Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I52 R1 239 220.4 11079197 93.2% 10325811 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,749,326 A→C D32A (GAT→GCT)  sgbU → putative L‑xylulose 5‑phosphate 3‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,749,3260AC99.6% 816.6 / NA 224D32A (GAT→GCT) sgbUputative L‑xylulose 5‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  major base C (93/129);  minor base T (1/0);  total (94/130)
Fisher's exact test for biased strand distribution p-value = 4.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 224 total aligned reads displayed.

CGCGTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCG  >  NC_000913/3749229‑3749421
                                                                                                 |                                                                                               
cgcgTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCtt                                                                                               <  2:21922/100‑1 (MQ=255)
cgcgTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCtt                                                                                               <  1:4531742/100‑1 (MQ=255)
   gtgcgtAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTg                                                                                            <  2:3267759/100‑1 (MQ=255)
   gtgcgtAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTg                                                                                            <  2:3131622/100‑1 (MQ=255)
   gtgcgtAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTg                                                                                            >  2:877070/1‑100 (MQ=255)
   gtgcgtAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTg                                                                                            <  1:2587853/100‑1 (MQ=255)
    tgcgtAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGt                                                                                           >  2:1736953/1‑100 (MQ=255)
    tgcggAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGt                                                                                           <  2:4595097/100‑1 (MQ=255)
      cgtAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCg                                                                                         >  2:1692476/1‑100 (MQ=255)
      cgtAATCATCAGTTAAAAAAAAAAGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCg                                                                                         <  2:542940/100‑1 (MQ=255)
        tAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCGTTTGTCGaa                                                                                       >  2:4764918/1‑100 (MQ=255)
         aaTCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGaaa                                                                                      <  1:1291572/100‑1 (MQ=255)
          atcatcAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAAt                                                                                     <  1:5501613/100‑1 (MQ=255)
          atcatcAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAAt                                                                                     <  1:414782/100‑1 (MQ=255)
            catcaGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGt                                                                                   >  1:97735/1‑100 (MQ=255)
             ttcaGTTAGGGAGTTATGAAAAAGCGCTGGCGAAAGAGATCGCCTGGCCGTCGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTc                                                                                  <  2:4241395/99‑1 (MQ=255)
                aGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGt                                                                               >  2:1278473/1‑100 (MQ=255)
                aGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGt                                                                               >  1:704366/1‑100 (MQ=255)
                aGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGt                                                                               >  1:1567099/1‑100 (MQ=255)
                  tctgggATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTgg                                                                             <  2:1491729/97‑1 (MQ=255)
                   tAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGa                                                                            >  1:3166959/1‑100 (MQ=255)
                   tAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGAAAAAAAAGAAAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGa                                                                            <  2:1827880/100‑1 (MQ=255)
                     gggATTTATGAAAAAGCGCTGGCGAAAGATCTCTGCTGGCCGGAGCGGCTGAAAAAAAAAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATg                                                                          <  2:923148/100‑1 (MQ=255)
                     gggATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATg                                                                          >  2:2146671/1‑100 (MQ=255)
                     gggATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATg                                                                          <  2:5132978/100‑1 (MQ=255)
                     gggATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATg                                                                          <  2:307400/100‑1 (MQ=255)
                       gATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGaa                                                                        >  1:173208/1‑100 (MQ=255)
                       gATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGaa                                                                        >  1:4037063/1‑100 (MQ=255)
                         tttATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAAc                                                                      <  2:3469433/100‑1 (MQ=255)
                         tttATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAAc                                                                      <  1:1997450/100‑1 (MQ=255)
                           tATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACcg                                                                    >  1:2971881/1‑100 (MQ=255)
                           tATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACcg                                                                    <  1:1628251/100‑1 (MQ=255)
                            aTGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACcga                                                                   <  1:2106345/100‑1 (MQ=255)
                              gAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACcgacg                                                                 >  2:816345/1‑100 (MQ=255)
                              gAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACcgacg                                                                 <  2:3873775/100‑1 (MQ=255)
                                aaaaGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTGTTGTCGAAATGTCGGTGGATGCAACCGACGaa                                                               >  1:5103967/1‑100 (MQ=255)
                                 aaaGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAAc                                                              >  1:4300065/1‑100 (MQ=255)
                                 aaaGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAAc                                                              <  1:3769844/100‑1 (MQ=255)
                                  aaGCGCTGGCGAAAGATCTCGCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACg                                                             <  2:2863999/100‑1 (MQ=255)
                                  aaGCGCTGGCGAAAGATCACTCCTGGCCGGAGTGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACg                                                             <  2:2062355/100‑1 (MQ=255)
                                    gcgcTGGCGAAAGTTCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGc                                                           <  2:4316921/100‑1 (MQ=255)
                                    gcgcTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGc                                                           <  1:536126/100‑1 (MQ=255)
                                      gcTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGctc                                                         <  1:2698381/100‑1 (MQ=255)
                                      gcTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGctc                                                         <  2:2553086/100‑1 (MQ=255)
                                       cTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGctct                                                        >  2:3217851/1‑100 (MQ=255)
                                        tGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCtctc                                                       <  1:3020518/100‑1 (MQ=255)
                                        tGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCtctc                                                       >  1:2469243/1‑100 (MQ=255)
                                         ggCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCa                                                      <  2:4407653/100‑1 (MQ=255)
