Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I51 R1	223	69.9	1534784	95.1%	1459579	214.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,475,797	A→C	I121L (ATT→CTT)	yjgL →	SopA‑central‑domain‑like hexapeptide repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,475,797	0	A	C	100.0%	100.3 / NA	28	I121L (ATT→CTT)	yjgL	SopA‑central‑domain‑like hexapeptide repeat protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (13/15); total (13/15)

AGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATC  >  NC_000913/4475575‑4476004

aGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCAc                                                                                                                                                                                                    <  2:79349/236‑1 (MQ=255)

 ggAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACt                                                                                                                                                                                                   >  2:175235/1‑236 (MQ=255)

   aaTATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTAt                                                                                                                                                                                                 <  1:76527/236‑1 (MQ=255)

                  ggAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGAc                                                                                                                                                                                  >  1:639599/1‑236 (MQ=255)

                       aTTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGt                                                                                                                                                                              <  2:639599/235‑1 (MQ=255)

                          tttAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAg                                                                                                                                                                          >  2:395927/1‑236 (MQ=255)

                                                   aCCCTGGGTTATGTGGATTTTAGCAAACGCTCAAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCcaca                                                                                                                                                 <  1:295758/236‑1 (MQ=255)

                                                              tgtgGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTcc                                                                                                                                                                                                      <  1:249533/172‑1 (MQ=255)

                                                              tgtgGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTcc                                                                                                                                                                                                      >  2:249533/1‑172 (MQ=255)

                                                              tgtgGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCact                                                                                                                                                                                             <  1:241843/181‑1 (MQ=255)

                                                              tgtgGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCact                                                                                                                                                                                             >  2:241843/1‑181 (MQ=255)

                                                                                                                          cTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGa                                                                          >  1:3872/1‑236 (MQ=255)

                                                                                                                             aTCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTa                                                                       >  1:520899/1‑236 (MQ=255)

                                                                                                                                 tATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTaa                                                                   <  2:520899/236‑1 (MQ=255)

                                                                                                                                          gATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCt                                                          <  1:175235/236‑1 (MQ=255)

                                                                                                                                              cTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCg                                                      <  2:3872/236‑1 (MQ=255)

                                                                                                                                                                             gAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATg                                                                                                                       >  1:629964/1‑140 (MQ=255)

                                                                                                                                                                             gAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATg                                                                                                                       <  2:629964/140‑1 (MQ=255)

                                                                                                                                                                               ataatCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGtt                                                            <  1:338571/197‑1 (MQ=255)

                                                                                                                                                                               ataatCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGtt                                                            >  2:338571/1‑197 (MQ=255)

                                                                                                                                                                                        tAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCt                                                                                                                                             >  2:545528/1‑107 (MQ=255)

                                                                                                                                                                                        tAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCt                                                                                                                                             <  1:545528/107‑1 (MQ=255)

                                                                                                                                                                                                           aCCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAg                       >  2:636318/1‑206 (MQ=255)

                                                                                                                                                                                                           aCCCAAGGTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAg                       <  1:636318/206‑1 (MQ=255)

                                                                                                                                                                                                                 ggTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCgtg                                                    >  2:620350/1‑171 (MQ=255)

                                                                                                                                                                                                                 ggTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCgtg                                                    <  1:620350/171‑1 (MQ=255)

                                                                                                                                                                                                                 ggTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATc  <  1:408839/221‑1 (MQ=255)

                                                                                                                                                                                                                 ggTGATTATAGAACTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATc  >  2:408839/1‑221 (MQ=255)

AGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATC  >  NC_000913/4475575‑4476004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A