Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F21 I0 R1	2169	63.0	3952800	76.2%	3012033	62.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,675,295	G→A	24.3%	L60L (CTG→TTG)	gntY ←	predicted gluconate transport associated protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,675,295	0	G	A	24.3%	131.9 / 40.6	74	L60L (CTG→TTG)	gntY	predicted gluconate transport associated protein
Reads supporting (aligned to +/- strand): ref base G (27/29); new base A (9/9); total (36/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAACAAAATCGATCTCTGCATCTTCCAGGTATGGTGCGCTTAACTCATCAACATACGCGGTCAGCAGGTCAAATTTCAGGGCTGTGTCGGTGGCTTCCACAGCGTCCGGCGGACAATAAGAAACGCCACATTCAG  >  minE/2675229‑2675363

tAACAAAATCGATCTCTGCATCTTCCAGGTATGGTGCGCTTAACTCATCAACATACGCGGTCAGCAGGTCa                                                                  <  1:2317746/71‑1 (MQ=255)

tAACAAAATCGATCTCTGCATCTTCCAGGTATGGTGCGCTTAACTCATCAACATACGCGGTCAGCAGGTCa                                                                  <  1:2091591/71‑1 (MQ=255)

tAACAAAATCGATCTCTGCATCTTCCAGGTATGGTGCGCTTAACTCATCAACATACGCGGTCAGCAAGTc                                                                   >  1:3929807/1‑70 (MQ=255)

  aCAAAATCGATCTCTGCATCTTCCAGGTATGGTGCGCTTAACTCATCAACATACGCGGTCAGCAGGTCaaa                                                                >  1:642077/1‑71 (MQ=255)

   cAAAATCGATCTCTGCATCTTCCAGGTATGGTGCGCTTAACTCATCAACATACGCGGTCAGCAAGTCAAAt                                                               >  1:3283767/1‑71 (MQ=255)

    aaaaTCGATCTCTGCATCTTCCAGGTATGGTGCGCTTAACTCATCAACATACGCGGTCAGCAGGTCAAAtt                                                              >  1:2841736/1‑71 (MQ=255)

     aaaTCGATCTCTGCATCTTCCAGGTATGGTGCGCTTAACTCATCAACATACGCGGTCAGCAGGTCAAAttt                                                             >  1:3424207/1‑71 (MQ=255)

     aaaTCGATCTCTGCATCTTCCAGGTATGGTGCGCTTAACTCATCAACATACGCGGTCAGCAGGTCAAAttt                                                             <  1:582688/71‑1 (MQ=255)

      aaTCGATCTCTGCATCTTCCAGGTATGGTGCGCTTAACTCATCAACATACGCGGTCAGCAGGTCAAAttt                                                             <  1:1065570/70‑1 (MQ=255)

      aaTCGATCTCTGCATCTTCCAGGTATGGTGCGCTTAACTCATCAACATACGCGGTCAGCAGGTCAAATTTc                                                            <  1:2816786/71‑1 (MQ=255)

      aaTCGATCTCTGCATCTTCCAGGTATGGTGCGCTTAACTCATCAACATACGCGGTCAGCAGGTCAAATTTc                                                            <  1:1706791/71‑1 (MQ=255)

       aTCGATCTCTGCATCTTCCAGGTATGGTGCGCTTAACTCATCAACATACGCGGTCAGCAGGTCaa                                                                 <  1:454947/65‑1 (MQ=255)

        tCGATCTCTGCATCTTCCAGGTATGGTGCGCTTAACTCATCAACATACTCGGTCAGCAGGTCAAATTTCAg                                                          <  1:2931406/71‑1 (MQ=255)

         cGATCTCTGCATCTTCCAGGTATGGTGCGCTTAACTCATCAACATACGCGGTCAGCAGGTCAAATTTCAgg                                                         >  1:2469390/1‑71 (MQ=255)

             ctctGCATCTTCCAGGTATGGTGCGCTTAACTCATCAACATACGCGGTCAGCAGGTCAAATTTCAGGGCtg                                                     >  1:390359/1‑71 (MQ=255)

                tGCATCTTCCAGGTATGGTGCGCTTAACTCATCAACATACGCGGTCAGCAAGTCAAATTTCAGGGc                                                       <  1:913946/66‑1 (MQ=255)

