Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F17 I0 R2
|
162 |
60.6 |
4616657 |
98.5% |
4547407 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
861,111 |
G→A |
10.4% |
L640L (CTG→TTG) |
ybiW ← |
predicted pyruvate formate lyase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 861,111 | 0 | G | A | 10.4%
| 235.2
/ 13.8
| 77 | L640L (CTG→TTG) | ybiW | predicted pyruvate formate lyase |
| Reads supporting (aligned to +/- strand): ref base G (24/45); new base A (4/4); total (28/49) |
| Fisher's exact test for biased strand distribution p-value = 4.52e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01 |
GACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACTTC > W3110S.gb/861044‑861181
|
gaCGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtc > 1:3768545/1‑71 (MQ=255)
gaCGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtc > 1:3195879/1‑71 (MQ=255)
aCCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa > 1:390344/1‑71 (MQ=255)
ccGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:3529165/71‑1 (MQ=255)
ccGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:2558341/71‑1 (MQ=255)
cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGatat < 1:3367507/71‑1 (MQ=255)
cgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAg < 1:3002994/71‑1 (MQ=255)
cgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAg > 1:1833014/1‑71 (MQ=255)
tAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGt > 1:3256275/1‑71 (MQ=255)
aaTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTc > 1:504897/1‑71 (MQ=255)
aTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCt < 1:906763/71‑1 (MQ=255)
tAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCt > 1:984924/1‑70 (MQ=255)
tAGTTGACGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTg < 1:2061960/71‑1 (MQ=255)
gTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGAtata > 1:2456791/1‑63 (MQ=255)
gTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGAt < 1:3879246/71‑1 (MQ=255)
ttGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTTGATATTGGTCTGATa < 1:1626353/71‑1 (MQ=255)
ttGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCt < 1:1837963/67‑1 (MQ=255)
ttGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATa < 1:1916990/71‑1 (MQ=255)
ttGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATa < 1:1275690/71‑1 (MQ=255)
ttGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGa < 1:1821212/58‑1 (MQ=255)
ttccgccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATaa < 1:3625976/69‑1 (MQ=255)
gccgccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATaa < 1:3046347/70‑1 (MQ=255)
ccgccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATaa < 1:2431432/69‑1 (MQ=255)
ccAACCGGACCACGACTGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGAt < 1:663312/64‑1 (MQ=255)
cAACGGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGt < 1:1156506/58‑1 (MQ=255)
cAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGcgcg < 1:131758/70‑1 (MQ=255)
cAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGa < 1:3583355/71‑1 (MQ=255)
aaCCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAg > 1:1378833/1‑71 (MQ=255)
aaCCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAg > 1:1193506/1‑71 (MQ=255)
aCCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGc > 1:2242999/1‑71 (MQ=255)
aCCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGc < 1:816599/71‑1 (MQ=255)
aCCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTAGTCGATATAGGTCTGATAAGCGCGAGc > 1:3211280/1‑71 (MQ=255)
cGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:1916978/47‑1 (MQ=255)
cGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGc > 1:1416510/1‑69 (MQ=255)
cGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCca < 1:2601823/71‑1 (MQ=255)
ggACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCcag < 1:2666780/71‑1 (MQ=255)
gACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGt < 1:95220/38‑1 (MQ=255)
gACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCcag > 1:1137198/1‑70 (MQ=255)
aCCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:2882320/44‑1 (MQ=255)
ccACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata > 1:4384140/1‑45 (MQ=255)
ccACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata > 1:1857583/1‑45 (MQ=255)
ccACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCcagcag > 1:1723073/1‑71 (MQ=255)
aCGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCg < 1:4167001/71‑1 (MQ=255)
cGATCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa > 1:2764122/1‑40 (MQ=255)
cGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCATCAGCGt < 1:4187210/71‑1 (MQ=255)
cGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGt < 1:3844294/71‑1 (MQ=255)
cGACAGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGt < 1:315362/71‑1 (MQ=255)
gACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTa > 1:3409938/1‑71 (MQ=255)
aCCGTAGCGCGGATTAT‑GTACTGTTTCAGTTCGTCGATATAGGTCTGATAAgcgct < 1:1708916/56‑2 (MQ=255)
ccGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata > 1:4215939/1‑39 (MQ=255)
ccGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATc < 1:1400007/71‑1 (MQ=255)
gTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGa < 1:3347149/71‑1 (MQ=255)
gcgcGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACa < 1:2042956/70‑1 (MQ=255)
gcgcGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAg > 1:4481664/1‑71 (MQ=255)
gcgcGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACa < 1:2242564/70‑1 (MQ=255)
ggATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTat > 1:381740/1‑70 (MQ=255)
gATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTatca < 1:771253/71‑1 (MQ=255)
aTTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTatcat < 1:3040909/71‑1 (MQ=255)
aTTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTatcat < 1:276061/71‑1 (MQ=255)
ttATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTatcatc > 1:3982577/1‑71 (MQ=255)
ttATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTatcatc < 1:3620446/71‑1 (MQ=255)
tGGTACTGTTTCAGTTCGTCGATATAGGTCTGCTAAGCGCGAGCCAGCAGCGTATCGACAGTATCAtcgtc < 1:1960569/71‑1 (MQ=255)
tGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCAtcgt < 1:447600/70‑1 (MQ=255)
gTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCAtcgtcg < 1:116310/70‑1 (MQ=255)
tACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGa < 1:268216/39‑1 (MQ=255)
tACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTa < 1:161664/60‑1 (MQ=255)
tACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGtt < 1:2774723/71‑1 (MQ=255)
tACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCCTATCGACAGTa < 1:4532778/60‑1 (MQ=255)
tACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGAGAGCcagca < 1:3503057/46‑1 (MQ=255)
cTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGc < 1:4238413/71‑1 (MQ=255)
cTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGc > 1:3597478/1‑71 (MQ=255)
gTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCg > 1:4454455/1‑71 (MQ=255)
tttCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGcc < 1:1339209/69‑1 (MQ=255)
tCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACa > 1:2412600/1‑50 (MQ=255)
tCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTAc > 1:4392299/1‑71 (MQ=255)
tCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTAc > 1:2381007/1‑71 (MQ=255)
aGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACtt < 1:1373143/71‑1 (MQ=255)
gTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACTTc < 1:2921677/71‑1 (MQ=255)
|
GACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACTTC > W3110S.gb/861044‑861181
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
