Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F184 I0 R1 46 69.8 1527574 94.8% 1448140 140.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
JC CP000730 1,747,382 (TAC)3→2 95.3% coding (2065‑2067/2280 nt) secDF ← RND superfamily resistance‑nodulation‑cell division protein:proton (H+) antiporter

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 1747377 =2 (0.030)39 (0.650) 31/242 NT 95.3% coding (2072/2280 nt) secDF RND superfamily resistance‑nodulation‑cell division protein:proton (H+) antiporter
?CP000730 = 1747373 2 (0.030)coding (2076/2280 nt) secDF RND superfamily resistance‑nodulation‑cell division protein:proton (H+) antiporter

AACGTTTGACCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP000730/1747517‑1747377
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gtagtagtTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGAATAATGAAAAAACGTCAGTTGAAAAAA  <  CP000730/1747373‑1747235
                                                                                                                                                                                                                                                                                        
AACGTTTGACCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTACCAGTTCTTTTAGTAG                                                                                                                                              >  2:139350/1‑140
  CGTTTGACCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAGTACAGAATTAAAAGTTCTTGTAGTAGTT                                                                                                                                            >  2:748100/1‑140
  CGTTTGACCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTATAGTAttg                                                                                                                                            >  2:634932/1‑137
   GTTTGACCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATCTCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTG                                                                                                                                           <  2:5260/140‑1
       tACCTTGTACGTGAAAACTTAGAAAAGGTTAAATTTATTACGACAACAGAACAAATTGATGATATGGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTAT                                                                                                                                       <  1:530638/139‑1
        ACCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTACTAACAGTTATTGTAGTACTTGCTATA                                                                                                                                      >  2:703350/1‑140
         CCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAAAAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATAC                                                                                                                                     <  1:576729/140‑1
           ggGTACGTGAAAACCTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAAACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTA                                                                                                                                   <  2:677381/138‑1
               ACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACAGTCAATTAATACAGTTTTAACAATTATTGTCGTAGATGCGATACTATTCT                                                                                                                               >  2:433124/1‑140
                   GAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACGAATTGGTGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGG                                                                                                                           >  2:499090/1‑140
                   GAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCAATACTATTCTTCGG                                                                                                                           >  1:651009/1‑140
                   GAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGAAAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGGTATACTATTCTTAGG                                                                                                                           >  1:216233/1‑140
                    AAAACTTAAAAAAGGTTATAGTGATTAAGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGT                                                                                                                          <  1:655493/140‑1
                                  GTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACTCGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCAAAACTATTCTTCAATGCTCATACGATATT                                                                                                            >  1:305394/1‑140
                                  GTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATT                                                                                                            >  1:538673/1‑140
                                  GTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATT                                                                                                            >  2:320422/1‑140
                                  GTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATT                                                                                                            >  2:490300/1‑140
                                   TTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTC                                                                                                           >  1:303659/1‑140
                                       AGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCACCT                                                                                                       >  2:669251/1‑140
                                          GATTACGACAACAGAACAAATTGATGATATCGTTAATAAAGCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTGCGATATTCAACCTTA                                                                                                    >  2:192496/1‑140
                                            TTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACT                                                                                                  >  1:303791/1‑140
                                            TTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGATATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACT                                                                                                  >  2:209807/1‑140
                                                  CAACAGAACAAATTGATTATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACATTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCA                                                                                            <  1:478795/140‑1
                                                      aggACAAATTGATGAAATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTAT                                                                                        <  1:125105/137‑1
                                                               TGATGATATCGTTAATAGATCCATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGAT                                                                               <  2:6015/140‑1
                                                                GATGATATCGTTAATAGATCAACTAGACAGACAAATACACGTTCAATTAATACAGTAGGAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATT                                                                              >  2:536640/1‑140
                                                                 ATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTG                                                                             <  2:628176/140‑1
                                                                 ATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGATGCTCCTACGATATTCAACTTTAATTTAGCAATATTTATCGGATTa                                                                             >  1:584416/1‑139
                                                                  TGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACCGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGATCCTAAGATATTCAACTTTACTTTAGCATTATTTATCGGATTGA                                                                            >  1:223541/1‑140
                                                                   GATATCGTTAATAGATCAATTCGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGACATTCAACTTTACTTTAGCATTATTTATCGGATTGAT                                                                           <  1:526070/140‑1
                                                                   GATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATAGGATTGAT                                                                           >  1:724374/1‑140
                                                                              TAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTAT                                                                <  1:634932/140‑1
                                                                               AGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTTATTTCTGGTGTATT                                                               >  1:543499/1‑140
                                                                                          ACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATAGGATTGATTTCTGGTGTATTTTCTTAAATCT                                                    >  1:261633/1‑140
                                                                                          ACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGAATTATTTATCAAATTGATTTCTGGTGTATTCTCTTCAATtt                                                    >  1:50717/1‑138
                                                                                              ACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCAT                                                >  2:646007/1‑140
                                                                                                      ACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTC                                        <  1:349604/140‑1
                                                                                                                AATACAGTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCGACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGG                              <  1:351381/140‑1
                                                                                                                  TACAGTATTAAGAGTTATTGTAGTAGTTGTTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGAA                            <  2:255971/140‑1
                                                                                                                      GTATTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCAGATTGATTTCTGGTGAATTCTCTTCAATCTTAATTGACGTTGCGCTAGGGGAAAAAAT                        >  1:6183/1‑140
                                                                                                                         TTAACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACCTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGAATAATGAA                     <  1:192496/140‑1
                                                                                                                            ACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGAATAATGGAAAA                  >  2:436278/1‑140
                                                                                                                            ACAGTTATTGTAGTAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTGTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCCTTGCCGTTCCGCTATGGGGAATACTGAAAca                  >  2:331622/1‑138
                                                                                                                                         TAGTTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACGTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGAATAATGAAAAAACGTCAGTTGAAA     <  2:86082/140‑1
                                                                                                                                         TAGTTGCTATACTATGCTTCGGTGCTCCTACGATATTCAAGTTCACTTTAGCATTATTTATCGGATTGAGTTCTGCTGTATTCTCTTCAATCCTCTTTGCCGTTCCGCTATGGGGAATAATGAAAAAACGTCAGTTGAAA     <  2:271357/140‑1
                                                                                                                                            TTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGAATAATGAAAAAACGTCAGTTGAAAAAA  <  1:87887/140‑1
                                                                                                                                                                                                                                                                                        
AACGTTTGACCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACGACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCAATTAATACAGTATTAACAGTTATTGTAGTAGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP000730/1747517‑1747377
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gtagtagtTGCTATACTATTCTTCGGTGCTCCTACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCAATCTTCATTGCCGTTCCGCTATGGGGAATAATGAAAAAACGTCAGTTGAAAAAA  <  CP000730/1747373‑1747235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.

GATK/CNVnator alignment

N/A