                tGCATCTTCCAGGTATGGTGCGCTTAACTCATCAACATACGCGGTCAGCAAGTCAAATTTCAGGGCtgt                                                    <  1:645904/69‑1 (MQ=255)

                 gCATCTTCCAGGTATGGTGCGCTTAACTCATTAACATACGCGGTCAGCAGGTCAAATTTCAGGGCTGTGTc                                                 >  1:2775525/1‑71 (MQ=255)

                   aTCTTCCAGGTATGGTGCGCTTAACTCATCAACATACGCGGTCAGCAGGTCAAATTTCAGGGCTGTGTCgg                                               <  1:760985/71‑1 (MQ=255)

                   aTCTTCCAGGTATGGTGCGCTTAACTCATCAACATACGCGGTCAGCAAGTCAAATTTCAGGGCTGTGTCgg                                               >  1:3070291/1‑71 (MQ=255)

                    tCTTCCAGGTATGGTGCGCTTAACTCATCAACATACGCGGTCAGCAGGTCAAATTTCAGGGCCGTGTCGGt                                              >  1:1197603/1‑71 (MQ=255)

                     cTTCCAGGTATGGTGCGCTTAACTCATCAACATACGCGGTCAGCAGGTCAAATTTCa                                                           >  1:47156/1‑57 (MQ=255)

                     cTTCCAGGTATGGTGCGCTTAACTCATCAACATACGCGGTCAGCAAGTCAAATTTCAGGGCTGTGTCGGTg                                             >  1:3645930/1‑71 (MQ=255)

                        ccAGGTATGGTGCGCTTAACTCATCAACATACGCGGTCAGCAGGTCAAATTTCAGGGCTGTGTCGGTgg                                            <  1:1976417/69‑1 (MQ=255)

                          aGGTATGGTGCGCTTAACTCATCAACATACGCGGTCAGCAGGTCAAATTTCAGGGCTGTGTCGGTGGCTTc                                        <  1:1364428/71‑1 (MQ=255)

                           ggTATGGTGCGCTTAACTCATCAACATACGCGGTCAGCAAGTCAAATTTCAGGGCTGTGTCGGTGGCTTcc                                       <  1:2933787/71‑1 (MQ=255)

                            gTATGGTGCGCTTAACTCATCAACATACGCGGTCAGCAGGTCAAATTTCAGGGCTGTGTCGGTGGCTTCca                                      >  1:3378743/1‑71 (MQ=255)

                            gTATGGTGCGCTTAACTCATCAACATACGCGGTCAGCAAGTCAAAt                                                               >  1:1240256/1‑46 (MQ=255)

                            gTATGGTGCGCTTAACTCATCAACATACGCGGTCAGCAAGTCAAAt                                                               >  1:1326042/1‑46 (MQ=255)

                            gTATGGTGCGCTTAACTCATCAACATACGCGGTCAGCAAGTCAAATTTCAGGGCTGTGTc                                                 <  1:1305105/60‑1 (MQ=255)

                            gTATGGTGCGCTTAACTCATCAACATACGCGGTCAGCAAGTCAAATTTCAGGGCTGTGTc                                                 <  1:184560/60‑1 (MQ=255)

                             tttGGTGCGCTTAACTCATCAACATACGCGGTCAGCAGGTCAAATTTCAGGGCTGTGTCGGGGGCTTCca                                      <  1:1170542/68‑1 (MQ=255)

                               tGGTGCGCTTAACTCATCAACATACGCGGTCAGCAGGTCAAATTTCAGGGCt                                                      <  1:898606/52‑1 (MQ=255)

                                 gTGCGCTTAACTCATCAACATACGCGGTCAGCAGGTCAAATTTCAGGGCTGTGTCGGt                                              >  1:2759859/1‑58 (MQ=255)

                                 gTGCGCTTAACTCATCAACATACGCGGTCAGCAGGTCAAATTTCAGGGCTGTGTCGGTGGCTTCCACAGCg                                 >  1:825348/1‑71 (MQ=255)

                                 gTGCGCTTAACTCATCAACATACGCGGTCAGCAAGTCAAATTTCAGGGCtg                                                     >  1:940529/1‑51 (MQ=255)

