Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I1 R1 14 24.7 1576304 96.5% 1521133 75.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA REL606 1,329,516 C→T H33Y (CAC→TAC)  topA → DNA topoisomerase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6061,329,5160CT100.0% 54.2 / NA 17H33Y (CAC→TAC) topADNA topoisomerase I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/9);  total (8/9)

TCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCT  >  REL606/1329447‑1329587
                                                                     |                                                                       
tCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATcc                                                                   <  2:60313/76‑1 (MQ=255)
               aaaaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCa                                                    >  1:493046/1‑76 (MQ=255)
                    naTCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGc                                               <  1:669139/75‑1 (MQ=255)
                    gATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGc                                               >  2:493824/1‑76 (MQ=255)
                       caacaaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCa                                            >  2:159387/1‑76 (MQ=255)
                        aacaaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAg                                           <  2:231053/76‑1 (MQ=255)
                          caaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCt                                         >  2:449430/1‑76 (MQ=255)
                              tATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCa                                     <  1:965/76‑1 (MQ=255)
                                    ggTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAaga                               >  2:484257/1‑76 (MQ=255)
                                     gTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAagag                              <  2:456126/76‑1 (MQ=255)
                                               cGTGGTGAAATCCAGCGTCGTNTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGActct                    <  2:662316/76‑1 (MQ=255)
                                                gtggtgAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTa                   >  1:347961/1‑76 (MQ=255)
                                                     gacaTCCAGCGTCGGTTACATCCGCGNTTTGCCAACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACGTcc              >  2:679375/4‑76 (MQ=255)
                                                     gAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTcc              >  2:602343/1‑76 (MQ=255)
                                                     gAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTcc              <  1:268510/76‑1 (MQ=255)
                                                          ccAGCGTCGGTTACCTCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCaa         <  2:519816/76‑1 (MQ=255)
                                                                 cGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCt  <  1:181273/76‑1 (MQ=255)
                                                                     |                                                                       
TCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCT  >  REL606/1329447‑1329587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A