Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F34 I0 R1
|
25 |
22.0 |
717516 |
96.1% |
689532 |
148.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
CP009273 |
2,718,735 |
G→T |
54.7% |
P124Q (CCG→CAG) |
kgtP ← |
alpha‑ketoglutarate transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP009273 | 2,718,735 | 0 | G | T | 54.7%
| 7.9
/ 10.8
| 19 | P124Q (CCG→CAG) | kgtP | alpha‑ketoglutarate transporter |
| Reads supporting (aligned to +/- strand): ref base G (5/4); new base T (5/5); total (10/9) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCG‑CCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACC > CP009273/2718594‑2718868
|
ccgATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCG‑CCAACAGATAATCCCTGAAATAAACGAGCGAGAACCAATAATGCCTGAGCCCa > 2:280735/1‑149 (MQ=255)
tCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCG‑CCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCACTAATGCCGGAGCCCACGTa > 2:224536/1‑149 (MQ=255)
aaCGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCG‑CCAACAGATAACACCTTAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTAcc > 2:243287/1‑149 (MQ=255)
caTACCGAAATGATGCGTAAAAACCTATGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCCCCG‑CCAACAGATAATCCCTGATATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGtt < 1:259222/149‑1 (MQ=255)
gCGTAAAAACCTTGGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCG‑CCAACCGATAATCCCTGAAATAAAAGAGCGAGAAGCAATAATACCGGAGCCCACGGACCTACAAGTTCATAACCCGagag > 1:916/1‑149 (MQ=255)
aaaaCCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCG‑CCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGaggcagg > 2:285024/1‑149 (MQ=255)
aCCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCG‑CCAACAGATAATCCCAGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCTCGTACCTATAGTTTCATAACCTGGGAGGCAGGCGa < 1:215311/149‑1 (MQ=255)
cgcCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGAATATGAGA‑AAGCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAg < 2:171858/149‑1 (MQ=255)
cgcCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCG‑CCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAg > 1:70146/1‑149 (MQ=255)
cAACGGCAACTTCCCTCAGATAGGTGGCGCTGGTGCCATATCCTCCG‑CCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCg < 1:316630/149‑1 (MQ=255)
cGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCG‑CCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGaaa < 2:160821/149‑1 (MQ=255)
gTGGCGCTGGTGCCATATTCTCCG‑CCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGGTaa > 1:333494/1‑149 (MQ=255)
cATATTCTCCG‑CCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAACCACATCATACACACCGATAACAGCATCGAtttt > 1:125067/1‑149 (MQ=255)
atgctcCA‑CCAACAGATAATCCCTGAAATAACCGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGCAGGAAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTg < 2:143976/146‑1 (MQ=255)
aGATAATCCCTGAAACAAACGGGCGAGAAGCAATAATGCCGGCGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTAt < 1:151206/149‑1 (MQ=255)
ccTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATg > 1:43024/1‑149 (MQ=255)
gAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATAGGCTATGCgg > 1:358105/1‑149 (MQ=255)
aTAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCa < 2:32265/149‑1 (MQ=255)
agaAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAAcc < 2:290253/149‑1 (MQ=255)
|
CCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCG‑CCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACC > CP009273/2718594‑2718868
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
