                                  tgtAGCGAAAGGGCCACA‑‑‑TA‑‑‑G‑C‑GGC‑CCTGGCAATTTCCGCGTGGATGCAACAAACTTGGGGCTGCTAGGAAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTg          <  2:11380129/137‑1 (MQ=255)
                                                                                                                    tCGCGAAGGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATACAACAAACCTGGGGTTGCTGGGCAGCCTTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGgc        <  2:14562415/137‑1 (MQ=255)
                                                                                                                        gAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAGTTTCCGCGTGTATGCAACAAACCTGGGTCTGCTATGCAACCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGgcgcgc    <  2:7450597/137‑1 (MQ=255)
                                                                                                                         aaaGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAACCCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCa   <  2:4471974/137‑1 (MQ=255)
                                                                                                                          aaaggCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAACCCGGGGCTGCTCTGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAAGCTTGCGCGCAg  >  2:18359889/4‑137 (MQ=255)
                                                                                                                          aaGGGCCGCA‑‑‑TA‑‑‑G‑C‑GGCCCC‑GGCAATTTCCGCGTGTATGCAACAAAACTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCACGCAGGCTTCCGGCCCGGAACTGGCCCCGGCTGGCGCGCAg  >  2:10837388/1‑137 (MQ=255)
                                                                                                                          aaGGGCCACA‑‑‑TA‑‑‑G‑C‑GGCCCC‑AGCAATTTCCGCGTGTATGCCACAAACCTGGGGCTGCTCTGCAGCCCTTTCGCGACACACGGCCCACAAAGGACCGCGCAGGCTTCCGGCCCTGAACTGGCCCAGGCTGGCGCGCAg  >  2:1476296/1‑137 (MQ=255)
                                                                                                                                                 |||                                                                                                                        
TGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑C‑G‑‑‑CC‑GGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAG  >  AE015451_phaM1/5555472‑5555727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CG‑‑‑CC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCTTGCT  >  AE015451_phaM1/5555459‑5555757
                                                                                                                                                              |                                                                                                                                                            
TACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CG‑‑‑G                                                                                                                                                          <  LH00941:114:22FVMWLT1:2:2208:25555:23578/151‑1 (MQ=60)
  CGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGgcc                                                                                                                                                           <  LH00941:114:22FVMWLT1:2:1110:35077:14995/151‑4 (MQ=60)
   GCTGTCACTGTGCAGGAAATTCAGGTCTGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGgccc                                                                                                                                                          <  LH00941:114:22FVMWLT1:2:1103:49287:2906/151‑5 (MQ=60)
   GCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGgccc                                                                                                                                                          <  LH00941:114:22FVMWLT1:2:1107:48649:7085/151‑5 (MQ=60)
   GCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGgccc                                                                                                                                                          <  LH00941:114:22FVMWLT1:2:2159:2146:18230/151‑5 (MQ=60)
   GCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGgccc                                                                                                                                                          <  LH00941:114:22FVMWLT1:2:1159:34227:17878/151‑5 (MQ=60)
   GCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGgccc                                                                                                                                                          <  LH00941:114:22FVMWLT1:2:1208:43490:20183/151‑5 (MQ=60)
    CTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGgcctg                                                                                                                                                         >  LH00941:114:22FVMWLT1:2:1217:46864:14467/1‑146 (MQ=60)
        CACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCA                                                                                                                                                  <  LH00941:114:22FVMWLT1:2:2264:5696:17589/151‑1 (MQ=60)
        CACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATGAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCA                                                                                                                                                  <  LH00941:114:22FVMWLT1:2:2118:29317:14627/151‑1 (MQ=60)
         ACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAA                                                                                                                                                 <  LH00941:114:22FVMWLT1:2:1260:51256:10928/151‑1 (MQ=60)
         ACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAA                                                                                                                                                 <  LH00941:114:22FVMWLT1:2:2252:33958:25195/151‑1 (MQ=60)
          CTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGgccctttcgcg                                                                                                                                                   <  LH00941:114:22FVMWLT1:2:1165:16513:4139/151‑12 (MQ=60)
          CTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAAT                                                                                                                                                <  LH00941:114:22FVMWLT1:2:1215:30334:25516/151‑1 (MQ=60)
            GTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACGTGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGgccctttcgcgac                                                                                                                                                 <  LH00941:114:22FVMWLT1:2:1230:26729:5788/151‑14 (MQ=60)
             TGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTC                                                                                                                                             <  LH00941:114:22FVMWLT1:2:2110:51071:17621/151‑1 (MQ=60)
             TGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTC                                                                                                                                             <  LH00941:114:22FVMWLT1:2:1266:8581:5708/151‑1 (MQ=60)
             TGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTC                                                                                                                                             <  LH00941:114:22FVMWLT1:2:1265:34236:19110/151‑1 (MQ=60)
             TGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATGTC                                                                                                                                             <  LH00941:114:22FVMWLT1:2:2248:36954:23530/151‑1 (MQ=60)
             TGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATGTC                                                                                                                                             <  LH00941:114:22FVMWLT1:2:1266:8599:5708/151‑1 (MQ=60)
                 GGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCG                                                                                                                                         <  LH00941:114:22FVMWLT1:2:2228:45117:11440/151‑1 (MQ=60)
                  GAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGT                                                                                                                                        <  LH00941:114:22FVMWLT1:2:2162:8895:13746/151‑1 (MQ=60)
                        CAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGC                                                                                                                                  <  LH00941:114:22FVMWLT1:2:2120:21099:17781/151‑1 (MQ=60)
                        CAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGC                                                                                                                                  <  LH00941:114:22FVMWLT1:2:2149:22504:11056/151‑1 (MQ=60)
                        CAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGC                                                                                                                                  <  LH00941:114:22FVMWLT1:2:2149:22485:11056/151‑1 (MQ=60)
                        CAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGC                                                                                                                                  <  LH00941:114:22FVMWLT1:2:2254:15977:21817/151‑1 (MQ=60)
                          GGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAA                                                                                                                                <  LH00941:114:22FVMWLT1:2:1119:31952:24122/151‑1 (MQ=60)
                           GTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAAC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1158:35234:23498/151‑1 (MQ=60)
                           GTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAAC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1243:26119:16068/151‑1 (MQ=60)
                           GTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAAC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1247:8331:22089/151‑1 (MQ=60)
                           GTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAAC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1247:8350:22089/151‑1 (MQ=60)
                             CGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAANAA                                                                                                                             <  LH00941:114:22FVMWLT1:2:1120:46578:1160/151‑1 (MQ=60)
                             CGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAA                                                                                                                             <  LH00941:114:22FVMWLT1:2:1149:32017:16100/151‑1 (MQ=60)
                              GGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAA                                                                                                                            >  LH00941:114:22FVMWLT1:2:1125:4467:23690/1‑151 (MQ=60)
                                CCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACC                                                                                                                          <  LH00941:114:22FVMWLT1:2:2130:4143:26012/151‑1 (MQ=60)
                                CCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACC                                                                                                                          <  LH00941:114:22FVMWLT1:2:2225:1166:5772/151‑1 (MQ=60)
                                CCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACC                                                                                                                          <  LH00941:114:22FVMWLT1:2:2112:35724:9102/151‑1 (MQ=60)
                                CCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACC                                                                                                                          <  LH00941:114:22FVMWLT1:2:1116:11170:9839/151‑1 (MQ=60)
