Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A138 F1 I1 R1 17 163.5 10262604 95.7% 9821312 76.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
JC REL606 2,103,918 (CCAG)7→8 coding (185/216 nt) ECB_01992 → hypothetical protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 = 21038870 (0.000)45 (0.300)
+CAGC		 27/80 2.7 100% coding (154/216 nt) ECB_01992 hypothetical protein
?REL606 2103888 = 0 (0.000)coding (155/216 nt) ECB_01992 hypothetical protein

AAACATGGCTATACAATGAAAACTGCTTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/2103813‑2103887
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGATTTTGTACTACGAAATAATTGCCCTTATTAT  >  REL606/2103888‑2103962
                                                                           |||||                                                                            
AAACATGGCTATACAATGAAAACTGCTTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCAC                                                                                  <  1:3269471/76‑1
AAACATGGCTATACAATGAAAACTGCTTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCAC                                                                                  >  2:1724728/1‑76
AAACATGGCTATACAATGAAAACTGCTTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCAC                                                                                  >  1:2709999/1‑76
  ACATGGCTATACAATGAAAACTGCTTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG                                                                                <  1:3541633/76‑1
  ACATGGCTATACAATGAAAACTGCTTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG                                                                                <  2:5021884/76‑1
  ACATGGCTATACAATGAAAACTGCTTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG                                                                                >  1:1398317/1‑76
   CATGGCTATACAATGAAAACTGCTTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑C                                                                              >  1:1264321/1‑76
   CATGGCTATACAATGAAAACTGCTTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑C                                                                              <  1:2879032/76‑1
     TGGCTATACAATGAAAACTGCTTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCA                                                                            <  1:3289208/76‑1
      GGCTATACAATGAAAACTGCTTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG                                                                           <  1:771759/76‑1
       GCTCTACAATGAAAACTGCTTCGTTATTGACTTCGAATATAAAGGGGCGAAATTTGCATTAGATTTAACAG‑CCAG‑C                                                                         >  2:263999/1‑76
       GCTATACAATGAAAACTGCTTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑C                                                                         >  1:1173363/1‑76
        CTATACAATGAAAACTGCTTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CC                                                                        <  1:2708202/76‑1
         TATACAATGAAAACTGCTTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCA                                                                       <  2:2912870/76‑1
         TATACAATGAAAACTGCTTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCA                                                                       >  1:3147667/1‑76
         TATACAATGAAAACTGCTTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCA                                                                       <  2:1092947/76‑1
          ATACAATGAAAACTGCTTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAG                                                                      <  1:2225924/76‑1
          ATACAATGAAAACTGCTTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAG                                                                      >  1:3792428/1‑76
           TACAATGAAAACTGCTTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAGC                                                                     >  1:605190/1‑76
            ACAATGAAAACTGCTTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAGCC                                                                    >  2:3770940/1‑76
             CAATGAAAACTGCTTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAGCCA                                                                   >  2:3069000/1‑76
              AATGAAAACTGCTTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAGCCAG                                                                  >  1:3086476/1‑76
              AATGAAAACTGCTTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAGCCAG                                                                  <  1:1652890/76‑1
              AATGAAAACTGCTTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CAAG‑CCAGCCAG                                                                  >  1:2209080/1‑76
               ATGAAAACTGCTTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAGCCAGC                                                                 >  2:3073690/1‑76
                TGAAAACTGCTTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAGCCAGCC                                                                <  2:3585824/76‑1
                  AAAACTGCTTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAGCCAGCCAG                                                              >  2:2747000/1‑76
                  AAAACTGCTTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAGCCAGCCAG                                                              <  1:3734313/76‑1
                  AAAACTGCTTCGTTATTGACTTCGAATATAAAGGGGTAAAATTTGCATTAGATTTCACAG‑CCAG‑CCAGCCAGCCAG                                                              <  2:4484947/76‑1
                     ACTGCTTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGTTTTCACAG‑CCAG‑CCAGCCAGCCAGCCA                                                           <  1:2372481/76‑1
                         CTTCTTTATTGACTTCGAATAAAAGGGGGTGAATTTTGCATTAAATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCA                                                       <  1:1018141/76‑1
                         CTTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCA                                                       >  1:4830421/1‑76
                         CTTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCA                                                       <  1:844290/76‑1
                         CTTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCA                                                       <  1:5029547/76‑1
                          TTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAG                                                      >  1:2729740/1‑76
                            CGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCC                                                    >  1:58030/1‑76
                               TATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGC                                                 >  1:435941/1‑76
                                ATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCC                                                >  1:417156/1‑76
                                ATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCC                                                >  1:2911690/1‑76
                                 TTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCA                                               >  1:4016823/1‑76
                                  TGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGACAG                                              >  1:1794287/1‑76
