Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,477,260 |
G→A |
intergenic (‑45/+236) |
pyrL ← / ← yjgH |
pyrBI operon leader peptide/predicted mRNA endoribonuclease |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,477,260 | 0 | G | A | 100.0%
| 128.2
/ NA
| 41 | intergenic (‑45/+236) | pyrL/yjgH | pyrBI operon leader peptide/predicted mRNA endoribonuclease |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (30/11); total (30/11) |
ACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATT > W3110S.gb/4477202‑4477313
|
acacacTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAacgg > 1:4300537/1‑62 (MQ=255)
acacacTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAacgg > 1:1439625/1‑62 (MQ=255)
cacacTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAacggc < 1:2539889/62‑1 (MQ=255)
cacacTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAacggc < 1:3352463/62‑1 (MQ=255)
cacacTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAacggc < 1:1418474/62‑1 (MQ=255)
acacTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAacggc < 1:3862643/61‑1 (MQ=255)
acTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCa > 1:1632227/1‑62 (MQ=255)
acTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCa > 1:1730308/1‑62 (MQ=255)
acTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATAGTCAGAAACGGCGCa > 1:3576478/1‑62 (MQ=255)
acTGAACCATACATCCTCCCGGCAAATTGTCCGGCATGATACTCATCGTCAGAAACGGCGCa > 1:1709929/1‑62 (MQ=255)
ccATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCAt < 1:380402/61‑1 (MQ=255)
ccATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCAt < 1:4048859/61‑1 (MQ=255)
ccATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCAt < 1:4227610/61‑1 (MQ=255)
ccATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCAt < 1:1002447/61‑1 (MQ=255)
ccATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCAt < 1:260900/61‑1 (MQ=255)
ccATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCAt < 1:2594406/61‑1 (MQ=255)
ccATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCAt < 1:882444/61‑1 (MQ=255)
cATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAacgg < 1:1784834/47‑1 (MQ=255)
ccGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCa > 1:342273/1‑44 (MQ=255)
ccGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCATTTGATGCAatat > 1:1749917/1‑61 (MQ=255)
ccGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCATTTGATGCAatat > 1:2223873/1‑61 (MQ=255)
ccGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATAtt > 1:3377954/1‑62 (MQ=255)
ccGGCAAATTGTCCAGCATTATACTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATAtt > 1:759263/1‑62 (MQ=255)
cAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCAttt > 1:1326621/1‑47 (MQ=255)
cAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCAttt > 1:1148037/1‑47 (MQ=255)
cAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCAttt > 1:123725/1‑47 (MQ=255)
cAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCAttt > 1:3929710/1‑47 (MQ=255)
cAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCAttt > 1:338025/1‑47 (MQ=255)
cAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCAttt > 1:2114392/1‑47 (MQ=255)
gCATTATACTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACg > 1:38113/1‑62 (MQ=255)
cATTATACTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCaa > 1:3765879/1‑58 (MQ=255)
aCTCAACGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTAt > 1:294931/1‑61 (MQ=255)
cTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTAtt < 1:3457516/61‑1 (MQ=255)
cTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTAtt < 1:521526/61‑1 (MQ=255)
cTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTAtt < 1:1176839/61‑1 (MQ=255)
cTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTCTGTCAGCGCAAACGGTTTAtt < 1:1198399/61‑1 (MQ=255)
tCGTCAGAAACGGCGCAAGCATTTGATGCAATAtt > 1:4141816/1‑35 (MQ=255)
tCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGttt > 1:2289711/1‑54 (MQ=255)
tCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGttt > 1:774417/1‑54 (MQ=255)
tCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGttt > 1:71302/1‑54 (MQ=255)
tCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGttt > 1:3718456/1‑54 (MQ=255)
tCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGttt > 1:1494529/1‑54 (MQ=255)
tCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGaa > 1:2839436/1‑61 (MQ=255)
tCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGaa > 1:1532404/1‑61 (MQ=255)
gTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAAtt > 1:4135944/1‑61 (MQ=255)
gTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAAtt > 1:617910/1‑61 (MQ=255)
gTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAAtt > 1:1300599/1‑61 (MQ=255)
gTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAAtt > 1:729724/1‑61 (MQ=255)
|
ACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATT > W3110S.gb/4477202‑4477313
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
