Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F121 I0 R1 103 70.6 1582072 92.0% 1455506 141.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 587,957 G→T 58.6% A621S (GCG→TCG)  rpoB → DNA‑directed RNA polymerase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730587,9570GT58.6% 37.6 / 66.5 67A621S (GCG→TCG) rpoBDNA‑directed RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (13/15);  new base T (29/10);  total (42/25)
Fisher's exact test for biased strand distribution p-value = 2.39e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

ACAAGCAAACTCTAAATTAGATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGAACACGT  >  CP000730/587818‑588087
                                                                                                                                           |                                                                                                                                   
acaAGCAAACACTAAATTAGATGAAAATTGTCGTTTAATGGATGATGAAGTTGTACGTCGTTTCCGTGGTAACAACACAGTTAAGGCTAAAGTAAAAAAGGATTATAGGGATGTATGGCTGAAGCATGTTGTGTCagcagc                                                                                                                                    <  2:349850/141‑1 (MQ=255)
    caataCTCTAAATTAGATGGAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACa                                                                                                                                <  2:354299/137‑1 (MQ=255)
      aaaatcaaaatcAGATGAAAATCGTAGATTAAAAGATAATGAAGTAATATGTAGATCAAGTGGAAACAATAAAGTTAAAAAAAAAAAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGc                                                                                                                              <  2:712496/141‑1 (MQ=255)
      aaaCTCTAAATTAGATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAGCAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGATGTTTCAGCAGCGACAGc                                                                                                                              >  2:131750/1‑141 (MQ=255)
      aaaCTCTAAATTAGATGAAAATGGTCGTTTCATGGATGATGAAGTTATATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGAATCGACAGc                                                                                                                              >  2:503416/1‑141 (MQ=255)
       atagctAAATTAGATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCa                                                                                                                             <  1:190550/137‑1 (MQ=255)
       aaCTCTAAATTAGATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCa                                                                                                                             >  2:485922/1‑141 (MQ=255)
        aCTCTAAATTAGATGAAAATGGACGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGAAAGCAt                                                                                                                            >  2:739920/1‑141 (MQ=255)
         ctctAAATTAGATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATg                                                                                                                           <  1:201907/141‑1 (MQ=255)
           ctAAATTAGATGAAACTGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTCACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGGTGTTTCAGCATCGACAGCATGTa                                                                                                                         >  1:391183/1‑141 (MQ=255)
             aaaTTAGATGAAAATGGTCGTTTCATGGAGGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTAtt                                                                                                                       <  2:595269/141‑1 (MQ=255)
                       aaaaTGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGCCAGCATGTATTC‑CAGTCTTAg                                                                                                            >  2:615768/1‑141 (MQ=255)
                       aaaaTGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCAACAGCATGTATTA‑CATTCTTAg                                                                                                            >  2:17051/1‑141 (MQ=255)
                        aaaTGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTC‑CATTCTTAGa                                                                                                           >  1:760400/1‑141 (MQ=255)
                            ggTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGTAAGTTGTTTCAGCATCGACAGCATGTATTC‑CATTCTTAGAAAat                                                                                                       >  2:405434/1‑141 (MQ=255)
                            ggTCGTTTCATGGATGATGAAGTTGTATGTCGTGTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTC‑CATTCTTAGAAAat                                                                                                       >  2:612712/1‑141 (MQ=255)
                             gTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATCCAGTTATGGCTAACGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTC‑CATTATTAGAAAatg                                                                                                      >  1:86523/1‑141 (MQ=255)
                              tCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTC‑CATTCTTAGAAAatga                                                                                                     >  1:401601/1‑141 (MQ=255)
                                gTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTC‑CATTCTTAGAAAatgatg                                                                                                   <  1:387853/141‑1 (MQ=255)
                                  ttCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTC‑CATTCTTAGAAAATGATGAc                                                                                                 >  1:107398/1‑141 (MQ=255)
                                      tGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGAATATAGGGATGTATCGCCGAAGCAAGTTGTTTCAGAATCGACAGCATGTATTC‑CAATCTTAGACAAagatgaacgaa                                                                                             >  2:584395/1‑136 (MQ=255)
                                       ggATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCaaa                                                                                            <  1:730479/141‑1 (MQ=255)
                                          tgatgaAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCg                                                                                         >  2:127331/1‑141 (MQ=255)
                                              gaAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTC‑CATTCTTAGAAAAGCATGACTCAAACCGAGCa                                                                                     >  2:528378/1‑141 (MQ=255)
                                               aaGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCATGCAt                                                                                    >  2:525658/1‑141 (MQ=255)
                                                 gTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTg                                                                                  >  2:734846/1‑141 (MQ=255)
                                                 gTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTg                                                                                  <  1:210895/141‑1 (MQ=255)
                                                     tATGTCGTTTCCGTGGCAACAATACAGTTATGGCTAAACAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGATgg                                                                              <  1:9049/141‑1 (MQ=255)
                                                      aTGTCGTGTCCGTGGTAAAAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGATggg                                                                             <  2:33574/141‑1 (MQ=255)
                                                          cGTTTCCGTTGTAAAAATACAGTTATGGCTAAAGAAAAAATGTATATCATATATGTATCTCCTTAGCAAGATGTATTAGCAGAGAGAGCATTTATTC‑CATGCTTAGAATATGATGACTCAAACCGTGTATTGATGGGTGCg                                                                         <  2:590086/141‑1 (MQ=255)
                                                          cGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCg                                                                         <  1:43555/141‑1 (MQ=255)
                                                              tCCGTGGTAACAATGCAGTTATGGCTAAAGAAAAAATGGATTATATGTATGTATCGCCGAACCAAGTTGTTTCAGCAGCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACa                                                                     <  2:38179/141‑1 (MQ=255)