                                         ggCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCa                                                      >  2:1975888/1‑100 (MQ=255)
                                          gCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCAc                                                     <  1:2212686/100‑1 (MQ=255)
                                          gCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCCCTCAc                                                     <  1:1906716/100‑1 (MQ=255)
                                           cGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACg                                                    >  1:62435/1‑100 (MQ=255)
                                            gAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGt                                                   <  2:1066196/100‑1 (MQ=255)
                                            gAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGt                                                   >  1:1448785/1‑100 (MQ=255)
                                               aGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCtt                                                <  2:1106521/100‑1 (MQ=255)
                                               aGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCtt                                                <  1:827106/100‑1 (MQ=255)
                                               aGATCTCTCATGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCtt                                                >  2:3915599/1‑100 (MQ=255)
                                                gATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTg                                               <  1:3351133/100‑1 (MQ=255)
                                                  tctctcCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGAc                                             <  1:1812459/100‑1 (MQ=255)
                                                  tctctcCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGAc                                             <  2:4715345/100‑1 (MQ=255)
                                                  tctctcCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGAc                                             >  1:311038/1‑100 (MQ=255)
                                                  tctctcCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGAc                                             >  2:5374169/1‑100 (MQ=255)
                                                   ctctcCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACt                                            <  2:4359444/100‑1 (MQ=255)
                                                   ctctcCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCAGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACt                                            >  2:5000000/1‑100 (MQ=255)
                                                   ctctcCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGGTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACt                                            <  2:1872920/100‑1 (MQ=255)
                                                   ctctcCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGATTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACt                                            >  2:4316615/1‑100 (MQ=255)
                                                   ctctcCTGGCCGGAGCGGCTAGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACt                                            >  1:841745/1‑100 (MQ=255)
                                                     ctcCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTgg                                          <  1:4621772/100‑1 (MQ=255)
                                                     ctcCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTgg                                          >  2:7114/1‑100 (MQ=255)
                                                      tcCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGa                                         >  2:5274703/1‑100 (MQ=255)
                                                        cTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAgc                                       <  2:3137648/100‑1 (MQ=255)
                                                        cTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAgc                                       >  2:4528806/1‑100 (MQ=255)
                                                         tGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAgcg                                      <  1:3569205/100‑1 (MQ=255)
                                                            ctggAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGcgccg                                   <  1:4955444/98‑1 (MQ=255)
                                                            ccGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGcgccg                                   <  1:3242800/100‑1 (MQ=255)
                                                            ccGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGcgccg                                   <  2:4681598/100‑1 (MQ=255)
                                                               gAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCTGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGcac                                <  1:2891515/100‑1 (MQ=255)
                                                                 gCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACaa                              <  2:2833155/100‑1 (MQ=255)
                                                                    gCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAgg                           <  2:1559562/100‑1 (MQ=255)
                                                                    gCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAgg                           <  2:4260616/100‑1 (MQ=255)
                                                                    gCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAgg                           >  1:2606506/1‑100 (MQ=255)
                                                                    gCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAgg                           <  1:3453895/100‑1 (MQ=255)
                                                                    gCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAgg                           <  2:3327282/100‑1 (MQ=255)
                                                                       ggTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACt                        <  2:4184565/100‑1 (MQ=255)
                                                                          gCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCg                     >  2:2595623/1‑100 (MQ=255)
                                                                           cTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACttaga                    >  1:4789581/1‑97 (MQ=255)
                                                                           cTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGc                    <  1:5043817/100‑1 (MQ=255)
                                                                             ggCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTg                  <  2:3289532/100‑1 (MQ=255)
                                                                              gCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTgg                 <  1:1118666/100‑1 (MQ=255)
                                                                               cAAAAAGCTGCGTTTTTTTTTTTTTCTTAATGTCGGTGGATGAAACCGACAAACGGCTCTCCCGTCTTGACTGGAGCGCCGCCCAAAGGCCTTCGCTGGt                >  2:5291200/1‑100 (MQ=255)
                                                                               cAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGt                >  2:3626962/1‑100 (MQ=255)
                                                                               cAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGt                >  2:3017837/1‑100 (MQ=255)
                                                                                aaaaaGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGtt               >  1:1277181/1‑100 (MQ=255)
                                                                                     gCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCcgc          <  1:2510280/100‑1 (MQ=255)
                                                                                      cTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCcgcg         >  2:4972151/1‑100 (MQ=255)
                                                                                      cTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCcgcg         >  2:1430900/1‑100 (MQ=255)
                                                                                        gCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCgat       <  2:1260263/100‑1 (MQ=255)
                                                                                           gTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCgatgat    <  1:5445203/100‑1 (MQ=255)
                                                                                           gTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCgatgat    <  1:4670046/100‑1 (MQ=255)
                                                                                            ttttGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATc   <  1:1602365/100‑1 (MQ=255)
                                                                                                 |                                                                                               
CGCGTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCG  >  NC_000913/3749229‑3749421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A