Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,306,434 |
G→A |
L428L (CTG→TTG) |
deaD ← |
ATP‑dependent RNA helicase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,306,434 | 0 | G | A | 100.0%
| 96.2
/ NA
| 30 | L428L (CTG→TTG) | deaD | ATP‑dependent RNA helicase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/10); total (20/10) |
GCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTT > W3110S.gb/3306383‑3306491
|
gcgcATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGc > 1:2699259/1‑58 (MQ=255)
gcgcATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCa > 1:3690660/1‑59 (MQ=255)
gcgcATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCa > 1:3156513/1‑59 (MQ=255)
gcgcATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCa > 1:2147325/1‑59 (MQ=255)
gcgcATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCa > 1:1903275/1‑59 (MQ=255)
gcgcATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCa > 1:3850897/1‑59 (MQ=255)
cTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGtt > 1:3406339/1‑62 (MQ=255)
cTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGtt > 1:2818845/1‑62 (MQ=255)
cTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGtt > 1:1288133/1‑62 (MQ=255)
cTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGtt > 1:4455262/1‑62 (MQ=255)
ggTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCga < 1:1578121/61‑1 (MQ=255)
ggTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCga < 1:1331982/61‑1 (MQ=255)
ggTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCga < 1:3777174/61‑1 (MQ=255)
ggTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCga < 1:3016454/61‑1 (MQ=255)
ggTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCga < 1:3276033/61‑1 (MQ=255)
gTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCgaga < 1:1721597/62‑1 (MQ=255)
gTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCgaga < 1:1369465/62‑1 (MQ=255)
gTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCgaga < 1:4156108/62‑1 (MQ=255)
gTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCgaga < 1:3139593/62‑1 (MQ=255)
aTCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAg < 1:387525/62‑1 (MQ=255)
gagTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGctc > 1:748494/1‑61 (MQ=255)
gagTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGctc > 1:4328560/1‑61 (MQ=255)
gagTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGctc > 1:996327/1‑61 (MQ=255)
gagTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGctc > 1:1045097/1‑61 (MQ=255)
gagTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGctc > 1:2344594/1‑61 (MQ=255)
gagTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGctc > 1:1213259/1‑61 (MQ=255)
gagTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGctc > 1:1102971/1‑61 (MQ=255)
tACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTc > 1:1808428/1‑61 (MQ=255)
tACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCa > 1:52463/1‑62 (MQ=255)
tACGTTCACCCTGTGCCATTTTAAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTc > 1:3850547/1‑61 (MQ=255)
agcaaTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAttt < 1:3333416/61‑1 (MQ=255)
agcaaTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAttt < 1:3795144/61‑1 (MQ=255)
agcaaTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAttt < 1:1902790/61‑1 (MQ=255)
agcaaTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAttt < 1:1346573/61‑1 (MQ=255)
agcaaTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAttt < 1:4015525/61‑1 (MQ=255)
agcaaTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAttt < 1:4667266/61‑1 (MQ=255)
agcaaTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAttt < 1:541389/61‑1 (MQ=255)
gcaaTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAtttt < 1:3206314/61‑1 (MQ=255)
gcaaTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAtttt < 1:3903526/61‑1 (MQ=255)
gcaaTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAtttt < 1:1049738/61‑1 (MQ=255)
|
GCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTT > W3110S.gb/3306383‑3306491
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