                                  tGCGCTTAACTCATCAACATACGCGGTCAGCAGGTCAAAtt                                                              <  1:289290/41‑1 (MQ=255)

                                  tGCGCTTAACTCATCAACATACGCGGTCAGCAGGTCAAAtt                                                              <  1:3553712/41‑1 (MQ=255)

                                  tGCGCTTAACTCATCAACATACGCGGTCAGCAGGTCAAATTTCAGGGCTGTGTCGGTGGCTACca                                      >  1:1160608/1‑65 (MQ=255)

                                    cgcTTAACTCATCAACATACGCGGTCAGCAAGTCAAAtt                                                              <  1:2797623/39‑1 (MQ=255)

                                    cgcTTAACTCATCAACATACGCGGTCAGCAAGTCAAATTTCAGGGCTGTGTCGGTGGCTTCCACAGCGTcc                              >  1:330789/1‑71 (MQ=255)

                                    cgcTTAACTCATCAACATACGCGGTCAGCAAGTCAAATTTCAGGGCTGTGTCGGTGGCTTCCACAGCGTcc                              >  1:1310610/1‑71 (MQ=255)

                                     gcTTAACTCATCAACATACGCGGTCAGCAGGTCAAATTTCAGGGCTGTGTCGGTGGCTTCCACAGCGTCcg                             <  1:2582059/71‑1 (MQ=255)

                                     gcTTAACTCATCAACATACGCGGTCAGCAGGTCAAATTTCAGGGCTGTGTCGGTGGCTTCCACAGCGTCcg                             <  1:1426784/71‑1 (MQ=255)

                                      cTTAACTCATCAACATACGCGGTCAGCAGGTCAAATTTCAGGGCTGTGTCGGTg                                             >  1:377276/1‑54 (MQ=255)

                                      cTTAACTCATCAACATACGCGGTCAGCAGGTCAAATTTCAGGGCTGTGTCGGTGGCTTcc                                       <  1:284295/60‑1 (MQ=255)

                                      cTTAACTCATCAACATACGCGGTCAGCAAGTCAAATTTCAGGGc                                                       <  1:1330830/44‑1 (MQ=255)

                                       ttAACTCATCAACATACGCGGTCAGCAGGTCAAATTTCAGGGCTGTGTCGGTGGCTTCCACAGCGTCcggc                           >  1:511906/1‑71 (MQ=255)

                                        tAACTCATCAACATACGCGGTCAGCAAGTCAAATTTCAGGGc                                                       <  1:538828/42‑1 (MQ=255)

                                         aaCTCATCAACATACGCGGTCAGCAGGTCAAATTTCAggg                                                        >  1:2574728/1‑40 (MQ=255)

                                         aaCTCATCAACATACGCGGTCAGCAGGTCAAATTTCAGGGCTGTGTCGGTgg                                            >  1:1139908/1‑52 (MQ=255)

                                         aaCTCATCAACATACGCGGTCAGCAGGTCAAATTTCAGGGCTGTGTCGGTGGCt                                          >  1:2559470/1‑54 (MQ=255)

                                         aaCTCATCAACATACGCGGTCAGCAGGTCAAATTTCAGGGCTGTGTCGGTGGCTTCCACAGCGTCcggcg                          <  1:214504/70‑1 (MQ=255)

                                          aCTCATCAACATACGCGGTCAGCAGGTCAAATTTCAGGGCTGCGTCGGTGGCTTCCACAGCGTCCGGCGGa                        <  1:2592227/71‑1 (MQ=255)

                                           cTCATCAACATACGCGGTCAGCAGGTCAAATTTCAGGGCTGTGTCGGTGGCTTCCACAGCGTCcggcg                          <  1:3044411/68‑1 (MQ=255)

                                              atcaACATACGCGGTCAGCAGGTCAAATTTCAGGGCTGTGTCGGGGGCTTCCACAGCGTCCGGCGGACAAt                    <  1:3476388/71‑1 (MQ=255)

                                               tcaACATACGCGGTCAGCAGGTCAAATTTCAGGGCTGTGTCGGTGGCTTCCACAGCGTCcgg                            <  1:3039874/62‑1 (MQ=255)

