Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F21 I0 R1 2169 63.0 3952800 76.2% 3012033 62.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,595,538 A→T 16.5% S354T (TCT→ACT)  arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,595,5380AT16.5% 302.9 / 47.2 121S354T (TCT→ACT) arsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base A (53/48);  new base T (13/7);  total (66/55)
Fisher's exact test for biased strand distribution p-value = 3.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

GTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGA  >  minE/2595471‑2595606
                                                                   |                                                                    
gTAATTTTCTGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGc                                                                   >  1:3629754/1‑71 (MQ=255)
 tAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc                                                                  <  1:1716718/71‑1 (MQ=255)
  gaTTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCg                                                                 >  1:2310222/2‑71 (MQ=255)
   aTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAAGGCCAGATGCCGt                                                                >  1:3657593/1‑71 (MQ=255)
   aTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCg                                                                 <  1:3632583/70‑1 (MQ=255)
   aTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGt                                                                >  1:2282745/1‑71 (MQ=255)
      ttCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgct                                                             >  1:1318215/1‑71 (MQ=255)
      ttCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgct                                                             >  1:1834918/1‑71 (MQ=255)
       tCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgctg                                                            >  1:1079007/1‑71 (MQ=255)
       tCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGtactc                                                            >  1:1335928/1‑70 (MQ=255)
        cGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGtactc                                                            <  1:1014757/70‑2 (MQ=255)
         ggTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgctgc                                                           >  1:1098596/1‑70 (MQ=255)
         ggTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGcc                                                          <  1:2138816/71‑1 (MQ=255)
          gTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCa                                                         <  1:3379826/71‑1 (MQ=255)
          gTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCa                                                         <  1:3081292/71‑1 (MQ=255)
           tCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtg                                                               <  1:1221579/64‑1 (MQ=255)
           tCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtg                                                               <  1:3699881/64‑1 (MQ=255)
            cccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgctg                                                            >  1:3733789/1‑66 (MQ=255)
            cccAAATCGAAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCAt                                                        >  1:556/1‑70 (MQ=255)
              cAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGAt                                                                     >  1:1126735/1‑55 (MQ=255)
               aaaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAAt                                                    >  1:2797251/1‑71 (MQ=255)
               aaaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAt                                                    >  1:3493069/1‑71 (MQ=255)
                aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgc                                                              >  1:3524820/1‑60 (MQ=255)
                aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgc                                                              >  1:96736/1‑60 (MQ=255)
                aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                                   <  1:2865012/71‑1 (MQ=255)
                aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                                   <  1:2090554/71‑1 (MQ=255)
                aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                                   >  1:2405114/1‑71 (MQ=255)
                aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                                   >  1:1919377/1‑71 (MQ=255)
                aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                                   >  1:899769/1‑71 (MQ=255)
                aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                                   >  1:1132875/1‑71 (MQ=255)
                aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                                   >  1:1673561/1‑71 (MQ=255)
                aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGta                                                               >  1:743546/1‑58 (MQ=255)
                aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAtc                                                   >  1:453421/1‑70 (MQ=255)
                aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAtc                                                   >  1:1814704/1‑70 (MQ=255)
                 aTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCaa                                                     <  1:3923911/68‑1 (MQ=255)
                   cGCAGCCAATCACATTGGCATAAACCATCGGTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGa                                                 >  1:810901/1‑70 (MQ=255)
                     cAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACaa                                              <  1:2174292/71‑1 (MQ=255)
                      aGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAc                                             <  1:1588070/71‑1 (MQ=255)
                       gCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGcg                                            <  1:925202/71‑1 (MQ=255)
                        ccAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcg                                            >  1:2386721/1‑70 (MQ=255)
                         gaaTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCAGCAATGGACAAcgcg                                          <  1:1149530/70‑1 (MQ=255)
                         cAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAgcgca                                          >  1:3797257/1‑70 (MQ=255)
                         cAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc                                           <  1:1894529/70‑1 (MQ=255)
                          aaTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAgcgcac                                         >  1:782482/1‑69 (MQ=255)
                          aaTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAgcgca                                          <  1:2834251/70‑2 (MQ=255)
                          aaTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc                                         <  1:301922/71‑1 (MQ=255)
                          aaTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc                                         >  1:3837660/1‑71 (MQ=255)
                           aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc                                                                  <  1:794708/45‑1 (MQ=255)
                           aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCg                                                                 >  1:3140959/1‑46 (MQ=255)
