Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F17 I0 R1
|
87 |
56.5 |
4283937 |
98.6% |
4223961 |
62.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,477,260 |
G→A |
12.1% |
intergenic (‑45/+236) |
pyrL ← / ← yjgH |
pyrBI operon leader peptide/predicted mRNA endoribonuclease |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,477,260 | 0 | G | A | 12.1%
| 168.3
/ 12.2
| 58 | intergenic (‑45/+236) | pyrL/yjgH | pyrBI operon leader peptide/predicted mRNA endoribonuclease |
| Reads supporting (aligned to +/- strand): ref base G (25/26); new base A (4/3); total (29/29) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 |
ACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTA > W3110S.gb/4477190‑4477326
|
aCAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAg < 1:2987456/71‑1 (MQ=255)
aCAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAg < 1:3260861/71‑1 (MQ=255)
aCAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAg < 1:1713715/71‑1 (MQ=255)
cAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAgc > 1:3859729/1‑71 (MQ=255)
cAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAg < 1:598693/70‑1 (MQ=255)
aaaaTGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAgcg > 1:4179631/1‑71 (MQ=255)
aaaaTGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAgcg > 1:1733894/1‑71 (MQ=255)
aTGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAgcggc > 1:107669/1‑70 (MQ=255)
cGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAg > 1:967119/1‑71 (MQ=255)
gAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAg > 1:2408684/1‑70 (MQ=255)
gAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAg > 1:3382938/1‑70 (MQ=255)
cacacTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCAtt > 1:1063209/1‑71 (MQ=255)
cacacTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCAtt > 1:3484954/1‑71 (MQ=255)
cacacTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCAtt > 1:589269/1‑71 (MQ=255)
acacTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCAttt < 1:3490389/71‑1 (MQ=255)
acacTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCAttt < 1:309578/71‑1 (MQ=255)
cacTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTg < 1:4148331/71‑1 (MQ=255)
tGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGc < 1:2531557/63‑1 (MQ=255)
cATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCa < 1:297773/55‑1 (MQ=255)
cATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCa < 1:2762312/55‑1 (MQ=255)
tACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATAtt > 1:4268373/1‑71 (MQ=255)
cATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCAtt > 1:1133483/1‑57 (MQ=255)
cATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATAtttt > 1:67857/1‑71 (MQ=255)
cATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATAtttt > 1:1066989/1‑71 (MQ=255)
aTCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCAttt < 1:947274/57‑1 (MQ=255)
aTCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCAttt < 1:779490/57‑1 (MQ=255)
aTCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCAttt < 1:680971/57‑1 (MQ=255)
aTCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTg > 1:2784959/1‑71 (MQ=255)
tcctccCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGt < 1:3066685/71‑1 (MQ=255)
ctccCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCa < 1:2325773/71‑1 (MQ=255)
gCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCa > 1:3543030/1‑70 (MQ=255)
aaaTTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCAttt > 1:1281316/1‑46 (MQ=255)
aaaTTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAAc > 1:2683637/1‑71 (MQ=255)
aaTTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACg > 1:143509/1‑71 (MQ=255)
aTTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCAtt > 1:796171/1‑43 (MQ=255)
aTTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTg < 1:2618290/45‑1 (MQ=255)
aTTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACgg < 1:2050006/71‑1 (MQ=255)
aTTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACgg > 1:3917846/1‑71 (MQ=255)
aTTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACgg > 1:389089/1‑71 (MQ=255)
aTTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACgg > 1:471436/1‑71 (MQ=255)
aTTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACgg > 1:2419202/1‑71 (MQ=255)
ttGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGc < 1:3392463/39‑1 (MQ=255)
ttGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAata < 1:4248189/52‑1 (MQ=255)
ttGTCCGGCATTATACTCATCGTAAGAAGCGGCGCAAGCATTTGa > 1:2738704/1‑45 (MQ=255)
tCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGttt < 1:3584028/70‑1 (MQ=255)
ccGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGa > 1:2225478/1‑41 (MQ=255)
cGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCa < 1:3498248/55‑1 (MQ=255)
cGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTAtt > 1:3059416/1‑71 (MQ=255)
cATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTg > 1:1643510/1‑70 (MQ=255)
ttATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATTTTTCGt < 1:1832591/48‑1 (MQ=255)
ttATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGt < 1:1867300/48‑1 (MQ=255)
ttATACTCATCGTCAGAAACTGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTa < 1:2280541/64‑1 (MQ=255)
ttATACTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTa < 1:1429580/64‑1 (MQ=255)
tataCTCATCGTCAGAAGCGGCGCAAGCATTTGATGc < 1:4249947/37‑1 (MQ=255)
tataCTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGt < 1:1080976/47‑1 (MQ=255)
ataCTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAgcgc < 1:617724/52‑1 (MQ=255)
taCTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTaa < 1:1514488/71‑1 (MQ=255)
tCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAg > 1:2024250/1‑71 (MQ=255)
tCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAAc < 1:3884665/49‑1 (MQ=255)
tCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTc < 1:2479052/70‑1 (MQ=255)
tCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCa < 1:3186584/71‑1 (MQ=255)
tCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCa < 1:4011402/46‑1 (MQ=255)
gTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAgc > 1:2250016/1‑41 (MQ=255)
gTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGt > 1:4133516/1‑67 (MQ=255)
gTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGt > 1:3551531/1‑67 (MQ=255)
aGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGta > 1:1300226/1‑71 (MQ=255)
|
ACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTA > W3110S.gb/4477190‑4477326
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
