Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R1
|
2169 |
63.0 |
3952800 |
76.2% |
3012033 |
62.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,605,504 |
A→G |
23.9% |
intergenic (‑83/‑308) |
uspA ← / → yhiO |
universal stress global response regulator/predicted universal stress (ethanol tolerance) protein B |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,605,504 | 0 | A | G | 23.9%
| 115.8
/ 38.0
| 67 | intergenic (‑83/‑308) | uspA/yhiO | universal stress global response regulator/predicted universal stress (ethanol tolerance) protein B |
| Reads supporting (aligned to +/- strand): ref base A (25/26); new base G (7/9); total (32/35) |
| Fisher's exact test for biased strand distribution p-value = 7.80e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 |
AGTTGTCGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGCGACCCGGAT > minE/2605441‑2605573
|
aGTTGTCGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTGCGTgcgc < 1:3498905/71‑1 (MQ=255)
ttGTCGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTGCGTGCGCgg < 1:1792918/71‑1 (MQ=255)
ttGTCGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCgg < 1:3549497/71‑1 (MQ=255)
ttGTCGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCgg < 1:951110/71‑1 (MQ=255)
ttGTCGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCgg < 1:3343757/71‑1 (MQ=255)
gTCGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCggg > 1:1225345/1‑70 (MQ=255)
tCGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTGCGt < 1:3585/62‑1 (MQ=255)
tCGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTGCGTGCGCGGGGc < 1:3233630/71‑1 (MQ=255)
cGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTGCGTGCGCgggg > 1:1183417/1‑69 (MQ=255)
cGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTGCGTGCGCgggg > 1:92917/1‑69 (MQ=255)
gATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCt < 1:1011969/70‑1 (MQ=255)
aTGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTGCGTGCGCGGGGCTaa > 1:621440/1‑71 (MQ=255)
aTGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGTCTaa < 1:2165794/71‑1 (MQ=255)
tGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAAc > 1:337017/1‑71 (MQ=255)
gACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGt > 1:2451709/1‑57 (MQ=255)
gACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGt > 1:870586/1‑57 (MQ=255)
gACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGt > 1:2031162/1‑57 (MQ=255)
aCTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAAcc > 1:3744539/1‑70 (MQ=255)
cTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTGCg > 1:823014/1‑54 (MQ=255)
tGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCt < 1:3839152/64‑1 (MQ=255)
ggCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTGCGTGCGCGGGGCTAACCCAGc > 1:281144/1‑71 (MQ=255)
gCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAg < 1:1956480/69‑1 (MQ=255)
cAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGt < 1:3888551/71‑1 (MQ=255)
aGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTa < 1:1451055/71‑1 (MQ=255)
gCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTg < 1:595414/49‑1 (MQ=255)
gCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTaa > 1:2958301/1‑60 (MQ=255)
cTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTGCGTGCGCGGGGCTAACCCAGCCGTa > 1:2673115/1‑69 (MQ=255)
cTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTaa > 1:2950010/1‑59 (MQ=255)
cTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTa > 1:3051232/1‑69 (MQ=255)
tAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTGCGTGCGCGGGGCTa < 1:1603554/57‑1 (MQ=255)
aGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGGGAACTGCGt < 1:2951470/45‑1 (MQ=255)
gCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGa < 1:1335566/70‑1 (MQ=255)
gTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAAccc > 1:2541238/1‑57 (MQ=255)
tttCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCa < 1:3879602/71‑1 (MQ=255)
ttCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAg > 1:1670213/1‑71 (MQ=255)
cTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCa > 1:1013183/1‑54 (MQ=255)
cTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAgtg > 1:70330/1‑71 (MQ=255)
cTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAgt < 1:2913864/70‑1 (MQ=255)
cTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAgt < 1:2605836/70‑1 (MQ=255)
gtgCCGTTACGTCACCCGCGTCCTGCGAACTGCGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGtt < 1:1796126/70‑1 (MQ=255)
cGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAgtgt < 1:1428150/65‑1 (MQ=255)
gTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGAcc < 1:852897/71‑1 (MQ=255)
ttACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGtt < 1:3196860/64‑1 (MQ=255)
ttACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGAc < 1:2241418/69‑1 (MQ=255)
ttACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGAc < 1:886030/69‑1 (MQ=255)
aCGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGa > 1:635319/1‑66 (MQ=255)
aCGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATc > 1:2871342/1‑71 (MQ=255)
aCGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATc > 1:1847495/1‑71 (MQ=255)
gTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTaaa < 1:3068280/49‑1 (MQ=255)
gTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATcct > 1:3886249/1‑71 (MQ=255)
tCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATcctt < 1:272135/71‑1 (MQ=255)
tCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATcct > 1:3643296/1‑70 (MQ=255)
cACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATcctta > 1:3523187/1‑71 (MQ=255)
cgcgTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCAtcctttcctt < 1:2013333/71‑1 (MQ=255)
cgcgTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATccttacctt < 1:2017736/71‑1 (MQ=255)
cgcgTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATccttacct < 1:2937843/70‑1 (MQ=255)
gcgTCCTGCGAACTGCGTTCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATccttacctta < 1:496190/71‑1 (MQ=255)
gcgTCCTGCGAACTGCGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATcctta > 1:359422/1‑66 (MQ=255)
gcgTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGAt < 1:2820465/45‑1 (MQ=255)
cGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGCGAcc > 1:3518070/1‑71 (MQ=255)
gAACTGCGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGCGAccc < 1:110906/71‑1 (MQ=255)
gAACTACGTGCGCGGGGCTTACCCAGCCGTAAAGATTCAGTGTTAAGACCATc > 1:1993295/1‑53 (MQ=255)
gAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTtat > 1:1615210/1‑63 (MQ=255)
aCTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGCGACCCgg < 1:1378711/71‑1 (MQ=255)
aCTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGCGACCCg > 1:276241/1‑70 (MQ=255)
cTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGCGACCCGga > 1:1309540/1‑71 (MQ=255)
tACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGCGACCCGgat > 1:2233535/1‑71 (MQ=255)
aCGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGCGACCCGgat > 1:1856896/1‑70 (MQ=255)
aCGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGCGACCCGgat > 1:2267476/1‑70 (MQ=255)
aCGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGCGACCCGgat > 1:1372975/1‑70 (MQ=255)
|
AGTTGTCGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGCGACCCGGAT > minE/2605441‑2605573
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
