Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F17 I0 R2
|
162 |
60.6 |
4616657 |
98.5% |
4547407 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,392,090 |
G→T |
20.8% |
L655M (CTG→ATG) |
yjeP ← |
predicted mechanosensitive channel |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,392,090 | 0 | G | T | 20.8%
| 137.2
/ 16.5
| 48 | L655M (CTG→ATG) | yjeP | predicted mechanosensitive channel |
| Reads supporting (aligned to +/- strand): ref base G (14/24); new base T (6/4); total (20/28) |
| Fisher's exact test for biased strand distribution p-value = 2.82e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGCCAATCATCATGTTCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCA > W3110S.gb/4392021‑4392156
|
cgcCAATCATCATGTTCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGc < 1:730761/71‑1 (MQ=255)
aaTCATCATGTTCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGaa > 1:2007172/1‑71 (MQ=255)
atcatGTTCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATccc > 1:1964165/1‑71 (MQ=255)
atcatGTTCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATccc > 1:1821039/1‑71 (MQ=255)
tcatGTTCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCg > 1:2080798/1‑71 (MQ=255)
atGTTCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAg > 1:1003185/1‑59 (MQ=255)
tGTTCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGcc < 1:1203841/71‑1 (MQ=255)
ttCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCtt > 1:1146950/1‑71 (MQ=255)
ttCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCt < 1:1239297/70‑1 (MQ=255)
ccACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCtttt > 1:546557/1‑71 (MQ=255)
ccACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCttt < 1:1559972/70‑1 (MQ=255)
ccACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCttt < 1:2528720/70‑1 (MQ=255)
cacaGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCttttt < 1:811965/71‑1 (MQ=255)
cacaGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCttttt < 1:2337439/71‑1 (MQ=255)
cacaGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCttttt < 1:1296217/71‑1 (MQ=255)
acaGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATc > 1:3822284/1‑59 (MQ=255)
atatGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCt < 1:1325152/71‑1 (MQ=255)
tggttggtAATGTTTTCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGCTGa < 1:494221/70‑1 (MQ=255)
tggttggtAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCAGGCCTTTTTCAGGCTGa < 1:2838400/70‑1 (MQ=255)
gttggtAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGCTGAggg > 1:1823007/1‑71 (MQ=255)
ttggtAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGt < 1:575450/71‑1 (MQ=255)
gtAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGac < 1:617353/71‑1 (MQ=255)
gtAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGac > 1:3437179/1‑71 (MQ=255)
gtAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGa < 1:808887/70‑1 (MQ=255)
tAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTg < 1:720221/62‑1 (MQ=255)
tAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGac < 1:2129614/70‑1 (MQ=255)
aTGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGc > 1:3661787/1‑58 (MQ=255)
ttgtCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCttttt > 1:915819/1‑50 (MQ=255)
gtCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCgg > 1:391308/1‑41 (MQ=255)
gtCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGcc < 1:231652/70‑1 (MQ=255)
gtCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGc > 1:4051022/1‑69 (MQ=255)
gcTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCtttt < 1:2829127/41‑1 (MQ=255)
gcTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCAc > 1:2893986/1‑71 (MQ=255)
ccTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAggg < 1:545726/48‑1 (MQ=255)
tctTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGa > 1:4361409/1‑43 (MQ=255)
ctTTGTTGAGATACATCGGAATCCCGGCCTTTTTCa > 1:1214659/1‑36 (MQ=255)
ctTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGc > 1:792910/1‑39 (MQ=255)
tttGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAggg < 1:3898920/44‑1 (MQ=255)
tttGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAAt > 1:3927784/1‑69 (MQ=255)
tttGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAAt < 1:1488310/69‑1 (MQ=255)
tgttgAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGa < 1:430109/69‑1 (MQ=255)
tgAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGa > 1:1455479/1‑69 (MQ=255)
gagaTACATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCaa < 1:2485354/61‑1 (MQ=255)
aTACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATaaag < 1:586780/70‑1 (MQ=255)
tACATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATaaagca < 1:1553976/71‑1 (MQ=255)
tACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATaaagca < 1:3353625/71‑1 (MQ=255)
tACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATaaagca < 1:265438/71‑1 (MQ=255)
tACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATaaagca < 1:960873/71‑1 (MQ=255)
aCATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATaaagca > 1:3826369/1‑70 (MQ=255)
|
CGCCAATCATCATGTTCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCA > W3110S.gb/4392021‑4392156
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