                                CCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACC                                                                                                                          <  LH00941:114:22FVMWLT1:2:1250:48020:24667/151‑1 (MQ=60)
                                CCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACC                                                                                                                          <  LH00941:114:22FVMWLT1:2:1253:44137:15347/151‑1 (MQ=60)
                                CCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACC                                                                                                                          <  LH00941:114:22FVMWLT1:2:2206:25573:6444/151‑1 (MQ=60)
                                CCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACC                                                                                                                          <  LH00941:114:22FVMWLT1:2:2203:13749:12097/151‑1 (MQ=60)
                                CCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACC                                                                                                                          <  LH00941:114:22FVMWLT1:2:2170:25055:3626/151‑1 (MQ=60)
                                CCGGGGCAGCGAGTAGTAGGGCGGACAGGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACC                                                                                                                          <  LH00941:114:22FVMWLT1:2:1219:38858:19175/151‑1 (MQ=60)
                                     GCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGG                                                                                                                    <  LH00941:114:22FVMWLT1:2:1207:21875:26268/151‑1 (MQ=60)
                                     GCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGG                                                                                                                    <  LH00941:114:22FVMWLT1:2:2265:52097:15043/151‑1 (MQ=60)
                                     GCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGG                                                                                                                    <  LH00941:114:22FVMWLT1:2:2230:3912:24203/151‑1 (MQ=60)
                                      CAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGC                                                                                                                   <  LH00941:114:22FVMWLT1:2:2165:33718:24363/151‑1 (MQ=60)
                                      CAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGC                                                                                                                   <  LH00941:114:22FVMWLT1:2:1117:35345:17477/151‑1 (MQ=60)
                                      CAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGC                                                                                                                   <  LH00941:114:22FVMWLT1:2:2258:5770:15347/151‑1 (MQ=60)
                                      CAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGC                                                                                                                   <  LH00941:114:22FVMWLT1:2:1263:30260:14499/151‑1 (MQ=60)
                                      CAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGC                                                                                                                   <  LH00941:114:22FVMWLT1:2:1212:27478:19415/151‑1 (MQ=60)
                                      CAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGC                                                                                                                   <  LH00941:114:22FVMWLT1:2:1134:11687:17653/151‑1 (MQ=60)
                                      CAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGC                                                                                                                   <  LH00941:114:22FVMWLT1:2:1207:30880:19703/151‑1 (MQ=60)
                                      CAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGC                                                                                                                   <  LH00941:114:22FVMWLT1:2:1206:34208:12849/151‑1 (MQ=60)
                                      CAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGC                                                                                                                   <  LH00941:114:22FVMWLT1:2:2126:43962:21865/151‑1 (MQ=60)
                                      CAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGC                                                                                                                   <  LH00941:114:22FVMWLT1:2:2139:11539:14931/151‑1 (MQ=60)
                                      CAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGC                                                                                                                   <  LH00941:114:22FVMWLT1:2:1137:4365:16596/151‑1 (MQ=60)
                                      CAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGC                                                                                                                   <  LH00941:114:22FVMWLT1:2:2162:17715:27837/151‑1 (MQ=60)
                                      CAGCAATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGC                                                                                                                   <  LH00941:114:22FVMWLT1:2:2108:14488:10335/151‑1 (MQ=60)
                                              AGTCGGGCGGACATGAGCAGCTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGC                                                                                                           <  LH00941:114:22FVMWLT1:2:2156:50599:2713/151‑1 (MQ=60)
                                              AGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGgcccctgtctcttatacacatctccgagcccacgagacaagatacac                                                                                                               >  LH00941:114:22FVMWLT1:2:1215:27394:24074/1‑104 (MQ=60)
