Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F17 I0 R1
|
87 |
56.5 |
4283937 |
98.6% |
4223961 |
62.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,306,434 |
G→A |
9.6% |
L428L (CTG→TTG) |
deaD ← |
ATP‑dependent RNA helicase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,306,434 | 0 | G | A | 9.6%
| 157.7
/ 5.7
| 52 | L428L (CTG→TTG) | deaD | ATP‑dependent RNA helicase |
| Reads supporting (aligned to +/- strand): ref base G (23/24); new base A (2/3); total (25/27) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTCAGC > W3110S.gb/3306365‑3306498
|
cACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGt > 1:429863/1‑71 (MQ=255)
aCGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGt < 1:1648174/70‑1 (MQ=255)
aCGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTg < 1:3980338/71‑1 (MQ=255)
aCGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCa < 1:1757376/71‑1 (MQ=255)
cATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTg < 1:1412045/59‑1 (MQ=255)
aTCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGt < 1:1105951/71‑1 (MQ=255)
ggCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGttt < 1:2996063/70‑1 (MQ=255)
ggCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGttt > 1:112298/1‑70 (MQ=255)
ggCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTc > 1:374572/1‑71 (MQ=255)
cgcATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCga < 1:1422997/71‑1 (MQ=255)
gcATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCgag < 1:3178621/71‑1 (MQ=255)
gcATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCgag < 1:2466394/71‑1 (MQ=255)
cATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCgaga < 1:2384717/71‑1 (MQ=255)
aTCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGAt < 1:1909155/71‑1 (MQ=255)
cTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATc > 1:3943836/1‑70 (MQ=255)
cTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATc > 1:1653875/1‑70 (MQ=255)
tGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGc < 1:3176515/48‑1 (MQ=255)
tGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGtt > 1:1004324/1‑61 (MQ=255)
tGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATc > 1:2790810/1‑69 (MQ=255)
tGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGACGCAGCCAGAGTTTc > 1:1895819/1‑63 (MQ=255)
ggCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCa > 1:1315018/1‑70 (MQ=255)
ggCGGTACGATCAGAGTACGTTCAACCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCg > 1:2101751/1‑63 (MQ=255)
gCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATcc < 1:2575368/68‑1 (MQ=255)
ggTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGttt < 1:140410/58‑1 (MQ=255)
gATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCagag > 1:2126283/1‑50 (MQ=255)
gATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGttt > 1:2263921/1‑53 (MQ=255)
aTCAGAGTACGTTGACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGtt > 1:1996515/1‑51 (MQ=255)
aTCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCagag < 1:3219214/49‑1 (MQ=255)
cAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCtt > 1:1889282/1‑71 (MQ=255)
cAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCtt > 1:566783/1‑71 (MQ=255)
cAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCtt > 1:3638528/1‑71 (MQ=255)
cAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCtt > 1:1999866/1‑71 (MQ=255)
cAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCt > 1:2702853/1‑70 (MQ=255)
cccTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTca > 1:1958522/1‑60 (MQ=255)
cccTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTg < 1:2090610/71‑1 (MQ=255)
tgCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGAt < 1:2654824/38‑1 (MQ=255)
tgCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAttt > 1:613140/1‑71 (MQ=255)
tgCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAtt < 1:2302594/70‑1 (MQ=255)
tgCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAt < 1:3261104/69‑1 (MQ=255)
ccATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTTAAtttt < 1:4127155/70‑1 (MQ=255)
cATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGc > 1:4177974/1‑71 (MQ=255)
cATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGc > 1:1062318/1‑71 (MQ=255)
aTTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTg < 1:3731547/62‑1 (MQ=255)
ttttCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCAc < 1:2235207/45‑1 (MQ=255)
ttttCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCAc < 1:117174/45‑1 (MQ=255)
ttttCACCAGTGCCGCAGCCAGAGTTTCCAGATcc < 1:10311/35‑1 (MQ=37)
tttCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAg > 1:143385/1‑36 (MQ=255)
tttCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCATTCGGCTGAAttt > 1:3843767/1‑65 (MQ=255)
tttCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTATTCACCTTCAGCAGTCGGCTGAATTTTGCt > 1:4102680/1‑69 (MQ=255)
ttCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTcag < 1:2909394/49‑1 (MQ=255)
ttCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTT‑CTcag < 1:1406810/70‑1 (MQ=255)
tCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTcag < 1:3291400/70‑1 (MQ=255)
cagcagTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTcagc < 1:1239146/70‑1 (MQ=255)
|
CACGTTTCGGACGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTCAGC > W3110S.gb/3306365‑3306498
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