                                                               ccGTGGTAACAATACAGTTAGGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTC‑CATTCTTAGAAAATGCTGTCTCAAACCGTGCATTGATGGGTGCGAACAt                                                                    <  2:134057/141‑1 (MQ=255)
                                                                cGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATg                                                                   >  2:572308/1‑141 (MQ=255)
                                                                 gTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCCACCCGTGCATTGATGGGTGCGAACATga                                                                  <  2:661275/141‑2 (MQ=255)
                                                                    gTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCCACAGCCTGTATTA‑CATTTGTTGAAAATGATGACTCAAGCCGTGCATTGATGGGTGCGAACATGCAAc                                                               <  2:166997/141‑1 (MQ=255)
                                                                     tAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGGGAACATGAAACg                                                              >  2:367930/1‑141 (MQ=255)
                                                                     tAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACg                                                              <  1:63814/141‑1 (MQ=255)
                                                                     tAACAATACAGTGATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCGGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGCTGGGTGCGAACATTCAAcc                                                              >  2:618292/1‑140 (MQ=255)
                                                                           tACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGc                                                        >  1:606356/1‑141 (MQ=255)
                                                                             cAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAg                                                      <  1:670926/141‑1 (MQ=255)
                                                                                   tGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCCACGGCCAGCAGTACCtt                                                >  1:232358/1‑141 (MQ=255)
                                                                                    ggCTAAAGAAAAAATGAATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGGGCGAACATGCAACGCCAAGCCGTGCCttt                                               >  1:469371/1‑141 (MQ=255)
                                                                                      cTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGGACATGCCACGCCAAGCAGTGCCTTtga                                             >  2:703364/1‑141 (MQ=255)
                                                                                           gAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAAt                                        >  1:423968/1‑141 (MQ=255)
                                                                                            aaaaaaTGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATGC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATc                                       >  1:777117/1‑141 (MQ=255)
                                                                                              aaaaTGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCa                                     >  2:773037/1‑141 (MQ=255)
                                                                                              aaaaTGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGCGCCTTTTATGAATCCa                                     >  2:165054/1‑141 (MQ=255)
                                                                                                   ggATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGc                                >  1:438230/1‑141 (MQ=255)
                                                                                                   ggATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCCTTAATGAATCCAGAAGc                                >  2:258208/1‑141 (MQ=255)
                                                                                                   ggATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGc                                >  1:687456/1‑141 (MQ=255)
                                                                                                          atGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGAAGTGCCTGTGATGAATCCAGAAGCACCAttt                         <  2:760400/141‑1 (MQ=255)
                                                                                                           tggatggaTCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGAGCGAACATGCAACGTCAAACAGTGCCGTTGATGAATCCAGAAGAACCATttg                        >  2:496014/1‑141 (MQ=255)
                                                                                                            ggATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCCACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATgtgt                       >  2:472681/1‑141 (MQ=255)
                                                                                                            ggATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTC‑CATTCTAAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATttgt                       >  2:482802/1‑141 (MQ=255)
                                                                                                             gATGTATCGCGGAAGCCAGTTGTTTCAGCAGCGACAGCATGTATTCTTTTTCTTTTGAAA‑GATGAATAAAACCGTTCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATttgtt                      <  1:149357/141‑1 (MQ=255)
                                                                                                                  aTCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTAc                 >  1:484113/1‑141 (MQ=255)
                                                                                                                   tCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACa                <  1:43585/141‑1 (MQ=255)
                                                                                                                         aaGCAAGTTGTTTCAGCATCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGCACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAAAAGCACCACTTGTTGGCTCCGGTAtc          >  2:614232/1‑140 (MQ=255)
                                                                                                                          aggaaGTTGTTTCAGCAGCGACAGCATGTATTC‑CATTCTTAGAAAAGGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATgg         <  2:184512/138‑1 (MQ=255)
                                                                                                                            cAAGTTGTTTCAGCATCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGaa       >  1:69371/1‑141 (MQ=255)
                                                                                                                            cAAGTTGTTTCAGCATCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGaa       <  1:91974/141‑1 (MQ=255)
                                                                                                                            cAAGTTGTTTCAGCAGCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGaa       <  1:384851/141‑1 (MQ=255)
                                                                                                                            cAAGTTGTTTCAGCAGCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACCCAAACCGTGAATTGATGGTTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGaa       <  1:775648/141‑1 (MQ=255)
                                                                                                                               gttgttTCAGCATCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGCacac    >  1:591785/1‑141 (MQ=255)
                                                                                                                               gttgttTCAGCATCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGGATGGAaccc    >  2:110065/1‑139 (MQ=255)
                                                                                                                               gttgttTCAGCATCGACAGCATGTATTC‑CATTCTTAGAAAATCATGCGCCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTAGAGGTATGGAacac    >  1:615382/1‑141 (MQ=255)
                                                                                                                                 tgttTCAGCAGCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTAAAGGTATGGAACACGt  >  2:414741/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                   
ACAAGCAAACTCTAAATTAGATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTC‑CATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGAACACGT  >  CP000730/587818‑588087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A