Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
861,111 |
G→A |
L640L (CTG→TTG) |
ybiW ← |
predicted pyruvate formate lyase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 861,111 | 0 | G | A | 100.0%
| 145.7
/ NA
| 44 | L640L (CTG→TTG) | ybiW | predicted pyruvate formate lyase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/24); total (20/24) |
CGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCG > W3110S.gb/861052‑861149
|
cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAAtt < 1:3957189/62‑1 (MQ=255)
cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAAtt < 1:80179/62‑1 (MQ=255)
cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAAtt < 1:2303406/62‑1 (MQ=255)
cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAAtt < 1:674902/62‑1 (MQ=255)
gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATtc < 1:852049/62‑1 (MQ=255)
gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATtc < 1:3756777/62‑1 (MQ=255)
gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATtc < 1:2335802/62‑1 (MQ=255)
gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATtc < 1:4322763/62‑1 (MQ=255)
gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATtc < 1:475099/62‑1 (MQ=255)
gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATtc < 1:2753878/62‑1 (MQ=255)
gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATtc < 1:359441/62‑1 (MQ=255)
gagTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATtc < 1:2535583/60‑1 (MQ=255)
tGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAg > 1:282178/1‑62 (MQ=255)
gccgccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATtc < 1:1565741/51‑1 (MQ=255)
gccgccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATtc < 1:412324/51‑1 (MQ=255)
ccgccAACCGGTCCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGt > 1:1288680/1‑62 (MQ=255)
ccgccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGt > 1:4560966/1‑62 (MQ=255)
ccgccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGt > 1:4554217/1‑62 (MQ=255)
ccgccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGt > 1:3565820/1‑62 (MQ=255)
ccgccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGt > 1:2444616/1‑62 (MQ=255)
ccgccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGt > 1:3723058/1‑62 (MQ=255)
ccgccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGt > 1:4264603/1‑62 (MQ=255)
ccgccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGt > 1:4026921/1‑62 (MQ=255)
ccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGt > 1:2939279/1‑59 (MQ=255)
ccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCt > 1:1928688/1‑61 (MQ=255)
ccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGACt > 1:1861562/1‑61 (MQ=255)
aaCCGGAGCACGACCGTAGCGCGGATTATGGTACTGTTTCAATtcgtc > 1:994379/1‑48 (MQ=38)
aaCCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATtcgtc > 1:3933102/1‑48 (MQ=255)
aaCCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATtcgtc > 1:762320/1‑48 (MQ=255)
ccACGACCGTAGCGCGGATTATGGTACTGTTTCAATtc < 1:4603427/38‑1 (MQ=255)
aCGACCGTAGCGCGGATTATGGTACTGTTTCAATtc < 1:1568834/36‑1 (MQ=255)
cGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGa > 1:4283294/1‑60 (MQ=255)
cGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGa > 1:361406/1‑60 (MQ=255)
cGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGa > 1:2957753/1‑60 (MQ=255)
cGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGa > 1:2683219/1‑60 (MQ=255)
cGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGa > 1:569464/1‑60 (MQ=255)
gcgcGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCg < 1:1473909/62‑1 (MQ=255)
gcgcGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCg < 1:4104952/62‑1 (MQ=255)
gcgcGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCg < 1:2213167/62‑1 (MQ=255)
gcgcGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCg < 1:2596491/62‑1 (MQ=255)
gcgcGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCg < 1:4594265/62‑1 (MQ=255)
gcgcGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCg < 1:3245686/62‑1 (MQ=255)
gcgcGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCg < 1:4505267/62‑1 (MQ=255)
gcgcGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCg < 1:3884201/62‑1 (MQ=255)
|
CGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCG > W3110S.gb/861052‑861149
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
