Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F121 I1 R1 10 91.9 2094364 90.6% 1897493 139.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 587,957 G→T A621S (GCG→TCG)  rpoB → DNA‑directed RNA polymerase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730587,9570GT84.7% 225.5 / 46.2 98A621S (GCG→TCG) rpoBDNA‑directed RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (5/10);  new base T (30/53);  total (35/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

ACAAGCAAACTCTAAATTAGATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGAACACG  >  CP000730/587818‑588086
                                                                                                                                           |                                                                                                                                 
acaAGCAAACTCTAAATTAGATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCagcagc                                                                                                                                  >  2:660705/1‑141 (MQ=255)
       aaCTCTAAAATAGAAAAAAAAACTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTAAAAAAAAAAAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCa                                                                                                                           <  2:360257/141‑1 (MQ=255)
           ctAAATTAGATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTa                                                                                                                       >  1:787472/1‑141 (MQ=255)
           ctAAATTAGATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTa                                                                                                                       >  2:809952/1‑141 (MQ=255)
             aaaTTAGATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGACGTATCGCCGAAGCAAGTTGTCTCAGCAGCGACAGCATGTact                                                                                                                     >  2:186404/1‑139 (MQ=255)
               aTTAGATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTcc                                                                                                                   >  2:138040/1‑141 (MQ=255)
                 tAGATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCAt                                                                                                                 <  1:660705/141‑1 (MQ=255)
                   gATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTACAGAAAAAATGGGAGATATGGAGGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTc                                                                                                               >  1:470805/1‑141 (MQ=255)
                   gATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTc                                                                                                               >  2:836538/1‑141 (MQ=255)
                     tGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCtt                                                                                                             <  1:137111/141‑1 (MQ=255)
                     tGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCtt                                                                                                             <  1:840006/141‑1 (MQ=255)
                      gAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTa                                                                                                            <  2:484127/141‑1 (MQ=255)
                      gAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTa                                                                                                            <  2:81622/141‑1 (MQ=255)
                       aaaaTGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAg                                                                                                           >  1:978601/1‑141 (MQ=255)
                       aaaaTGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAg                                                                                                           >  2:875552/1‑141 (MQ=255)
                        aaaTGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGa                                                                                                          <  1:1025732/141‑1 (MQ=255)
                        aaaTGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGa                                                                                                          <  1:341182/141‑1 (MQ=255)
                           tGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTAAGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGaaaa                                                                                                       <  2:744791/141‑1 (MQ=255)
                              tCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAatga                                                                                                    <  2:489368/141‑1 (MQ=255)
                               cGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAatgat                                                                                                   >  1:882103/1‑141 (MQ=255)
                                gTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTTGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAatgatg                                                                                                  <  2:900473/141‑1 (MQ=255)
                                gTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAatgatg                                                                                                  <  1:480594/141‑1 (MQ=255)
                                 tttCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAAtgatga                                                                                                 >  1:110801/1‑141 (MQ=255)
                                 tttCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAAtgatga                                                                                                 >  2:577332/1‑141 (MQ=255)
                                  ttCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTGAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGAc                                                                                                >  1:283370/1‑141 (MQ=255)
                                     aTGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCa                                                                                             >  2:484792/1‑141 (MQ=255)
                                       ggATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCaaa                                                                                           >  2:561223/1‑141 (MQ=255)
                                        gatgatGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGATAAAAAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAAc                                                                                          <  2:1016857/141‑1 (MQ=255)
                                         atgatgAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTA‑ATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCg                                                                                        <  2:257114/141‑1 (MQ=255)
                                          tgatgaAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCg                                                                                        <  1:964741/141‑1 (MQ=255)
                                           gatgaAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGt                                                                                       <  1:367226/141‑1 (MQ=255)
                                              gaAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCa                                                                                    <  1:867956/141‑1 (MQ=255)
                                              gaAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCa                                                                                    <  2:761475/141‑1 (MQ=255)
                                              gaAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCa                                                                                    <  1:402793/141‑1 (MQ=255)
                                               aaGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCAt                                                                                   <  2:911523/141‑1 (MQ=255)
                                               aaGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCCAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCAt                                                                                   >  2:899496/1‑141 (MQ=255)
                                                aGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCAtt                                                                                  <  1:689921/141‑1 (MQ=255)
                                                aGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCAtt                                                                                  <  2:527937/141‑1 (MQ=255)
                                                 gTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTg                                                                                 <  1:601915/141‑1 (MQ=255)
                                                    gTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATg                                                                              <  2:106774/141‑1 (MQ=255)
                                                    gTATGTCGTTTCCGTGGTAACAATACAGTTAAAAATAAAAAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATg                                                                              <  2:106450/141‑1 (MQ=255)
                                                      aTGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATggg                                                                            <  2:258332/141‑1 (MQ=255)
                                                      aTGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATggg                                                                            >  1:105275/1‑141 (MQ=255)
                                                       tGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGt                                                                           >  1:293689/1‑141 (MQ=255)
                                                          cGTTTCCGTGGTAACAATACAGTTATGGCTAAAGCAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCg                                                                        <  1:968914/141‑1 (MQ=255)
                                                          cGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCg                                                                        <  2:391197/141‑1 (MQ=255)
                                                             ttCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAAc                                                                     >  2:762030/1‑141 (MQ=255)
