breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 35,814 Δ1 bp 21.7% intergenic (+45/+63) rlmH → / ← tpn hypothetical protein/IS431mec transposase
RA 35,817 A→T 22.2% intergenic (+48/+60) rlmH → / ← tpn hypothetical protein/IS431mec transposase
RA 35,850 C→T 23.3% intergenic (+81/+27) rlmH → / ← tpn hypothetical protein/IS431mec transposase
RA 35,858 T→A 27.7% intergenic (+89/+19) rlmH → / ← tpn hypothetical protein/IS431mec transposase
RA 35,876 C→T 34.1% intergenic (+107/+1) rlmH → / ← tpn hypothetical protein/IS431mec transposase
RA 35,913 C→G 23.4% S213S (TCG→TCC tpn ← IS431mec transposase
RA 35,940 G→A 27.1% R204R (CGC→CGT tpn ← IS431mec transposase
RA 35,970 T→C 58.6% E194E (GAA→GAG tpn ← IS431mec transposase
RA 35,997 T→C 30.2% T185T (ACA→ACG tpn ← IS431mec transposase
RA 36,015 C→T 26.1% R179R (AGG→AGA tpn ← IS431mec transposase
RA 36,087 A→G 60.1% C155C (TGT→TGC tpn ← IS431mec transposase
RA 36,119 C→T 24.0% V145I (GTA→ATA)  tpn ← IS431mec transposase
RA 36,124 G→A 58.9% A143V (GCT→GTT)  tpn ← IS431mec transposase
RA 36,133 A→G 24.8% V140A (GTA→GCA)  tpn ← IS431mec transposase
RA 36,161 T→C 28.4% I131V (ATT→GTT)  tpn ← IS431mec transposase
RA 36,174 T→C 26.5% K126K (AAA→AAG tpn ← IS431mec transposase
RA 36,269 C→T 63.7% A95T (GCA→ACA)  tpn ← IS431mec transposase
RA 36,315 G→A 58.9% Y79Y (TAC→TAT tpn ← IS431mec transposase
RA 36,378 C→T 82.3% L58L (TTG→TTA tpn ← IS431mec transposase
RA 36,413 C→T 48.8% V47I (GTC→ATC)  tpn ← IS431mec transposase
RA 36,444 C→T 39.3% R36R (AGG→AGA tpn ← IS431mec transposase
RA 36,471 A→G 14.4% S27S (AGT→AGC tpn ← IS431mec transposase
RA 76,180 C→T 16.7% intergenic (+238/‑896) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,185 G→A 14.2% intergenic (+243/‑891) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,265 C→T 24.2% intergenic (+323/‑811) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,295 T→C 50.6% intergenic (+353/‑781) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,322 G→A 48.6% intergenic (+380/‑754) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,353 G→A 43.8% intergenic (+411/‑723) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,969 G→A 79.2% intergenic (+1027/‑107) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 110,904 G→T 7.7% D255Y (GAT→TAT)  USA300HOU_0109 → staphylococcal tandem lipoprotein
RA 111,512 A→G 33.2% S194S (TCA→TCG USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 111,516 C→A 33.5% Q196K (CAA→AAA)  USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 111,560 T→C 20.7% R210R (CGT→CGC USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 134,761 A→T 12.9% K135M (AAG→ATG)  sbnA → possible pyridoxal‑phosphate dependent enzyme
RA 195,286 T→A 100% intergenic (‑97/‑82) USA300HOU_0183 ← / → USA300HOU_0184 hypothetical protein/hypothetical protein
RA 217,036 T→A 15.4% intergenic (‑203/+23) argD ← / ← brnQ ornithine‑‑oxo‑acid transaminase/possible LIVCS family branched chain amino acid:cation symporter
RA 253,973 C→T 11.8% intergenic (+186/‑2) USA300HOU_0226 → / → USA300HOU_0227 hypothetical protein/hypothetical protein
RA 396,540 G→A 86.7% A208T (GCA→ACA)  USA300HOU_0364 → possible lipoprotein
RA 512,643 A→G 57.4% intergenic (+711/‑150) recR → / → rrs1 recombination protein RecR/16S ribosomal RNA
RA 593,430 T→A 12.2% intergenic (+22/‑58) rpoC → / → USA300HOU_0538 DNA‑directed RNA polymerase subunit beta'/possible RNA‑binding ribosomal protein
RA 607,884 G→A 87.2% R209* (CGA→TGA)  dck ← deoxynucleoside kinase
RA 629,681 C→A 100% L84I (CTA→ATA)  hxlA → possible 3‑hexulose‑6‑phosphate synthase
RA 665,392 T→G 100% L79* (TTA→TGA)  iolS → possible acetytransferase
