Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F22 I0 R2 95 29.9 669916 92.3% 618332 139.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 110,904 G→T 7.7% D255Y (GAT→TAT)  USA300HOU_0109 → staphylococcal tandem lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730110,9040GT 7.7% 209.6 / 4.0 65D255Y (GAT→TAT) USA300HOU_0109staphylococcal tandem lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (31/28);  new base T (2/3);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

CTCCAAAGTTATTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATG  >  CP000730/110767‑111044
                                                                                                                                         |                                                                                                                                            
cTCCAAAGTTATTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATg                                                                                                                                           <  1:309128/141‑1 (MQ=255)
cTCCAAAGTTATTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATg                                                                                                                                           <  1:17592/141‑1 (MQ=255)
    aaaGTTATTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGtata                                                                                                                                       >  2:37167/1‑141 (MQ=255)
    aaaGTTATTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTAAAAAAAAAAAAAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGtata                                                                                                                                       <  2:105609/141‑1 (MQ=25)
        ttATTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCa                                                                                                                                   <  1:205749/141‑1 (MQ=255)
          aTTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATg                                                                                                                                 <  1:28263/141‑1 (MQ=255)
          aTTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATg                                                                                                                                 >  2:309824/1‑141 (MQ=255)
                aaaaGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCaa                                                                                                                           <  2:231621/141‑1 (MQ=255)
                 aaaGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAAt                                                                                                                          >  1:333306/1‑141 (MQ=255)
                 aaaGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAAt                                                                                                                          >  1:333374/1‑141 (MQ=255)
                    ggTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAg                                                                                                                       <  1:126179/141‑1 (MQ=255)
                    ggTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTACAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAg                                                                                                                       <  1:203093/141‑1 (MQ=255)
                    ggTTCAGGGAATTTAAAAGGCTAATCAGTTGGATATAAAGATATTGAATTCAAGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAAGCAg                                                                                                                       <  2:285285/141‑1 (MQ=255)
                      ttCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGaa                                                                                                                     >  1:241916/1‑141 (MQ=255)
                         aGGGAATTTAAAAGGCTCATCAGCTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAatat                                                                                                                  >  2:133561/1‑141 (MQ=255)
                          gggAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATg                                                                                                                 >  1:311751/1‑141 (MQ=255)
                           ggAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGa                                                                                                                >  1:202592/1‑141 (MQ=255)
                                   aaaGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAaatcaatc                                                                                                        >  1:187785/1‑141 (MQ=255)
                                   aaaGGCTAATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAAAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAaatcaatc                                                                                                        <  2:251920/141‑1 (MQ=255)
                                    aaGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATcc                                                                                                       >  2:218111/1‑141 (MQ=255)
                                     aGGCTCATCAGTTGGATATAAAGATATTGAAGTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATccc                                                                                                      <  1:122750/141‑1 (MQ=255)
                                          catcaGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGaaa                                                                                                 <  1:212336/141‑1 (MQ=255)
                                           atcaGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGaaaa                                                                                                >  2:56828/1‑141 (MQ=255)
                                           atcaGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGaaaa                                                                                                >  2:226280/1‑141 (MQ=255)
                                            tcaGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGaaaaa                                                                                               <  1:298145/141‑1 (MQ=255)
                                            tcaGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGaaaaa                                                                                               >  1:41218/1‑141 (MQ=255)
                                                ttGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAagaga                                                                                           <  1:133083/141‑1 (MQ=255)
                                                    atatAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGtga                                                                                       >  1:241668/1‑141 (MQ=255)
                                                     tataAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGtgat                                                                                      >  1:231437/1‑141 (MQ=255)
                                                     tataAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGtgat                                                                                      <  1:314007/141‑1 (MQ=255)
                                                     aagaaaTATATTGAATTGAAGATTGTAGAGAAAAAAGAAGAAAATATATAATTTAGTGATAAATAAGAAAATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGtgat                                                                                      <  2:303246/138‑1 (MQ=255)
                                                        aaaGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGaa                                                                                   >  1:259704/1‑141 (MQ=255)
                                                         aaGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAGAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGaaa                                                                                  <  1:305968/141‑1 (MQ=255)
