Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,040,512 A→C G104G (GGT→GGG)  cptA ← toxin of CptAB toxin‑antitoxin pair

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,040,5120AC96.6% 494.0 / 4.4 149G104G (GGT→GGG) cptAtoxin of CptAB toxin‑antitoxin pair
Reads supporting (aligned to +/- strand):  ref base A (2/3);  new base C (75/69);  total (77/72)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

Only 100 of 151 total aligned reads displayed.

TCTTTATCTTTGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTACCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCACTCCTGCCCTTGCCAACGCAAACGCCC  >  NC_000913/3040413‑3040607
                                                                                                   |                                                                                               
     atctttGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGcc                                                                                            <  1:1296549/100‑1 (MQ=255)
      tctttGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCa                                                                                           >  2:3547924/1‑100 (MQ=255)
       ctttGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCAt                                                                                          >  1:701883/1‑100 (MQ=255)
        tttGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATc                                                                                         <  2:2316220/100‑1 (MQ=255)
          tGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAg                                                                                       <  1:3878849/100‑1 (MQ=255)
          tGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAg                                                                                       <  1:3638525/100‑1 (MQ=255)
           gCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTACCGCCATCAGa                                                                                      >  2:218940/1‑100 (MQ=255)
             gTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGaan                                                                                    <  1:3233109/100‑2 (MQ=255)
                 ctTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTaa                                                                                <  1:3072700/100‑1 (MQ=255)
                   tgttgCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAAc                                                                              <  2:2469129/100‑1 (MQ=255)
                      tgCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACgca                                                                           >  1:3315154/1‑100 (MQ=255)
                       gCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACgcag                                                                          <  1:1908415/100‑1 (MQ=255)
                        caacaaAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACgcagc                                                                         >  1:763405/1‑100 (MQ=255)
                         gacaaAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACgcagca                                                                        >  1:115978/2‑100 (MQ=255)
                         aactaAATCCGCCGTAAATCCCGCCACTCAGCTTCGTCCATGCTGTCGGCTCCCAGCCATAAATGTTGCCGTTTCCCGCCATCACAACGAAAACgcagca                                                                        >  2:2402143/1‑100 (MQ=255)
                         aacaaAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACgcagca                                                                        >  1:3974619/1‑100 (MQ=255)
                          acaaAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGcat                                                                       >  1:1754727/1‑100 (MQ=255)
                          acaaAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGcat                                                                       >  2:147358/1‑100 (MQ=255)
                            aaaaTCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGcatca                                                                     >  2:470029/1‑100 (MQ=255)
                            aaaaTCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGcatca                                                                     >  1:2823821/1‑100 (MQ=255)
                             aaaTCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGcatcat                                                                    <  1:3506970/100‑1 (MQ=255)
                             aaaTCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGcatcat                                                                    <  2:2621969/100‑1 (MQ=255)
                              aaTCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATg                                                                   <  1:3728078/100‑1 (MQ=255)
                              aaTCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATg                                                                   <  2:3166598/100‑1 (MQ=255)
                              aaTCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCAATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATg                                                                   <  2:2176827/100‑1 (MQ=255)
                               aTCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGc                                                                  <  1:3401244/100‑1 (MQ=255)
                               aTCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGc                                                                  >  2:3297256/1‑100 (MQ=255)
                                tCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGcc                                                                 >  1:2049007/1‑100 (MQ=255)
                                tCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGcc                                                                 <  1:1769046/100‑1 (MQ=255)
                                  cgccgTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTACCGCCATCAGAACGTAAACGCAGCATCATGCCGc                                                               <  1:2102968/100‑1 (MQ=255)
                                     cgTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGctct                                                            >  1:2142520/1‑100 (MQ=255)
                                     cgTAAATCCCGCCATTCAGCTTCGACCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGGTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGctct                                                            >  2:3801501/1‑100 (MQ=255)
