Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A19 F166 I0 R1 50 63.8 1389156 94.9% 1318309 140.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
JC CP000730 1,747,382 (TAC)3→2 96.3% coding (2065‑2067/2280 nt) secDF ← RND superfamily resistance‑nodulation‑cell division protein:proton (H+) antiporter

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 1747377 =1 (0.020)25 (0.450) 21/244 NT 96.3% coding (2072/2280 nt) secDF RND superfamily resistance‑nodulation‑cell division protein:proton (H+) antiporter
?CP000730 = 1747373 1 (0.020)coding (2076/2280 nt) secDF RND superfamily resistance‑nodulation‑cell division protein:proton (H+) antiporter

CAATCGTAACGTTTGACCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP000730/1747524‑1747377
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gtagtagtTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGAATAATGAAAAAACGTCAGTTGAAA  <  CP000730/1747373‑1747238
                                                                                                                                                                                                                                                                                            
aAATCGTAACGTTTGACCGTGAACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGCTCAGACAAGACAGTATTAACAGTTATTG                                                                                                                                                 <  2:321060/140‑1
 AATCGTAACGTTTGACCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGT                                                                                                                                                <  1:127662/141‑1
   TCGTAACGTTTGACCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAG                                                                                                                                              >  2:446500/1‑141
      gAACGTTTGACCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAG                                                                                                                                           <  1:326523/140‑1
          GTTTGACCGTGTAAGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGC                                                                                                                                       <  1:600114/141‑1
            TTGACCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTA                                                                                                                                     <  1:626020/141‑1
                        GTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCG                                                                                                                         <  1:485656/141‑1
                        GTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCG                                                                                                                         >  2:521739/1‑141
                          GAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGT                                                                                                                       <  2:690046/141‑1
                          GAAAACTAACAAAAGTTTAAAGTGATTACAACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGT                                                                                                                       <  2:279092/141‑1
                                  ACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTAC                                                                                                               >  2:81061/1‑141
                                   CAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTGTTCGGTGCTCCTACG                                                                                                              >  1:183161/1‑141
                                        GGTTAAAGTGATTACGACAACAGAACAAAGTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAACTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATT                                                                                                         <  2:74348/141‑1
                                                     ACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACTATATTCAACTTTACTTTg                                                                                            >  2:243787/1‑140
                                                     ACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTA                                                                                            >  1:36264/1‑141
                                                                   AATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGG                                                                              <  2:205768/141‑1
                                                                      TGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCCACTTTACCTTAGCATCATTTATCGGGTT                                                                           >  2:612823/1‑141
                                                                          GATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGAATTGATa                                                                       >  1:148472/1‑140
                                                                                        ATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTC                                                         >  2:551712/1‑141
                                                                                        ATCAAATAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTC                                                         <  2:334448/141‑1
                                                                                         TCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCACCATTTATCGGATAGATGTCTGGTGTAATCTCT                                                        >  1:94517/1‑141
                                                                                              TAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAAT                                                   <  1:111405/141‑1
                                                                                                 ACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGAGTTCTGGTGGATTCTCTTCAATCTT                                                >  1:279569/1‑141
                                                                                                 ACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATAGATTTCTGGTGTATTCTCTTCAATCTT                                                >  1:670412/1‑141
                                                                                                              CGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCG                                   >  2:3912/1‑141
                                                                                                               ggTCAATTATAACAGTATCTACGTTTATTGTAGTAGTTGCTATACGATTCTTCGGTGCTCCTACGATATTCAACTTTACTGTGTCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTTCCGTTCCtc                                  <  2:182829/139‑3
                                                                                                                 TCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTA                                >  2:200453/1‑141
                                                                                                                  CAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATGCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTAT                               <  2:516587/141‑1
                                                                                                                   AATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCatag                              >  1:596813/1‑137
                                                                                                                       AATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGA                          <  2:172928/141‑1
                                                                                                                                TTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGAAAAATGAAA                 >  1:113176/1‑141
                                                                                                                                         ATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGATGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGTGGAATAATAAAAAAACataaa        >  1:201329/1‑136
                                                                                                                                           TGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGGTCCGCTAGGGGGAATAATGAAAAAAgataagtt      >  1:277501/1‑133
                                                                                                                                              AGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGAATAATGAAAAAACGTCAGTTGAA   >  1:285524/1‑141
                                                                                                                                               GTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGAATAATGAAAAAACGTCAGTTGAAA  <  1:446500/141‑1
                                                                                                                                                                                                                                                                                            
CAATCGTAACGTTTGACCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP000730/1747524‑1747377
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gtagtagtTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGAATAATGAAAAAACGTCAGTTGAAA  <  CP000730/1747373‑1747238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.

GATK/CNVnator alignment

N/A