Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F21 I0 R2
|
316 |
65.6 |
5147759 |
97.4% |
5013917 |
60.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
257,873 |
A→G |
24.6% |
K15K (AAA→AAG) |
crl → |
DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 257,873 | 0 | A | G | 24.6%
| 100.8
/ 36.3
| 57 | K15K (AAA→AAG) | crl | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (24/19); new base G (6/8); total (30/27) |
| Fisher's exact test for biased strand distribution p-value = 5.40e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 |
CAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCT > W3110S.gb/257803‑257941
|
cAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCaaaaaa < 1:2152595/71‑1 (MQ=255)
gTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAAt > 1:2913965/1‑70 (MQ=255)
ttGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAAttt < 1:849580/71‑1 (MQ=255)
tGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAAttt > 1:2765204/1‑70 (MQ=255)
gCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAAttt > 1:4848915/1‑69 (MQ=255)
tCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGc < 1:2643783/71‑1 (MQ=255)
acaacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCAc > 1:4315255/1‑71 (MQ=255)
acaacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCAc > 1:29592/1‑71 (MQ=255)
acaacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCAc > 1:1347119/1‑71 (MQ=255)
acaacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCAc > 1:4442179/1‑71 (MQ=255)
acaacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCAc < 1:4436345/71‑1 (MQ=255)
acaacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCAc < 1:2971650/71‑1 (MQ=255)
acaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCAc < 1:1577827/68‑1 (MQ=255)
aGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTa > 1:2028349/1‑68 (MQ=255)
gaTAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTAc < 1:871663/57‑1 (MQ=255)
aTAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCg < 1:1111752/69‑1 (MQ=255)
aTAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGta < 1:1805761/71‑1 (MQ=255)
aTAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGta < 1:1322813/71‑1 (MQ=255)
aGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGtata > 1:4814511/1‑71 (MQ=255)
aGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGtata > 1:420628/1‑71 (MQ=255)
aGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGtata > 1:2583770/1‑71 (MQ=255)
aGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGtata > 1:2024303/1‑71 (MQ=255)
cAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCaaaaaa > 1:1906928/1‑47 (MQ=255)
aCGTTACCGAGTGGACACCCGGAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTg < 1:712350/70‑1 (MQ=255)
aCGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCAc > 1:1433922/1‑52 (MQ=255)
gTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAg > 1:1143737/1‑71 (MQ=255)
ttACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTAc < 1:4474392/44‑1 (MQ=255)
ttACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGc < 1:8082/47‑1 (MQ=255)
aCCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGTATATTCGTGAAGGt < 1:3508095/70‑1 (MQ=255)
aCCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCa > 1:1639980/1‑46 (MQ=255)
aCCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGt > 1:4333999/1‑64 (MQ=255)
aCCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAgg > 1:1766416/1‑69 (MQ=255)
aCCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGt < 1:453274/70‑1 (MQ=255)
ccGAGTGGACACCCGAAGAGCAGATTGATCaaaaaa < 1:5027164/36‑1 (MQ=255)
ccGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTa < 1:3360725/70‑1 (MQ=255)
gACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCaa < 1:3424255/70‑1 (MQ=255)
acacCCGAAGAGCAGATTGATCAAAAAGTTTACCg < 1:3775333/35‑1 (MQ=255)
cacCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGtata > 1:2892382/1‑49 (MQ=255)
cacCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGa < 1:1090905/71‑1 (MQ=255)
cccGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGa < 1:1841357/54‑1 (MQ=255)
cGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAg > 1:4315422/1‑54 (MQ=255)
aaGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCg < 1:4394868/71‑1 (MQ=255)
gagCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGAt > 1:855837/1‑71 (MQ=255)
cAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGt > 1:1330242/1‑43 (MQ=255)
cAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGt > 1:4399928/1‑43 (MQ=255)
aGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGAt < 1:1346279/60‑1 (MQ=255)
aGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGAt < 1:575862/60‑1 (MQ=255)
gATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCttt > 1:534152/1‑71 (MQ=255)
gATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCttt > 1:1681172/1‑71 (MQ=255)
aTTGATCAAAAAGTTTACCGCACTAGGCCCGTATATTCGTGAAg < 1:2077916/44‑1 (MQ=255)
aTTGATCAAAAAGTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGa < 1:471685/57‑1 (MQ=255)
aTTGATCAAAAAGTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGa < 1:2965618/57‑1 (MQ=255)
aTTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGt < 1:1649728/50‑1 (MQ=255)
tGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTaa > 1:3665616/1‑46 (MQ=255)
tGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTaa > 1:2262668/1‑46 (MQ=255)
tGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGc > 1:1459890/1‑50 (MQ=255)
tGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTc > 1:4051291/1‑71 (MQ=255)
tGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTc > 1:283536/1‑71 (MQ=255)
aTCAAAAAGTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGa < 1:1370589/71‑1 (MQ=255)
cAAAAAATTTACCGCACTAGGCCCGTATATTCGTg > 1:333928/1‑35 (MQ=255)
aaaaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTg < 1:1596701/71‑1 (MQ=255)
aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGa > 1:1116871/1‑68 (MQ=255)
aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGAt > 1:5016235/1‑69 (MQ=255)
aaaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTg < 1:3940715/70‑1 (MQ=255)
aaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTc < 1:1107880/65‑1 (MQ=255)
aaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTc < 1:3693695/65‑1 (MQ=255)
aaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGt < 1:355908/71‑1 (MQ=255)
aaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCt < 1:534452/71‑1 (MQ=255)
|
CAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCT > W3110S.gb/257803‑257941
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
