Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I51 R1 223 69.9 1534784 95.1% 1459579 214.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,174,612 A→C L262R (CTG→CGG)  parE ← DNA topoisomerase IV, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,174,6120AC100.0% 178.6 / NA 50L262R (CTG→CGG) parEDNA topoisomerase IV, subunit B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (25/25);  total (25/25)

TTCGTCTGCCCGGCAAACTGCGGATCCTGCATTTTTACTGACAGCACATAGGCGCAGCGATCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACAT  >  NC_000913/3174396‑3174840
                                                                                                                                                                                                                         |                                                                                                                                                                                                                                    
ttCGTCTGCCCGGCAAACTGCGGATCCTGCATTTTTACTGACAGCACATAGGCGCAGCGATCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAg                                                                                                                                                                                                                   <  1:435836/236‑1 (MQ=255)
tgtgtgtggccGGCAAACTACGCGTCCTGCATTTTAACCGACGGCCCATAGGCGGAGCGATCCCAGATATCTTCCGCCGACAGCTTTACCCCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTCCC‑GCCCTACATCGTTGGGATAAGGTGGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGc                                                                                                                                                                                                                     <  2:691741/225‑1 (MQ=255)
   gTCTGGCCGGCAAACTGCGGATCCTGCATTTTTACTGACAGCACATAGGCGCAGCGATCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCca                                                                                                                                                                                                                <  1:541658/236‑1 (MQ=255)
       gCCCGGCAAACTGCGGATCCTGCATTTTTACTGACAGCACATAGGCGCAGCGATCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAg                                                                                                                                                                                                             >  2:72151/1‑235 (MQ=255)
       gCCCGGCAAACTGCGGATCCTGCATTTTTACTGACAGCACATAGGCGCAGCGATCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCAACGCCATCCGGCAGCGACAg                                                                                                                                                                                                             >  2:743897/1‑235 (MQ=255)
             cAAACTGCGGATCCTGCATTTTTACTGACAGCACATAGGCGCAGCGATCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGcc                                                                                                                                                                                                      <  1:72151/236‑1 (MQ=255)
                        tCCTGCATTTTTACGGAACGCACATAGGCGCAGCGATCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCca                                                                                                                                                                                               <  2:90793/232‑1 (MQ=255)
                        tCCTGCATTTTGACTGACAGCACATAGGCCCAGCGATCCCAGATATCTTCCGCCGACAGCTTGACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCca                                                                                                                                                                                               <  2:365535/232‑1 (MQ=255)
                            gCATTTTTACTGACAGCCCATAGGCGCAGCGATCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGcc                                                                                                                                                                                                      <  2:394606/221‑1 (MQ=255)
                            gCATTTTTACTGACAGCACATAGGCGCAGCGATCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGcc                                                                                                                                                                                                      >  1:394606/1‑221 (MQ=255)
                                                tAGGCGCAGCGATCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTa                                                                                                                                                                     <  1:743897/234‑1 (MQ=255)
                                                         cGATCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGCTAAACgg                                                                                                                                                          >  2:322538/1‑236 (MQ=255)
                                                             cccAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCca                                                                                                                                                                                                                >  2:641230/1‑178 (MQ=255)
                                                             cccAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCca                                                                                                                                                                                                                <  1:641230/178‑1 (MQ=255)
                                                                      cTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCCTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGc                                                                                                                                             >  2:85444/1‑236 (MQ=255)
                                                                        tCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCg                                                                                                                                            >  2:600137/1‑235 (MQ=255)
                                                                                             cgcgGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGGATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCtt                                                                                                                       >  1:677512/1‑235 (MQ=255)
                                                                                                   aGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGc                                                                                                                                                                                                                     >  2:388490/1‑135 (MQ=255)
                                                                                                   aGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGc                                                                                                                                                                                                                     <  1:388490/135‑1 (MQ=255)
                                                                                                       tatTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTaa                                                                                                            >  1:326521/1‑236 (MQ=255)
                                                                                                         ttGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTaa                                                                                                            <  2:217175/234‑1 (MQ=255)
                                                                                                                             aCGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTa                                                                                                             >  1:712521/1‑213 (MQ=255)
                                                                                                                             aCGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTa                                                                                                             <  2:712521/213‑1 (MQ=255)
                                                                                                                                  tCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCAcc                                                                                   >  2:38819/1‑234 (MQ=255)
                                                                                                                                  tCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAg                                                                                 >  1:755687/1‑236 (MQ=255)