                                              AGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGC                                                                                                           <  LH00941:114:22FVMWLT1:2:2245:37592:11825/151‑1 (MQ=60)
                                              AGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGC                                                                                                           <  LH00941:114:22FVMWLT1:2:2224:3737:14547/151‑1 (MQ=60)
                                              AGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGC                                                                                                           <  LH00941:114:22FVMWLT1:2:2206:51080:2521/151‑1 (MQ=60)
                                              AGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGC                                                                                                           <  LH00941:114:22FVMWLT1:2:1266:14729:10752/151‑1 (MQ=60)
                                              AGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGC                                                                                                           <  LH00941:114:22FVMWLT1:2:1262:4467:20231/151‑1 (MQ=60)
                                              AGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGC                                                                                                           <  LH00941:114:22FVMWLT1:2:1106:32941:6300/151‑1 (MQ=60)
                                              AGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGC                                                                                                           <  LH00941:114:22FVMWLT1:2:1143:46911:18006/151‑1 (MQ=60)
                                              AGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGC                                                                                                           <  LH00941:114:22FVMWLT1:2:1126:51126:20440/151‑1 (MQ=60)
                                              AGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTGGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGC                                                                                                           <  LH00941:114:22FVMWLT1:2:1246:46864:6556/151‑1 (MQ=60)
                                                TCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAG                                                                                                         <  LH00941:114:22FVMWLT1:2:1119:19028:18070/151‑1 (MQ=60)
                                                TCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTAGGCAG                                                                                                         <  LH00941:114:22FVMWLT1:2:2214:31925:26733/151‑1 (MQ=60)
                                                TCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGAAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAG                                                                                                         <  LH00941:114:22FVMWLT1:2:1203:19619:25436/151‑1 (MQ=60)
                                                 CGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGC                                                                                                        <  LH00941:114:22FVMWLT1:2:2159:3626:23194/151‑1 (MQ=60)
                                                 CGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGC                                                                                                        <  LH00941:114:22FVMWLT1:2:2162:13240:14963/151‑1 (MQ=60)
                                                  atcttgtctcgtgggctcggagatgtgtataagagacagCAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCC                                                                                                       <  LH00941:114:22FVMWLT1:2:1147:29955:4555/112‑1 (MQ=60)
                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCC                                                                                                      <  LH00941:114:22FVMWLT1:2:2155:36741:8718/151‑1 (MQ=60)
                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCC                                                                                                      <  LH00941:114:22FVMWLT1:2:2134:13592:9487/151‑1 (MQ=60)
                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCC                                                                                                      <  LH00941:114:22FVMWLT1:2:1135:23928:27261/151‑1 (MQ=60)
                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCC                                                                                                      <  LH00941:114:22FVMWLT1:2:2254:16938:25468/151‑1 (MQ=60)
                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCC                                                                                                      <  LH00941:114:22FVMWLT1:2:2247:6140:16436/151‑1 (MQ=60)
                                                   GGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCC                                                                                                      <  LH00941:114:22FVMWLT1:2:2104:7370:15363/151‑1 (MQ=60)
                                                      GGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCcag                                                                                                   <  LH00941:114:22FVMWLT1:2:1147:11031:24651/151‑4 (MQ=60)
                                                      GGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTT                                                                                                   <  LH00941:114:22FVMWLT1:2:1109:32063:17589/151‑1 (MQ=60)
                                                                   TCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCcaggtttgttgcatac                                                                                      <  LH00941:114:22FVMWLT1:2:2245:28541:9535/151‑17 (MQ=60)
