Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A48 F1 I1 R1
|
19 |
65.6 |
4133022 |
97.0% |
4009031 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| JC |
REL606 |
1,881,180 |
Δ10 bp |
coding (554‑563/972 nt) |
manX → |
fused mannose‑specific PTS enzymes: IIA component/IIB component |
|
| |
seq id |
position |
reads (cov) |
reads (cov) |
score |
skew |
freq |
annotation |
gene |
product |
| * |
? |
REL606 |
= 1881179 | 3 (0.050) | 54 (0.830) |
44/150 |
0.4 |
94.7% |
coding (553/972 nt) |
manX |
fused mannose‑specific PTS enzymes: IIA component/IIB component |
| ? | REL606 |
1881190 = |
3 (0.050) | coding (564/972 nt) |
manX |
fused mannose‑specific PTS enzymes: IIA component/IIB component |
TACATGGTTATTGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ > REL606/1881107‑1881179
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTCCCGTATTATTGTTGTTAGTGATGAAGTGGCTGCGGATACCGTTCGTAAGACACTGCTCACCCAGGTTGCA > REL606/1881190‑1881262
TACATGGTTATTGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGCTC < 1:611215/76‑1
TACATGGTTATTGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGCTC > 1:391520/1‑76
ACATGGTTATTGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGCTCC > 1:986034/1‑76
ACATGGTTATTGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGCTCC > 1:865657/1‑76
CATGGTTATTGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGCTCCC > 2:206448/1‑76
ATGGTTATTGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGCTCCCG > 1:1744885/1‑76
TGGTTATTGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGCTCCCGT < 2:470340/76‑1
TTATTGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGCTCCCGTATT > 2:1349204/1‑76
TTATTGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGCTCCCGTATT > 2:1173832/1‑76
TTATTGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGCTCCCGTATT < 2:1748466/76‑1
TATTGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGCTCCCGTATca > 2:496211/1‑74
TATTGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGCTCCCGTATTA > 1:622595/1‑76
TATTGGCCTTGCGCGTATCGACGACCGTCTGATTCAC‑GTCAGGTCGCCACCCGCTGGACGAAAGCTCCCGTATTAT < 1:472436/76‑1
TGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGCTCCCGTATTATTG < 2:464025/76‑1
CCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGCTCCCGTATTATTGTTG > 2:1290300/1‑76
CTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGCTCCCGTATTATTGTTGT < 2:1749227/76‑1
CTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGCTCCCGTATTATTGTTGT > 2:81629/1‑76
CTTGCGCGTATCGACGACCGTCTGATTCACGGCAAGGTCGCCACCCGCTGGACCAAAGCTCCCGTATTATTGTTGT > 2:779805/1‑76
TGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGCTCCCGTATTATTGTTGTTA > 1:490574/1‑76
CGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGCTCCCGTATTATTGTTGTTAGTGA > 1:853281/1‑76
GTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGCTCCCGTATTATTGTTGTTAGTGAT > 1:1370092/1‑76
CGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGCTCCCGTATTATTGTTGTTAGTGATGAAGTGG > 2:645634/1‑76
GACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGCTCCCGTATTATTGTTGTTAGTGATGAAGTGGC > 2:1644193/1‑76
GACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGCTCCCGTATTATTGTTGTTAGTGATGAAGTGGC < 2:2023278/76‑1
GACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGCTCCCGTATTATTGTTGTTAGTGATGAAGTGGC > 2:751608/1‑76
nCCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGCTCCCGTATTATTGTTGTTAGTGATGAAGTGGCT > 1:272620/2‑76
CGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGCTCCCGTATTATTGTTGTTAGTGATGAAGTGGCTGC < 2:606885/76‑1
CTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGCTCCCGTATTATTGTTGTTAGTGATGAAGTGGCTGCGGA > 1:1143604/1‑76
TGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGCTCCCGTATTATTGTTGTTAGTGATGAAGTGGCTGCGGAT < 2:227633/76‑1
TGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGCTCCCGTATTATTGTTGTTAGTGATGAAGTGGCTGCGGAT > 2:198733/1‑76
ACGGTCAGGTCGCCACCCGCTGGACCAAAGCTCCCGTATTATTGTTGTTAGTGATGAAGTGGCTGCGGATACCGTT > 1:1358020/1‑76
CGGTCAGGTCGCCACCCGCTGGACCAAAGCTCCCGTATTATTGTTGTTAGTGATGAAGTGGCTGCGGATACCGTTC < 1:645873/76‑1
GGTCAGGTCGCCACCCGCTGGACCAAAGCTCCCGTATTATTGTTGTTAGTGATGAAGTGGCTGCGGATACCGTTCG < 2:503128/76‑1
AGGTCGCCACCCGCTGGACCAAAGCTCCCGTATTATTGTTGTTAGTGATGAAGTGGCTGCGGATACCGTTCGTAAG < 1:1655508/76‑1
AGGTCGCCACCCGCTGGACCAAAGCTCCCGTATTATTGTTGTTAGTGATGAAGTGGCTGCGGATACCGTTCGTAAG > 2:1570711/1‑76
GGTCGCCACCCGCTGGACCAAAGCTCCCGTATTATTGTTGTTAGTGATGAAGTGGCTGCGGATACCGTTCGTAAGA > 1:1956994/1‑76
GTCGCCACCCGCTGGACCAAAGCTCCCGTATTATTGTTGTTAGTGATGAAGTGGCTGCGGATACCGTTCGTAAGAC < 2:1441369/76‑1
CGCCACCCGCTGGACCAAAGCTCCCGTATTATTGTTGTTAGTGATGAAGTGGCTGCGGATACCGTTCGTAAGACAC > 1:1518506/1‑76
CACCCGCTGGACCAAAGCTCCCGTATTATTGTTGTTAGTGATGAAGTGGCTGCGGATACCGTTCGTAAGACACTGC > 2:487208/1‑76
ACCCGCTGGACCAAAGCTCCCGTATTATTGTTGTTAGTGATGAAGTGGCTGCGGATACCGTTCGTAAGACACTGCT > 2:1759258/1‑76
CCGCTGGACCAAAGCTCCCGTATTATTGTTGTTAGTGATGAAGTGGCTGCGGATACCGTTCGTAAGACACTGCTCA > 2:146228/1‑76
CGCTGGACCAAAGCTCCCGTATTATTGTTGTTAGTGATGAAGTGGCTGCGGATACCGTTCGTAAGACACTGCTCAC < 1:1699175/76‑1
CTGGACCAAAGCTCCCGTATTATTGTTGTTAGTGATGAAGTGGCTGCGGATACCGTTCGTAAGACACTGCTCACCC > 1:1967178/1‑76
TGGACCAAAGCTCCCGTATTATTGTTGTTAGTGATGAAGTGGCTGCGGATACCGTTCGTAAGACACTGCTCACCCA > 1:12384/1‑76
TGGACCAAAGCTCCCGTATTATTGTTGTTAGTGATGAAGTGGCTGCGGATACCGTTCGTAAGACACTGCTCACCCA > 2:1665469/1‑76
TGGACCAAAGCTCCCGTATTATTGTTGTTAGTGATGAAGTGGCTGCGGATACCGTTCGTAAGACAACGCTCACCCA > 2:306567/1‑76
GGACCAAAGCTCCCGTATTATTGTTGTTAGTGATGAAGTGGCTGCGGATACCGTTCGTAAGACACTGCTCACCCAG > 2:1229415/1‑76
GACCAAAGCTCCCGTATTATTGTTGTTAGTGATGAAGTGGCTGCGGATACCGCGCGTAAGACACTGCTCCCCCAGG > 2:794632/1‑76
CCAAAGCTCCCGTATTATTGTTGTTAGTGATGAAGTGGCTGCGGATACCGTTCGTAAGACACTGCTCACCCAGGTT < 1:1783562/76‑1
CAAAGCTCCCGTATTATTGTTGTTAGTGATGAAGTGGCTGCGGATACCGTTCGTAAGACACTGCTCACCCAGGTTG < 1:598751/76‑1
CAAAGCTCCCGTATTATTGTTGTTAGTGATGAAGTGGCTGCGGATACCGTTCGTAAGACACTGCTCACCCAGGTTG < 1:1485163/76‑1
CAAAGCTCCCGTATTATTGTTGTTAGTGATGAAGTGGCTGCGGATACCGTTCGTAAGACACTGCTCACCCAGGCTG > 2:871179/1‑76
AAGCTCCCGTATTATTGTTGTTAGTGATGAAGTGGCTGCGGATACCGTTCGTAAGACACTGCTCACCCCGGTTGCA > 1:1290648/1‑76
AAGCTCCCGTATTATTGTTGTTAGTGATGAAGTGGCTGCGGATACCGTTCGTAAGACACTGCTCACCCAGGTTGCA > 2:1504363/1‑76
TACATGGTTATTGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ > REL606/1881107‑1881179
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTCCCGTATTATTGTTGTTAGTGATGAAGTGGCTGCGGATACCGTTCGTAAGACACTGCTCACCCAGGTTGCA > REL606/1881190‑1881262
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
|---|
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |
GATK/CNVnator alignment
N/A
