Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F67 I0 R1	98	84.8	1894234	92.4%	1750272	141.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	587,957	G→T	11.4%	A621S (GCG→TCG)	rpoB →	DNA‑directed RNA polymerase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	587,957	0	G	T	11.4%	241.2 / 14.3	86	A621S (GCG→TCG)	rpoB	DNA‑directed RNA polymerase subunit beta
Reads supporting (aligned to +/- strand): ref base G (31/44); new base T (8/2); total (39/47)
Fisher's exact test for biased strand distribution p-value = 3.87e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

CACAAGCAAACTCTAAATTAGATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGAACACGTTGCAGCACGT  >  CP000730/587817‑588097

cacaAGCAAACTCTAAATTAGATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATTGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTcagcag                                                                                                                                               <  2:730343/141‑1 (MQ=255)

       aaaCTCTAAATTAGAGGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCTGTGGTATCAATACAGTTAAAAAAAAAAAAAAAATGGATTATATGGATGTATCGCCAAAGCAAGTTGTTTCAGCAGCGACAGc                                                                                                                                        <  2:52311/141‑1 (MQ=255)

           tctAAATTAGATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGCTTATATGGATGTATCGCCGAACCAACTTGTTTCAGCATCGCCACCATGt                                                                                                                                    >  2:214278/1‑141 (MQ=255)

           tctAAATTAGATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGt                                                                                                                                    >  1:554896/1‑141 (MQ=255)

           tctAAATTAGATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGt                                                                                                                                    <  1:848636/141‑1 (MQ=255)

              aaaGTAGATGAAACTGGTCGTTTCATGGATGATGTAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCGAGTTGTTTCAGCAGCGACAGCATGTAtt                                                                                                                                 <  2:559153/141‑1 (MQ=255)

                    gATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAAAAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTc                                                                                                                           <  2:399664/141‑1 (MQ=255)

                     aTGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCt                                                                                                                          <  2:480377/141‑1 (MQ=255)

                         aaaTGGTCTTTTCATGGATGATGCATTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGTATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGa                                                                                                                      <  2:365289/141‑1 (MQ=255)

                         aaaTGGTCGTTTCACGGATGACTAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGa                                                                                                                      <  1:101532/141‑1 (MQ=255)

                            tGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGGGACAGCATGTATTCCTTTCTTAGaaaa                                                                                                                   >  1:99617/1‑141 (MQ=255)

                            tGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCCTTCTTAGaaaa                                                                                                                   >  1:483709/1‑141 (MQ=255)

                              gTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAatg                                                                                                                 >  1:805476/1‑141 (MQ=255)

                                cGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTCAAGAAAAAATGGATTATATGGAGGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTGTTCCATTTTTAGagaagcaa                                                                                                               >  1:843451/1‑137 (MQ=255)

                                cGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAatgat                                                                                                               >  2:46410/1‑141 (MQ=255)

                                cGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCCTGTATTCCATTCTTAGAAAatgat                                                                                                               >  2:254430/1‑141 (MQ=255)

                                cGTTTCATAGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCATCAGCGACAGCATGTATTCCATTCTTAGAAAatgat                                                                                                               <  1:814525/141‑1 (MQ=255)

                                      attgatgattgatgtGTATGTCGTTTCCTTAGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCTACAGCATGTATTCCATTCTTAGAAAATGATGACTCa                                                                                                         <  2:694464/127‑1 (MQ=255)

                                      aTGGATGATGCAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGATACAGGGCTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCa                                                                                                         >  1:681815/1‑141 (MQ=255)

                                        ggATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCaaa                                                                                                       <  2:933626/141‑1 (MQ=255)

                                        ggATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCaaa                                                                                                       >  1:738159/1‑141 (MQ=255)

                                        ggATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCaaa                                                                                                       <  2:541913/141‑1 (MQ=255)

                                        ggATGATGAAGTGGGATGTCGATTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGGCTCaaa                                                                                                       <  2:212826/141‑1 (MQ=255)

                                           tgatgaAGTTGTATGTCGTTTCCGTTGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCg                                                                                                    <  1:277389/141‑1 (MQ=255)

