Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I51 R1	223	69.9	1534784	95.1%	1459579	214.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,710,019	T→G	T153P (ACC→CCC)	eptB ←	KDO phosphoethanolamine transferase, Ca(2+)‑inducible

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,710,019	0	T	G	100.0%	97.2 / NA	29	T153P (ACC→CCC)	eptB	KDO phosphoethanolamine transferase, Ca(2+)‑inducible
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (29/0); total (29/0)

TGAATTATTATCGGAAGATTCATCGACCCGCGCCCAGGCATACAGCCCCAACGCAGAAAGCCAGTTTGATGGCAGATAAGAGTTCGCTACGACACCGCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGTTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCTTTTGACAGGTCGATATCGGTGGTCATGACGGAAGCGATGATGCCATAACCAATGACCACATTAAGGAAGGTCATGTAATAGCTGGCACCTGCGGAAAAGAGCACCACCAGCGATGCCAG  >  NC_000913/3709789‑3710247

tGAATTATTATCGGAAGATTCATCGACCCGCGCCCAGGCATACAGCCCCAACGCAGAAAGCCAGTTTGATGGCAGATAAGAGTTCGCTACGACACCGCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGTGGGTCGCa                                                                                                                                                                                                                                 >  2:462806/1‑236 (MQ=255)

tGAATTATTATCGGAAGATTCATCGACCCGCGCCCAGGCATACAGCCCCAACGCAGAAAGCCAGTTTGATGGCAGATAAGAGTTCGCTACGACACCGCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCa                                                                                                                                                                                                                                 >  1:570743/1‑236 (MQ=255)

 gAATTATTATCGGAAGATTCATCGACCCGCGCCCAGGCATACAGCCCCAACGCAGAAAGCCAGTTTGATGGCAGATAAGAGTTCGCTACGACACCGCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTc                                                                                                                                                                                                                                    >  1:147711/1‑232 (MQ=255)

            ggAAGATTCATCGACCCGCGCCCAGGCATACAGCCCCAACGCAGAAAGCCAGTTTGATGGCAGATAAGAGTTCGCTACGACACCGCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTCGGGGTCGCCGTTGTTGGAGCa                                                                                                                                                                                                                     >  1:356443/1‑236 (MQ=255)

                                               ccAACGCAGAAAGCCAGTTTGATGGCAGATAAGAGTTCGCTACGACACCGCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTCATTCCAgataaggata                                                                                                                                                                                  >  1:636646/1‑236 (MQ=255)

                                               ccAACGCAGAAAGCCAGTTTGATGGCAGATAAGAGTTCGCTACGACACCGCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTCGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTGCTTCCAgataaggata                                                                                                                                                                                  >  2:392592/1‑236 (MQ=255)

                                                         aaGCCAGTTTGATGGCAGATAAGAGTTCGCTACGACACCGCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCaa                                                                                                                                                                          >  1:486491/1‑234 (MQ=255)

                                                                      ggCAGATAAGAGTTCGCTACGACACCGCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGc                                                                                                                                                                                                      >  1:469066/1‑193 (MQ=255)

                                                                                     gCTACGACACCGCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATaa                                                                                                                                             >  1:351353/1‑235 (MQ=255)

                                                                                      cTACGACACCGCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATCAAg                                                                                                                                           >  1:368209/1‑236 (MQ=255)

                                                                                                gCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGGGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATACAGTTCAGACca                                                                                                                                  >  2:706298/1‑235 (MQ=255)

                                                                                                           gCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGCCAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCtttt                                                                                                                      >  2:583298/1‑236 (MQ=255)

                                                                                                           gCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACGGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCtttt                                                                                                                      >  1:728613/1‑236 (MQ=255)

                                                                                                           gCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCtttt                                                                                                                      >  1:635560/1‑236 (MQ=255)

                                                                                                                         gTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCTTTTGACAGGTCGATATc                                                                                                        >  2:136473/1‑236 (MQ=255)

                                                                                                                                            tctGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAAACACTTCTTTTGACAGGTCGATATCGGTGGTCATGACGGAAGCg                                                                                     >  1:665830/1‑236 (MQ=255)