                                                         aaGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGaaa                                                                                  >  2:158734/1‑141 (MQ=255)
                                                           gATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATg                                                                                >  2:240523/1‑141 (MQ=255)
                                                            atatTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGa                                                                               >  1:109324/1‑141 (MQ=255)
                                                             tatTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGAt                                                                              <  1:78333/141‑1 (MQ=255)
                                                                 gAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATaaaa                                                                          >  1:181305/1‑141 (MQ=255)
                                                                      tACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATACAAGAAAAAGAGAGTGATGAAATGATAAAACgtgt                                                                     <  2:202592/141‑1 (MQ=255)
                                                                        cGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGtaa                                                                   >  1:226030/1‑141 (MQ=255)
                                                                        cGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGtaa                                                                   >  1:66281/1‑141 (MQ=255)
                                                                          ttagtaaaaaaaaaaaaAGAAAATATATACTTAAGTGATAGCTTAGATAATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGtaaat                                                                 <  1:7050/132‑1 (MQ=255)
                                                                              gagagAAAAAAGAAGAAAAGATATACTTTAGGGATAGCTTAGATTAGAAAAAAAGCGGAGATGTATGATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGAGGAAATGATAAAACGTGTaaataaat                                                             <  2:218052/140‑1 (MQ=255)
                                                                                 agaAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAAATTATAAAAAAAGAAAAACAGTCCAACCATAGATCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAg                                                          <  1:197680/141‑1 (MQ=255)
                                                                                  gaAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGt                                                         >  1:32702/1‑141 (MQ=255)
                                                                                   aagaaaGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGTGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTg                                                        >  2:282212/4‑141 (MQ=255)
                                                                                    aaaaaGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCAAAGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGc                                                       <  2:119642/141‑1 (MQ=255)
                                                                                      aaaGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCtt                                                     <  2:13618/141‑1 (MQ=255)
                                                                                          aagaaAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTa                                                 >  2:191895/1‑141 (MQ=255)
                                                                                              aaaTATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAg                                             >  1:15100/1‑141 (MQ=255)
                                                                                              aaaTATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAg                                             >  1:15087/1‑141 (MQ=255)
                                                                                                atatatACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGtc                                           <  1:37167/141‑1 (MQ=255)
                                                                                                  atataCTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGtctt                                         <  1:249530/141‑1 (MQ=255)
                                                                                                  atataCTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGtctt                                         <  1:256815/141‑1 (MQ=255)
                                                                                                    ataCTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGtcttct                                       <  2:162688/141‑1 (MQ=255)
                                                                                                         atcatGATAGCTCAAGTTATAAAAAAAGCGCCGATGTATAATCATAGCTCACTCTTTATATGAAAAAAAACAAAGAAAAAGAGAGTGATGAAATGATAAATCTTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGtttt                                  <  2:12357/137‑1 (MQ=255)
                                                                                                               aTAGCTTATAATAAAAAAAAAGCGGGGTTGTATAATCATGGCTCAATCAGAATATGAAATCCATCCCGGAAAAAGTGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCa                            <  2:331026/141‑1 (MQ=255)
                                                                                                                      aGATAATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAACAAATTAGTGCTTGGTATTAGTCTTCTGTTGTTAGTCATTAGTAt                     >  2:240659/1‑141 (MQ=255)
                                                                                                                          tataAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCAct                 >  1:145819/1‑141 (MQ=255)
                                                                                                                           ataAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCActg                >  1:56101/1‑141 (MQ=255)
                                                                                                                             aaaaaaaGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCActgct              >  2:278138/1‑141 (MQ=255)
                                                                                                                                 aaaGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGtt          <  1:5403/141‑1 (MQ=255)
                                                                                                                                   aGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTtgt        >  2:226888/1‑141 (MQ=255)
                                                                                                                                     aggAGATGTATAATCATGGCTCAATCAGAATATAAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTgg      <  1:222261/140‑1 (MQ=255)
                                                                                                                                      ggAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTAATAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGc     >  1:232665/1‑141 (MQ=255)
                                                                                                                                       gagaTGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCa    >  2:271732/1‑141 (MQ=255)
                                                                                                                                         gaTGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATg  >  2:96276/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                            
CTCCAAAGTTATTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATG  >  CP000730/110767‑111044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A