                                                                    CCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCACA‑‑‑CA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAACTTCCACGTGTATGCAACAAACCT‑GGGGCTGCTACACAACCCTTTCGCGACACAAGGCC                                                                                     >  LH00941:114:22FVMWLT1:2:2257:47419:28942/1‑151 (MQ=60)
                                                                          GTCGTGTGTCTCCGACAGCAGAACAACACCACTCGGGTGGGGGAGTGGCTTTGTGTAGCGAAGGGGCCGCA‑‑‑TA‑‑‑GTGTCC‑‑‑CC‑‑‑GGCATTTTCTGTGTGTAGAAAAAAAACTT‑GGGGTTGCTAGGCAGCCCTTTCGCGACACAAGGCCCACAAA                                                                               <  LH00941:114:22FVMWLT1:2:2157:28578:23114/151‑1 (MQ=9)
                                                                          GTCATTTGCCGCCGACAGCATATCAACCCCGCTCAGGTGGGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATGGTG‑‑‑G‑‑‑CC‑‑‑CC‑‑‑GGCAATTTCCGCGTGTATGCAAAAAACTT‑GGGGCTGCGAGGCAGCCCTTTCGAGACACAAGGCCCACAAA                                                                               <  LH00941:114:22FVMWLT1:2:2229:23678:19143/151‑1 (MQ=60)
                                                                           TCATGTGTCGCCGACAGCAGAGAAACACCGTTCGGGTGGGGGAGTGGCTTTGTTTCGCGAAGGGGCCGCA‑‑‑TAGTGG‑‑‑CC‑‑‑CT‑‑‑GGCAGTTTCCGTGTGTATGCAACAAACTT‑GGGGTTGTTATGCATCCCTTTCGCGACACAAGGCCCACAAAG                                                                              <  LH00941:114:22FVMWLT1:2:2256:47253:10047/151‑1 (MQ=43)
                                                                            cattttcCGCCGACAACATAGCAACACCACTCAGGTGGGGGAGCGCCCTTGTGTCGCGAAAGGGCCGCA‑‑‑GA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTCCAGTGTGTATGCAACAAACCT‑GGGGTTGCTATGCATCCCTTTCGCGACACAAGGCCCACAAAGG                                                                             <  LH00941:114:22FVMWLT1:2:1242:4550:15508/144‑1 (MQ=60)
                                                                            CCTGTGTCGCAGACAGCATGGCAACACCGTTCAGGGGTGGGAGTGGCCTTGTGTCGCGAAAGTGCCGCG‑‑‑TA‑‑‑G‑‑‑CGTCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAACCCT‑GGGGTTACTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGG                                                                             <  LH00941:114:22FVMWLT1:2:1149:6455:28926/151‑1 (MQ=60)
                                                                            CATGTTTCGCCGAAAGCATATCAACACCTCTCAGGTGGGGGAGCGGCTTTGTGTCGCGAAAGGGCCGCA‑‑‑GA‑‑‑GTGTCC‑‑‑CC‑‑‑GGCAGTTTCCGTGTGTATGCAACAAACCT‑GGGGCTGCTATGAAGCCCTTTCGCGACACAAGGCCCACAAAGG                                                                             <  LH00941:114:22FVMWLT1:2:1120:40384:10512/151‑1 (MQ=60)
                                                                            CATGTGTCACCGACAGAATAGCAACACCGCTGAGGTGGGGGAGGGGCTTTGTGTCGCGAAGGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAGTTTCAGCGGGGATGAAAAAAACTT‑GGGGTTGCTATGCACCCCTTTCGCGACACAAGGCCCACAAAGG                                                                             <  LH00941:114:22FVMWLT1:2:1140:42057:6877/151‑1 (MQ=47)
                                                                            AATGTGTCGCCGACAACACAGAAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCG‑‑‑GGCAATTTCCGTGTGTATGAAAAAAACCT‑GGGGCTGCTATGCATCCCTTTCGCGACACAAGGCCCACAAAGG                                                                             <  LH00941:114:22FVMWLT1:2:1139:35456:17669/151‑1 (MQ=60)
                                                                              TGTGTTTCCGACAGCATAGCAACACCTCTCATGTGTGGGAACGGCCTTGTGTCGCGAAAGGCCCGCA‑‑‑TA‑‑‑G‑‑‑CGTCCCC‑‑‑GGAAATTTCCGCTTGTATGCACCAAACCT‑TGGGTTTCTCTACAACCCTTTCGCGACACAAGGCCCACAAAGGAC                                                                           <  LH00941:114:22FVMWLT1:2:2121:19804:5612/151‑1 (MQ=56)
                                                                              TGTGTCGCCGACAGCATAGCAACCCCTCTCGGGTGTGGGAGTGGCCTTGTGTCGCGAAAGGGCCGAA‑‑‑GA‑‑‑GTGTCC‑‑‑CT‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGAC                                                                           <  LH00941:114:22FVMWLT1:2:2226:43536:17733/151‑1 (MQ=60)
                                                                                        ACAGCATAGAAACACCGCTAAGGTGTGGGAGTGGCCTTGTTTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑TG‑‑‑CCCCTGGCACTTTCCGCGGGGAGGCAAAAACCCT‑GGGGCTACGATGAAGCCCTTTCGAGACACAAGGCCCACAAAGGACCGCGCAGGCT                                                                 <  LH00941:114:22FVMWLT1:2:1257:34781:21240/151‑1 (MQ=59)
                                                                                             agagcaacacctctaggggggGGGAGCGGCTTTGTGTCGAGAAGGGGCCGCA‑‑‑GA‑‑‑G‑‑‑CGACCCC‑‑‑GGCAGTTTCCGGGTGTATAAAACAAACTT‑GGGGCTGCTATGAAGCCTTTTCGCAACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGG                                                            <  LH00941:114:22FVMWLT1:2:2237:49259:24283/130‑1 (MQ=56)
                                                                                             ATAGCAACACCCCTCAGGTGTGGGAATGGCCTGGTTTCTCGAAAGGGCCGAA‑‑‑TA‑‑‑G‑‑‑CGCCCCC‑‑‑GACAGTTCCCGCGTGTATGAAACAAACCT‑GGGGCTGCTATGCATCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGG                                                            <  LH00941:114:22FVMWLT1:2:2158:47678:25067/151‑1 (MQ=60)
                                                                                               agcaacccctctcgggtgggggagtggctttgtttcgcaaagggtCCGCA‑‑‑TA‑‑‑G‑‑‑CGTCCCC‑‑‑GGCAATTTCCGCGTGTATGCAAAAACCCT‑GGGGTTGCTATACATCCCTTTCGCAACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC                                                          <  LH00941:114:22FVMWLT1:2:2132:23881:1176/106‑1 (MQ=60)
                                                                                                  AACACCTCTCAGGTGGGGGAGCGGCCTTGTTTCGCGAAGGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACTT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGG                                                       <  LH00941:114:22FVMWLT1:2:2216:18982:23114/151‑1 (MQ=60)
                                                                                                  AACACCGTTCGGGGGTGGGAGTGGCTTTGTGTCGGGAAAGGGCTGCA‑‑‑GA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGGGGGCAACAAATTG‑GGGGTTGCGATGCAGCCCTTTCGTGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGG                                                       <  LH00941:114:22FVMWLT1:2:2216:19000:23114/151‑1 (MQ=40)