                                               tcaACATACGCGGTCAGCAGGTCAAATTTCAGGGCTGTGTCGGTGGCTTCCACAGCGTCCGGCGGACAATa                   <  1:985489/71‑1 (MQ=255)

                                                 aaCATACGCGGTCAGCAGGTCAAATTTCAGGGCtg                                                     >  1:58422/1‑35 (MQ=255)

                                                 aaCATACGCGGTCAGCAGGTCAAATTTCAGGGCtg                                                     >  1:255305/1‑35 (MQ=255)

                                                 aaCATACGCGGTCAGCAGGTCAAATTTCAGGGCTGTGTCGGTGGCTTCCACAg                                   >  1:2701474/1‑53 (MQ=255)

                                                 aaCATACGCGGTCAGCAGGTCAAATTTCAGGGCTGTGTCGGTGGCTTCCACAg                                   >  1:2256537/1‑53 (MQ=255)

                                                 aaCATACGCGGTCAGCAGGTCAAATTTCAGGGCTGTGTCGGTGGCTTCCACAGCGTCCGGCGGACAAt                    >  1:2865575/1‑68 (MQ=255)

                                                  aCATACGCGGTCAGCAGGTCAAATTTCAGGGCTGTGTCGGTGGCTTCCACAGCGTCCGGCGGACAATAAg                 >  1:118657/1‑70 (MQ=255)

                                                  aCATACGCGGTCAGCAGGTCAAATTTCAGGGCTGTGTCGGTGGCTTCCACAGCGTCCGGCGGACAATAAGa                <  1:836062/71‑1 (MQ=255)

                                                   cATACGCGGTCAGCAGGTCAAATTTCAGGGCTGTGTCGGTGGCTTCca                                      <  1:2366409/48‑1 (MQ=255)

                                                   cATACGCGGTCAGCAAGTCAAATTTCAGGGCTGTGTCg                                                <  1:1325188/38‑1 (MQ=255)

                                                    aTACGCGGTCAGCAGGTCAAATTTCAGGGCTGTGTCGGTGGCTTCCACAGCGTc                               >  1:3902517/1‑54 (MQ=255)

                                                    aTACGCGGTCAGCAGGTCAAATTTCAGGGCTGTGTCGGTGGCTTCCACAGCGTc                               >  1:3865202/1‑54 (MQ=255)

                                                     tACGCGGTCAGCAGGTCAAATTTCAGGGCTGTGTCGGTg                                             <  1:2469156/39‑1 (MQ=255)

                                                       cgcgGTCAGCAGGTCAAATTTCAGGGCTGTGTCGGTGGCTTCCACAGCGTCCGGCGGACAATAAGa                >  1:3264265/1‑66 (MQ=255)

                                                           gTCAGCAGGTCAAATTTCAGGGCTGTGTCGGTGGCTTCCACAGCGTCCGGCGGACAATAAGAAACGCcaca       <  1:1307500/71‑1 (MQ=255)

                                                           gTCAGCAGGTCAAATTTCAGGGCTGTGTCGGTGGCTTCCACAGCGTCCGGCGGACAATAAGAAACGCcaca       >  1:935044/1‑71 (MQ=255)

                                                            tCAGCAGGTCAAATTTCAGGGCTGTGTCGGTGGCTTCCACAGCGTCCGGCGGACAATAAGAAACGCCACAt      <  1:608878/71‑1 (MQ=255)

                                                              agcagGTCAAATTTCAGGGCTGTGTCGGTGGCTTCCACAGCGTCCGGCGGACAATAAGAAACGCCACATTc    <  1:689240/71‑1 (MQ=255)

                                                                cagGTCAAATTTCAGGGCTGTGTCGGTGGCTTCCACAGCGTCCGGCGGACAATAAGAAACGCCACATTCAg  <  1:3660817/71‑1 (MQ=255)

                                                                  ggTCAAATTTCAGGGCTGTGTCGGTGGCTTCCACAGCGTCcg                             <  1:3118933/42‑1 (MQ=255)

TAACAAAATCGATCTCTGCATCTTCCAGGTATGGTGCGCTTAACTCATCAACATACGCGGTCAGCAGGTCAAATTTCAGGGCTGTGTCGGTGGCTTCCACAGCGTCCGGCGGACAATAAGAAACGCCACATTCAG  >  minE/2675229‑2675363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A