Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F121 I1 R1	25	27.5	612106	95.4%	583949	136.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	39,246	C→T	A630T (GCT→ACT)	mecA ←	penicillin binding protein 2 prime

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	39,246	0	C	T	100.0%	67.6 / NA	22	A630T (GCT→ACT)	mecA	penicillin binding protein 2 prime
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (13/9); total (13/9)

CTGTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATCTTCT  >  CP000730/39119‑39382

cTGTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGttt                                                                                                                             >  1:226509/1‑141 (MQ=255)

    tttGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGAt                                                                                                                         <  2:88091/141‑1 (MQ=255)

       gTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTAt                                                                                                                      <  2:90360/141‑1 (MQ=255)

                   tatCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCat                                                                                                             >  2:182872/1‑138 (MQ=255)

                   tatCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCat                                                                                                             <  1:182872/138‑1 (MQ=255)

                                         ctcATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCt                                                                                    >  2:188479/1‑141 (MQ=255)

                                             tataGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCa                                                                                >  2:155439/1‑141 (MQ=255)

                                              ataGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAg                                                                               <  2:274698/141‑1 (MQ=255)

                                              ataGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAg                                                                               <  2:88870/141‑1 (MQ=255)

                                                    catcatACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTtctc                                                                         >  2:243375/1‑141 (MQ=255)

                                                      tcatACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATACGTTTATCTTGTCCATATTTAACATCAAACGTCTTCTTTATCGTTGTATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCt                                                                       <  1:258186/141‑1 (MQ=255)

                                                              tttACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCAt                                                               >  2:266099/1‑141 (MQ=255)

                                                                  ccTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCATTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTGGTCTGCCAGTTTTTCCTTGTTTCAttttt                                                           >  2:209705/1‑140 (MQ=255)

                                                                      agaTTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGtt                                                       >  1:129287/1‑141 (MQ=255)

                                                                                         agcCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGcc                                    <  1:40465/141‑1 (MQ=255)

                                                                                              ttCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTa                               <  2:160743/141‑1 (MQ=255)

                                                                                                          gTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAg                   >  1:207295/1‑141 (MQ=255)

                                                                                                          gTACATCTTTAACATTAATAGTCAGCATCATGGTTGGGTTATCTTTATCATATGATATTAACCACTCAATTTGTATGTTAGTTTCACCGTGTTTCATCTTTAGGTCTGCATGATCGGAATTGCCATTTAAGTTTTCAttaa                   >  2:49247/1‑138 (MQ=255)

                                                                                                                          aaTAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATAtcttc   >  1:136093/1‑141 (MQ=255)

                                                                                                                           aTAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAatat       >  1:305065/1‑136 (MQ=255)

                                                                                                                           aTAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGATTCATTTTAAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATAtcttct  >  1:254612/1‑141 (MQ=255)

                                                                                                                           aTAGTCATCATCATGATTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAatat       <  2:305065/136‑1 (MQ=255)

CTGTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATCTTCT  >  CP000730/39119‑39382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A