                                                                                                     ACCGCTCGGGTGTGGGAGCGGCTTTGTGTCGGGAAGGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGTGTGTATGAAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAAC                                                    <  LH00941:114:22FVMWLT1:2:1267:3589:16693/151‑1 (MQ=60)
                                                                                                          taggtggggggggaggccgTGTGTAGCGAAAGGGCCGCA‑‑‑TG‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAGTTCCCGCGTGTGTGCAAAAAACCT‑GGGGCTGCGATGCAGCCCTGTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCC                                               <  LH00941:114:22FVMWLT1:2:2257:38692:9823/132‑1 (MQ=60)
                                                                                                          CCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑CA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTCCTGCGGGTATACAA‑AAACCTGGGGGTTGCTATGCAGCCTTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCC                                               <  LH00941:114:22FVMWLT1:2:2218:40550:17781/151‑1 (MQ=60)
                                                                                                          CCAGGTGGGGGAGCGGCTTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTCCCGCGTGTGTGAAAAAAACCT‑GGGGTGGCTGTGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCC                                               <  LH00941:114:22FVMWLT1:2:1131:40264:1881/151‑1 (MQ=60)
                                                                                                               TGTGGGAGTGGCTTTGTTTTGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAGTTTCCGTGGGTATGCAACAAACCG‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGC                                          <  LH00941:114:22FVMWLT1:2:2263:5410:14179/151‑1 (MQ=60)
                                                                                                               TGTGGGAGTGGCCTTGTGTCGTGAAGGGGCCGCA‑‑‑TG‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAGTTTCCGCGTGTATGAAACAAACCT‑GGGGTTACGATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGC                                          <  LH00941:114:22FVMWLT1:2:1264:9015:13922/151‑1 (MQ=60)
                                                                                                               TGTGGGAGCGGCCTTGTGTTGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGGGTATACAACAAACCT‑GGGGCGGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGC                                          <  LH00941:114:22FVMWLT1:2:2149:46892:22649/151‑1 (MQ=60)
                                                                                                               TGTGGGAGCGGCCTTGTGTCGCAAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGTCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGAAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGC                                          <  LH00941:114:22FVMWLT1:2:1264:9025:13938/151‑1 (MQ=60)
                                                                                                               TGGGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCT‑‑‑GGCAATTTCCGCGTGTATGCAACAAACTT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGC                                          <  LH00941:114:22FVMWLT1:2:2263:5428:14179/151‑1 (MQ=60)
                                                                                                                TTGGGAGCGGCCTGGTGTAGCGAAAGGGCCGCA‑‑‑GA‑‑‑G‑‑‑CGGCCCT‑‑‑GGCAATTTCCTCGTGTATGCAAAAAACTT‑GGGGTTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCT                                         <  LH00941:114:22FVMWLT1:2:1255:14267:11008/151‑1 (MQ=60)
                                                                                                                    ggagatgtgtataagagacagGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCG                                     <  LH00941:114:22FVMWLT1:2:1246:26710:20007/130‑1 (MQ=60)
                                                                                                                    GGGCGGCCGTGTGTCGCGAAAGGGCCACA‑‑‑TAGCTG‑‑‑CC‑‑‑CC‑‑‑GACAATTTCCGCGTGTGTGAAACAAACCG‑GGTGCTGTTATGCAGCCTTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCG                                     <  LH00941:114:22FVMWLT1:2:2226:22180:23658/151‑1 (MQ=60)
                                                                                                                              GTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAG                           >  LH00941:114:22FVMWLT1:2:1156:19777:26733/1‑151 (MQ=60)
                                                                                                                                    CGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCA                     >  LH00941:114:22FVMWLT1:2:1109:31203:2457/1‑151 (MQ=60)
                                                                                                                                    CGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCA                     >  LH00941:114:22FVMWLT1:2:1125:8535:13954/1‑151 (MQ=60)
                                                                                                                                         GGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAActgtctcttatacacatctccgagcccacgagacaagatacacgatctgggggggggggggtgg                >  LH00941:114:22FVMWLT1:2:2221:40273:21048/1‑87 (MQ=60)
                                                                                                                                         GGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCgcggattcgctgtagtggccgatgaggtgcg                >  LH00941:114:22FVMWLT1:2:2140:36445:28990/1‑120 (MQ=60)
                                                                                                                                         GGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCgcggattcgctgtagtggccgatgaggtgcg                >  LH00941:114:22FVMWLT1:2:1239:20257:18438/1‑120 (MQ=60)
                                                                                                                                         GGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCG                >  LH00941:114:22FVMWLT1:2:1104:37675:21320/1‑151 (MQ=60)
