Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F121 I0 R1	43	32.8	695000	96.9%	673455	140.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,474,639	C→G	100%	A9129P (GCA→CCA)	ebh ←	extracellular matrix binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,474,639	0	C	G	100.0%	127.4 / NA	39	A9129P (GCA→CCA)	ebh	extracellular matrix binding protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (20/19); total (20/19)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGCGACCTTTAAATCCATCTTGTTGAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGCTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGTTTGCTCAACGTTAGCA  >  CP000730/1474501‑1474766

tGCGACCTTTAAATCCATCTTGTTGAGCATTGTTTAATGCATTAAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGtt                                                                                                                               >  2:315754/1‑141 (MQ=255)

   gACCTTTAAATCCATCTTGTTGAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGAt                                                                                                                            <  2:185132/141‑1 (MQ=255)

    aCCTTTAAATCCATCTTGTTGAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATc                                                                                                                           >  2:81368/1‑141 (MQ=255)

    aCCTTTAAATCCATCTTGTTGAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATc                                                                                                                           >  2:341004/1‑141 (MQ=255)

       tttAAATCCATCTTGTTGAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAACAAATTTTGAAGTGGTTGATCCAc                                                                                                                        >  2:53389/1‑141 (MQ=255)

           aaTCCATCTTGTTGAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACttgt                                                                                                                    <  1:261717/141‑1 (MQ=255)

                   ttgttgCGTATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTGAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTAtt                                                                                                            <  2:167251/141‑1 (MQ=255)

                     gttgAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGCTTACCATTTGTTTTCGCAAGTGTTCCAGCATTATCACGCTTTAACGGTAGTTGTGGGTTTAAATTAATTTGGAGTGGTTGATCCACTTGTTGCTTAttag                                                                                                          >  2:287842/1‑139 (MQ=255)

                      ttgAGTATTGTTTCATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTTGCTAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGc                                                                                                         <  2:227291/141‑1 (MQ=255)

                      ttgAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGc                                                                                                         <  1:3695/141‑1 (MQ=255)

                        gAGCTTTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCAt                                                                                                       >  2:238739/1‑141 (MQ=255)

                         aGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCAtt                                                                                                      <  1:299166/141‑1 (MQ=255)

                                tttAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAACTATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTc                                                                                               <  1:272180/141‑1 (MQ=255)

                                 ttAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCa                                                                                              >  1:136563/1‑141 (MQ=255)

                                      gCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATAcc                                                                                         >  2:244291/1‑140 (MQ=255)

                                        aTTCGATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACAtt                                                                                       <  1:108163/141‑1 (MQ=255)

                                            aaTTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTtca                                                                                   >  1:9256/1‑141 (MQ=255)

                                            aaTTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTtca                                                                                   >  1:42802/1‑141 (MQ=255)

                                               tGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTtcagca                                                                                >  2:339775/1‑141 (MQ=255)

                                                  tCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTtcagcatca                                                                             >  1:330552/1‑141 (MQ=255)

                                                   cGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTtcagcatcac                                                                            >  2:272465/1‑140 (MQ=255)

                                                          aTGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTACAATTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGc                                                                     >  1:217184/1‑141 (MQ=255)

                                                           tGTTTACCATTTTTTTTAGCATGTGCTACACGTTCATCACCATTTAATGCTTGTTGTGCTTTTAAAATATTTTGAAGTGGTTGATCCTCTTGTTTCTTATTCGCATTTTTTTTCAATACATTTTCAGCATCAGCAATAGCt                                                                    <  2:131703/141‑1 (MQ=255)

                                                           tGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCCTGTTTTGCACTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATTAGCAATAGCt                                                                    <  2:169986/141‑1 (MQ=255)

                                                                 ccATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAGTATTTTGAAGTGGTTGATCCACTTGTTGCTAATTCGCCTTGGGATTGAATAGATTTTCTGCATCAGCAATAGCTTGtttt                                                              >  2:155251/1‑140 (MQ=255)

                                                                     ttGTTTTAGCTAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAg                                                          <  2:165439/141‑1 (MQ=255)

                                                                           tAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATATAGCAGTGGTTGATCCACTTGTTGCTTGTTCGCATTTTGTTTCAATACGTTTTCAGCATCCGCAATAGCTTGTTTATAAGCAtttt                                                    >  1:149262/1‑141 (MQ=255)

                                                                            aGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTg                                                   >  1:279125/1‑141 (MQ=255)

                                                                                    cTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAGTATCGTCAGGCGGGTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTg                                           <  1:111587/141‑1 (MQ=255)

                                                                                              atcaCCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTtg                                 <  1:81368/141‑1 (MQ=255)

                                                                                              atcaCCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTtg                                 <  1:341004/141‑1 (MQ=255)

                                                                                                caCCATTTAATGCTTGTTTTGCATTTAAAATATTTTGACGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTtgtg                               >  2:252854/1‑141 (MQ=255)

                                                                                                caCCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTCTTCGCATTTTGTTTCAATACATTTTCAGCATCGGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTggtt                               >  2:224932/1‑140 (MQ=255)

                                                                                                caCCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTtgtg                               <  1:315754/141‑1 (MQ=255)

                                                                                                          tggttGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTGCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTAc                     <  2:10082/138‑1 (MQ=255)

                                                                                                              ttttttTCATTTAAAATATTTTTATTTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGtt                 <  2:150495/139‑1 (MQ=255)

                                                                                                                   tGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGTTTGCTc            <  1:142811/141‑1 (MQ=255)

                                                                                                                            aaTATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGTTTGCTCAACGTTAGc   <  1:300869/141‑1 (MQ=255)

                                                                                                                             atatTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGTTTGCTCAACGTTAGCa  >  2:328467/1‑141 (MQ=255)

TGCGACCTTTAAATCCATCTTGTTGAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGCTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGTTTGCTCAACGTTAGCA  >  CP000730/1474501‑1474766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A