Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R1
|
2169 |
63.0 |
3952800 |
76.2% |
3012033 |
62.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,753,067 |
G→A |
27.9% |
V143V (GTG→GTA) |
yfiQ → |
fused predicted acyl‑CoA synthetase NAD(P)‑binding subunit and ATP‑binding subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,753,067 | 0 | G | A | 27.9%
| 64.4
/ 26.0
| 43 | V143V (GTG→GTA) | yfiQ | fused predicted acyl‑CoA synthetase NAD(P)‑binding subunit and ATP‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base G (17/14); new base A (6/6); total (23/20) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGG > minE/1753001‑1753136
|
gCTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCGa > 1:1973452/1‑71 (MQ=255)
cTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCg < 1:3036660/69‑1 (MQ=255)
cTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGa < 1:2454159/70‑1 (MQ=255)
ggACCAAACAGTCTGGGTTTACTGGCTCCCTGCCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGAtt < 1:3513201/69‑1 (MQ=255)
aCCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAAc < 1:3299837/71‑1 (MQ=255)
cAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAAcgc > 1:117596/1‑71 (MQ=255)
cAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCGa > 1:2130518/1‑63 (MQ=255)
cAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCGATTAAAcgc > 1:2664600/1‑71 (MQ=255)
aaCAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCgg > 1:3877998/1‑71 (MQ=255)
aaCAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCgg > 1:2034158/1‑71 (MQ=255)
aCAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCgg > 1:236683/1‑70 (MQ=255)
aGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCaa < 1:1761612/71‑1 (MQ=255)
gTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAg < 1:2234221/71‑1 (MQ=255)
gTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCGATTAAACGCGGCAAg < 1:2274366/71‑1 (MQ=255)
tCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCg < 1:189365/55‑1 (MQ=255)
cTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCt > 1:3646830/1‑71 (MQ=255)
cTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCt > 1:935392/1‑71 (MQ=255)
ggTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGc < 1:1463202/71‑1 (MQ=255)
ttACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGtt > 1:484141/1‑71 (MQ=255)
ttACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGtt > 1:1759806/1‑71 (MQ=255)
tACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCtgtg < 1:1875842/44‑1 (MQ=255)
gCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCGATTAAACGCGGCAAGCTGGCGTTTAtt < 1:539261/69‑1 (MQ=255)
gCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCGATTAAACGCGGCAAGCTGGCGTTTATTTc < 1:183813/71‑1 (MQ=255)
cTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCg > 1:881026/1‑71 (MQ=255)
tCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTg > 1:3159929/1‑58 (MQ=255)
tCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCg > 1:1479939/1‑70 (MQ=255)
cTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAAt > 1:350053/1‑71 (MQ=255)
cTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAAt < 1:1889190/71‑1 (MQ=255)
ggCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCGATTAAACGCGGCAAGCTGGCg > 1:1115372/1‑56 (MQ=255)
gCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTa < 1:366714/38‑1 (MQ=255)
aGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCt > 1:56571/1‑70 (MQ=255)
aGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTg < 1:1319935/71‑1 (MQ=255)
ggTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGc < 1:3580330/46‑1 (MQ=255)
ggTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCt > 1:701764/1‑69 (MQ=255)
gTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTc > 1:1389824/1‑58 (MQ=255)
cTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCg > 1:2948122/1‑70 (MQ=255)
aaTGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGtctc > 1:331631/1‑71 (MQ=255)
gCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCa < 1:2413528/53‑1 (MQ=255)
tttCGCCTGTACCGATTAAACGCGGCAAGCTGGCGTTTa > 1:1351973/1‑39 (MQ=255)
tCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCTCAATCGGCTGCCGTCTCCAACACCATcc < 1:2816161/70‑1 (MQ=255)
tCGCCTGTACCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCt > 1:1364960/1‑71 (MQ=255)
gCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTc < 1:2327211/70‑1 (MQ=255)
gCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCg < 1:2162042/71‑1 (MQ=255)
gCCTGTACCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTc < 1:2305970/70‑1 (MQ=255)
tgCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACt < 1:3120322/69‑1 (MQ=255)
tgCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTgg < 1:3718716/71‑1 (MQ=255)
|
GCTTGGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGG > minE/1753001‑1753136
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
