Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F17 I0 R2
|
162 |
60.6 |
4616657 |
98.5% |
4547407 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,472,611 |
G→A |
15.5% |
G144G (GGC→GGT) |
btuB ← |
vitamin B12/cobalamin outer membrane transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,472,611 | 0 | G | A | 15.5%
| 113.7
/ 12.7
| 45 | G144G (GGC→GGT) | btuB | vitamin B12/cobalamin outer membrane transporter |
| Reads supporting (aligned to +/- strand): ref base G (17/21); new base A (3/4); total (20/25) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01 |
TTGCTTCCCCACCCTGCTGAAATTTCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAAGCGCAA > W3110S.gb/3472543‑3472681
|
ttGCTTCCCCACCCTGCTGAAATTTCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGcc < 1:3802195/71‑1 (MQ=255)
tgctgAAATTTCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAAc > 1:2754331/1‑71 (MQ=255)
gAAATTTCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCAt > 1:3576507/1‑70 (MQ=255)
gAAATTTCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCAt > 1:175722/1‑70 (MQ=255)
aaaTTTCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCACCTATTGCATCGGAACCATa < 1:3161077/70‑1 (MQ=255)
tttCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACa < 1:1877741/71‑1 (MQ=255)
tttCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACa < 1:740064/71‑1 (MQ=255)
ccgttccgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAg < 1:4267718/69‑1 (MQ=255)
cgttccgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCAt > 1:1868634/1‑53 (MQ=255)
cgttccgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCg < 1:3682337/70‑1 (MQ=255)
ttccgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCg < 1:333277/68‑1 (MQ=255)
ttccgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAg < 1:1928278/71‑1 (MQ=255)
tccgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTa < 1:2036961/44‑1 (MQ=255)
tccgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTAt > 1:1165898/1‑45 (MQ=255)
ccgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATa < 1:2922426/59‑1 (MQ=255)
ccgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAgcg > 1:4472316/1‑71 (MQ=255)
cgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAgcg < 1:4384579/70‑1 (MQ=255)
cgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAgcgc < 1:2813526/71‑1 (MQ=255)
gggTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATaa > 1:2049283/1‑58 (MQ=255)
gggCTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAGcgcg < 1:3695200/71‑1 (MQ=255)
ttCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAGcgcg < 1:2332830/68‑1 (MQ=255)
tCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGcc > 1:155958/1‑70 (MQ=255)
cgcgTCGTGATGATATTCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGat < 1:2438413/71‑1 (MQ=255)
gcgTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGata > 1:3317586/1‑71 (MQ=255)
cgtcgtGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAgc > 1:387711/1‑56 (MQ=255)
gtcgtGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGatat < 1:2261983/70‑1 (MQ=255)
cgtGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAg < 1:972212/52‑1 (MQ=255)
cgtGATGATATTCAACACCCCGCCTATTGCATCGGAACCATAAACAGCg > 1:911255/1‑49 (MQ=255)
gatgatATTCACCACCCCGCCTATTGCATCGGAACCATaaa > 1:347252/1‑41 (MQ=255)
atgatATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAg > 1:1750751/1‑48 (MQ=255)
atgatATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCaa > 1:4346298/1‑70 (MQ=255)
atgatATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCaa > 1:3244239/1‑70 (MQ=255)
tgatATTCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAaca > 1:3475371/1‑71 (MQ=255)
atatTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAgcg < 1:2603604/47‑1 (MQ=255)
atatTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAacac < 1:3764373/70‑1 (MQ=255)
tatTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGc < 1:195642/71‑1 (MQ=255)
tatTCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGc > 1:3091842/1‑71 (MQ=255)
atTCACCACCCCGCCTATTGCATCGGAACCATAAACAGc > 1:4550399/1‑39 (MQ=255)
ttCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTg < 1:2869519/71‑1 (MQ=255)
accaccCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGAc < 1:2095846/71‑1 (MQ=255)
accaccCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGAc < 1:609372/71‑1 (MQ=255)
caccCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACaa < 1:2915821/71‑1 (MQ=255)
caccCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACaa < 1:4239146/71‑1 (MQ=255)
ccGCATATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAAgcg > 1:2931004/1‑70 (MQ=255)
cGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGa > 1:4139522/1‑63 (MQ=255)
gCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAAGCGCaa > 1:4097727/1‑71 (MQ=255)
|
TTGCTTCCCCACCCTGCTGAAATTTCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAAGCGCAA > W3110S.gb/3472543‑3472681
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
