Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R2
|
370 |
72.1 |
3738297 |
91.5% |
3420541 |
62.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,434,773 |
T→C |
48.7% |
A465A (GCA→GCG) |
yojI ← |
fused predicted multidrug transport subunits and membrane component and ATP‑binding component of ABC superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,434,773 | 0 | T | C | 48.7%
| ‑2.3
/ 54.3
| 39 | A465A (GCA→GCG) | yojI | fused predicted multidrug transport subunits and membrane component and ATP‑binding component of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base T (11/9); new base C (9/10); total (20/19) |
| Fisher's exact test for biased strand distribution p-value = 7.52e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AAAACTCACGACGGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATACGCCCG > minE/1434703‑1434842
|
aaaaCTCACGACGGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTtcttct > 1:2341957/1‑71 (MQ=255)
aaaaCTCACGACGGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTtcttct > 1:1855574/1‑71 (MQ=255)
aCTCACGACGGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTgcc < 1:3063575/71‑1 (MQ=255)
acgacgGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCCgccagcg > 1:592247/1‑71 (MQ=255)
cgacgGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTgccagcgc < 1:1473491/71‑1 (MQ=255)
acgGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTgccagcgc > 1:626413/1‑69 (MQ=255)
acgGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCCgccagcgcca < 1:3278529/71‑1 (MQ=255)
cgGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCcgccagcgccag > 1:1926044/1‑71 (MQ=255)
gtgtGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCCGCCAGCGCCAGCaa > 1:2388169/1‑69 (MQ=255)
gtgtGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCCGCCAGCGCCAGCAACa < 1:78968/71‑1 (MQ=255)
tgGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAgcg > 1:3684295/1‑71 (MQ=255)
tgGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAgc < 1:1201628/70‑1 (MQ=255)
ccTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCCGCCAGCGCCAGCAACAGCGCCAc > 1:1883338/1‑70 (MQ=255)
tCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTgccagcgccagc < 1:3350898/54‑1 (MQ=255)
tCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGt > 1:36391/1‑69 (MQ=255)
tCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGt > 1:1396900/1‑69 (MQ=255)
tCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCCGCCAGCGCCAGCAACAGCGCCACGCGttt < 1:41755/71‑1 (MQ=255)
gccgccCATTCATCCAGCAGGATAATATCGCGTTCTTCCGCCAGCGCCAGCAACAGCGCCACGCGtttttt < 1:3125488/71‑1 (MQ=255)
ccgccCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTc < 1:3709886/71‑1 (MQ=255)
cgccCATTCATCCAGCAGGATAATATCGCGTTCTTCCGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCt > 1:1429297/1‑71 (MQ=255)
aTTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCt > 1:709712/1‑71 (MQ=255)
aTTCATCCAGCAGGATAATATCGCGTTCTTCCGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCt < 1:556907/71‑1 (MQ=255)
aTTCATCCAGCAGGATAATATCGCGTTCTTCCGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCt < 1:1458767/71‑1 (MQ=255)
ttCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCtt < 1:1759443/71‑1 (MQ=255)
cATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCtttt > 1:3021820/1‑71 (MQ=255)
cATCCAGCAGGATAATATCGCGTTCTTCCGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCtttt > 1:3518989/1‑71 (MQ=255)
cATCCAGCAGGATAATATCGCGTTCTTCCGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCtttt > 1:1307678/1‑71 (MQ=255)
agcagGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTg > 1:781621/1‑67 (MQ=255)
agcagGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATaa > 1:1256479/1‑71 (MQ=255)
agGATAATATCGCGTTCTTCCGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATaa > 1:3366865/1‑68 (MQ=255)
ggATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCttt < 1:2712021/61‑1 (MQ=255)
gATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACtt < 1:1426877/69‑1 (MQ=255)
gATAATATCGCGTTCTTCCGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCa < 1:2882187/71‑1 (MQ=255)
taataTCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAgg > 1:2932208/1‑71 (MQ=255)
taataTCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAgg > 1:2179624/1‑71 (MQ=255)
taataTCGCGTTCTTCCGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCacg > 1:124341/1‑69 (MQ=255)
aataTCGCGTTCTTCCGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCtttt < 1:1750003/58‑1 (MQ=255)
atatCGCGTTCTTCCGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGtt < 1:3013724/71‑1 (MQ=255)
tatCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTa < 1:276406/71‑1 (MQ=255)
tatCGCGTTCTTCCGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTa < 1:1306978/71‑1 (MQ=255)
gcgTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACaa > 1:867669/1‑71 (MQ=255)
ttcttcCGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCt > 1:2979089/1‑41 (MQ=255)
tcttccGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATACg > 1:201973/1‑71 (MQ=255)
cttccGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATACGc > 1:929458/1‑71 (MQ=255)
ttctGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTa < 1:406964/61‑1 (MQ=255)
ttccGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAAc < 1:2239760/63‑1 (MQ=255)
tctGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATACGc < 1:1961052/69‑1 (MQ=255)
ctGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATACGCCCg > 1:1637396/1‑71 (MQ=255)
|
AAAACTCACGACGGAAGTGTGGATCCTGATCCGCCGCCCATTCATCCAGCAGGATAATATCGCGTTCTTCTGCCAGCGCCAGCAACAGCGCCACGCGTTTTTTCTGCCCTTTTGATAACTTCAGGTTAACAATACGCCCG > minE/1434703‑1434842
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
