Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F433 I3 R1	16	161.1	8199666	93.6%	7674887	99.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,006,859	A→C	S200R (AGC→CGC)	ygeW →	putative carbamoyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,006,859	0	A	C	98.0%	537.1 / 0.5	153	S200R (AGC→CGC)	ygeW	putative carbamoyltransferase
Reads supporting (aligned to +/- strand): ref base A (1/2); new base C (74/76); total (75/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.86e-01

Only 100 of 154 total aligned reads displayed.

CAGTCAATGGCTGACCTCGCGTGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCAAGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCT  >  NC_000913/3006763‑3006956

cAGTCAATGGCTGACCTCGCGTGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGc                                                                                                 <  1:3113450/99‑1 (MQ=255)

  gTCAATGGCTGACCTCGCGTGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTa                                                                                               <  1:567688/99‑1 (MQ=255)

  gTCAATGGCTGACCTCGCGTGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTa                                                                                               >  2:1582846/1‑99 (MQ=255)

   tCAATGGCTGACCTCGCGTGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTAt                                                                                              >  1:3611046/1‑99 (MQ=255)

   tCAATGGCTGACCTCGCGTGGGGACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCGCGCTAt                                                                                              <  2:3915278/99‑1 (MQ=255)

     aaTGGCTGACCTCGCGTGGTTACGTGAACACTTTGGTTCAGTGGAAAACCTGAAAGGTAAAAAAATCGCCATGGCCTGGGCCTACTCTCCACGCTATgg                                                                                            <  1:808495/99‑1 (MQ=255)

     aaTGGCTGACCTCGCGTGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATgg                                                                                            <  2:1941666/99‑1 (MQ=255)

     aaTGGCTGACCTCGCGTGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATgg                                                                                            <  1:879590/99‑1 (MQ=255)

     aaTGGCTGACCTCGCGTGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATgg                                                                                            >  1:1937797/1‑99 (MQ=255)

     aaTGGCTGACCTCGCGTGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATgg                                                                                            <  1:1984840/99‑1 (MQ=255)

      aTGGCTGACCTCGCGTGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGc                                                                                           >  1:274995/1‑99 (MQ=255)

         gCTGACCTCGCGTGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCaaa                                                                                        <  1:3677004/99‑1 (MQ=255)

         ccTGACCTAGCGTTGTTACGTGAACACTTTGTTTGACTGGAAGGCCTGAAAGGTAAAAAGATCGCCATGACCTGGGCGTACTCTCCACGCTATGGCaaa                                                                                        <  2:3054388/98‑1 (MQ=255)

          cTGACCTCGCGTGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAAc                                                                                       <  2:1607875/99‑1 (MQ=255)

          cTGACCTCGCGTGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAAc                                                                                       >  2:3604905/1‑99 (MQ=255)

           tGACCTCGCGTGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAAcc                                                                                      <  2:3367280/99‑1 (MQ=255)

            gACCTCGCGTGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCg                                                                                     >  2:3077995/1‑99 (MQ=255)

             aCCTCGCGTGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGc                                                                                    >  1:2800904/1‑99 (MQ=255)

             aCCTCGCGTGGTTACGTGAACACTTTGGTTCACAGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGc                                                                                    >  2:3571522/1‑99 (MQ=255)

                tCGCGTGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGctct                                                                                 >  1:1848802/1‑99 (MQ=255)

                tCGCGTGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGctct                                                                                 >  2:3933573/1‑99 (MQ=255)

                tCGCGTGGTTACATGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGctct                                                                                 >  1:1294010/1‑99 (MQ=255)

                 cgcgTGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGctctc                                                                                <  1:2652045/99‑1 (MQ=255)

                 cgcgTGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGctctc                                                                                <  1:1687309/99‑1 (MQ=255)

                  gcgTGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGctctct                                                                               <  1:2893903/99‑1 (MQ=255)

                  gcgTGGTTACGTGAACACCTAGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGctctct                                                                               >  1:3522827/1‑99 (MQ=255)

                    gTGGTTACGTGAGCACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGt                                                                             <  1:3835130/99‑1 (MQ=255)

                     tGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTa                                                                            <  1:1409710/99‑1 (MQ=255)

                     tGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTa                                                                            >  2:3685486/1‑99 (MQ=255)

                      ggTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTAc                                                                           <  2:1717131/99‑1 (MQ=255)

                       gTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTAcc                                                                          >  1:1003964/1‑99 (MQ=255)

                        ttACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACca                                                                         >  2:1112312/1‑99 (MQ=255)

                          aCGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACcaca                                                                       >  2:2751780/1‑99 (MQ=255)

                           cGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACaa                                                                      <  2:4058989/99‑1 (MQ=255)

                            gTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAg                                                                     <  1:2325591/99‑1 (MQ=255)

                            gTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAg                                                                     >  1:916963/1‑99 (MQ=255)

                            gTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAg                                                                     >  2:1277468/1‑99 (MQ=255)

                            gTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCAAGCTATGGCAAACCGCTCTCTGTACCACAAg                                                                     <  1:1401368/99‑1 (MQ=255)

                              gAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGc                                                                   <  1:3084020/99‑1 (MQ=255)

                                   cTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGcatcat                                                              <  2:3223461/99‑1 (MQ=255)

                                    tttGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCatcatc                                                             >  1:2965071/1‑99 (MQ=255)

                                    tttGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCatcatc                                                             >  2:4037537/1‑99 (MQ=255)

                                    tttGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCatcatc                                                             >  1:649737/1‑99 (MQ=255)

                                        gTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTc                                                         >  1:2155571/1‑99 (MQ=255)

                                        gTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTc                                                         <  1:116336/99‑1 (MQ=255)

                                           cACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCtga                                                      <  1:2757458/99‑1 (MQ=255)

