Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I53 R1	229	158.0	8090706	91.1%	7370633	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,749,326	A→C	D32A (GAT→GCT)	sgbU →	putative L‑xylulose 5‑phosphate 3‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,749,326	0	A	C	99.4%	621.5 / NA	174	D32A (GAT→GCT)	sgbU	putative L‑xylulose 5‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (77/96); total (78/96)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 174 total aligned reads displayed.

CGCGTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCG  >  NC_000913/3749229‑3749421

cgcgTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCtt                                                                                               <  1:527688/100‑1 (MQ=255)

   gtgcgtAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTg                                                                                            >  2:3792585/1‑100 (MQ=255)

   gtgcgtAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTg                                                                                            >  1:3801133/1‑100 (MQ=255)

   gtgcgtAATCATCAGTTAGGGACTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTg                                                                                            <  2:430782/100‑1 (MQ=255)

    tgcgtAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGt                                                                                           <  1:1120376/100‑1 (MQ=255)

    tgcgtAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGt                                                                                           >  1:2977415/1‑100 (MQ=255)

     gcgtAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTc                                                                                          >  1:474970/1‑100 (MQ=255)

      cgtAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCCAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCg                                                                                         <  1:3901134/100‑1 (MQ=255)

         aaTCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGaaa                                                                                      <  2:3465937/100‑1 (MQ=255)

          atcatcAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGaaan                                                                                     <  1:2118899/100‑2 (MQ=255)

            catcaGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGt                                                                                   >  1:560616/1‑100 (MQ=255)

            catcaGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGt                                                                                   >  1:2376863/1‑100 (MQ=255)

             atcaGTTAGGGGGTTATGAAAAAGCGCTGGCGAAAGATCGCTCCGGGCCGGAGCGGCTGGTGCTGGCAAAAGGCTGCGGTTTTGCTTTTGGCGAAATGTc                                                                                  >  2:1756564/1‑100 (MQ=255)

             atcaGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTc                                                                                  >  2:1524752/1‑100 (MQ=255)

             atcaGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTc                                                                                  >  1:25359/1‑100 (MQ=255)

               caGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCgg                                                                                >  2:254014/1‑100 (MQ=255)

                aGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGt                                                                               <  2:2051499/100‑1 (MQ=255)

                aGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGt                                                                               <  1:1332544/100‑1 (MQ=255)

                 gTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTg                                                                              <  1:2839789/100‑1 (MQ=255)

                 gTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTg                                                                              <  1:3777466/100‑1 (MQ=255)

                 gTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTg                                                                              >  1:2457978/1‑100 (MQ=255)

                 gTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGAGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTg                                                                              >  1:3382398/1‑100 (MQ=255)

                   tAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGa                                                                            <  2:2069916/100‑1 (MQ=255)

                    aGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGAt                                                                           <  2:1829602/100‑1 (MQ=255)

                    aGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGAt                                                                           >  2:1532790/1‑100 (MQ=255)

                     gggATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGtttcat                                                                          >  1:2818786/1‑95 (MQ=255)

                     gggATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATg                                                                          <  2:2480026/100‑1 (MQ=255)

                     gggATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATg                                                                          >  1:1403381/1‑100 (MQ=255)

                     gggATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATg                                                                          >  1:81219/1‑100 (MQ=255)

                       gATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGaa                                                                        >  1:1868519/1‑100 (MQ=255)

                        aTTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGaaa                                                                       <  1:1468708/100‑1 (MQ=255)

                         tttATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGAAGCGGCTGGAACAGGAAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAAc                                                                      <  2:1041062/100‑1 (MQ=255)

                         tttATGAAAAAGCGCCGGCGAACGATCTCCCCTGGCCGGAACGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTTGATGAAAc                                                                      <  2:812002/100‑1 (MQ=38)

                          ttATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAAcc                                                                     <  2:2865075/100‑1 (MQ=255)

                           tATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACcg                                                                    >  2:2160796/1‑100 (MQ=255)

                             tGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACcgac                                                                  <  2:568076/100‑1 (MQ=255)

                             tGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGGTTTGCTTTTGTCGAAATGTCGGTGGATGAAACcgac                                                                  >  1:3478770/1‑100 (MQ=255)

                               aaaaaGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACcgacga                                                                <  2:2560837/100‑1 (MQ=255)

                                 aaaGCGCGGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGACAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAAc                                                              <  2:3911320/100‑1 (MQ=255)

                                   aGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACgg                                                            >  1:4033572/1‑100 (MQ=255)

                                      gcTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAACAAGCCGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGctc                                                         >  2:3885498/1‑100 (MQ=39)

                                         ggCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCa                                                      >  2:2991587/1‑100 (MQ=255)

                                         ggCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCCCTCa                                                      >  2:2436545/1‑100 (MQ=255)

                                            gAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGt                                                   >  1:4007652/1‑100 (MQ=255)

                                            gAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGt                                                   >  2:111472/1‑100 (MQ=255)

                                            gAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGt                                                   >  1:733911/1‑100 (MQ=255)

                                              aaGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCt                                                 >  2:2244221/1‑100 (MQ=255)

                                                gATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTg                                               >  2:362786/1‑100 (MQ=255)

                                                gATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTg                                               <  1:1986956/100‑1 (MQ=255)

