Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F17 I0 R2
|
162 |
60.6 |
4616657 |
98.5% |
4547407 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
695,215 |
C→T |
10.9% |
A55T (GCT→ACT) |
miaB ← |
isopentenyl‑adenosine A37 tRNA methylthiolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 695,215 | 0 | C | T | 10.9%
| 134.2
/ 6.6
| 46 | A55T (GCT→ACT) | miaB | isopentenyl‑adenosine A37 tRNA methylthiolase |
| Reads supporting (aligned to +/- strand): ref base C (21/20); new base T (2/3); total (23/23) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 |
ATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGAT > W3110S.gb/695145‑695283
|
aTAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGc > 1:3519191/1‑71 (MQ=255)
aTAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGc > 1:3196733/1‑71 (MQ=255)
tAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGcc < 1:2005041/71‑1 (MQ=255)
cTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTcgcg > 1:1484552/1‑70 (MQ=255)
gATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGTCTTCTCGCGGAtt > 1:2586261/1‑71 (MQ=255)
gATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGAtt < 1:780166/71‑1 (MQ=255)
aTTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGAtt > 1:3204107/1‑70 (MQ=255)
ttcttcTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGAtt > 1:53732/1‑69 (MQ=255)
tcttctCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGc > 1:2445414/1‑55 (MQ=255)
ctctTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGc < 1:3801353/68‑1 (MQ=255)
ctctTTTAACAGTTTCAAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAgg > 1:2918102/1‑71 (MQ=255)
ttttAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTcgcg < 1:418571/57‑1 (MQ=255)
ttAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGa > 1:4404984/1‑57 (MQ=255)
ttAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGa > 1:766588/1‑57 (MQ=255)
tAACAGTTTCCAGCGACCCAACTGATGGAATACTTTTTCCTGAGTCTtc < 1:2949740/49‑1 (MQ=39)
aCAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTcag > 1:4360332/1‑70 (MQ=255)
cAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGTCTTCTCGCGGATTGAGCAGgtg < 1:1586059/64‑1 (MQ=255)
aGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGTCTTCTCGCGGATTGAGCAGGTGTTcagcag < 1:824381/71‑1 (MQ=255)
aGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTcagaag < 1:2962685/71‑1 (MQ=255)
gTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTcagcag > 1:3852402/1‑70 (MQ=255)
tttCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTtctc < 1:1796967/45‑1 (MQ=255)
tttCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTtctc < 1:81443/45‑1 (MQ=255)
ttCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTcg < 1:916213/45‑1 (MQ=255)
gCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAg > 1:1545592/1‑49 (MQ=255)
gCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAg < 1:4003229/52‑1 (MQ=255)
ccAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGct > 1:2736116/1‑71 (MQ=255)
aaCTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGgtg > 1:1016580/1‑48 (MQ=255)
aCTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGcttat > 1:3351497/1‑69 (MQ=255)
cTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGa > 1:2270465/1‑39 (MQ=255)
cTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTcagcag > 1:3205972/1‑54 (MQ=255)
cTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGcttctt < 1:1297944/71‑1 (MQ=255)
aGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACg > 1:4553470/1‑71 (MQ=255)
gACTTTTTCCTGAGTCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGt > 1:1882285/1‑71 (MQ=255)
gACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGt > 1:3415491/1‑71 (MQ=255)
gACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGt < 1:437418/71‑1 (MQ=255)
gACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGt < 1:4099318/71‑1 (MQ=255)
aCTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTcag < 1:134050/41‑1 (MQ=255)
aCTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTc < 1:4427814/71‑1 (MQ=255)
aCTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTc > 1:3330158/1‑71 (MQ=255)
cTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTc < 1:3762728/70‑1 (MQ=255)
tttttCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGc > 1:3123114/1‑54 (MQ=255)
ttttCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGt > 1:3729296/1‑71 (MQ=255)
tttCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTc < 1:2506045/35‑1 (MQ=255)
tttCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTcc > 1:3648395/1‑59 (MQ=255)
tttCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCg > 1:3090435/1‑68 (MQ=255)
tCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCa < 1:154096/70‑1 (MQ=255)
tCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCa < 1:145508/70‑1 (MQ=255)
tGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATcc < 1:3521544/45‑1 (MQ=255)
aGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGa < 1:3909127/70‑1 (MQ=255)
aGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGAt < 1:1280768/71‑1 (MQ=255)
|
ATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGAT > W3110S.gb/695145‑695283
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
