Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F21 I0 R2
|
316 |
65.6 |
5147759 |
97.4% |
5013917 |
60.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
861,111 |
G→A |
35.1% |
L640L (CTG→TTG) |
ybiW ← |
predicted pyruvate formate lyase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 861,111 | 0 | G | A | 35.1%
| 63.4
/ 54.7
| 57 | L640L (CTG→TTG) | ybiW | predicted pyruvate formate lyase |
| Reads supporting (aligned to +/- strand): ref base G (13/24); new base A (14/6); total (27/30) |
| Fisher's exact test for biased strand distribution p-value = 1.46e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.38e-01 |
TGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTA > W3110S.gb/861043‑861177
|
tgaCGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGtt < 1:2696483/71‑1 (MQ=255)
tgaCGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGtt < 1:1432913/71‑1 (MQ=255)
aCGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcg > 1:4147928/1‑71 (MQ=255)
gTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcgtc > 1:3523417/1‑71 (MQ=255)
tACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcgtcg < 1:1282356/71‑1 (MQ=255)
aCCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcgtcg < 1:2441284/70‑1 (MQ=255)
cccGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:4925954/70‑1 (MQ=255)
cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGatat < 1:2144494/71‑1 (MQ=255)
cgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAg < 1:1374212/71‑1 (MQ=255)
tAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGt < 1:3599830/57‑1 (MQ=255)
tAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:3861435/64‑1 (MQ=255)
tAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGt > 1:2997631/1‑71 (MQ=255)
aTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCt > 1:4951682/1‑71 (MQ=255)
aTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCt > 1:3885783/1‑71 (MQ=255)
aTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCt > 1:2557563/1‑71 (MQ=255)
tAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAg < 1:1831219/66‑1 (MQ=255)
tAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAg < 1:4479774/66‑1 (MQ=255)
gTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGt > 1:4865213/1‑66 (MQ=255)
gTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGa > 1:2580554/1‑70 (MQ=255)
tGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATa < 1:865831/70‑1 (MQ=255)
gccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAgcgc < 1:199855/71‑1 (MQ=255)
aaCCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGa > 1:4862934/1‑70 (MQ=255)
aaCCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAg > 1:1607168/1‑71 (MQ=255)
ccGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCt < 1:4380620/57‑1 (MQ=255)
cGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGAtata > 1:2966023/1‑51 (MQ=255)
cGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGcc < 1:5145339/70‑1 (MQ=255)
cGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATaa > 1:1348817/1‑61 (MQ=255)
cGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATaa > 1:1051583/1‑61 (MQ=255)
ggACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCcag > 1:4038961/1‑71 (MQ=255)
ccACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCcagcag < 1:2592458/71‑1 (MQ=255)
ccACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCcagcag > 1:2838576/1‑71 (MQ=255)
cACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCcagcag < 1:875159/70‑1 (MQ=255)
aCGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAgcgc < 1:306357/59‑1 (MQ=255)
aCGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCg > 1:2031801/1‑71 (MQ=255)
cGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGAtata > 1:2932438/1‑44 (MQ=255)
cGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCg > 1:1969905/1‑70 (MQ=255)
cGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGt < 1:2613734/71‑1 (MQ=255)
cGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAg > 1:1397353/1‑41 (MQ=255)
gTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCATCAGCGTATCGa < 1:3127468/71‑1 (MQ=255)
gTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGa < 1:2414963/71‑1 (MQ=255)
gTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGa < 1:4857184/71‑1 (MQ=255)
cgcgGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGt < 1:3470682/71‑1 (MQ=255)
cgcgGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGt < 1:2809458/71‑1 (MQ=255)
gATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAgcgc < 1:2596604/45‑1 (MQ=255)
gATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAgcgc < 1:2280321/45‑1 (MQ=255)
gATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAgcgc < 1:4970165/45‑1 (MQ=255)
aTTATGGTACTGTTTCAGTTCGTCGATATAGGTCt < 1:214345/35‑1 (MQ=255)
aTTATGGTACTGTTTCAGTTCGTCGATATAGGTCt < 1:1811852/35‑1 (MQ=255)
tATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCAtcg > 1:3356060/1‑71 (MQ=255)
tGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCAtcgtc < 1:645603/71‑1 (MQ=255)
gTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATcgtcgt > 1:4561837/1‑71 (MQ=255)
gTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATcgtcgt > 1:3678870/1‑71 (MQ=255)
gTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGAGAGCCAGCAGCGTATCGACAGTATCATcgtcgt > 1:1457864/1‑71 (MQ=255)
aCTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTg > 1:4255439/1‑71 (MQ=255)
gTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCg > 1:4331342/1‑71 (MQ=255)
gTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCg > 1:2636280/1‑71 (MQ=255)
ttCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTa > 1:2102297/1‑71 (MQ=255)
|
TGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTA > W3110S.gb/861043‑861177
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