                                                gATCTCTCCTGGCCGGAGCGGCTGGTGCCGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTg                                               >  2:928062/1‑100 (MQ=255)

                                                 aTCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGa                                              <  2:2643012/100‑1 (MQ=255)

                                                  tctctcCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGAc                                             >  2:1695834/1‑100 (MQ=255)

                                                  tctctcCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGAGGATGAAACCGACGAACGGCTCTCACGTCTTGAc                                             >  1:3055289/1‑100 (MQ=255)

                                                   ctctcCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACATCTTGACt                                            >  2:2662128/1‑100 (MQ=255)

                                                    tctcCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTg                                           <  2:118484/100‑1 (MQ=255)

                                                     ctcCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTgg                                          <  1:3350182/100‑1 (MQ=255)

                                                      tcCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGa                                         <  2:3054877/100‑1 (MQ=255)

                                                       ccTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAg                                        >  2:2989778/1‑100 (MQ=255)

                                                         tGGCCGGAGCGGCTGGTGCTGGCAAAGCGCTGCGGGTTTGCTTTTGGAGAAACGCCGGTGGATGAAACAGACGACCGGCTCTCACGGCTTGACTGGAgcc                                      >  2:3729071/1‑99 (MQ=255)

                                                         tGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAgcg                                      <  2:355648/100‑1 (MQ=255)

                                                            ccGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAGTGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGcgccg                                   <  2:3620573/100‑1 (MQ=255)

                                                            ccGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGcgccg                                   <  2:1278459/100‑1 (MQ=255)

                                                            ccGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGcgccg                                   >  1:3699360/1‑100 (MQ=255)

                                                            ccGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGcgccg                                   <  1:1618831/100‑1 (MQ=255)

                                                            ccGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGcgccg                                   <  1:2045328/100‑1 (MQ=255)

                                                            ccGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCCCTCACGTCTTGACTGGAGcgccg                                   <  2:460299/100‑1 (MQ=255)

                                                             cGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGcgccgc                                  <  2:476663/100‑1 (MQ=255)

                                                               gAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGcac                                <  1:1494243/100‑1 (MQ=255)

                                                               gAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGcac                                <  2:3947532/100‑1 (MQ=255)

                                                                aGCGGCTGGTGCTGGCAAACAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGTATGAAACTGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGcaca                               <  1:3626431/100‑1 (MQ=255)

                                                                   ggCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAg                            <  1:1518097/100‑1 (MQ=255)

                                                                    gCTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAgg                           <  1:3036679/100‑1 (MQ=255)

                                                                     cTGGTGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGa                          >  1:2412727/1‑100 (MQ=255)

                                                                         tGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTc                      <  1:2371713/100‑1 (MQ=255)

                                                                         tGCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTc                      >  1:3559796/1‑100 (MQ=255)

                                                                          gCTGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCg                     >  1:2401051/1‑100 (MQ=255)

                                                                            tGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCt                   >  1:2283883/1‑100 (MQ=255)

                                                                            tGGCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCt                   <  2:1248471/100‑1 (MQ=255)

                                                                             ggCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTg                  >  2:97058/1‑100 (MQ=255)

                                                                             ggCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTg                  <  2:1325515/100‑1 (MQ=255)

                                                                              gCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTgg                 <  1:4084652/100‑1 (MQ=255)

                                                                              gCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTgg                 >  1:578663/1‑100 (MQ=255)

                                                                              gCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTgg                 <  2:1469041/100‑1 (MQ=255)

                                                                              gCAAAAAGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTgg                 <  2:3917269/100‑1 (MQ=255)

                                                                               cAAAAAGCTGCGGTTTTGCTGTTGTCGAAAGGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCGTGACTGGAGCGCCGCACAAAGGACTTCGCTGGt                <  1:1387848/100‑1 (MQ=255)

                                                                                aaaaaGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGtt               <  1:261319/100‑1 (MQ=255)

                                                                                    aGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCcg           >  2:3384415/1‑100 (MQ=255)

                                                                                    aGCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCcg           <  2:322925/100‑1 (MQ=255)

                                                                                     gCTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCcgc          <  1:90225/100‑1 (MQ=255)

                                                                                      cTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCcgcg         >  1:995863/1‑100 (MQ=255)

                                                                                      cTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCcgcg         >  2:2883609/1‑100 (MQ=255)

                                                                                      cTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCcgcg         >  1:1568690/1‑100 (MQ=255)

                                                                                      cTGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCGCTCACGACTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCcgcg         >  2:799220/1‑100 (MQ=255)

                                                                                       tGCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCga        <  2:1111522/100‑1 (MQ=255)

                                                                                        gCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCgat       <  1:68928/100‑1 (MQ=255)

                                                                                        gCGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCgat       <  2:361490/100‑1 (MQ=255)

                                                                                         cGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCgatg      <  2:180200/100‑1 (MQ=255)

                                                                                         cGGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCgatg      <  1:1014723/100‑1 (MQ=255)

                                                                                           gTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCgatgat    <  1:2682909/100‑1 (MQ=255)

                                                                                            ttttGCTTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATc   <  2:2952582/100‑1 (MQ=255)

CGCGTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCG  >  NC_000913/3749229‑3749421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A