Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A16 F20 I40 R1	12	45.4	1473824	96.3%	1419292	148.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	3,019,631	G→T	M169I (ATG→ATT)	guaD →	guanine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,019,631	0	G	T	100.0%	108.0 / NA	38	M169I (ATG→ATT)	guaD	guanine deaminase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (13/25); total (13/25)

ATGTCGGCGTTCTTCATCAAGCAGCTTTTACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACGCTTCGCCCCGACCTCATCTCCTGAAC  >  CP009273/3019488‑3019776

ttGTCGGCGGTCTTCTTCAAGCAGCTTTTACGTAACCGAACCACCACGGTGCTGGGGGTTGGCACTGTTCATCCGCAATCTTTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGa                                                                                                                                              <  2:356357/148‑1 (MQ=255)

 gtgcGGCGTTCGTCTTCCAGCAGGTTTGACGTAACGGAACGCCCACGGCGCGGGTGTTTGTCACTTGTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGAc                                                                                                                                             <  2:117144/146‑1 (MQ=255)

   tCGGCGTTCTTCATCAAGCAGCTTTTACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCg                                                                                                                                           <  2:60168/149‑1 (MQ=255)

       cGTTCTTCATTAAGCAGCTTTTACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAAc                                                                                                                                       <  1:218117/149‑1 (MQ=255)

               atcaAGCAGCTTTTACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGa                                                                                                                               >  2:684182/1‑149 (MQ=255)

                tcaAGCAGCTTTTACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTAATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTGTGGACCGCAACGCACCGGag                                                                                                                              >  1:103571/1‑148 (MQ=255)

                   agcagcTTTTACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTAt                                                                                                                           >  2:540881/1‑149 (MQ=255)

                         ttttACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGCGATTATGAACCGCAACGCACCGGATTATCTGCTc                                                                                                                     >  2:476034/1‑149 (MQ=255)

                                  aCGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGcc                                                                                                            >  1:218865/1‑149 (MQ=255)

                                                     ggTGTTGGGCACTGTTCATCCGCAATCTGTGTCTGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGGATGATTGCTGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTACTCGACACTGCCGAAAGCAGCTATCACccaa                                                                                         <  2:348866/149‑1 (MQ=255)

                                                      ggggttGGCACTGTTCATCCCCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACcaaag                                                                                        <  2:11261/145‑1 (MQ=255)

                                                       tgtTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACcaaagc                                                                                       <  2:218865/149‑1 (MQ=255)

                                                          ttGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACcaaagcaaa                                                                                    <  1:585091/149‑1 (MQ=255)

                                                              cACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAAc                                                                                <  2:672793/149‑1 (MQ=255)

                                                                cTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATCTCAATATACGTATGATTGCCGGTCAAGCGATTATGGACCGCAACGCACCGGATTATCTGCCCGAGACTACCCAAAACAGCCAGCCCCAAAGCCAACAACTg                                                                              >  2:694549/1‑149 (MQ=255)

                                                                  ggtCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGAt                                                                            <  1:68429/147‑1 (MQ=255)

                                                                  gTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGAt                                                                            <  2:560032/149‑1 (MQ=255)

                                                                  gTTCATACGCGATCTGTTGATGCGATGTTTGAAGACGCCAGTCATATCAAAATGCGTATGATAGCCGGTAAGGTGCTTATGGACCGCAACGCAACGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGAt                                                                            <  2:586897/149‑1 (MQ=255)

                                                                       cccGCAATCTGTTGATGCGCATTTTGAAACAGCCAGTCATATCACTATGCAAATGATTGCGGGTAAGGTGGTTATGGACCGCAACGCACCGGATTATGTTCTCGGCACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAAc                                                                       <  2:614105/148‑1 (MQ=255)

                                                                         cGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGc                                                                     >  1:519082/1‑149 (MQ=255)

                                                                           cAATCTGTTGATGCTCTGTTTGAAGCCGCCAGGCATATCACTATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCGCGACACTGCGGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTg                                                                   <  2:362862/149‑1 (MQ=255)

                                                                                    gATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCCATCACCAAAGCAAAGAACTGCACGAACGCTGGAACaaaaa                                                          >  2:652777/1‑149 (MQ=255)

                                                                                     aTGCGCTGTTTGAAGCCGCCAGCCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAAt                                                         <  1:283912/149‑1 (MQ=255)

                                                                                       gcgcTGTTTGAAGCCGCCAGTCATATCAATATGCGGATGATTGCCGGGAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACCCTGCCGAAAGCAGCTATCACCAAAGCAAAGAACATATCGAACGCTGCCACAAAAAAgg                                                       >  2:610849/1‑149 (MQ=255)

                                                                                         gcTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGgtc                                                     <  2:731740/149‑1 (MQ=255)

                                                                                         gcTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGgtc                                                     <  2:130525/149‑1 (MQ=255)

                                                                                            gTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTACCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATGGAACGCTGGCACAAAAATGgtcggc                                                  >  1:469474/1‑147 (MQ=255)

                                                                                                gAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCt                                              <  1:382834/149‑1 (MQ=255)

                                                                                                gAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCt                                              <  2:714243/149‑1 (MQ=255)

                                                                                                     cgccAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATg                                         <  2:293456/149‑1 (MQ=255)

                                                                                                      gccAGTCTTATCAATATGCGTATGATTGCCGGTAAGGTGAGTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGc                                        <  2:620715/149‑1 (MQ=255)

                                                                                                             atatCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCAAAGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAGAACAGGTCGTCTGCTATATGCGATTACg                                 >  2:701408/1‑149 (MQ=255)

                                                                                                                tCAATATGCGTATGACTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCa                              <  1:68484/149‑1 (MQ=255)

                                                                                                                tCAATACGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCCGCTATCACCAAAGCAAAGACCTGAGCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCa                              <  1:305362/149‑1 (MQ=255)

                                                                                                                     atGCGTATGATTGCCGCTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACGCtt                         <  1:576865/149‑1 (MQ=255)

                                                                                                                               ttGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACGCTTCGCCCCGAcc               <  2:559207/149‑1 (MQ=255)

                                                                                                                                  ccGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACGCTTCGCCCCGACCTCa            >  1:140376/1‑149 (MQ=255)

                                                                                                                                          gTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACGCTTCGCCCCGCCCTCATCTCCtta    >  2:197260/1‑147 (MQ=255)

                                                                                                                                           tgattaTGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCCCCCACCGGCGGTTCCACCATATGCGAGTACGCCACGCTTCGCCCCGACCTCATCTCCTGaa   >  2:519694/1‑149 (MQ=255)

                                                                                                                                            gattatGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACGCTTCGCCCCGACCTCATCTCCTGAAc  <  2:467817/149‑1 (MQ=255)

ATGTCGGCGTTCTTCATCAAGCAGCTTTTACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACGCTTCGCCCCGACCTCATCTCCTGAAC  >  CP009273/3019488‑3019776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A