Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A15 F116 I1 R1	34	38.2	765948	97.1%	743735	148.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,719,269	(A)_5→6	coding (213/705 nt)	USA300HOU_2573 →	dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,719,264	1	.	A	100.0%	158.0 / NA	42	E70K (GAA→AAA)	USA300HOU_2573	dehydrogenase
Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (18/24); total (18/24)

AAAGCATTGGGTTACAAAGTTTATATAGGTTCTCGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTG‑AAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTGAAGAAGTATATCAAACGAATGTATTCGGTATCGTACGAATGATGAATACAT  >  CP000730/2719123‑2719405

aaaGCATTGGGTTACAAAGTTTATATAGGTTCTCGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTG‑aaaaaa                                                                                                                                         >  1:78562/1‑147 (MQ=255)

  aGCATTGGGTTACAAAGTTTATATAGGTTCTCGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTG‑aaaaaaga                                                                                                                                       <  2:25506/148‑4 (MQ=255)

    cATTGGGTTACAAAGTTTATATAGGTTCTCGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTG‑aaaaaagaag                                                                                                                                     >  1:76319/1‑143 (MQ=255)

    cATTGGGTTACAAAGTTTATATAGGTTCTCGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTG‑aaaaaagaag                                                                                                                                     >  1:283666/1‑143 (MQ=255)

    cATTGGGTTACAAAGTTTATATAGGTTCTCGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTG‑aaaaaagaag                                                                                                                                     >  1:268408/1‑143 (MQ=255)

          gTTACAAAGTTTATATAGGTTCTCGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTc                                                                                                                                >  2:312899/1‑148 (MQ=255)

           ttACAAAGTTTATATAGGTTCTCGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCt                                                                                                                               <  2:206121/148‑1 (MQ=255)

           ttACAAAGTTTATATAGGTTCTCGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCt                                                                                                                               <  1:214801/148‑1 (MQ=255)

           ttACAAAGTTTATATAGGTTATCGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATAATTGCAGAAAAAAGAAGGCCGTCt                                                                                                                               <  2:171222/148‑1 (MQ=255)

           ttACAAAGTTAAAATAGGCTTTCGAAATGATGTAAGAGGACAAGAAGCATCACAAAGATTAGGTGTTCAGTATGTACTATTAGATGTTACAAATGAATACAAAAGCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCt                                                                                                                               <  2:294370/148‑1 (MQ=255)

                    ttATATAGGTTCTCGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCt                                                                                                                      >  1:43166/1‑148 (MQ=255)

                    ttATATAGGTTCTCGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCt                                                                                                                      <  2:118069/148‑1 (MQ=255)

                                tcGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGc                                                                                                          <  2:251313/148‑1 (MQ=255)

                                tcGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGc                                                                                                          <  1:79959/148‑1 (MQ=255)

                                  ggAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAg                                                                                                        <  1:18815/148‑1 (MQ=255)

                                     atgatgTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGta                                                                                                     >  2:203836/1‑148 (MQ=255)

                                          gTAAGAGGACAACAAACATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCt                                                                                                <  1:155551/148‑1 (MQ=255)

                                            aaGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTgg                                                                                              <  2:137328/148‑1 (MQ=255)

                                               agGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCa                                                                                           >  2:309951/1‑148 (MQ=255)

                                                   caacaaGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAAttt                                                                                       >  1:96522/1‑148 (MQ=255)

                                                     acaaGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTc                                                                                     <  1:26466/148‑1 (MQ=255)

                                                      caaGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCa                                                                                    >  2:350547/1‑148 (MQ=255)

                                                             cacaAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCtt                                                                             >  1:341335/1‑148 (MQ=255)

                                                             cacaAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCtt                                                                             >  1:14675/1‑148 (MQ=255)

                                                                 aaaaTTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATCTCTGGTCAATTTTCAGCACCTTCCaa                                                                         >  2:303155/1‑148 (MQ=255)

                                                                 aaaaTTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCaa                                                                         >  2:291943/1‑148 (MQ=255)

                                                                 aaaaTTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCaa                                                                         >  1:98539/1‑148 (MQ=255)

                                                                    aTTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAAtt                                                                      <  2:94083/148‑1 (MQ=255)

                                                                    aTTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAAtt                                                                      <  1:133019/148‑1 (MQ=255)

                                                                    aTTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAAtt                                                                      <  1:113753/148‑1 (MQ=255)

                                                                    aTTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAAtt                                                                      <  2:358092/148‑1 (MQ=255)

                                                                    aTTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAAtt                                                                      >  2:280740/1‑148 (MQ=255)

                                                                    aTTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAAtt                                                                      >  2:250687/1‑148 (MQ=255)

                                                                         gtgtTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGacac                                                                 >  2:300536/1‑148 (MQ=255)

                                                                                ttATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGa                                                          <  1:203836/148‑1 (MQ=255)

                                                                                 tATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAACAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGAc                                                         >  2:196646/1‑148 (MQ=255)

                                                                                       cAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTgaa                                                   >  1:93313/1‑148 (MQ=255)

                                                                                       cAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCAGGAAAATTGTCAGCACCTTCCAAATTGACACCTCGTGACGTTgaa                                                   >  1:291891/1‑148 (MQ=255)

                                                                                        aattcaaTGTTAAAAGTGACTACTCAGTAAACAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTCACAATGCAGGTATATCTGGTCAATTTACAGCACCTTCCAAATTGACACCTCGTGACGTTgaag                                                  <  2:229018/142‑1 (MQ=255)

                                                                                          ttAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTgaggaa                                                <  2:259408/148‑1 (MQ=255)

                                                                                          ttAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTgaagaa                                                <  1:128066/148‑1 (MQ=255)

                                                                                            aGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTGAAGAAGt                                              >  2:135154/1‑148 (MQ=255)

                                                                                                  tACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTGAAGAAGTATATCa                                        <  1:308293/148‑1 (MQ=255)

                                                                                                  tACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTGAAGAAGTATATCa                                        <  2:106897/148‑1 (MQ=255)

                                                                                                                          tGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTGAAGAAGTATATCAAACGAATGTATTCGGTATCGTACg                <  1:309951/148‑1 (MQ=255)

                                                                                                                              tataACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTGAAGAAGTATATCAAACGAATGTATTCGGTATCGTACGAatg            <  1:350547/148‑1 (MQ=255)

                                                                                                                                        ttGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTGAAGAAGTATATCAAACGAATGTATTCGGTATCGTACGAATGATGAATACAt  <  2:78562/148‑1 (MQ=255)

AAAGCATTGGGTTACAAAGTTTATATAGGTTCTCGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTG‑AAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTGAAGAAGTATATCAAACGAATGTATTCGGTATCGTACGAATGATGAATACAT  >  CP000730/2719123‑2719405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A