Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A28 F1 I1 R1
|
15 |
45.6 |
2893554 |
96.7% |
2798066 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
1,329,516 |
C→T |
H33Y (CAC→TAC) |
topA → |
DNA topoisomerase I |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 1,329,516 | 0 | C | T | 100.0%
| 114.5
/ NA
| 31 | H33Y (CAC→TAC) | topA | DNA topoisomerase I |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (19/12); total (19/12) |
TGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTA > REL606/1329443‑1329588
|
tGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTtac > 1:994748/1‑76 (MQ=255)
gAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTtaca < 2:607001/76‑1 (MQ=255)
aGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAATGACTACGTGGTGAAATCCAGCGTCGGTTACAt < 2:1230786/76‑1 (MQ=255)
tCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATcc > 1:1281155/1‑76 (MQ=255)
tCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATcc > 2:1109441/1‑76 (MQ=255)
ccccGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCcg < 2:369868/76‑1 (MQ=255)
aaaaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTg > 2:1277191/1‑76 (MQ=255)
aaaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGc > 2:1415602/1‑76 (MQ=255)
aaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGcc > 2:1009517/1‑76 (MQ=255)
aaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCAATTTGca > 2:1373744/1‑75 (MQ=255)
cGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTgg > 1:829740/1‑76 (MQ=255)
caacaaGTATCTGGGTAGTGACTACGTNGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCa > 1:1433610/1‑76 (MQ=255)
aacaaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAg < 1:1029810/76‑1 (MQ=255)
acaaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGc < 1:484303/76‑1 (MQ=255)
tATCCGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCa > 2:1419842/1‑76 (MQ=255)
gggTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAag > 2:314952/1‑76 (MQ=255)
tGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGcc > 2:483918/1‑76 (MQ=255)
tGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGANGANCAGTGGCTCAGCTGNCAAAAAGAGTGcc > 2:1304061/1‑76 (MQ=255)
gACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCg < 2:988014/76‑1 (MQ=255)
gACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCg > 1:902916/1‑76 (MQ=255)
cTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCANAAAGAGTGcctgc > 1:190658/1‑73 (MQ=255)
cTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGAc < 1:150099/76‑1 (MQ=255)
aTCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTccacc < 1:1106599/76‑1 (MQ=255)
aTCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCCCAGCTGCCAAAAAGAGTGCCGACTCTACCTccacc < 1:66952/76‑1 (MQ=255)
ccAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCATAAAGAGTGCCGACTCTACCTCCACCaa < 1:1429250/76‑1 (MQ=255)
ccAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCaa < 2:1134471/76‑1 (MQ=255)
ccAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCaa < 1:362472/76‑1 (MQ=255)
ccAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCaa < 1:247611/76‑1 (MQ=255)
cAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAg > 2:894102/1‑76 (MQ=255)
cAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAg > 1:1159103/1‑76 (MQ=255)
cAGCGTCGGTTACACCCGCGATTTACCGGCCNGTGGCTCACCTGCCNAAAAGNGTGCCGACTCTACCTCCACCAAg < 2:190652/76‑1 (MQ=255)
aGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGa < 2:642061/76‑1 (MQ=255)
cGTCGGATACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAATAGTGCCGACTCTACCTCCACCAAGACg < 1:1419842/76‑1 (MQ=255)
cGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTNTACCTCCACCAAGACGGCt < 1:1340398/76‑1 (MQ=255)
cGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCt > 2:422026/1‑76 (MQ=255)
cGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCt > 1:1420911/1‑76 (MQ=255)
ggTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTa > 1:1064681/1‑76 (MQ=255)
|
TGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTA > REL606/1329443‑1329588
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
