Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R1
|
2169 |
63.0 |
3952800 |
76.2% |
3012033 |
62.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,309,899 |
G→A |
53.3% |
R31R (CGC→CGT) |
yefM ← |
antitoxin of the YoeB‑YefM toxin‑antitoxin system |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,309,899 | 0 | G | A | 53.3%
| 5.2
/ 79.2
| 60 | R31R (CGC→CGT) | yefM | antitoxin of the YoeB‑YefM toxin‑antitoxin system |
| Reads supporting (aligned to +/- strand): ref base G (15/13); new base A (12/20); total (27/33) |
| Fisher's exact test for biased strand distribution p-value = 2.99e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTT > minE/1309829‑1309969
|
gTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTgg > 1:2392121/1‑71 (MQ=255)
gTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTgg < 1:2303759/71‑1 (MQ=255)
aGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGc > 1:172098/1‑70 (MQ=255)
gATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGc > 1:1392467/1‑69 (MQ=255)
gATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGc > 1:3142405/1‑69 (MQ=255)
gCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGtaat < 1:1938994/71‑1 (MQ=255)
gCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGtaa > 1:3373805/1‑70 (MQ=255)
tctcTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGa < 1:616177/62‑1 (MQ=255)
ccAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCggg > 1:2630721/1‑71 (MQ=255)
aGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGc < 1:2671103/71‑1 (MQ=255)
aGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGc < 1:3198811/71‑1 (MQ=255)
cGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGAt < 1:3375388/63‑1 (MQ=255)
gAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTACATTCTGGCGAGTAATAAGGATCGGGGCATg < 1:3128761/71‑1 (MQ=255)
aGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGa < 1:692245/71‑1 (MQ=255)
gTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGtaata < 1:3220600/55‑1 (MQ=255)
gTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGtaata < 1:3215993/55‑1 (MQ=255)
gTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGa < 1:3035727/70‑1 (MQ=255)
gTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGAt < 1:1275924/71‑1 (MQ=255)
tGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGAt > 1:1465473/1‑69 (MQ=255)
tGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCt > 1:2163195/1‑71 (MQ=255)
tATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTc < 1:3616012/71‑1 (MQ=255)
aTTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAg > 1:1547878/1‑52 (MQ=255)
aTTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTc > 1:1915203/1‑70 (MQ=255)
aTTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTc > 1:329914/1‑70 (MQ=255)
aTTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTc > 1:829077/1‑70 (MQ=255)
ttcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCaa < 1:2221060/71‑1 (MQ=255)
tcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAAc > 1:3280305/1‑71 (MQ=255)
cttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCAt > 1:761665/1‑60 (MQ=255)
cttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCAt > 1:2552350/1‑60 (MQ=255)
cGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGc < 1:2537929/70‑1 (MQ=255)
gAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCtt < 1:1225046/71‑1 (MQ=255)
aGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCttt < 1:3131965/71‑1 (MQ=255)
gTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGAt < 1:334803/46‑1 (MQ=255)
gTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGAt < 1:1676780/46‑1 (MQ=255)
tGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtc > 1:949143/1‑70 (MQ=255)
aCATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtcat > 1:3335116/1‑70 (MQ=255)
cATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtcatca < 1:137649/71‑1 (MQ=255)
aTCAGATCACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCAt < 1:156310/49‑1 (MQ=255)
tCAGAACACTAGCCTCTCCATTCTGACGTGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtcatc < 1:3027107/68‑1 (MQ=255)
tCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtcatca < 1:239735/69‑1 (MQ=255)
tCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTcatcat < 1:3259485/70‑1 (MQ=255)
cAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAttg > 1:2770689/1‑71 (MQ=255)
aGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAttg < 1:672221/70‑1 (MQ=255)
aaCACAAGCCTCTCCATTCTGGCGAGTAATAAGGAt > 1:1385683/1‑36 (MQ=255)
aaCACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGc < 1:1394240/42‑1 (MQ=255)
aaCACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtcatc < 1:1317114/64‑1 (MQ=255)
acacAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGc < 1:2528690/41‑1 (MQ=255)
acacAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGc > 1:1921347/1‑71 (MQ=255)
cacaAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCAt > 1:2849870/1‑42 (MQ=255)
cacaAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGcc < 1:203904/71‑1 (MQ=255)
caAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGa > 1:226798/1‑71 (MQ=255)
aaGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAttgttg < 1:1669237/66‑1 (MQ=255)
gCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtcatca > 1:109406/1‑58 (MQ=255)
gCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACa > 1:803732/1‑70 (MQ=255)
tctcCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGcc < 1:3465832/63‑1 (MQ=255)
tcCATTCTGGCGAGTAATAAGGATCGGGGCATGAta > 1:1616365/1‑35 (MQ=255)
tcCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAAtt < 1:2021750/69‑1 (MQ=255)
cATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGa > 1:2611727/1‑71 (MQ=255)
aTTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGAc > 1:1789573/1‑71 (MQ=255)
aTTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAttgttg < 1:3687286/56‑1 (MQ=255)
ttCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAAc < 1:3786606/38‑1 (MQ=255)
tCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGa > 1:858404/1‑58 (MQ=255)
gCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCtt < 1:693974/71‑1 (MQ=255)
|
GTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTT > minE/1309829‑1309969
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