                                                                                                                                                        ccAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCCGATAAGGATAAGNNNCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCTGTTGGCAGGTCGATAACGGTGGGCATGACGGAAGCACTGATACCAtca                                                                         >  2:692179/1‑234 (MQ=255)

                                                                                                                                                                                         ccGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCTTTTGACAGGTCGATATCGGTGGTCATGACGGAAGCgatg                                                                                  >  1:159324/1‑194 (MQ=255)

                                                                                                                                                                                         ccGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCTTTTGACAGGTCGATATCGGTGGCCATGACGGCAGCGATAATGCCATAACCCATGACCACCTTCAGGAAGGGCATGTatatg                                        >  2:120814/1‑232 (MQ=255)

                                                                                                                                                                                           gCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCTTTTGACAGGTCGATATCGGTGGTCATGACGGAAGCGATGATGCCATAACCAATGACCACATTAAGGAAGGTCATGTAATAGCt                                      >  1:643217/1‑236 (MQ=255)

                                                                                                                                                                                                tACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCTTTTGACAGGTCGATATCGGTGGTCATGACGGAAGCGATGATGCCATAACCAATGACCACATTAAGGAAGGTCATGTAATAGCTg                                     >  1:374674/1‑232 (MQ=255)

                                                                                                                                                                                                tACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCTTTTGACAGGTCGATATCGGTGGGCATGACTGAAGCGAGAATGCCATAACCAATGACACCCTCAAGGAAGGGCATGTGATAACTgcgca                                 >  2:426749/1‑232 (MQ=255)

                                                                                                                                                                                                     cgacCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCaga                                                                                                                                                   >  2:228896/1‑117 (MQ=255)

                                                                                                                                                                                                     cgacCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCTTTTGACAGGTCGATATCGGTGGTCATGACGGAAGCGATGATGCCATAACCAATGACCACATTAAGGAAGGTCATGTAATAGCTGGCACCTGCg                            >  2:178151/1‑236 (MQ=255)

                                                                                                                                                                                                     cgacCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCTTTTGACAGGTCGATATCGGTGGTCATGACGGAAGCGATGATGCCATAACCAATGACCACATTAAGGAAGGTCATGTAATAGCCGGCaacgtct                            >  2:390324/1‑230 (MQ=255)

                                                                                                                                                                                                       acCGCCAGGCTGCGAATACGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCTTTTGACAGGTCGATATCGGTGGTCATGACGGAAGCGAGGATGCCATAACCAATGACCACATTAAGGAAGGGCATGTACTACCTGGGACCTGCGGa                          >  2:505410/1‑236 (MQ=255)

                                                                                                                                                                                                                         cGCTGCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCTTTTGACAGGTCGATATCGGTGGTCATGACGGAAGCGATGATGCCATAACCAATGACCACATTAAGGAAGGTCATGTAATAGCTGGCACCTGCGGAAAAGAGCACCACCAGCGa        >  1:204274/1‑236 (MQ=255)

                                                                                                                                                                                                                             gCCCTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACcaac                                                                                                                                >  2:296644/1‑112 (MQ=255)

                                                                                                                                                                                                                               ccTGGGGGTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCTTTTGACAGGTCGATATCGGTGGTCATGACGGAAGCGATGATGCCATAACCAATGACCACATTAAGGAAGGTCATGTAATAGCTGGCACCTGCGGAAAAGAGCACCACCAGCGATGCCAg  >  2:758112/1‑236 (MQ=255)

TGAATTATTATCGGAAGATTCATCGACCCGCGCCCAGGCATACAGCCCCAACGCAGAAAGCCAGTTTGATGGCAGATAAGAGTTCGCTACGACACCGCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGTTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTATTCCAGATAAGGATAAGAGGCAATGCACTAACGGCGATTAACCAGAGAATAAAGTTCAGACCAACAACTTCTTTTGACAGGTCGATATCGGTGGTCATGACGGAAGCGATGATGCCATAACCAATGACCACATTAAGGAAGGTCATGTAATAGCTGGCACCTGCGGAAAAGAGCACCACCAGCGATGCCAG  >  NC_000913/3709789‑3710247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A