                                     cgTAAAGCCCGCCGTTCGGCTGCGTCCATGCTGCCGGCTGCCCGCCATCACGGTTGCCGTGTCCCGCCATCAGAACGTAAACGCAGGATCACGCCGctct                                                            <  2:2707663/100‑1 (MQ=255)
                                       tAAGTCCCGCCCTGCAGCTTCGTCGTTGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACCTAAACGCAGCATCATGCCGCTCTTa                                                          <  2:3189521/100‑1 (MQ=255)
                                       tAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTa                                                          <  2:1014273/100‑1 (MQ=255)
                                       tAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGATTCCCGCCATCAGAACGTAAACGCAGCACCATGCCGCTCTTa                                                          >  1:2859479/1‑100 (MQ=255)
                                        aaaTCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTaa                                                         <  2:1938183/100‑1 (MQ=255)
                                         aaTCTCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAat                                                        <  1:3658003/100‑1 (MQ=255)
                                         aaTCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAat                                                        <  2:114103/100‑1 (MQ=255)
                                         aaTCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAat                                                        <  2:1918176/100‑1 (MQ=255)
                                         aaTCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAat                                                        <  2:604657/100‑1 (MQ=255)
                                          aTCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAatc                                                       >  2:845886/1‑100 (MQ=255)
                                           tCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAatca                                                      >  2:765292/1‑100 (MQ=255)
                                            cccGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAatcat                                                     >  1:778999/1‑100 (MQ=255)
                                              cGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATcc                                                   >  2:3929995/1‑100 (MQ=255)
                                                ccATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAGCGCAGCATCATGCCGCTCTTAATCATCCAc                                                 <  2:2071463/100‑1 (MQ=255)
                                                 cATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACg                                                >  2:3433962/1‑100 (MQ=255)
                                                  aTTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACgg                                               <  2:1330194/100‑1 (MQ=255)
                                                  aTTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAACCATCCACgg                                               <  1:3979244/100‑1 (MQ=255)
                                                   ttCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGt                                              <  2:3478566/100‑1 (MQ=255)
                                                       gCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCtt                                          >  1:2920414/1‑100 (MQ=255)
                                                       gCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCtt                                          >  1:1643449/1‑100 (MQ=255)
                                                        cTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCttt                                         <  2:3302300/100‑1 (MQ=255)
                                                          tCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTACCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGa                                       <  2:3474822/100‑1 (MQ=255)
                                                           cGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCTGTTTCCCGCCATCAGAACGTTAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGAc                                      <  2:3134457/100‑1 (MQ=255)
                                                           cGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGAc                                      <  2:884516/100‑1 (MQ=255)
                                                           cGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGAc                                      >  2:3879531/1‑100 (MQ=255)
                                                           cGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGAc                                      <  1:679460/100‑1 (MQ=255)
                                                               cATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATg                                  >  2:639351/1‑100 (MQ=255)
                                                               cATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATg                                  <  2:2414790/100‑1 (MQ=255)
                                                                aTGCTGTTGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGc                                 >  2:1420871/1‑100 (MQ=255)
                                                                aTGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGc                                 >  1:369291/1‑100 (MQ=255)
                                                                 tGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCt                                >  2:3742773/1‑100 (MQ=255)
                                                                 tGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCt                                >  1:1511731/1‑100 (MQ=255)
                                                                  gCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCtn                               <  1:3260479/100‑2 (MQ=255)
                                                                  gCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTc                               >  2:2243234/1‑100 (MQ=255)
                                                                     gTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCAc                            <  1:2605032/100‑1 (MQ=255)
                                                                     gTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCAc                            >  1:2555851/1‑100 (MQ=255)