                                    ACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGT                                            >  2:230450/1‑76
                                     CTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTG                                           >  2:513781/1‑76
                                      TTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGT                                          >  2:3189164/1‑76
                                       TCGAATATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTG                                         >  2:3205442/1‑76
                                            TATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGT                                    >  2:3541791/1‑76
                                            TATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGT                                    >  2:1437467/1‑76
                                             ATAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTG                                   >  1:4997836/1‑76
                                              TAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGG                                  >  2:2929031/1‑76
                                              TAAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGG                                  >  2:1443633/1‑76
                                               AAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGA                                 >  1:4038823/1‑76
                                               AAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGA                                 >  2:485500/1‑76
                                               AAAGGGGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGA                                 >  1:4562360/1‑76
                                                    GGTGAAATTTGCATTAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGATTTTG                            >  2:48304/1‑76
                                                        AAATTTGCATTAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGAGTGAATGTTGATTTTGTACT                        >  2:2689426/1‑76
                                                          ATTTGCATTAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGATTTTGTACTAC                      >  1:4577624/1‑76
                                                           TTTGCATTAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGATTTTGTACTACG                     >  2:3498552/1‑76
                                                              GCATTAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGATTTTGTACTACGAAA                  >  1:2942993/1‑76
                                                               CATTAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGATTTTGTACTACGAAAT                 >  1:699995/1‑76
                                                                ATTAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGATTTTGTACTACGAAATA                >  2:1512808/1‑76
                                                                 TTAGATTTCCCCG‑CCCGCCCACCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGATTTTGTACTACGAAATA                <  1:1833604/76‑1
                                                                 TTAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGATTTTGTACTACGAAATAA               >  1:1876437/1‑76
                                                                 TTAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGATTTTGTACTACGAAATAA               >  2:4860394/1‑76
                                                                 TTAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGATTTTGTACTACGAAATAA               >  2:2700662/1‑76
                                                                 TTAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGATTTTGTACTACGAAATAA               >  2:1500322/1‑76
                                                                  taaATTTCACAGCCCAC‑CCAGCCGGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGATTTTGTACTACGAAATAA               <  2:2866140/73‑1
                                                                  TAGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGATTTTGTACTACGAAATAAT              >  1:3512633/1‑76
                                                                   AGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGATTTTGTACTACGAAATAATT             >  1:3992364/1‑76
                                                                   AGATTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGATTTTGTACTACGAAATAATT             >  2:477989/1‑76
                                                                      TTTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGATTTTGTACTACGAAATAATTGCC          >  1:1319238/1‑76
                                                                       TTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGATTTTGTACTACGCAATAATTGCCC         >  1:3673894/1‑76
                                                                       TTCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGATTTTGTACTACGAAATAATTGCCC         >  2:3628738/1‑76
                                                                        TCACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGATTTTGTACTACGAAATAATTGCCCT        >  1:1798588/1‑76
                                                                         CACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGATTTTGTACTACGAAATAATTGCCCTT       >  1:4363621/1‑76
                                                                         CACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGATTTTGTACTACGAAATAATTGCCCTT       >  1:2375925/1‑76
                                                                         CACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGATTTTGTACTACGAAATAATTGCCCTT       >  1:3271580/1‑76
                                                                         CACAC‑CCAC‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGATTTTGTACTACGAAATAATTGCCCTT       <  2:5123620/76‑1
                                                                          ACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGATTTTGTACTACGAAATAATTGCCCTTA      >  2:4464927/1‑76
                                                                          ACAG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGATTTTGTACTACGAAATAATTGCCCTTA      >  2:482685/1‑76
                                                                            AG‑CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGATTTTGTACTACGAAATAATTGCCCTTATT    >  2:3197661/1‑76
                                                                              cCCAC‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGATTTTGTACTACGAAATAATTGCCCTTATTA   <  1:2554879/75‑1
                                                                               CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGATTTTGTACTACGAAATAATTGCCCTTATTAT  >  1:1704249/1‑76
                                                                               CCAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGATTTTGTACTACGAAATAATTGCCCTTATTAT  >  1:3868447/1‑76
                                                                           |||||                                                                            
AAACATGGCTATACAATGAAAACTGCTTCGTTATTGACTTCGAATATAAAGGGGTGAAATTTGCATTAGATTTCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/2103813‑2103887
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CAG‑CCAGCCAGCCAGCCAGCCAGCCAGCCAGGTGTGAATGTGGATTTTGTACTACGAAATAATTGCCCTTATTAT  >  REL606/2103888‑2103962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.

GATK/CNVnator alignment

N/A