                           aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGt                                                                >  1:2633605/1‑47 (MQ=255)
                           aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCa                                                         <  1:1290411/54‑1 (MQ=255)
                           aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc                                         >  1:2994059/1‑70 (MQ=255)
                            taacaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                                   >  1:377381/3‑59 (MQ=255)
                            tCACATTGGCATAAACCATCGCTTCTTTGATAACGCTAGATGCCGTGCTGCCATCAATGGACAACGCGcc                                        >  1:3386963/1‑70 (MQ=255)
                            tCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                                   >  1:2580474/1‑59 (MQ=255)
                            tCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGcc                                        >  1:2944604/1‑70 (MQ=255)
                            tCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGcc                                        >  1:729627/1‑70 (MQ=255)
                              acaTTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCaat                                     >  1:831528/1‑70 (MQ=255)
                              acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTTCTGCCATCAATGGACAACGCGCCAAc                                     <  1:3891851/71‑1 (MQ=255)
                               caTTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCaatc                                    <  1:960083/71‑3 (MQ=255)
                               caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAcc                                    <  1:1891077/71‑1 (MQ=255)
                               caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAcc                                    <  1:573784/71‑1 (MQ=255)
                               caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAcc                                    <  1:3866880/71‑1 (MQ=255)
                                aTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa                                   <  1:2081315/71‑1 (MQ=255)
                                 ttGGCATAAACCATCGCTTCTTTGATATCGCCAGTTGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg                                  <  1:1018982/71‑1 (MQ=255)
                                 ttGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg                                  <  1:3657448/71‑1 (MQ=255)
                                  tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg                                  >  1:331902/1‑70 (MQ=255)
                                  tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg                                  >  1:2753474/1‑70 (MQ=255)
                                  tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg                                  >  1:283908/1‑70 (MQ=255)
                                      aTAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCaa                                                     <  1:3334613/47‑1 (MQ=255)
                                      aTAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCaa                                                     <  1:3172012/47‑1 (MQ=255)
                                      aTAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc                                           <  1:778934/57‑1 (MQ=255)
                                       tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc                                           >  1:2829819/1‑56 (MQ=255)
                                       tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCg                             <  1:1832053/70‑1 (MQ=255)
                                       tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGt                            >  1:3470445/1‑71 (MQ=255)
                                        aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                           >  1:3243634/1‑71 (MQ=255)
                                         aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                           <  1:62314/70‑1 (MQ=255)
                                             aTCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc                                     >  1:1860656/1‑56 (MQ=255)
                                                gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa                                   >  1:1827212/1‑55 (MQ=255)
                                                gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCatat                    <  1:1697944/70‑1 (MQ=255)
                                                gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt                   >  1:1389065/1‑71 (MQ=255)
                                                gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt                   <  1:1414431/71‑1 (MQ=255)
                                                 cttcttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgcg                                          <  1:349717/47‑1 (MQ=255)
                                                 cttcttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg                  >  1:1063083/1‑71 (MQ=255)
                                                 cttcttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg                  <  1:2641752/71‑1 (MQ=255)
                                                  ttcttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAc                               <  1:280419/57‑1 (MQ=255)
                                                    cttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGtt                <  1:3518380/70‑1 (MQ=255)
                                                    cttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGt                 <  1:1413438/69‑1 (MQ=255)
                                                    cttTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGtt                >  1:3001570/1‑70 (MQ=255)
                                                       tGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCa              <  1:1517361/69‑1 (MQ=255)
                                                        gATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata            <  1:2228124/70‑1 (MQ=255)
                                                         aTAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCat             >  1:1404703/1‑68 (MQ=255)
                                                          tAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataata         <  1:3771082/71‑1 (MQ=255)
                                                          tAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataa           >  1:3037491/1‑69 (MQ=255)
                                                           aaCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAcc                              >  1:1638895/1‑49 (MQ=255)
                                                               ccAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa                                   >  1:2593501/1‑40 (MQ=255)
                                                                cAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaa      >  1:1563804/1‑68 (MQ=255)
                                                                 aGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga    >  1:1388102/1‑69 (MQ=255)
                                                                 aGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagaga  <  1:3061942/71‑1 (MQ=255)
                                                                  gATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTc               >  1:2859084/1‑57 (MQ=255)
                                                                   |                                                                    
GTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGA  >  minE/2595471‑2595606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A