                                                              tCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACa                                                                    <  2:520387/141‑1 (MQ=255)
                                                               ccGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACAt                                                                   <  2:295547/141‑1 (MQ=255)
                                                               ccGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACAt                                                                   <  2:295548/141‑1 (MQ=255)
                                                                cGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATg                                                                  >  1:370572/1‑141 (MQ=255)
                                                                 gTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGc                                                                 <  1:671412/141‑1 (MQ=255)
                                                                  tGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCa                                                                <  1:203014/141‑1 (MQ=255)
                                                                    gTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAAc                                                              >  2:794560/1‑141 (MQ=255)
                                                                     tAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACg                                                             <  1:704889/141‑1 (MQ=255)
                                                                      aaCAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGt                                                            <  1:585949/141‑1 (MQ=255)
                                                                          aTACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAg                                                        >  2:182815/1‑141 (MQ=255)
                                                                            acagaaaaaaaaaaaGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCa                                                      <  2:67665/129‑1 (MQ=255)
                                                                            aCAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCa                                                      >  2:842239/1‑141 (MQ=255)
                                                                             cAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAg                                                     <  1:668570/141‑1 (MQ=255)
                                                                             cAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAg                                                     <  2:451801/141‑1 (MQ=255)
                                                                             cAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAg                                                     <  2:890298/141‑1 (MQ=255)
                                                                             cAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAg                                                     <  1:343636/141‑1 (MQ=255)
                                                                             cAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAg                                                     <  1:759478/141‑1 (MQ=255)
                                                                              aGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGt                                                    <  2:874498/141‑1 (MQ=255)
                                                                               gTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTg                                                   >  2:386516/1‑141 (MQ=255)
                                                                               gTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTg                                                   >  2:506498/1‑141 (MQ=255)
                                                                                 tATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGcc                                                 <  1:638118/141‑1 (MQ=255)
                                                                                   tGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCtt                                               >  2:802494/1‑141 (MQ=255)
                                                                                       tAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTtgat                                           >  2:9740/1‑141 (MQ=255)
                                                                                        aaaGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTtgatg                                          <  1:850455/141‑1 (MQ=255)
                                                                                        aaaGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTtgatg                                          <  1:644561/141‑1 (MQ=255)
                                                                                        aaaGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTtgatg                                          <  1:135088/141‑1 (MQ=255)
                                                                                        aaaGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTtgatg                                          <  1:809851/141‑1 (MQ=255)
                                                                                        aaaGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTtgatg                                          <  1:300818/141‑1 (MQ=255)
                                                                                              aaaaTTTATGATATCGATGTATCGCCGAAGCAAGGTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAATACTATTGTTCAATGCTTTTATATATTTTTGCGAACATGCAACTTCAGGCAGTGCCTTTTATGAATCCa                                    <  2:833385/141‑1 (MQ=255)
                                                                                               aaaTGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAg                                   >  1:872075/1‑141 (MQ=255)
                                                                                                  tGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAg                                <  2:110801/141‑1 (MQ=255)
                                                                                                    gATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCa                              <  1:138040/141‑1 (MQ=255)
                                                                                                    gATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCa                              <  1:820782/141‑1 (MQ=255)
                                                                                                    gATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCa                              >  2:121914/1‑141 (MQ=255)
                                                                                                    gATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCa                              <  1:234235/141‑1 (MQ=255)
                                                                                                     aTTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCAATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCAc                             <  2:207364/141‑1 (MQ=255)
                                                                                                         tatGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCAtt                         <  1:279124/141‑1 (MQ=255)
                                                                                                          atGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCAttt                        <  1:291729/141‑1 (MQ=255)
                                                                                                          atGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCAttt                        >  1:136837/1‑141 (MQ=255)
                                                                                                               tGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGGAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTgg                   >  1:532244/1‑141 (MQ=255)
                                                                                                                    cGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAg              <  1:101897/141‑1 (MQ=255)
                                                                                                                     gCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAgg             >  1:372211/1‑141 (MQ=255)
                                                                                                                     gCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAgg             <  2:1519/141‑1 (MQ=255)
                                                                                                                      ccGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGt            >  1:630173/1‑141 (MQ=255)
                                                                                                                          aGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATgg        <  2:843471/141‑1 (MQ=255)
                                                                                                                          aGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATgg        <  1:228582/141‑1 (MQ=255)
                                                                                                                          aGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATgg        <  1:134934/141‑1 (MQ=255)
                                                                                                                          aGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATgg        <  2:419259/141‑1 (MQ=255)
                                                                                                                           gCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGa       >  1:250469/1‑141 (MQ=255)
                                                                                                                           gCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGa       >  2:281545/1‑141 (MQ=255)
                                                                                                                              aGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGAacc    >  2:608060/1‑140 (MQ=255)
                                                                                                                                ttgttTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGAACACg  <  1:263909/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                 
ACAAGCAAACTCTAAATTAGATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGAACACG  >  CP000730/587818‑588086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A