                                                                                                                                         GGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCA                >  LH00941:114:22FVMWLT1:2:1269:9025:29311/1‑151 (MQ=60)
                                                                                                                                         GGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCA                >  LH00941:114:22FVMWLT1:2:1159:18565:23706/1‑151 (MQ=60)
                                                                                                                                         GGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCA                >  LH00941:114:22FVMWLT1:2:1148:47576:4779/1‑151 (MQ=60)
                                                                                                                                         GGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCA                >  LH00941:114:22FVMWLT1:2:1112:36667:2890/1‑151 (MQ=60)
                                                                                                                                         GGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCA                >  LH00941:114:22FVMWLT1:2:1203:41151:9983/1‑151 (MQ=60)
                                                                                                                                         GGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCA                >  LH00941:114:22FVMWLT1:2:2240:8146:15972/1‑151 (MQ=60)
                                                                                                                                         GGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCA                >  LH00941:114:22FVMWLT1:2:1230:25934:14531/1‑151 (MQ=60)
                                                                                                                                         GGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCA                >  LH00941:114:22FVMWLT1:2:2112:39459:20792/1‑151 (MQ=60)
                                                                                                                                         GGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCA                >  LH00941:114:22FVMWLT1:2:1159:18556:23690/1‑151 (MQ=60)
                                                                                                                                         GGGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCA                >  LH00941:114:22FVMWLT1:2:2129:15071:8590/1‑151 (MQ=60)
                                                                                                                                          GGCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCC                                                                                     >  LH00941:114:22FVMWLT1:2:2205:29835:16036/1‑81 (MQ=60)
                                                                                                                                           GCCGCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATC              >  LH00941:114:22FVMWLT1:2:2251:22578:10576/1‑151 (MQ=60)
                                                                                                                                              GCA‑‑‑TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAG           >  LH00941:114:22FVMWLT1:2:2141:46809:10528/1‑151 (MQ=60)
                                                                                                                                                    TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAGTTTCCGCGTGTATACAACAACCTT‑GGGGCTGCTATGCACCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCAC        <  LH00941:114:22FVMWLT1:2:1223:1545:28750/151‑1 (MQ=60)
                                                                                                                                                    TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCAC        >  LH00941:114:22FVMWLT1:2:2108:23317:2345/1‑151 (MQ=60)
                                                                                                                                                    TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCAC        >  LH00941:114:22FVMWLT1:2:1250:26146:27581/1‑151 (MQ=60)
                                                                                                                                                    TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCAC        >  LH00941:114:22FVMWLT1:2:1225:19065:4395/1‑151 (MQ=60)
                                                                                                                                                    TA‑‑‑G‑‑‑CGGCCCC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCAC        >  LH00941:114:22FVMWLT1:2:2130:20396:26396/1‑151 (MQ=60)
                                                                                                                                                         G‑‑‑CC‑‑‑CC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCtgtctcttatacacatctccgagcccacgagacaagatacacgatctggggggg   >  LH00941:114:22FVMWLT1:2:1202:33228:14867/1‑97 (MQ=60)
                                                                                                                                                         G‑‑‑CC‑‑‑CC‑‑‑GGCAATTTCCGCGTGTATGCAAAAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCtgtctcttatacacatctccgagcccacgagacaagatacacgatctggggggg   >  LH00941:114:22FVMWLT1:2:1202:33247:14867/1‑97 (MQ=60)
                                                                                                                                                             CC‑‑‑CC‑‑‑GGCAATTTCCGAGTGTAGGCAACAACCTT‑GGGGCTGCTATGCAGCCCTTTCCCAACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCTTGCT  <  LH00941:114:22FVMWLT1:2:1143:26738:21977/151‑1 (MQ=60)
                                                                                                                                                              |                                                                                                                                                            
TACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCA‑‑‑TA‑‑‑G‑‑‑CG‑‑‑CC‑‑‑GGCAATTTCCGCGTGTATGCAACAAACCT‑GGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCTTGCT  >  AE015451_phaM1/5555459‑5555757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