                                           cACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCtga                                                      <  1:3341734/99‑1 (MQ=255)

                                            aCTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCtgat                                                     >  1:1544155/1‑99 (MQ=255)

                                             cTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCtgatg                                                    >  1:2171663/1‑99 (MQ=255)

                                              tGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCtgatga                                                   >  2:912652/1‑99 (MQ=255)

                                              tGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCtgatga                                                   >  2:3029212/1‑99 (MQ=255)

                                              tGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCtgatga                                                   <  1:3398950/99‑1 (MQ=255)

                                               ggAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGAc                                                  >  2:1552839/1‑99 (MQ=255)

                                                gAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACt                                                 >  2:888278/1‑99 (MQ=255)

                                                  aaaCCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCg                                               >  2:2403362/1‑99 (MQ=255)

                                                    aCCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCt                                             >  2:2562229/1‑99 (MQ=255)

                                                     ccTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCtt                                            >  1:759665/1‑99 (MQ=255)

                                                     ccTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCAAGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCtt                                            >  1:696618/1‑99 (MQ=255)

                                                      cTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTc                                           >  1:3465713/1‑99 (MQ=255)

                                                       tGAAAGGTAAAAAAATCGCCATGACCTGGGGCTACTCTCCACGCTACGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCg                                          >  1:1326396/1‑99 (MQ=255)

                                                       tGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCg                                          >  2:477869/1‑99 (MQ=255)

                                                       tGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCAAGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCg                                          <  1:4021505/99‑1 (MQ=255)

                                                        gAAAGGTAAAAAAATCGCCATGCCCTGGGCTTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCgg                                         <  2:761620/99‑1 (MQ=255)

                                                          aaGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTa                                       <  1:1991342/99‑1 (MQ=255)

                                                           aGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTAt                                      <  1:423049/99‑1 (MQ=255)

                                                            ggTAAAAAACTCGCCATGACCTGCGCCTACTCTCCACGCTATCGCAAACCGCTCGCTGTATCACAAGGCATCATCGGTCTGATGCCTCGCTTCGGTATg                                     <  1:3598776/99‑1 (MQ=255)

                                                            ggTAAAAAAATCGCCATGACCTGGGCCTACGCTCCACGCTATGACAAACCGCTCTCTGTACCAAAAGGCAGAATCGGTCTGATGACTCGCTTCGGTATg                                     <  2:2190771/99‑1 (MQ=255)

                                                              tAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGa                                   >  1:2534954/1‑99 (MQ=255)

                                                              tAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGa                                   >  1:1129743/1‑99 (MQ=255)

                                                              tAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGa                                   <  2:2761310/99‑1 (MQ=255)

                                                              tAAAAAAATCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGa                                   >  2:328134/1‑99 (MQ=255)

                                                                 aaaaaTCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGt                                <  1:3758572/99‑1 (MQ=255)

                                                                 aaaaaTCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGt                                >  1:3435900/1‑99 (MQ=255)

                                                                  aaaaTCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTc                               >  1:2311411/1‑99 (MQ=255)

                                                                   aaaTCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCa                              <  2:140933/99‑1 (MQ=255)

                                                                      tCGCCATGACCTGGTCCTCCTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAATGCATCATCGGTCTGATGTCTCGCTTCGGTATGGATGTCAccc                           <  1:2953894/99‑1 (MQ=255)

                                                                      tCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCAccc                           <  2:1103486/99‑1 (MQ=255)

                                                                      tCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCAccc                           <  1:2279462/99‑1 (MQ=255)

                                                                      tCGCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCAccc                           <  2:1923099/99‑1 (MQ=255)

                                                                        gCCATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTg                         >  1:2115173/1‑99 (MQ=255)

                                                                          cATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGc                       <  2:3404463/99‑1 (MQ=255)

                                                                          cATGACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGc                       <  1:2571751/99‑1 (MQ=255)

                                                                             gACCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCa                    >  2:860411/1‑99 (MQ=255)

                                                                              aCCTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCAt                   >  2:560767/1‑99 (MQ=255)

                                                                               ccTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGGCTCGCTGGGGTATGGGTGTCACCCTGGCCCATc                  <  2:2184224/99‑1 (MQ=255)

                                                                                cTGGGCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATcc                 >  1:1814480/1‑99 (MQ=255)

                                                                                 tGGGCCTCCTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGTCTAGCTTCCGTATGGATGTCACCCTTGCCCATCCg                <  2:1316513/99‑1 (MQ=255)

                                                                                    gCCTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGaa             <  1:1776439/99‑1 (MQ=255)

                                                                                     ccTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAg            >  2:3112734/1‑99 (MQ=255)

                                                                                     ccTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAg            <  1:1614999/99‑1 (MQ=255)

                                                                                     ccTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAg            <  1:846262/99‑1 (MQ=255)

                                                                                      cTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAgg           <  1:452193/99‑1 (MQ=255)

                                                                                      cTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAgg           <  2:2627854/99‑1 (MQ=255)

                                                                                      cTACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAgg           <  2:2325191/99‑1 (MQ=255)

                                                                                       tACTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGc          >  1:1062949/1‑99 (MQ=255)

                                                                                        aCTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCt         >  2:2539639/1‑99 (MQ=255)

                                                                                        aCTCTCCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCt         <  2:968829/99‑1 (MQ=255)

                                                                                          tctcCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTAc       >  1:540683/1‑99 (MQ=255)

                                                                                            tcCACGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGa     >  2:1724080/1‑99 (MQ=255)

                                                                                               aaGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCt  >  2:1520608/1‑99 (MQ=255)

CAGTCAATGGCTGACCTCGCGTGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCAAGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCT  >  NC_000913/3006763‑3006956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A