                                                                                                                                    gcgTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCg                                                                               <  2:326521/236‑1 (MQ=255)
                                                                                                                                     cgTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGGTTTTTCGGCAGCGTCGTCAGACCCTTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAACgg                                                                              >  2:426543/1‑235 (MQ=255)
                                                                                                                                            ccagCCCTGGCGCAGTCCATTAACCTGGGTACC‑GCCCTGCATCCTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCg                                                                        <  2:755687/232‑1 (MQ=255)
                                                                                                                                            cAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCgg                                                                       <  1:38819/236‑1 (MQ=255)
                                                                                                                                                       accctccATTGACCTGGGTGCCGGCCTTGCATCGTTGGGAGAAGGTGACCGTACCTTTCGGTCAGCCGTTCCCCGCCTGCCGGCAGCCACAGGAGCCCCCAGTCCACAGCTTCAGTATCACCCGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGAt                                                             <  2:724894/231‑1 (MQ=255)
                                                                                                                                                         aGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGAtctc                                                          <  1:215109/236‑1 (MQ=255)
                                                                                                                                                          gACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCa                                                         >  1:685521/1‑236 (MQ=255)
                                                                                                                                                                tAACATCGGTCAC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTa                                                   <  2:403770/236‑1 (MQ=255)
                                                                                                                                                                       ggTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGATGATCTCATCTTTAAAAGTg                                             >  1:294577/1‑235 (MQ=255)
                                                                                                                                                                               cccTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAAc                                     >  1:340798/1‑236 (MQ=255)
                                                                                                                                                                                  tGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGcc                                  >  2:224515/1‑236 (MQ=255)
                                                                                                                                                                                   gCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCa                                 <  2:340798/236‑1 (MQ=255)
                                                                                                                                                                                   gCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCa                                 <  1:600137/236‑1 (MQ=255)
                                                                                                                                                                                    cATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGa                                                                                                   <  2:393882/169‑1 (MQ=255)
                                                                                                                                                                                    cATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGa                                                                                                   <  2:326687/169‑1 (MQ=255)
                                                                                                                                                                                    cATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGa                                                                                                   >  1:393882/1‑169 (MQ=255)
                                                                                                                                                                                    cATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGa                                                                                                   >  1:326687/1‑169 (MQ=255)
                                                                                                                                                                                      accttGGGATAAGGTTGACGTTGCTTTCGGTCAGCCGTTCCCCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAggg                              <  1:350302/233‑1 (MQ=255)
                                                                                                                                                                                                 aGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAgc                                                                                                                                                                                                         >  1:750599/1‑54 (MQ=255)
                                                                                                                                                                                                 aGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAgc                                                                                                                                                                                                         <  2:750599/54‑1 (MQ=255)
                                                                                                                                                                                                   gttgcagTAGCTCTCCGTCAGCCGTTCACCGTCTTCCGGCAGCCACAGTAGCGCCCGGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAATCGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCtt                 <  2:294577/230‑1 (MQ=255)
                                                                                                                                                                                                       aCGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTGGc             >  1:706575/1‑236 (MQ=255)
                                                                                                                                                                                                        cGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGCTAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAAAGCCATGGCACATTACCGCTTTGGCt            >  1:317372/1‑236 (MQ=255)
                                                                                                                                                                                                             cTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACTAGCGTTGTTCGGGATTGTTGATCTCATCCTTAAAACTGATTTCTACTCCAGGGCACATTACCGTTTTGGCTTTCat       >  2:622732/1‑235 (MQ=255)
                                                                                                                                                                                                                  ggTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACAt  >  1:65499/1‑236 (MQ=255)
                                                                                                                                                                                                                         |                                                                                                                                                                                                                                    
TTCGTCTGCCCGGCAAACTGCGGATCCTGCATTTTTACTGACAGCACATAGGCGCAGCGATCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACC‑GCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACAT  >  NC_000913/3174396‑3174840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A