breseq  version 0.32.1  revision 5f2cb669173e
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 37,746 A→T 30.5% intergenic (+442/‑67) USA300HOU_0028 → / → ugpQ1 hypothetical protein/glycerophosphodiester phosphodiesterase
RA 39,246 C→T 50.0% A630T (GCT→ACT)  mecA ← penicillin binding protein 2 prime
RA 39,378 C→A 43.3% D586Y (GAT→TAT)  mecA ← penicillin binding protein 2 prime
RA 71,870 T→C 8.9% A368A (GCA→GCG arcD1 ← arginine/ornithine APC family amino acid‑polyamine‑organocation transporter, antiporter
RA 71,990 A→G 9.1% N328N (AAT→AAC arcD1 ← arginine/ornithine APC family amino acid‑polyamine‑organocation transporter, antiporter
RA 110,651 C→T 10.6% D170D (GAC→GAT USA300HOU_0109 → staphylococcal tandem lipoprotein
RA 110,660 T→A 15.0% I173I (ATT→ATA USA300HOU_0109 → staphylococcal tandem lipoprotein
RA 111,512 A→G 27.4% S194S (TCA→TCG USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 111,516 C→A 26.1% Q196K (CAA→AAA)  USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 225,710 C→G 61.4% T266R (ACA→AGA)  USA300HOU_0205 → possible sugar phosphate isomerase
RA 299,913 T→A 8.7% H60Q (CAT→CAA USA300HOU_0266 → 2‑C‑methyl‑D‑erythritol 4‑phosphate cytidylyltransferase
RA 367,644 G→T 100% intergenic (‑96/‑20) nanA ← / → USA300HOU_0336 N‑acetylneuraminate lyase/possible transcriptional regulator
RA 479,553 G→A 100% A194T (GCA→ACA)  USA300HOU_0458 → hypothetical protein
RA 512,643 A→G 44.7% intergenic (+711/‑150) recR → / → rrs1 recombination protein RecR/16S ribosomal RNA
RA 531,823 A→G 13.4% intergenic (+1394/‑4331) USA300HOU_0494 → / → rplY hypothetical protein/ribosomal protein L25
RA 638,614 G→T 100% T14K (ACA→AAA)  thiD ← phosphomethylpyrimidine kinase
RA 707,879 A→T 100% L11I (TTA→ATA)  USA300HOU_0653 ← ABC superfamily ATP binding cassette transporter, ABC protein
RA 730,534 C→T 18.7% intergenic (+141/‑188) USA300HOU_0676 → / → USA300HOU_0677 possible S9C family peptidase/hypothetical protein
RA 787,110 C→T 16.6% R146C (CGC→TGC)  pabB → anthranilate synthase
RA 790,778 G→A 100% intergenic (+277/‑197) USA300HOU_0741 → / → USA300HOU_0742 possible allophanate hydrolase subunit 2/possible phosphatidylglycerol‑‑membrane‑oligosaccharide glycerophosphotransferase
RA 849,263 C→A 41.9% A130E (GCA→GAA)  USA300HOU_0794 → ATP‑binding protein
RA 880,093 G→T 33.3% intergenic (+271/‑70) USA300HOU_0822 → / → nuc hypothetical protein/micrococcal nuclease
RA 1,064,082 G→T 19.0% A136E (GCA→GAA)  USA300HOU_1000 ← LytR family transcriptional regulator
RA 1,226,031 G→T 15.2% V40L (GTA→TTA)  USA300HOU_1151 → hypothetical lipoprotein
RA 1,232,465 G→C 100% A124P (GCG→CCG)  USA300HOU_1157 → non‑specific serine/threonine protein kinase
RA 1,233,332 C→A 57.9% P413T (CCT→ACT)  USA300HOU_1157 → non‑specific serine/threonine protein kinase
RA 1,416,458 A→T 100% I216N (ATT→AAT)  USA300HOU_1314 ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
RA 1,440,007 Δ1 bp 100% coding (34/201 nt) cspA ← cold shock protein CspA
RA 1,474,639 C→G 100% A9129P (GCA→CCA)  ebh ← extracellular matrix binding protein
RA 1,481,355 G→T 100% A6890E (GCA→GAA)  ebh ← extracellular matrix binding protein
RA 1,513,930 A→T 100% L413F (TTA→TTT USA300HOU_1379 → hypothetical membrane protein
RA 1,646,189 G→C 100% A155G (GCA→GGA)  USA300HOU_1531 ← M24B family Xaa‑Pro dipeptidase
RA 1,650,833 A→C 11.6% S5A (TCA→GCA)  gcvPB ← glycine dehydrogenase (decarboxylating) subunit 2
RA 1,744,454 C→T 100% G73D (GGC→GAC)  apt ← adenine phosphoribosyltransferase
RA 1,775,439 C→T 62.6% G362D (GGT→GAT)  clpX ← S14 family endopeptidase ClpX
RA 1,797,772 G→C 100% R7G (CGC→GGC)  USA300HOU_1679 ← sensor histidine kinase
RA 2,010,363 C→T 100% C146Y (TGT→TAT)  USA300HOU_1861 ← ABC superfamily ATP binding cassette transporter, membrane protein
RA 2,013,179 A→T 30.2% Q19H (CAA→CAT USA300HOU_1864 → possible metal‑dependent hydrolase
RA 2,028,542 G→C 32.7% L35V (CTC→GTC)  USA300HOU_1882 ← hypothetical membrane protein
RA 2,279,705 G→A 100% intergenic (+1373/+6659) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
RA 2,367,543 T→C 10.5% intergenic (‑660/+4898) rpmC ← / ← USA300HOU_2234 ribosomal protein L29/NCS2 family nucleobase:cation symporter‑2
RA 2,532,298 A→T 100% T15T (ACA→ACT USA300HOU_2392 → FemAB family peptidoglycan biosynthesis protein
RA 2,856,087 T→A 100% P80P (CCT→CCA USA300HOU_2698 → hypothetical protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 512620 =40 (1.330)23 (1.100)
+40 bp
20/180 NT 45.5% intergenic (+688/‑173) recR/rrs1 recombination protein RecR/16S ribosomal RNA
?CP000730 1998278 = NA (NA)intergenic (‑6/+5) USA300HOU_t0050/rrf4 tRNA‑Val/5S ribosomal RNA
* ? CP000730 = 80092829 (0.960)3 (0.110) 3/246 NT 10.1% intergenic (+62/‑289) hisC1/USA300HOU_0748 histidinol‑phosphate transaminase/hypothetical protein
?CP000730 801948 = 26 (0.910)intergenic (+189/+435) USA300HOU_0748/dgkA1 hypothetical protein/possible diacylglycerol kinase