Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F34 I40 R1
|
11 |
55.0 |
1783796 |
96.5% |
1721363 |
148.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
CP009273 |
2,718,735 |
G→T |
P124Q (CCG→CAG) |
kgtP ← |
alpha‑ketoglutarate transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP009273 | 2,718,735 | 0 | G | T | 98.1%
| 160.9
/ NA
| 53 | P124Q (CCG→CAG) | kgtP | alpha‑ketoglutarate transporter |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (22/29); total (23/30) |
| Fisher's exact test for biased strand distribution p-value = 4.42e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGC > CP009273/2718590‑2718879
|
tCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAAATTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGCGCGAGAAGCAATAATGCCTGAg > 2:299962/1‑149 (MQ=255)
cgccgATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGcc > 2:729107/1‑149 (MQ=255)
gccgATCAACGTCACATACTGAAATGATGCGTAAAAACCTATGCGCCCTTCAACGAGAATGGCACGCAGATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAACCGGGCGAGAAGCAATAATGCCGGGGcgc > 2:350637/1‑147 (MQ=255)
aTCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCCCGt > 1:672952/1‑149 (MQ=255)
aCGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCt > 2:179703/1‑149 (MQ=255)
gTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCtat < 1:868278/149‑1 (MQ=255)
tACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTc < 1:291632/149‑1 (MQ=255)
tACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTc > 1:642426/1‑149 (MQ=255)
tGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGGGCGAGAGGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATa > 1:685692/1‑149 (MQ=255)
gAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATaa < 1:403857/149‑1 (MQ=255)
tgatgCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTg < 1:501211/149‑1 (MQ=255)
tgCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCACTGGTGCCATATTCTCCGCCAACAGAGAATCCCTGAGATAAACGCGCGAGAAGCAATATGGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGa < 2:128417/149‑1 (MQ=255)
gCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGag < 1:277741/149‑1 (MQ=255)
gTAAAAGCCGTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGaggc < 1:734775/149‑1 (MQ=255)
tAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGAaggca > 2:792799/1‑149 (MQ=255)
aaaaaCCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCGATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATGGTTTCATAACCTggagagcag > 1:463764/1‑142 (MQ=255)
aaatCCTTTGCGCCCTTCAACGGAACCGTCCCTCATATAGGTGGCGCTGGTGCCATATTCTCCGGCAACAGATAATCCCTGAAATAACCGATCGAGAAGCCATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGaggcagg < 1:391010/149‑1 (MQ=255)
acaCCTTTGCGCCCTTCAACGGCAACTTCACTAATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGaggcaggc < 1:295562/147‑1 (MQ=255)
cgcCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAg < 1:465284/149‑1 (MQ=255)
ccTTCAACGGCAACTTCACTCATATAGGGGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATACACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGa < 1:435672/149‑1 (MQ=255)
cTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGAt > 2:7600/1‑149 (MQ=255)
aaCGCCAACTTCACTCATATAGGGGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGa < 1:19527/149‑1 (MQ=255)
aCGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGaa < 2:154698/149‑1 (MQ=255)
aCTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGAGACCCGCGacacct > 1:841123/1‑147 (MQ=255)
ttCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACAcatca < 1:7600/149‑1 (MQ=255)
cTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCCACAGATAATCCCAGAAATAAACGCGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATaca < 2:412026/149‑1 (MQ=255)
tCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGCTCCGAAACACATCATacac > 2:100145/1‑149 (MQ=255)
atatAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATacacac < 1:690868/149‑1 (MQ=255)
ataGGTGGCGCTTGTGCCATATTCTGCGCCAACAGAGAGTCCCTGAAATAAACGAGCGAGAAGCAATACTGCCCGCGCCCACGTACCTATATTTTCATAACCTGGTAGGCAGGCGATAACCAGCGATCCGAAACACATCATACTCACCg < 2:528686/149‑1 (MQ=255)
aGGTCGCGCTGGTGCCATATTCCCCGCCAACACATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGAt < 2:328399/149‑1 (MQ=255)
gTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAACCACATCATACACACCGATac > 2:891798/1‑148 (MQ=255)
gTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATaa > 2:673752/1‑149 (MQ=255)
cgcTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGc > 1:886832/1‑149 (MQ=255)
gcTGGTGCCATATTCTCCGCCAACAGATAAACCCAGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAGCACATCATACACACCGATAACAGCa < 1:766494/149‑1 (MQ=255)
cTGGTCCCATATGCGCCGCCAACGTATAATCCCTGAAATAAACGAGCGACAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCAt < 1:77001/149‑1 (MQ=255)
ggTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAGACACATCATACACACCGATAACAGCATCg < 2:543619/149‑1 (MQ=255)
cATATTCTCCGCCGACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGAtttt > 1:544526/1‑149 (MQ=255)
tatTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGATTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGCTAACAGCGTCGCtttttt > 1:255346/1‑149 (MQ=255)
atTCGCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAAGGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTg < 1:484202/149‑1 (MQ=255)
ttCTCCGCCAACAGATAAGCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGc < 2:57281/149‑1 (MQ=255)
tcCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGAc > 1:557759/1‑149 (MQ=255)
gCCAACAGATAATCCCTGAAATAAACGGGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGTCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCAt < 2:393095/149‑1 (MQ=255)
gCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAACCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAGCAGCATCGATTTTTTGCGACCAt < 1:612799/149‑1 (MQ=255)
gCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGAAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACAAGCGATCCGAAACACATCATACACACCGATAACAGCATCGGTTTTTTGCGACCAt > 1:250649/1‑149 (MQ=255)
ccTGAAATAAACGAGCGAGAAGCGATAACGCCTGAGCCCACGTACCTATAGTTACATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATg > 2:493155/1‑149 (MQ=255)
cTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGAACCGAAACACATCATACACACCGATAACAGCATAGCTTTTTTGCGACCATGTTTATCGGCTATGc > 2:323650/1‑149 (MQ=255)
aTAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTCCCTATAGTGTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCa > 2:880005/1‑149 (MQ=255)
ggcgggAATCAATAATGCCTGAGGCGCCGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGcc < 1:85807/148‑1 (MQ=255)
gaAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCg > 1:876988/1‑149 (MQ=255)
aaGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTGGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGc < 2:332208/149‑1 (MQ=255)
gaaataacGCCTGAGCCCCCGTACCTAGAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCt < 2:241195/147‑1 (MQ=255)
ataatGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACATCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTAtt < 1:79058/149‑1 (MQ=255)
tGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGgc < 2:488677/149‑1 (MQ=255)
|
TCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGC > CP009273/2718590‑2718879
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
