Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F1 I1 R1
|
17 |
44.3 |
2973330 |
95.1% |
2827636 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
1,329,516 |
C→T |
H33Y (CAC→TAC) |
topA → |
DNA topoisomerase I |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 1,329,516 | 0 | C | T | 100.0%
| 114.9
/ NA
| 33 | H33Y (CAC→TAC) | topA | DNA topoisomerase I |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (19/14); total (19/14) |
CCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTA > REL606/1329450‑1329588
|
ccGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCcgcg < 2:1114127/76‑1 (MQ=255)
gCAAAAGCCATAACGATCTACAAGTATCTGGGTTGTGACNNCGTGGTGNAATCCTGCGTCGGTTTCATCCGCGAtt < 1:1454992/76‑1 (MQ=255)
gCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGAtt > 2:301791/1‑76 (MQ=255)
aaaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGc < 1:167565/76‑1 (MQ=255)
aaGCCAAAACGATCNACAAGTATCAGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGcc > 1:1622/1‑76 (MQ=255)
aaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTCGTGAAATCCAGCGTCGGTTACATCCGCGATTTGcc > 2:1179735/1‑76 (MQ=255)
aaGCCAAAACGATCAACAAATATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGcc > 1:1255293/1‑76 (MQ=255)
aGCCAAAACGATCAACAAGTANCTNGGTAGTGACTACGTGGTGAAANCCAGCGTNGNTTACATCCGCGATTTGCCg > 2:221501/1‑76 (MQ=255)
cAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGAcc < 2:1396294/76‑1 (MQ=255)
aaaaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCa < 2:1317476/76‑1 (MQ=255)
aaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGt > 2:763003/1‑76 (MQ=255)
aacaaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAg < 1:964663/76‑1 (MQ=255)
acaaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGc > 2:89854/1‑76 (MQ=255)
aaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTg > 1:300019/1‑76 (MQ=255)
gTATCGGGGTAGGGGCTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGcc < 2:468881/76‑1 (MQ=255)
tATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCa > 1:858656/1‑76 (MQ=255)
tATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCa > 2:604682/1‑76 (MQ=255)
aGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTCGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTg > 2:189233/1‑76 (MQ=255)
aCGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGActc > 2:415806/1‑76 (MQ=255)
aCGTGGTGAAATCCAGCGTCAGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGActc > 2:128875/1‑76 (MQ=255)
cGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGActct > 2:767191/1‑76 (MQ=255)
tgggtgAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTa < 2:808439/74‑1 (MQ=255)
gtgAAATCCAGCGTTGGGTGCAACCGCGATTTGCCGACCAGTGGCTCAGCTCCCACAAAGAGTGCCGACTCTACCt < 1:72384/76‑1 (MQ=255)
gtgAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCt < 1:682034/76‑1 (MQ=255)
tgAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTc < 2:951744/76‑1 (MQ=255)
gAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTcc > 1:1112240/1‑76 (MQ=255)
aaaTCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTTTTCCTcca < 1:1374249/76‑1 (MQ=255)
aaTCCAGCGGCGGTTACCTCCGCGAATTGTCGACCAGTGNCTCAGGTGCCAAAAANAGTGCCGACTCTACCTccac > 2:1475015/1‑76 (MQ=255)
tCCAGCGTNGGTTACATCCGCGATTTGACGACCGGGGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTccacca < 2:249580/76‑1 (MQ=255)
ccAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCaa > 1:512596/1‑76 (MQ=255)
aGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCCCCAAGa > 1:990083/1‑76 (MQ=255)
cGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACg > 1:1014909/1‑76 (MQ=255)
ggTTACATNCGCGATTTGCCGACCAGTGGCTCATGCGCCAAAAAGAGTGCCGTCTCTACCTCCACCAAGACGGCTa < 2:248594/76‑1 (MQ=255)
ggTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTa < 2:197889/76‑1 (MQ=255)
|
CCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTA > REL606/1329450‑1329588
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
