Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
647,863 |
A→G |
M311T (ATG→ACG) |
citF ← |
citrate lyase, citrate‑ACP transferase (alpha) subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 647,863 | 0 | A | G | 100.0%
| 189.1
/ NA
| 51 | M311T (ATG→ACG) | citF | citrate lyase, citrate‑ACP transferase (alpha) subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (34/17); total (34/17) |
AAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAA > W3110S.gb/647808‑647891
|
aaaGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGtcgtcg > 1:3406068/1‑62 (MQ=255)
aaaGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGtcgtcg > 1:3310298/1‑62 (MQ=255)
aaaGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGtcgtcg > 1:3258094/1‑62 (MQ=255)
aaaGCTCTGCACATCCAGCAGTTTACGGATCAGACCTTTTTCGTGCAGGTCAACCGtcgtcg > 1:3368935/1‑62 (MQ=255)
aaGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTcgc > 1:3525537/1‑62 (MQ=255)
aaGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTcgc > 1:3256263/1‑62 (MQ=255)
aaGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTcgc > 1:2434274/1‑62 (MQ=255)
aGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTcgc < 1:3412799/61‑1 (MQ=255)
aGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTcgc < 1:901314/61‑1 (MQ=255)
aGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTcgc < 1:4467301/61‑1 (MQ=255)
aGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTcgc < 1:1545034/61‑1 (MQ=255)
aGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTcgc < 1:1599440/61‑1 (MQ=255)
aGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTcgc < 1:2505993/61‑1 (MQ=255)
aGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTcgc < 1:2500456/61‑1 (MQ=255)
ctGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTaa < 1:1732441/62‑1 (MQ=255)
ctGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTaa < 1:4464531/62‑1 (MQ=255)
ctGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTaa < 1:14929/62‑1 (MQ=255)
ctGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTaa < 1:683782/62‑1 (MQ=255)
agcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGt > 1:4139307/1‑50 (MQ=255)
agcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGt > 1:3096571/1‑50 (MQ=255)
agcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGt > 1:1226193/1‑50 (MQ=255)
agcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGt > 1:4376200/1‑50 (MQ=255)
agcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGt > 1:2695201/1‑50 (MQ=255)
agcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGt > 1:4663560/1‑50 (MQ=255)
agcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGt > 1:468199/1‑50 (MQ=255)
agcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGt > 1:2224124/1‑50 (MQ=255)
agcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGt > 1:4834/1‑50 (MQ=255)
agcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGt > 1:563160/1‑50 (MQ=255)
agcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGt > 1:1304973/1‑50 (MQ=255)
agcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGt > 1:1251497/1‑50 (MQ=255)
tttGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGt > 1:725107/1‑45 (MQ=255)
ttGCGGATCAGACCTTTTTCGTGCAGGTCAACCGtcg < 1:4077949/37‑1 (MQ=255)
ttGCGGATCAGACCTTTTTCGTGCAGGTCAACCGtcg < 1:1233483/37‑1 (MQ=255)
ttGCGGATCAGACCTTTTTCGTGCAGGTCAACCGtcg < 1:4046151/37‑1 (MQ=255)
ttGCGGATCAGACCTTTTTCGTGCAGGTCAACCGtcg < 1:235928/37‑1 (MQ=255)
ttGCGGATCAGACCTTTTTCGTGCAGGTCAACCGtcg < 1:2395832/37‑1 (MQ=255)
ttGCGGATCAGACCTTTTTCATGCAGGTCAACCGTCGTCGCGGTAATACCGCCAAGGGCGaa > 1:4511858/1‑62 (MQ=255)
tGCGGATCAGACCTTTTTCGTGCAGGTCAACcgtcgt < 1:688365/37‑1 (MQ=255)
tGCGGATCAGACCTTTTTCGTGCAGGTCAACcgtcgt < 1:1359271/37‑1 (MQ=255)
tGCGGATCAGACCTTTTTCGTGCAGGTCAACcgtcgt < 1:600599/37‑1 (MQ=255)
tGCGGATCAGACCTTTTTCGTGCAGGTCAACcgtcgt < 1:2591632/37‑1 (MQ=255)
tGCGGATCAGACCTTTTTCGTGCAGGTCAACCGtcg < 1:914149/36‑1 (MQ=255)
tCAGACCTTTTTCGTGCAGGTCAGCCGTCGTCGCGGt > 1:4299941/1‑37 (MQ=37)
tCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCgg > 1:1271960/1‑36 (MQ=255)
tCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGt > 1:3072203/1‑37 (MQ=255)
cAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTa > 1:4490172/1‑37 (MQ=255)
cAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTa > 1:2636737/1‑37 (MQ=255)
cAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTa > 1:2924235/1‑37 (MQ=255)
cAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTa > 1:2119040/1‑37 (MQ=255)
cAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTa > 1:1535913/1‑37 (MQ=255)
cAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTa > 1:3162492/1‑37 (MQ=255)
cAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTa > 1:3742525/1‑37 (MQ=255)
cAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTa > 1:713885/1‑37 (MQ=255)
cAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTa > 1:1284170/1‑37 (MQ=255)
cAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTa > 1:3922328/1‑37 (MQ=255)
|
AAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAA > W3110S.gb/647808‑647891
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