                                                                     gTCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCAc                            >  1:965048/1‑100 (MQ=255)
                                                                      tCGGCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCACt                           <  1:3172269/100‑1 (MQ=255)
                                                                        ggCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCACTcc                         >  1:1001893/1‑100 (MQ=255)
                                                                        ggCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCACTcc                         <  2:1469267/100‑1 (MQ=255)
                                                                        ggCTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCACTcc                         >  1:1001859/1‑100 (MQ=255)
                                                                          cTGCCAGCCATAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCACTCCTg                       <  2:3717946/100‑1 (MQ=255)
                                                                            gccagccaTAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCACTCCTGcc                     >  2:2957627/1‑100 (MQ=255)
                                                                            gccagccaTAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCACTCCTGcc                     <  2:1363113/100‑1 (MQ=255)
                                                                             ccagccaTAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCACTCCTGccc                    <  2:566343/100‑1 (MQ=255)
                                                                             ccagccaTAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCACTCCTGccc                    >  2:2366159/1‑100 (MQ=255)
                                                                             ccagccaTAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCACTCCTGccc                    >  2:2313738/1‑100 (MQ=255)
                                                                              cagccaTAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCACTCCTGCCCt                   >  1:2394134/1‑100 (MQ=255)
                                                                               agccaTAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCACTCCTGCCCtt                  <  1:1531275/100‑1 (MQ=255)
                                                                               agccaTAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCACTCCTGCCCtt                  >  1:3798901/1‑100 (MQ=255)
                                                                                gccaTAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCACTCCTGCCCTTg                 <  2:3438332/100‑1 (MQ=255)
                                                                                gccaTAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCACTCCTGCCCTTg                 >  1:41015/1‑100 (MQ=255)
                                                                                gccaTAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCACTCCTGCCCTTg                 >  2:2084782/1‑100 (MQ=255)
                                                                                 ccaTAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCACTCCTGCCCTTGc                <  2:1847350/100‑1 (MQ=255)
                                                                                  caTAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCACTCCTGCCCTTGcc               >  2:2795967/1‑100 (MQ=255)
                                                                                    tAAATGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGAGCTTTGACGATGCTCCACTCCTGCCCTTGCCaa             >  1:457978/1‑100 (MQ=255)
                                                                                      aaTGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCACTCCTGCCCTTGCCAACg           <  1:244116/100‑1 (MQ=255)
                                                                                       aTGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCACTCCTGCCCTTGCCAACGc          >  1:142114/1‑100 (MQ=255)
                                                                                       aTGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCACTCCTGCCCTTGCCAACGc          <  2:2225854/100‑1 (MQ=255)
                                                                                        tGTTGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCACTCCTGCCCTTGCCAACGCa         >  1:2137222/1‑100 (MQ=255)
                                                                                           tGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCACTCCTGCCCTTGCCAACGCAAAc      <  2:1858529/100‑1 (MQ=255)
                                                                                           tGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCACTCCTGCCCTTGCCAACGCAAAc      >  1:905452/1‑100 (MQ=255)
                                                                                           tGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCACTCCTGCCCTTGCCAACGCAAAc      >  1:875753/1‑100 (MQ=255)
                                                                                           tGCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCACTCCTGCCCTTGCCAACGCAAAc      >  1:874448/1‑100 (MQ=255)
                                                                                            gCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCACTCCTGCCCTTGCCAACGCAAACg     <  2:2572138/100‑1 (MQ=255)
                                                                                            gCCGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCACTCCTGCCCTTGCCAACGCAAACg     >  1:1827055/1‑100 (MQ=255)
                                                                                              cGTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCACTCCTGCCCTTGCCAACGCAAACGcc   <  2:3622881/100‑1 (MQ=255)
                                                                                               gTTTCCCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCACTCCTGCCCTTGCCAACGCAAACGccc  >  2:3930262/1‑100 (MQ=255)
                                                                                                   |                                                                                               
TCTTTATCTTTGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTACCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTAATCATCCACGGTGCTTTGACGATGCTCCACTCCTGCCCTTGCCAACGCAAACGCCC  >  NC_000913/3040413‑3040607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A