RA 693,258 T→A 11.1% I65F (ATT→TTT)  USA300HOU_0636 ← hypothetical protein
RA 707,910 A→G 100% intergenic (‑1/‑121) mntB ← / → sirR ABC superfamily ATP binding cassette transporter, ABC protein/possible metal‑dependent transcriptional regulator
RA 1,105,994 T→C 16.9% T12T (ACT→ACC pdhD → dihydrolipoyl dehydrogenase
RA 1,233,504 T→A 100% V470D (GTT→GAT)  prkC → non‑specific serine/threonine protein kinase
RA 1,416,458 A→T 100% I216N (ATT→AAT)  oppD ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
RA 1,440,085 G→T 100% intergenic (‑45/‑891) cspA ← / → xpaC cold shock protein CspA/5‑bromo‑4‑chloroindolyl phosphate hydrolysis protein XpaC
RA 1,513,930 A→T 100% L413F (TTA→TTT USA300HOU_1379 → hypothetical membrane protein
RA 1,539,250 C→G 22.6% intergenic (‑349/+37) aroC ← / ← USA300HOU_1405 chorismate synthase/hypothetical protein
RA 1,579,073 T→C 12.7% D85G (GAT→GGT)  USA300HOU_1445 ← major tail protein
RA 1,608,818 G→A 14.3% T20M (ACG→ATG)  USA300HOU_1492 ← possible lipoprotein
RA 1,646,189 G→C 100% A155G (GCA→GGA)  pepP ← M24B family Xaa‑Pro dipeptidase
RA 1,715,153 T→C 10.5% D33G (GAC→GGC)  accB ← acetyl‑CoA carboxylase biotin carboxyl carrier subunit
RA 1,744,362 G→A 100% H104Y (CAC→TAC)  apt ← adenine phosphoribosyltransferase
RA 1,761,889 C→A 100% D388Y (GAT→TAT)  folC ← tetrahydrofolate synthase
RA 1,929,796 T→C 22.7% intergenic (‑879/‑289) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,797 G→A 22.3% intergenic (‑880/‑288) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,799 C→A 22.3% intergenic (‑882/‑286) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,804 A→G 22.2% intergenic (‑887/‑281) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,812 G→A 35.6% intergenic (‑895/‑273) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,842 Δ1 bp 100% intergenic (‑925/‑243) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,843 Δ1 bp 51.0% intergenic (‑926/‑242) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,848 C→A 48.8% intergenic (‑931/‑237) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,861 T→C 48.0% intergenic (‑944/‑224) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,862:1 +T 37.0% intergenic (‑945/‑223) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,864 A→T 11.0% intergenic (‑947/‑221) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,865 A→C 37.1% intergenic (‑948/‑220) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,866 G→T 47.5% intergenic (‑949/‑219) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,867 G→T 47.1% intergenic (‑950/‑218) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,870 A→C 47.2% intergenic (‑953/‑215) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,872 G→A 46.1% intergenic (‑955/‑213) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,873 C→A 46.0% intergenic (‑956/‑212) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,875 T→C 47.5% intergenic (‑958/‑210) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,917 C→T 38.7% intergenic (‑1000/‑168) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,918 A→G 36.3% intergenic (‑1001/‑167) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,925 T→A 40.9% intergenic (‑1008/‑160) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,928 T→A 40.8% intergenic (‑1011/‑157) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,930 T→A 39.4% intergenic (‑1013/‑155) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,941 A→T 32.3% intergenic (‑1024/‑144) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,946 G→A 31.2% intergenic (‑1029/‑139) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,963,768 C→G 100% V365L (GTA→CTA)  hemG ← protoporphyrinogen oxidase