                                           tgatgaAGTTGTATGTCGTTGCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCg                                                                                                    <  1:438702/141‑1 (MQ=255)

                                               gaAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCa                                                                                                >  2:17526/1‑141 (MQ=255)

                                                 aGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCAtt                                                                                              <  2:347147/141‑1 (MQ=255)

                                                    tGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTCTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGAt                                                                                           <  1:310563/141‑1 (MQ=255)

                                                     gTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGAAAGCATGTATTCCATTCTTAGAAAATGGTGACTCAAAACGGGCAATggag                                                                                          >  2:213678/1‑138 (MQ=255)

                                                      tATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATg‑g                                                                                        >  1:556567/1‑141 (MQ=255)

                                                        tGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTAAGAAAAGGATGACTCAAACCGTGCAGTGATg‑ggg                                                                                      >  2:617392/1‑140 (MQ=255)

                                                                 cGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATg                                                                             >  1:338061/1‑141 (MQ=255)

                                                                  gTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGAGGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGAGG‑GGTGCGAAAATGc                                                                            >  2:715797/1‑141 (MQ=255)

                                                                   tGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCa                                                                           >  2:87337/1‑141 (MQ=255)

                                                                    ggTAACAATACAGTTATGGCTAAAGAAAAAATGGATTACATGGATGGAGCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCaa                                                                          <  1:258593/141‑1 (MQ=255)

                                                                          aaTACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCaa                                                                    <  1:662390/141‑1 (MQ=255)

                                                                            tACAGTGATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGc                                                                  <  2:730003/141‑1 (MQ=255)

                                                                             aCAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCa                                                                 <  1:772431/141‑1 (MQ=255)

                                                                              cAGTTATTGCGAAAGAAAAAATGGATTATATTGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGGGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAg                                                                <  2:171968/141‑1 (MQ=255)

                                                                              cAGTTATGGCTAAATAAAAAATTGATTATATGGATGTACCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAACGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAg                                                                <  1:619494/141‑1 (MQ=255)

                                                                              cAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAg                                                                <  1:272300/141‑1 (MQ=255)

                                                                              cAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAg                                                                <  2:818198/141‑1 (MQ=255)

                                                                              cAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCAGAAGGTCTTGGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAg                                                                <  1:923345/141‑1 (MQ=255)

                                                                               aGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGt                                                               <  1:828889/141‑1 (MQ=255)

                                                                               aGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGt                                                               <  1:887658/141‑1 (MQ=255)

                                                                                 ttATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGc                                                             <  2:221027/141‑1 (MQ=255)

                                                                                   aTGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCt                                                           <  1:241271/141‑1 (MQ=255)

                                                                                     gggtAAAGGAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTGAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCttt                                                         <  2:795861/138‑1 (MQ=255)

                                                                                     ggCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCttt                                                         <  1:855736/141‑1 (MQ=255)

                                                                                        tAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTtgat                                                      >  1:753795/1‑141 (MQ=255)

                                                                                        tAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTtgat                                                      >  1:492896/1‑141 (MQ=255)

                                                                                         aaaGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAGGCAGTGCCTTtgatg                                                     <  1:700666/141‑1 (MQ=255)

                                                                                         aaaGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTtgatg                                                     <  1:271046/141‑1 (MQ=255)

                                                                                         aaaGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTtgatg                                                     <  1:719164/141‑1 (MQ=255)

                                                                                              aaaaaTGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATcc                                                >  1:861529/1‑141 (MQ=255)

                                                                                              aaaaaTGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATcc                                                >  1:76233/1‑141 (MQ=255)

                                                                                                 aaTGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGa                                             >  1:158721/1‑141 (MQ=255)

                                                                                                   tGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAg                                           >  2:165193/1‑141 (MQ=255)

                                                                                                     gATTATTTGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCa                                         <  2:621974/141‑1 (MQ=255)

                                                                                                     gATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCa                                         <  2:813114/141‑1 (MQ=255)

