Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F1 I1 R1
|
15 |
35.6 |
2322208 |
94.1% |
2185197 |
75.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
1,329,516 |
C→T |
H33Y (CAC→TAC) |
topA → |
DNA topoisomerase I |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 1,329,516 | 0 | C | T | 100.0%
| 68.8
/ NA
| 22 | H33Y (CAC→TAC) | topA | DNA topoisomerase I |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (12/10); total (12/10) |
TGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCT > REL606/1329443‑1329587
|
tGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTtac < 1:797495/76‑1 (MQ=255)
tGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTaac > 1:546008/1‑76 (MQ=255)
cGGCAAAAGCCAAAACGATCATCAAGTATCTNGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGa > 1:102147/1‑76 (MQ=255)
gCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGAGTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGAtt < 2:350775/76‑1 (MQ=255)
cAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGAttt < 2:1137944/76‑1 (MQ=255)
aaaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGc > 2:148429/1‑76 (MQ=255)
gCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGa > 2:344620/1‑76 (MQ=255)
aaaaCGATCAACAAGTATCTGGGTNGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCa > 2:201925/1‑76 (MQ=255)
aaaaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCTGACCa > 2:939373/1‑76 (MQ=255)
aaaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAg < 2:739389/76‑1 (MQ=255)
aCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTg < 2:426044/76‑1 (MQ=255)
aTCAACAAGTATCTGGGTCGTGACTACGTGGTGAAATCCAGCGTCGGATACATCCGCGATTTGCCGACCAGTGGCt > 2:1051459/1‑76 (MQ=255)
aTCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCt < 1:158270/76‑1 (MQ=255)
aTCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCt < 2:641934/76‑1 (MQ=255)
gTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGcc > 1:605532/1‑76 (MQ=255)
tATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCa > 1:1144451/1‑76 (MQ=255)
aTCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCaa > 2:366755/1‑76 (MQ=255)
aTCTGGGTAGTGACTACGTGGTGAAAGCCAGCGNCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCaa < 1:929296/76‑1 (MQ=255)
cTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCaaaa > 1:330624/1‑76 (MQ=255)
gTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAagag > 1:284810/1‑76 (MQ=255)
tgAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTc < 1:68893/76‑1 (MQ=255)
aGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAGAGAGTGCCGACTCTACCTCCACCACGa > 1:550852/1‑76 (MQ=255)
gCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGAc < 2:471195/76‑1 (MQ=255)
gCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGAc < 2:631843/76‑1 (MQ=255)
cGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGCGTGCCGACTCTACCTCCACCAAGACGGCt < 1:1135284/76‑1 (MQ=255)
|
TGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCT > REL606/1329443‑1329587
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