RA 2,010,363 C→T 100% C146Y (TGT→TAT)  ygaD ← ABC superfamily ATP binding cassette transporter, membrane protein
RA 2,279,705 G→A 100% intergenic (+1373/+6659) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
RA 2,337,959 C→T 13.2% intergenic (‑125/‑119) adhR ← / → USA300HOU_2196 possible MerR family transcriptional regulator/hypothetical protein
RA 2,352,815 G→A 85.1% A92V (GCA→GTA)  rpsI ← ribosomal protein S9
RA 2,372,419 C→T 15.1% intergenic (‑5536/+22) rpmC ← / ← pbuG ribosomal protein L29/NCS2 family nucleobase:cation symporter‑2
RA 2,394,308 G→A 9.5% A85V (GCA→GTA)  moaB ← molybdopterin cofactor biosynthesis protein MoaB
RA 2,454,626 A→T 91.8% L48L (CTA→CTT USA300HOU_2315 → hypothetical membrane protein
RA 2,532,298 A→T 100% T15T (ACA→ACT murN → FemAB family peptidoglycan biosynthesis protein
RA 2,670,935 G→A 14.8% A60V (GCG→GTG)  USA300HOU_2525 ← esterase
RA 2,732,937 C→G 9.3% R311R (CGC→CGG USA300HOU_2590 → possible S15 family peptidase
RA 2,845,036 T→G 8.6% K93Q (AAA→CAA)  USA300HOU_2686 ← ABC superfamily ATP‑binding cassette transporter, membrane protein
RA 2,855,054 G→T 11.8% T190T (ACC→ACA rarD ← DMT superfamily drug/metabolite transporter

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 512620 =26 (0.940)30 (1.580)
+40 bp
27/178 NT 62.7% intergenic (+688/‑173) recR/rrs1 recombination protein RecR/16S ribosomal RNA
?CP000730 1998278 = NA (NA)intergenic (‑6/+5) USA300HOU_t0050/rrf4 tRNA‑Val/5S ribosomal RNA
* ? CP000730 760026 =41 (1.490)3 (0.120) 3/230 NT 7.6% coding (1674/1674 nt) cydC ABC superfamily ATP binding cassette transporter, ABC protein
?CP000730 760070 = 36 (1.470)intergenic (+44/+83) cydC/USA300HOU_0709 ABC superfamily ATP binding cassette transporter, ABC protein/possible MarR family transcriptional regulator
* ? CP000730 = 80275719 (0.690)3 (0.120) 3/244 NT 15.0% coding (544/918 nt) dgkA1 possible diacylglycerol kinase
?CP000730 = 802799 16 (0.610)coding (502/918 nt) dgkA1 possible diacylglycerol kinase
* ? CP000730 = 104583229 (1.050)5 (0.190) 3/248 NT 15.2% intergenic (+9/+43) USA300HOU_0988/menA hypothetical protein/1,4‑dihydroxy‑2‑naphthoate octaprenyltransferase
?CP000730 = 1045855 28 (1.060)intergenic (+32/+20) USA300HOU_0988/menA hypothetical protein/1,4‑dihydroxy‑2‑naphthoate octaprenyltransferase
* ? CP000730 1131036 =29 (1.050)3 (0.130) 3/224 NT 12.2% coding (435/435 nt) ylbF hypothetical protein
?CP000730 1131101 = 18 (0.750)intergenic (+65/‑1163) ylbF/USA300HOU_1059 hypothetical protein/hypothetical protein
* ? CP000730 = 137364232 (1.160)4 (0.160) 4/236 NT 11.7% intergenic (+663/‑1232) yneF/sbcC hypothetical protein/exodeoxyribonuclease SbcC
?CP000730 = 1373669 31 (1.230)intergenic (+690/‑1205) yneF/sbcC hypothetical protein/exodeoxyribonuclease SbcC
* ? CP000730 = 206655830 (1.090)3 (0.110) 3/248 NT 9.3% coding (214/561 nt) pncA nicotinamidase
?CP000730 = 2066576 30 (1.130)coding (232/561 nt) pncA nicotinamidase
* ? CP000730 = 244005226 (0.940)3 (0.120) 3/234 NT 11.4% intergenic (+13/+53) USA300HOU_2302/glvC hypothetical protein/PTS family arbutin‑salicin‑cellobiose (ASC) porter component IIBC
?CP000730 = 2440086 23 (0.920)intergenic (+47/+19) USA300HOU_2302/glvC hypothetical protein/PTS family arbutin‑salicin‑cellobiose (ASC) porter component IIBC
* ? CP000730 = 274638425 (0.910)5 (0.190) 5/242 NT 17.4% intergenic (+7/+53) USA300HOU_2601/acsA2 hypothetical protein/acetate‑‑CoA ligase
?CP000730 = 2746433 24 (0.930)intergenic (+56/+4) USA300HOU_2601/acsA2 hypothetical protein/acetate‑‑CoA ligase