                                                                                                      aTTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCAc                                        <  1:622651/141‑1 (MQ=255)

                                                                                                      aTTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTCCCGTTGATGAATCCAGAAGccc                                        >  1:320925/1‑139 (MQ=255)

                                                                                                       ttATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTATAAAATGATGACTCAAACCGTGCATCGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCAcc                                       <  1:608510/141‑1 (MQ=255)

                                                                                                        tataTGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGCGCGAACATGCAACGTCAAGCAGTGCCTTGGATGAATCCAGacggaaca                                      >  1:831125/1‑134 (MQ=255)

                                                                                                         atatGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCAt                                     >  1:564658/1‑141 (MQ=255)

                                                                                                         atatGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATAGCTGCACCGCG‑CTGGGCAACGTCAAACAGTGCGTATGATGAATCCAGAAGCACCAt                                     >  1:213523/1‑141 (MQ=255)

                                                                                                         atatGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCAt                                     >  1:399109/1‑141 (MQ=255)

                                                                                                              gATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGATTCCACAAGCACCATttgtt                                <  1:519660/141‑1 (MQ=255)

                                                                                                                tggaTCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTgg                              <  2:743720/138‑1 (MQ=255)

                                                                                                                    tCGCCGAAGCAAGTTGTCTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCACTTGTTGGAACa                          >  2:79130/1‑141 (MQ=255)

                                                                                                                     cGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAg                         <  2:539560/141‑1 (MQ=255)

                                                                                                                     cGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAg                         >  2:354971/1‑141 (MQ=255)

                                                                                                                      gCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAGAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAgg                        >  2:307387/1‑141 (MQ=255)

                                                                                                                      gCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAgg                        >  2:691687/1‑141 (MQ=255)

                                                                                                                      gCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCAAGAGGCACCATTTGTGGGTACAgg                        >  2:642638/1‑141 (MQ=255)

                                                                                                                      gCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTGAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAgg                        >  2:501559/1‑141 (MQ=255)

                                                                                                                       ccGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGAAGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGt                       >  1:81916/1‑141 (MQ=255)

                                                                                                                          aaGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATg                    <  1:250633/141‑1 (MQ=255)

                                                                                                                          aaGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATg                    <  2:353431/141‑1 (MQ=255)

                                                                                                                             cAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGaa                 <  2:738159/141‑1 (MQ=255)

                                                                                                                              aaGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCCTTTGTTGGTACAGGTATGGAac                >  2:334818/1‑141 (MQ=255)

                                                                                                                              aaGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGacc                >  1:887990/1‑139 (MQ=255)

                                                                                                                              aaGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGAac                <  1:21392/141‑1 (MQ=255)

                                                                                                                              aaGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGAac                <  1:476497/141‑1 (MQ=255)

                                                                                                                              aaGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGAac                >  1:396701/1‑141 (MQ=255)

                                                                                                                               aGTTGTTTCAGTAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGCacg               >  1:194205/1‑140 (MQ=255)

                                                                                                                                tctatttCAGCACCCACAGCATGTATTCTATTCTTAGAAAATCATGACTCACACCGTGCATTGATG‑GGCGCGAACATGCAACGTCAAGCAGTGCATTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGAaccc              <  2:477308/137‑3 (MQ=255)

                                                                                                                                         gcatcGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGAACACGTTgcagca     <  1:46410/141‑1 (MQ=255)

                                                                                                                                           agcGACAGCATGTATTCCATGCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGGGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGAGCACGTTGCAGCACg   >  2:876912/1‑141 (MQ=255)

                                                                                                                                            gcGACAGCATGTATTCCATTCTTAGAAAATGATGACGCAAAACGTGCGTTGACG‑GGTGCGAAGAGGCAAAGTCAAGCAGTGGCCTGCATGGATCAAGAAGGACCAGTTTTTGGGACGGGTATGGAAGCCGTTgcagccagt  >  2:298986/1‑137 (MQ=255)

CACAAGCAAACTCTAAATTAGATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATG‑GGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGAACACGTTGCAGCACGT  >  CP000730/587817‑588097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A