Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,564,912 |
G→A |
76.3% |
intergenic (‑124/+168) |
yfdE ← / ← glk |
predicted CoA‑transferase, NAD(P)‑binding/glucokinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,564,912 | 0 | G | A | 76.3%
| 130.0
/ 39.2
| 76 | intergenic (‑124/+168) | yfdE/glk | predicted CoA‑transferase, NAD(P)‑binding/glucokinase |
| Reads supporting (aligned to +/- strand): ref base G (11/7); new base A (29/29); total (40/36) |
| Fisher's exact test for biased strand distribution p-value = 4.34e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATAACATGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGT > minE/1564849‑1564978
|
aTAACATGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACa < 1:1394095/68‑1 (MQ=255)
aaCATGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACaaa > 1:1090873/1‑68 (MQ=255)
cATGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGcc > 1:3983791/1‑71 (MQ=255)
tGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCg > 1:1351107/1‑67 (MQ=255)
atcGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTAc > 1:5665730/3‑69 (MQ=255)
atcGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTAc > 1:13374/3‑69 (MQ=255)
gTTAAATCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAAt > 1:989525/1‑57 (MQ=255)
ttAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTa < 1:4799263/64‑1 (MQ=255)
ttAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGc > 1:2518740/1‑59 (MQ=255)
aaTTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACaaa > 1:745230/1‑71 (MQ=255)
aaTTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACaaa > 1:1866222/1‑71 (MQ=255)
aTTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAaat < 1:3780505/71‑1 (MQ=255)
aTTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAaat < 1:620533/71‑1 (MQ=255)
aTTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAaat < 1:4329885/71‑1 (MQ=255)
aTTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAaat < 1:3513230/71‑1 (MQ=255)
ccTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTa > 1:3833993/1‑58 (MQ=255)
ccTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACaa < 1:5404833/66‑1 (MQ=255)
ccTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAaataat > 1:3860437/1‑71 (MQ=255)
ccTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAaataat > 1:1453044/1‑71 (MQ=255)
ccTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAaataa > 1:3845153/1‑70 (MQ=255)
ccTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAaataa < 1:4794704/70‑1 (MQ=255)
ccTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAaataat > 1:5530491/1‑71 (MQ=255)
tCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCa < 1:453067/59‑1 (MQ=255)
tCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCa < 1:3466279/59‑1 (MQ=255)
tCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCa < 1:3649770/59‑1 (MQ=255)
tCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCa < 1:1866768/59‑1 (MQ=255)
tCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCa < 1:1181267/59‑1 (MQ=255)
tCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATg > 1:1232916/1‑70 (MQ=255)
ttCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCAtt < 1:2494953/71‑1 (MQ=255)
ttCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCAtt < 1:5621617/71‑1 (MQ=255)
ttCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCAtt < 1:433230/71‑1 (MQ=255)
aGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTa < 1:2541359/70‑1 (MQ=255)
gTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACt < 1:4275528/71‑1 (MQ=255)
tGATAGTTGACGAGTGAAATTATGAACGTGCAAAACaaa < 1:2188321/39‑1 (MQ=38)
tGATAGTTGACGAGTGAAATTATGAACGTGCAAAACaaa < 1:2050402/39‑1 (MQ=38)
gATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTg > 1:4525058/1‑70 (MQ=255)
aTAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGt > 1:4576650/1‑44 (MQ=38)
aTAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAaataa > 1:1927597/1‑58 (MQ=255)
aTAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTg > 1:1972136/1‑69 (MQ=255)
gttgacgaGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGgaga < 1:210699/67‑1 (MQ=255)
gttgacgaGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGgaga < 1:2779091/67‑1 (MQ=255)
tgacgaGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAAt > 1:3404159/2‑70 (MQ=255)
gacgaGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCAttt < 1:5086357/59‑1 (MQ=255)
gacgaGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAAtt > 1:2494595/1‑70 (MQ=255)
gacgaGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTa < 1:3564703/71‑1 (MQ=255)
gacgaGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAAtt > 1:4805922/1‑70 (MQ=255)
acgaGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTaa > 1:2720629/1‑71 (MQ=255)
acgaGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTaa < 1:2357501/71‑1 (MQ=255)
acgaGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTaa < 1:934415/71‑1 (MQ=255)
ctaGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGgaga < 1:2761392/63‑1 (MQ=255)
tGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAaata > 1:5284013/1‑45 (MQ=255)
tGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCg > 1:5608278/1‑71 (MQ=255)
gAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGc > 1:3449051/1‑71 (MQ=255)
aaaTTATGAACGTGCAGAACAAATCGCCGTTACCAGACAaat > 1:238978/1‑42 (MQ=255)
aaaTTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCt > 1:1245752/1‑71 (MQ=255)
aaaTTATGAACGTGCAAAACAAATCGCCGTTACCAGACAaataa > 1:5503957/1‑44 (MQ=255)
aaaTTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTa > 1:1244948/1‑52 (MQ=255)
aTTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGgaga < 1:4067804/57‑1 (MQ=255)
aTTATGAACGTGCAAAACAAATCGCCGTTACCAGACAaata > 1:2916374/1‑41 (MQ=255)
aTTATGAACGTGCAAAACAAATCGCCGTTACCAGACAaata > 1:3826324/1‑41 (MQ=255)
aTTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTg > 1:4397406/1‑70 (MQ=255)
tATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTAc < 1:4301292/49‑1 (MQ=255)
tATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTAc < 1:5383225/49‑1 (MQ=255)
tATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTAc < 1:4165229/49‑1 (MQ=255)
aTGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTa > 1:3071688/1‑47 (MQ=255)
aTGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTa > 1:3426928/1‑47 (MQ=255)
aTGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCt > 1:906641/1‑66 (MQ=255)
aTGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCt > 1:2168664/1‑66 (MQ=255)
aTGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCt > 1:3120076/1‑66 (MQ=255)
tGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTg > 1:4545645/1‑49 (MQ=255)
tGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACgg < 1:5235651/71‑1 (MQ=255)
gAACGTGCAAAACAAATCGCCGTTACCAGACAaataa < 1:337291/37‑1 (MQ=255)
aCGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAg > 1:5247916/1‑71 (MQ=255)
aCGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAg < 1:678046/71‑1 (MQ=255)
gCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAgtgt < 1:4726409/70‑1 (MQ=255)
gCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAgtgt < 1:3085498/70‑1 (MQ=255)
|
ATAACATGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGT > minE/1564849‑1564978
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
