Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F17 I0 R1
|
87 |
56.5 |
4283937 |
98.6% |
4223961 |
62.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
647,863 |
A→G |
9.1% |
M311T (ATG→ACG) |
citF ← |
citrate lyase, citrate‑ACP transferase (alpha) subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 647,863 | 0 | A | G | 9.1%
| 116.0
/ 4.7
| 44 | M311T (ATG→ACG) | citF | citrate lyase, citrate‑ACP transferase (alpha) subunit |
| Reads supporting (aligned to +/- strand): ref base A (17/23); new base G (2/2); total (19/25) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 |
GCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGA > W3110S.gb/647794‑647932
|
gcagcaTGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGTTCAACCAt < 1:3784602/71‑1 (MQ=255)
gcagcaTGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCAt > 1:2285424/1‑71 (MQ=255)
agcaTGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCAtcg > 1:3305938/1‑71 (MQ=255)
agcaTGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCAtcg > 1:1666418/1‑71 (MQ=255)
gCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCgg < 1:604675/71‑1 (MQ=255)
cTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGt < 1:2164206/71‑1 (MQ=255)
tGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGt > 1:2039768/1‑70 (MQ=255)
tCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTaa > 1:720967/1‑70 (MQ=255)
tCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTaa > 1:909119/1‑70 (MQ=255)
tCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTaa < 1:1287654/70‑1 (MQ=255)
aaaGCTCTGCAGATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTa > 1:1665193/1‑67 (MQ=255)
ctctGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTAATACCGcc < 1:608713/71‑1 (MQ=255)
tGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAg > 1:2992163/1‑71 (MQ=255)
gCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGcc < 1:2868206/67‑1 (MQ=255)
cacaTCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCaa < 1:652245/68‑1 (MQ=255)
cacaTCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCaa < 1:734307/68‑1 (MQ=255)
acaTCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGc < 1:3648255/71‑1 (MQ=255)
tCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATAcc < 1:1146306/59‑1 (MQ=255)
ccAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCAtc > 1:2599201/1‑44 (MQ=255)
agcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAggg > 1:4079433/1‑64 (MQ=255)
gcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCAtcgt < 1:117714/43‑1 (MQ=255)
gcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCg > 1:1204566/1‑71 (MQ=255)
cagTTTGCGGATCAGACTTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCgg < 1:52426/71‑1 (MQ=255)
cagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCAtcgt < 1:934285/42‑1 (MQ=255)
cagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCgg > 1:2099627/1‑47 (MQ=255)
tGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGcgcg > 1:3185145/1‑70 (MQ=255)
tGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGcgcg > 1:311483/1‑70 (MQ=255)
tGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGcgcg < 1:391719/70‑1 (MQ=255)
gATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGaa < 1:2084509/57‑1 (MQ=255)
aTCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGt < 1:1230511/38‑1 (MQ=255)
aTCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATc < 1:3694905/71‑1 (MQ=255)
tCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATcg < 1:2823518/71‑1 (MQ=255)
cAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAAt < 1:3226880/39‑1 (MQ=255)
cTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTa < 1:1323471/71‑1 (MQ=255)
ttttCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGaa > 1:1599675/1‑58 (MQ=255)
tttCGTGCAGGTCAACCGTCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACg > 1:3318870/1‑70 (MQ=255)
gTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCAtt < 1:642915/70‑1 (MQ=255)
cAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTg < 1:1961871/70‑1 (MQ=255)
tCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTc < 1:379573/71‑1 (MQ=255)
cAACCGTCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTc > 1:1449713/1‑70 (MQ=255)
aaCCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGTCGCGAATATCGCGGCTACGCATTTTGTCTTc > 1:3626529/1‑69 (MQ=255)
aaCCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTcc < 1:1550680/70‑1 (MQ=255)
aaCCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTc > 1:272064/1‑69 (MQ=255)
cATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGa < 1:3889959/71‑1 (MQ=255)
|
GCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGA > W3110S.gb/647794‑647932
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
